CN201990663U - PCR (polymerase chain reaction) tube - Google Patents
PCR (polymerase chain reaction) tube Download PDFInfo
- Publication number
- CN201990663U CN201990663U CN2011200722601U CN201120072260U CN201990663U CN 201990663 U CN201990663 U CN 201990663U CN 2011200722601 U CN2011200722601 U CN 2011200722601U CN 201120072260 U CN201120072260 U CN 201120072260U CN 201990663 U CN201990663 U CN 201990663U
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- pcr
- tube
- temperature
- reaction tubes
- reaction
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Abstract
The utility model relates to a PCR (polymerase chain reaction) tube, which solves problems that existing widely used PCR tubes are mostly made of plastic and poor in heat conductivity, temperature of reacted liquor in tubes and temperature of a heater of a PCR heat circulator are inconsistent, reacting time is prolonged, experiment efficiency is reduced, and a scheme of blindly reducing the thickness of tube walls for solving the above problems is nonoptimal. Metal layers are covered on the inner side and the outer side of the PRC tube and are communicated with each other, so that the reacted liquor in the PCR tube is communicated with the heater of the PCR heat circulator by the aid of the metal layers, and temperature of the reacted liquor in the PCR tube and temperature of the heater of the PCR heat circulator are consistent. Heat conduction speed of the PCR tube is effectively improved, reaction time of a PCR can be shortened by 30% to 60% while reaction effectiveness is guaranteed.
Description
Technical field
The utility model relates to a kind of PCR reaction tubes.
Background technology
The PCR reaction tubes is a reaction vessels that the most generally uses during pcr amplification reaction is implemented, a typical pcr amplification reaction is by the PCR thermal cycler heating and cooling that the PCR pipe that is placed on wherein carries out repeatedly to be finished, the conduction of temperature is conducted to the PCR reaction tubes that is placed on wherein by 96 hole heatable metal block of PCR thermal cycler in this process, tube wall through the PCR reaction tubes conducts to the interior PCR reaction solution of tube wall then, thereby causes amplified reaction.Whether PCR reaction can increase except the composition of gene template quality, polysaccharase character, reaction solution to gene template quickly and efficiently, and the speed of the heating and cooling of reaction solution also is The key factor very.The efficient of the fast more PCR reaction of warming and cooling rate is just high more, is not easy to produce non-purpose amplified production more; The what is more important heating and cooling are fast more, can shorten the time of PCR reaction greatly, and this is significant in clinical diagnostic nucleic acid.
Present widely used PCR reaction tubes all is to adopt the material of plastic nature injection molded, and the heat conductivility of plastics itself is relatively poor.After tested, when the heating system of PCR instrument reaches 94 ℃ temperature, the temperature of the reaction solution in the PCR reaction tubes only is about 86 ℃, only to 15 second afterreaction liquid temperature just can reach 94 ℃ of pulling temperatures, reduce the also best absolutely not improvement project of thickness of tube wall simply.
Summary of the invention
The purpose of this utility model provides a kind of PCR reaction tubes that can effectively improve heat conduction velocity, reduce the PCR reaction times.
The technical scheme that the utility model adopted is:
A kind of PCR reaction tubes includes reaction tubes, it is characterized in that: the inside and outside both sides of described reaction tubes tube wall are coated with metal level.
Described metal level is copper layer or aluminium lamination.
The inside and outside both sides of described reaction tubes tube wall are coated with the inside and outside connection of metal level.
Described metal level is all to cover or the part covering.
The utlity model has following advantage:
The utility model is communicated with metal level the inside and outside both sides that cover the PCR reaction tubes, the interior reaction solution of PCR reaction tubes and the heating unit of PCR thermal cycler are connected by metal level, it is highly consistent that thereby the temperature variation that makes both keeps, effectively improved the heat conduction velocity of PCR reaction tubes, not only the reaction times of a PCR can be shortened to 40 minutes from 90 minutes, also effectively guarantee the accuracy of reaction result.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Among the figure, 1-PCR reaction tubes, 2-metal level.
Embodiment
Below in conjunction with embodiment the utility model is described in detail.
The utility model is communicated with covering by electro-plating method with the interior outside of the PCR reaction tubes 1 of 200ml, form inside and outside two metal layers 2, and with two metal layers 2 connections, the reaction solution in the reaction tubes 1 and the heating unit of PCR thermal cycler are connected by metal level 2, it is highly consistent that thereby the temperature variation that makes both keeps, the time of finishing a common PCR reaction is shortened to 40 minutes from 90 minutes, and do not influence reaction result.Metal level can select for use copper, aluminium etc. to have the material of good big thermal characteristics.
Metal level can adopt whole coverings or part to cover this dual mode to the covering of reaction tubes 1 inside and outside tube wall, the purpose that part covers is under the prerequisite of the heat conductivility that guarantees PCR reaction tubes 1, save metallic substance, but the part area coverage should be not less than 1 15.
Claims (4)
1. a PCR reaction tubes includes reaction tubes (1), it is characterized in that: the inside and outside both sides of described reaction tubes (1) tube wall are coated with metal level (2).
2. a kind of PCR reaction tubes according to claim 1 is characterized in that: described metal level (2) is copper layer or aluminium lamination.
3. a kind of PCR reaction tubes according to claim 1 is characterized in that: the inside and outside both sides of described reaction tubes (1) tube wall are coated with the inside and outside connection of metal level (2).
4. a kind of PCR reaction tubes according to claim 1 is characterized in that: described metal level (2) is for all covering or the part covering.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200722601U CN201990663U (en) | 2011-03-18 | 2011-03-18 | PCR (polymerase chain reaction) tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200722601U CN201990663U (en) | 2011-03-18 | 2011-03-18 | PCR (polymerase chain reaction) tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201990663U true CN201990663U (en) | 2011-09-28 |
Family
ID=44667157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011200722601U Expired - Fee Related CN201990663U (en) | 2011-03-18 | 2011-03-18 | PCR (polymerase chain reaction) tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201990663U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701560A (en) * | 2016-06-13 | 2017-05-24 | 秦荣 | PCR test tube |
| CN106755457A (en) * | 2017-01-09 | 2017-05-31 | 杭州安杰思生物科技有限公司 | A kind of PCR detection method of use metal test tubes |
-
2011
- 2011-03-18 CN CN2011200722601U patent/CN201990663U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701560A (en) * | 2016-06-13 | 2017-05-24 | 秦荣 | PCR test tube |
| CN106755457A (en) * | 2017-01-09 | 2017-05-31 | 杭州安杰思生物科技有限公司 | A kind of PCR detection method of use metal test tubes |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110928 Termination date: 20120318 |