CN201258329Y - Fluorescent quantitative PCR test-tube heating sealing device - Google Patents
Fluorescent quantitative PCR test-tube heating sealing device Download PDFInfo
- Publication number
- CN201258329Y CN201258329Y CNU2008201640429U CN200820164042U CN201258329Y CN 201258329 Y CN201258329 Y CN 201258329Y CN U2008201640429 U CNU2008201640429 U CN U2008201640429U CN 200820164042 U CN200820164042 U CN 200820164042U CN 201258329 Y CN201258329 Y CN 201258329Y
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- test tube
- hole
- nut
- lower pressure
- pressure plate
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- Expired - Lifetime
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Abstract
The utility model discloses a device for heating and sealing a fluorescence quantifying PCR test tube. The device comprises an upper and a lower pressure plates, heating elements are respectively inlaid in the upper and lower pressure plates, a first hole for inserting with a test tube is formed on the lower pressure plate, the upper pressure plate is provided with a second hole corresponding to the first hole on the lower pressure plate, the upper pressure plate is fixed with a screw rod, the nut of the screw rod is located in a nut locating plate, and the upper pressure plate is driven by a screw rod nut mechanism to move up and down. the plastic test tube is in melted connection with the edges of the a sealing cove of the test tube by using the upper and the lower pressure plates for heating, thereby the specimen in the test tube is completely sealed. The device can effectively prevent the prepared specimen and environment from being polluted by the amplified production of the PCR with high concentration, and the expensive standard PCR laboratory is not required.
Description
Technical field
The utility model relates to quantitative fluorescent PCR test tube heat seal device.
Background technology
Fluorescence quantitative PCR analyzing system detects in real time and the fluorescence signal intensity of the proportional relation of target gene concentration, and the data processing by software obtains target gene concentration.This fluorescence quantitative PCR analyzing system has very high sensitivity, but very high to operational requirement.All be manual operation when sample prepares, deal with improperly slightly and sample contamination, contrast problem such as make mistakes will occur, in the pcr amplification process subsequently, these wrong can amplifications by geometricprogression, if air is polluted by template,, also can cause the false positive mistake of analytical results even operation is accurate again.Because a large amount of sample process, the chance that operator contact the pathogeny body increases greatly, has certain Biosafety hidden danger, therefore all there is certain requirement in the laboratory of carrying out the PCR detection to environment usually, as needs the PCR Lab of standard.Under the bigger situation of sample size, the workload that artificial sample is handled is very big.Address these problems and will carry out the automatization processing to sample, key point is wherein carried out encapsulation process to invisible spectro amplified sample exactly, avoids the pollution of high density sample to low concentration sample and environment.Present PCR test tube only covers test tube with the test tube sealing cover and seals, but such sealing effectiveness is very poor, and sample can be from evaporation between test tube and the test tube lid.
Summary of the invention
The purpose of this utility model provides a kind of simple and economic quantitative fluorescent PCR test tube heat seal device, with realization invisible spectro sample is sealed fully, stops the pollution of pcr amplification process middle and high concentration amplified production to sample before increasing and environment.
Quantitative fluorescent PCR test tube heat seal device of the present utility model comprises: upper and lower pressing plate, be embedded with Heating element in the upper and lower pressing plate respectively, press table is provided with first hole of plant test tube, top board has and corresponding second hole, press table first hole, top board and screw mandrel are fixed, the feed screw nut is limited in the nut limiting plate, and top board moves up and down under leading screw and nut mechanism drives.
The upper and lower pressing plate of the utility model utilization heating is welded together the test tube of plastics and the edge of test tube sealing cover, thereby the sample in the test tube is sealed fully.
The beneficial effects of the utility model are:
1. seal the amplified sample test tube fully.
2. stop of the pollution of the pcr amplification product of high density, need not use expensive standard PCR Lab preparation sample and environment.
Description of drawings
Fig. 1 is a quantitative fluorescent PCR test tube heat seal device synoptic diagram.
