CN201981185U

CN201981185U - High-speed polymerase chain reaction (PCR) tube

Info

Publication number: CN201981185U
Application number: CN2011200921450U
Authority: CN
Inventors: 余伟明
Original assignee: HANGZHOU JINYUAN BIOTECH CO Ltd
Current assignee: HANGZHOU JINYUAN BIOTECH CO Ltd
Priority date: 2011-04-01
Filing date: 2011-04-01
Publication date: 2011-09-21
Anticipated expiration: 2021-04-01

Abstract

The utility model relates to a device for polymerase chain reaction (PCR), in particular to a high-speed PCR tube, which comprises a tube body and a tube cover matched with the tube body, wherein the tube body is in an aluminum alloy structure and comprises a tubular main body and a conical bottom connected with the main body, the tube cover and the tube body are in a split structure, and the tube cover is made of polypropylene (PP) materials. The PP materials are polypropylene materials, have good heat conductivility but are easy to deform in the repeated heating process because the heat twisting temperature is very low (100 DEG C). The high-speed PCR tube has the advantages that the PP materials for making the PCR tube are changed into aluminum alloy materials, the heat conductivility is greatly improved, the conversion rate in PCR experiments can be improved by 700 times or even more than 700 times, and the experiment time is greatly shortened.

Description

The high-speed PCR pipe

Technical field

The utility model relates to a kind of PCR reaction and uses device, especially relates to a kind of high-speed PCR pipe.

Background technology

At present, in molecular biology research and medical clinic applications process, the PCR reaction technology is indispensable.The PCR reaction is that (polymerase chain reaction PCR), is a kind of method at external rapid amplifying specific gene or dna sequence dna, so be called the gene in vitro TRAP again in the polymerase chain reaction.Dna segment both sides to be amplified and with two Oligonucleolide primers of its both sides complementary, after several circulations of sex change, annealing and extension, DNA cloning 2 square time doubly.The PCR pipe is used for PCR instrument and sequenator.

Though the PP material thermal conductivity better (thermal conductivity is about 0.2) that traditional PCR pipe is used still needs certain waiting time in the heat-processed repeatedly of experiment.When using conventional P CR pipe to experimentize, the time of single experiment needs two to three hours.As the patent No. is that the disclosed quantitative fluorescent PCR test tube of the Chinese utility model heat seal device of ZL200820164042.9 comprises upper and lower pressing plate, be embedded with Heating element in the upper and lower pressing plate respectively, press table is provided with first hole of plant test tube, top board has and corresponding second hole, press table first hole, top board and screw mandrel are fixed, the feed screw nut is limited in the nut limiting plate, and top board moves up and down under leading screw and nut mechanism drives.But this device has above-mentioned defective equally, therefore need improve existing P CR reaction tubes.

Summary of the invention

The utility model mainly be at existing PRC pipe when PCR reacts easy deformation and heat-up time long technical problem, a kind of high-speed PCR pipe is provided, the body of this high-speed PCR pipe adopts aluminium alloy structure, and thermal conductivity increases substantially, thereby has shortened experimental period greatly.

Above-mentioned technical problem of the present utility model is mainly solved by following technical proposals:

A kind of high-speed PCR pipe, the pipe lid that comprises body and match with body, described body is an aluminium alloy structure, and body comprises piped main body and the tapered bottom that is connected with main body, and described pipe lid is a split-type structural with body, and the pipe lid is made for the PP material.The PP material is a polypropylene material, and heat conductivility is better, but because hot torsion temperature lower (100 ℃) easy deformation in heat-processed repeatedly.The PP material that the utility model will be made the PCR pipe makes aluminum alloy materials into, and thermal conductivity increases greatly, and the conversion rate in the PCR experiment can improve more than 700 times even 700 times, has shortened experimental period greatly.

As preferably, the lower surface of described tapered bottom is an arc surface.The bottom of arc surface is difficult for residual reaction solution.

As preferably, to manage the upper surface of covering and be provided with an annular recesses, the Guan Gai bottom is provided with annular and gos deep into portion, and the portion of going deep into is provided with the portion of expanding to outer process.Annular recesses makes the upper transparent degree of pipe lid good, forms a form, is convenient to observe.The portion of expanding makes the cooperation of pipe lid and body tightr.

