CN106867892A - A kind of metal test tubes PCR instrument - Google Patents
A kind of metal test tubes PCR instrument Download PDFInfo
- Publication number
- CN106867892A CN106867892A CN201710013029.7A CN201710013029A CN106867892A CN 106867892 A CN106867892 A CN 106867892A CN 201710013029 A CN201710013029 A CN 201710013029A CN 106867892 A CN106867892 A CN 106867892A
- Authority
- CN
- China
- Prior art keywords
- test tubes
- metal test
- pcr instrument
- metal
- rate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The invention discloses a kind of metal test tubes PCR instrument, the PCR instrument uses metal test tubes.The present invention replaces plastic test tube using metal test tubes, heat conductivility is remarkably reinforced, improve the reagent rate of heat addition, greatly increase amplification efficiency and heating uniformity, particularly to reagent that some are required to rate of amplification, traditional approach cannot meet requirement using plastic test tube, and after using metal test tubes, can improve detection rates and accuracy of detection.
Description
Technical field
The invention belongs to field of analytic instrument, and in particular to a kind of metal test tubes PCR instrument.
Background technology
Molecule diagnosis refers to the diagnostic techniques for carrying out clinical detection as biomarker using nucleic acid or protein, is disease
Prediction, diagnose, prevent, treat and lapse to there is provided information and decision-making foundation.Particularly face various sudden infectiousness diseases
Disease, its most economical effective measures are exactly fast and accurately molecule diagnosis.Current conventional molecular diagnoses PCR instrument for reagent
Amplification is basic to carry out augmentation detection using plastic test tube, and because plastic test tube firing rate is slow and heating uniformity is poor, influence is expanded
Speedup rate, so as to influence detection speed and accuracy of detection.
The content of the invention
Based on above mentioned problem, the present invention provides a kind of metal test tubes PCR instrument, replaces conventional plastics to try using metal test tubes
Pipe, when performing PCR detection is entered, the heat conductivility of metal test tubes is remarkably reinforced, so that amplification efficiency is greatly increased and heated
Even property is greatly improved, and particularly to the reagent that some are required to rate of amplification, traditional approach cannot be expired using plastic test tube
Foot is required, and can finally improve detection rates and accuracy of detection using metal test tubes.
The technical solution adopted by the present invention is：A kind of metal test tubes PCR instrument, it is characterised in that the PCR instrument uses metal
Test tube.
Further, the size specification of the metal test tubes is adapted to PCR instrument.
Further, the metal test tubes are using the good metal material of heat conductivility, the thermal conductivity factor of metal material, typically
In more than 10W/m.K, the metal material that preferably present invention is used is for more than thermal conductivity factor 50W/m.K.Compared with metal material, modeling
Expect that the heat conductivility of material is very poor, the best HDPE thermal conductivity factors of thermal conductivity also only have 0.44W/m.K, typically all 0.1~
Between 0.3W/m.K.
Further, the metal material preferably silver, copper, aluminium, magnesium, zinc and its alloy, such as aluminium alloy, almag, copper
Alloy, ormolu etc..
The present invention can enter performing PCR and detect using the metal test tubes PCR instrument, and PCR amplifing reagents are added into metal test tubes,
Metal test tubes are put into PCR instrument, amplification program is set in PCR instrument carries out reagent amplification.
The beneficial effects of the present invention are：Replace plastic test tube, heat conductivility to be remarkably reinforced using metal test tubes, improve
The reagent rate of heat addition, 5~10 times are improved than the rate of heat addition using plastic test tube, greatly shorten the heat time of PCR amplifications, are carried
Amplification efficiency high and heating uniformity, particularly to the reagent that some are required to rate of amplification, traditional approach is tried using plastics
Pipe cannot meet requirement, and after using metal test tubes, can improve detection rates and accuracy of detection.
Specific embodiment
Technical scheme is described further with specific embodiment below, but protection scope of the present invention
Not limited to this.
Embodiment 1
By PCR amplifing reagents (DNA profiling to be measured, primer, Taq archaeal dna polymerases, dNTPs, buffer solution etc., the μ of cumulative volume 50
L) add in metal test tubes, metal test tubes are aluminium alloy test tube, size specification is identical with former plastics EP test tubes.Metal test tubes are put
Enter in PCR instrument, setting amplification program carries out reagent amplification in PCR instrument.Actual measurement average heating rate is 15 DEG C/s, equal conditions
Under, it is 2.4 DEG C/s to use plastic test tube to survey average heating rate.
Therefore can significantly shorten the heat time using metal test tubes, improve the detection rates of PCR amplifications.
Claims (6)
1. a kind of metal test tubes PCR instrument, it is characterised in that the PCR instrument uses metal test tubes.
2. metal test tubes PCR instrument as claimed in claim 1, it is characterised in that the size specification and PCR instrument of the metal test tubes
Adaptation.
3. metal test tubes PCR instrument as claimed in claim 1, it is characterised in that metal material that the metal test tubes are used is led
Hot coefficient is in more than 10W/m.K.
4. metal test tubes PCR instrument as claimed in claim 3, it is characterised in that metal material that the metal test tubes are used is led
Hot coefficient is in more than 50W/m.K.
5. metal test tubes PCR instrument as claimed in claim 4, it is characterised in that the metal material that the metal test tubes are used for
Silver, copper, aluminium, magnesium, zinc and its alloy.