Embodiment
With reference to Fig. 1, quantitative fluorescent PCR test tube heat seal device comprises: upper and lower pressing plate 1,2, in the legend, upper and lower pressing plate adopts aluminium sheet, is embedded with Heating element 3 in upper and lower aluminium sheet respectively, and lower aluminum sheet 2 is provided with first hole 8 of plant test tube 7, top board has and corresponding second hole 9, press table first hole, last aluminium sheet 2 is fixing with screw mandrel 4, and feed screw nut 5 is limited in the nut limiting plate 6, and last aluminium sheet moves up and down under leading screw and nut mechanism drives.
During work, lower aluminum sheet maintains static, plant PCR test tube on it, first bore edges of test tube edge and lower aluminum sheet contacts, the test tube sealing cover is covered on test tube, driving nut by leading screw and nut mechanism rotates, screw mandrel is moved up and down, and aluminium sheet moves up and down thereby drive upward, and the edge, second hole of test tube sealing cover edge and last aluminium sheet contacts, by moving downward of last aluminium sheet test tube sealing cover and test tube are pressed together, on this moment, leave certain clearance between the lower aluminum sheet,, will go up by the heat that adds of control Heating element, lower aluminum sheet is controlled at 100 to 350 ℃, make the test tube and the welding of test tube sealing cover of plastics, the sample in the test tube is effectively sealed.
Claims (2)
1. quantitative fluorescent PCR test tube heat seal device, it is characterized in that comprising upper and lower pressing plate (1,2), upper and lower pressing plate is embedded with Heating element (3) respectively in (1,2), press table (2) is provided with first hole (8) of plant test tube, top board has and corresponding second hole, press table first hole (9), top board (2) is fixing with screw mandrel (4), and feed screw nut (5) is limited in the nut limiting plate (6), and top board moves up and down under leading screw and nut mechanism drives.
2. quantitative fluorescent PCR test tube heat seal device according to claim 1 is characterized in that said upper and lower pressing plate is an aluminium sheet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201640429U CN201258329Y (en) | 2008-09-02 | 2008-09-02 | Fluorescent quantitative PCR test-tube heating sealing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201640429U CN201258329Y (en) | 2008-09-02 | 2008-09-02 | Fluorescent quantitative PCR test-tube heating sealing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201258329Y true CN201258329Y (en) | 2009-06-17 |
Family
ID=40772564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201640429U Expired - Lifetime CN201258329Y (en) | 2008-09-02 | 2008-09-02 | Fluorescent quantitative PCR test-tube heating sealing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201258329Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696443B (en) * | 2009-10-27 | 2013-05-08 | 西安天隆科技有限公司 | Method and device for reducing evaporation and nonspecific reaction in PCR amplification |
| CN106867892A (en) * | 2017-01-09 | 2017-06-20 | 杭州安杰思生物科技有限公司 | A kind of metal test tubes PCR instrument |
| CN108254245A (en) * | 2018-02-08 | 2018-07-06 | 烟台海深威软件有限公司 | A kind of deep-well plates Sample location thawing apparatus |
| CN108855289A (en) * | 2017-05-09 | 2018-11-23 | 广州康昕瑞基因健康科技有限公司 | loading tool |
-
2008
- 2008-09-02 CN CNU2008201640429U patent/CN201258329Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696443B (en) * | 2009-10-27 | 2013-05-08 | 西安天隆科技有限公司 | Method and device for reducing evaporation and nonspecific reaction in PCR amplification |
| CN106867892A (en) * | 2017-01-09 | 2017-06-20 | 杭州安杰思生物科技有限公司 | A kind of metal test tubes PCR instrument |
| CN108855289A (en) * | 2017-05-09 | 2018-11-23 | 广州康昕瑞基因健康科技有限公司 | loading tool |
| CN108254245A (en) * | 2018-02-08 | 2018-07-06 | 烟台海深威软件有限公司 | A kind of deep-well plates Sample location thawing apparatus |
| CN108254245B (en) * | 2018-02-08 | 2023-11-24 | 烟台海深威软件有限公司 | Deep hole plate sample positioning and melting device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20090617 |