The top of described main body is provided with the outward extending annular edge of a circle level, and the outer rim on annular edge arrives the distance in body axle center less than the outer rim of pipe lid to the distance in body axle center.Annular is continued to use the tightness of covering with pipe in increasing, and reduces sample evaporation.The outer rim that the outer rim on annular edge is covered less than pipe to the distance in body axle center arrives the distance in body axle center, is convenient to manage opening of lid.

Compare with the regular-PCR plate, the PP material that the utility model will be made the PCR pipe makes aluminum alloy materials into, and thermal conductivity increases greatly, and the conversion rate in the PCR experiment can improve more than 700 times even 700 times, has shortened experimental period greatly.Significant to clinical guidance, as foreshortening to several minutes by several hours to the detection of tumor tissues and cancerous issue at ordinary times, both can strengthen the working efficiency of medical institutions, also can shorten and detect and cycle of treatment, reduce the misery that the patient stands disease.

Description of drawings

Fig. 1 is a kind of structural representation of the present utility model.

Fig. 2 is a kind of assembling synoptic diagram of the present utility model.

Label declaration: body 1, pipe cover 2, main body 11, tapered bottom 12, annular along 13, annular recesses 21 gos deep into portion 22, expands portion 23.

Embodiment

Below by embodiment, and in conjunction with the accompanying drawings, the technical solution of the utility model is described in further detail.

Employed technology in following examples unless stated otherwise, is routine techniques known to those skilled in the art; Employed plant and instrument, reagent etc., only this specification sheets specifies, is that the research of this area and technician can be by public approach acquisition.

Embodiment 1:

A kind of high-speed PCR pipe as illustrated in fig. 1 and 2 is made up of body 1 and the pipe that matches with body lid 2, and body 1 is an aluminium alloy structure, and body comprises piped main body 11 and the tapered bottom 12 that is connected with main body 11, and the lower surface of tapered bottom is an arc surface.The top of main body 11 is provided with the outward extending annular of a circle level along 13, and annular arrives the distance in body axle center less than the outer rim of pipe lid to the distance in body axle center along 13 outer rim.Pipe cover 2 and is split-type structural with body 1, and pipe covers to the PP material to be made.Pipe covers 2 upper surface and is provided with an annular recesses 21, and the Guan Gai bottom is provided with annular and gos deep into portion 22, and the portion of going deep into is provided with the portion of expanding 23 to outer process.

The PCR pipe of conventional P P material is finished PCR experiment, and the time of heating 35 needs repeatedly is to be 195 minutes in 3 hours 15 minutes, and with the high-speed PCR pipe that aluminum alloy materials is made the heat conduction time has been dwindled at least 77 times, and the time of heating 35 times is 8-15 minute.

Should be understood that this embodiment only to be used to the utility model is described and be not used in the restriction scope of the present utility model.Should be understood that in addition those skilled in the art can make various changes or modifications the utility model after the content of having read the utility model instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.

Claims

1. high-speed PCR pipe, the pipe lid (2) that comprises body (1) and match with body, it is characterized in that: described body (1) is aluminium alloy structure, body comprises piped main body (11) and the tapered bottom that is connected with main body (12), described pipe lid is a split-type structural with body, and the pipe lid is made for the PP material.

2. high-speed PCR pipe according to claim 1 is characterized in that: the lower surface of described tapered bottom is an arc surface.

3. high-speed PCR pipe according to claim 1 and 2 is characterized in that: the upper surface of pipe lid (2) is provided with an annular recesses (21), and the Guan Gai bottom is provided with annular and gos deep into portion (22), and the portion of going deep into is provided with the portion of expanding (23) to outer process.

4. high-speed PCR pipe according to claim 1 and 2, it is characterized in that: the top of described main body (11) is provided with the outward extending annular of a circle level along (13), and the outer rim on annular edge arrives the distance in body axle center greater than the distance of the outer rim of managing lid to the body axle center.

5. high-speed PCR pipe according to claim 3, it is characterized in that: the top of described main body (11) is provided with the outward extending annular of a circle level along (13), and the outer rim on annular edge arrives the distance in body axle center greater than the distance of the outer rim of managing lid to the body axle center.