6. metal test tubes PCR instrument as claimed in claim 5, it is characterised in that the metal material that the metal test tubes are used is aluminium
Alloy, almag, copper alloy or ormolu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710013029.7A CN106867892A (en) | 2017-01-09 | 2017-01-09 | A kind of metal test tubes PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710013029.7A CN106867892A (en) | 2017-01-09 | 2017-01-09 | A kind of metal test tubes PCR instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106867892A true CN106867892A (en) | 2017-06-20 |
Family
ID=59164777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710013029.7A Pending CN106867892A (en) | 2017-01-09 | 2017-01-09 | A kind of metal test tubes PCR instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106867892A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004027023A2 (en) * | 2002-09-17 | 2004-04-01 | Pharmacia Corporation | Preservation of rna and reverse transcriptase during automated liquid handling |
| CN201258329Y (en) * | 2008-09-02 | 2009-06-17 | 杭州博日科技有限公司 | Fluorescent quantitative PCR test-tube heating sealing device |
| CN201981185U (en) * | 2011-04-01 | 2011-09-21 | 杭州金源生物技术有限公司 | High-speed polymerase chain reaction (PCR) tube |
| CN102471746A (en) * | 2009-08-20 | 2012-05-23 | 宝生物工程有限公司 | Temperature cycling device |
| CN203079967U (en) * | 2013-01-28 | 2013-07-24 | 重庆市林业科学研究院 | PCR (Polymerase Chain Reaction) tube |
| CN204848844U (en) * | 2015-06-05 | 2015-12-09 | 秦荣 | PCR test tube and array thereof |
| CN105861290A (en) * | 2016-06-13 | 2016-08-17 | 秦荣 | PCR test tube |
| CN106282327A (en) * | 2015-06-10 | 2017-01-04 | 天津迈科生物技术有限公司 | A kind of method utilizing quantitative real time PCR Instrument to carry out DNA cloning |
-
2017
- 2017-01-09 CN CN201710013029.7A patent/CN106867892A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004027023A2 (en) * | 2002-09-17 | 2004-04-01 | Pharmacia Corporation | Preservation of rna and reverse transcriptase during automated liquid handling |
| CN201258329Y (en) * | 2008-09-02 | 2009-06-17 | 杭州博日科技有限公司 | Fluorescent quantitative PCR test-tube heating sealing device |
| CN102471746A (en) * | 2009-08-20 | 2012-05-23 | 宝生物工程有限公司 | Temperature cycling device |
| CN201981185U (en) * | 2011-04-01 | 2011-09-21 | 杭州金源生物技术有限公司 | High-speed polymerase chain reaction (PCR) tube |
| CN203079967U (en) * | 2013-01-28 | 2013-07-24 | 重庆市林业科学研究院 | PCR (Polymerase Chain Reaction) tube |
| CN204848844U (en) * | 2015-06-05 | 2015-12-09 | 秦荣 | PCR test tube and array thereof |
| CN106282327A (en) * | 2015-06-10 | 2017-01-04 | 天津迈科生物技术有限公司 | A kind of method utilizing quantitative real time PCR Instrument to carry out DNA cloning |
| CN105861290A (en) * | 2016-06-13 | 2016-08-17 | 秦荣 | PCR test tube |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102618646B (en) | Quantitative detection method for gene copy number | |
| CN103740824B (en) | A kind of two Nucleotide synthesize the method for Sequencing chromatogram Identifying micro-organisms population in real time | |
| CN102782674B (en) | Determination of elbow values for PCR for parabolic shaped curves | |
| CN105400904A (en) | RPA kit used for detecting Ebola virus, and special-purpose primers, probes, and applications of RPA kit | |
| CN103966228B (en) | A kind of Rh blood group DEL type RHD93T > A allelotrope and detection method thereof | |
| CN102559898A (en) | Reagent kit and method for detecting human alpha defensin 1/3 gene copy number | |
| CN104894246A (en) | Two-nucleotide synthetic sequencing analysis method for multi-template PCR product | |
| CN110512012A (en) | One group of multiple PCR primer and its application based on specific target identification Listeria Monocytogenes serotype | |
| CN107267605A (en) | The SNP marker related to china holstein cowses reproductive trait and its application | |
| CN106867892A (en) | A kind of metal test tubes PCR instrument | |
| CN103937806B (en) | A kind of Rh blood group DEL type RHD838G > A allelotrope and detection method thereof | |
| CN101570789B (en) | Identification and application of pig MHC II TA gene as immunity related molecular labels | |
| CN106755457A (en) | A kind of PCR detection method of use metal test tubes | |
| CN103525923A (en) | Primer, probe and method for qualitatively and quantitatively detecting witches' broom phytoplasma | |
| CN104313124A (en) | Specific primer for detecting bovine IGF-1 genes, fluorescent quantitative PCR detection kit of bovine GHR genes, and detection method and application of kit | |
| CN1699600A (en) | Primer sequence and kit for real-time PCR detection of transgenic rape (seeds) | |
| Miller et al. | Quantitative polymerase chain reaction | |
| CN104388580A (en) | Method for identifying homozygotic type or heterozygous type NK603 on basis of digital PCR (polymerase chain reaction) | |
| CN104328176A (en) | Rapid direction method for nucleic acid sensor based on PCR | |
| CN107164510A (en) | A kind of detection kit and its detection method of auxiliary diagnosis cerebral apoplexy | |
| Matsenko et al. | Comparison of SYBR Green I and TaqMan real-time PCR formats for the analysis of her2 gene dose in human breast tumors | |
| CN102605088A (en) | Method for rapidly detecting copy number variation of alpha-globin gene cluster | |
| CN102732609B (en) | Method for detecting similarity of oligonucleotide and target genome | |
| CN110475871B (en) | Analytical signal for determining the presence of a target nucleic acid sequence | |
| CN1203187C (en) | Probe sequence and kit for real time fluorescent PCR detection of transgenic corn |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170620 |