CN204958896U - Draw whole blood genome DNA's device fast - Google Patents
Draw whole blood genome DNA's device fast Download PDFInfo
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- CN204958896U CN204958896U CN201520652086.6U CN201520652086U CN204958896U CN 204958896 U CN204958896 U CN 204958896U CN 201520652086 U CN201520652086 U CN 201520652086U CN 204958896 U CN204958896 U CN 204958896U
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Abstract
The utility model provides a draw whole blood genome DNA's device fast, including integration fashioned capillary collection pipe, whole blood lysis district, fluid control valve, still including with fluid control valve matched with suction device, whole blood lysis district is filled by the polypropylene silk bundle, the utility model discloses combine capillary glass tube and polypropylene silk bundle, construct can draw whole blood genome DNA's device fast ingeniously, has realized that tip blood genome DNA draws, the utility model discloses a device easy operation, efficient need not special instrument, has realized that the genome DNA who draws is suitable for a series of experiments such as the diagnostic order -checking of molecule, hybridization, isothermal increase based on the purpose of micro -fluidic chip's whole blood genome DNA " the sample advances - play as a result ".
Description
Technical field
The utility model relates to biology field, particularly relates to a kind of device of rapid extraction Whole Blood Genomic DNA.
Background technology
Nucleic acid take Nucleotide as the important biomolecule molecule of fundamental unit, is extensively present in all animals, vegetable cell, microorganism and organism.Different nucleic acid, its difference such as chemical constitution, nucleotidesequence.Different according to chemical constitution, nucleic acid can be divided into Yeast Nucleic Acid (RNA) and thymus nucleic acid (DNA).Nucleic acid is the basal component of all living things cell, also plays dominant force effect to the growth of organism, growth, breeding, the great biological phenomena such as heredity and variation.Nucleic acid has epochmaking effect in practical application, has now found that the generation of many heredopathias, tumour, the infection of virus, ray are all relevant with nucleic acid to the effect of body etc.Therefore, the understanding of nucleic acid construct, function and regulation and control is mankind in Molecular level study heredity, is evolved and the basis of medical diagnosis on disease.At present, nucleic acids research become in life science with multidisciplinaryly extensively to intersect, the study hotspot of infiltration and frontier development.Nucleic acid extraction is the prerequisite of many clinical, forensic application and genetic analysis, by extraction and the purge process of DNA or RNA, eliminates or reduces other impurity (protein, polysaccharide and the fat etc.) impact on nucleic acid amplification reaction.Traditional DNA extraction method is the solid phase extraction (Solid-PhaseExtraction, SPE) and liquid-liquid extraction method (phenol chloroform method) that in succession grow up after nineteen fifty.These method complex operation step, the extraction reagent used has toxic, limits its applying clinically.The pellosil centrifugal column method generally used at present, though the operation steps of simplifying, large fragment DNA brute force is incorporated on film, difficult wash-out, cause centrifugal DNA easy fracture when crossing post, affect downstream and test and comprise order-checking, clone, PCR, conversion, microinjection, transfection and microarray analysis.Venous blood is be commonly used to the sample that Whole Blood Genomic DNA extracts at present, and venous blood collection subcutaneous hemorrhage or local redness, local skin anaphylaxis may occur, punctures into artery and the complication such as unsuccessfully of taking a blood sample by mistake.In addition, infant's venous blood collection difficulty is larger, therefore, needs exploitation is a kind of fast, efficient, be suitable for the other detection of clinical bed the genomic extraction element of micro whole blood and method badly.Microfluidic chip technology refers to the science and technology of fluid in the small network channel of operation.Compared with the experimental technique of routine, this technology greatly reduce reagent consumption, reduce costs and produce few waste liquid simultaneously, and in microfluidic channel, heat transfer, mass transfer velocity are faster than conventional system, and reaction times or analysis time shorten all greatly.At present, many research and utilization microflow control techniques achieve the extraction of micro whole blood genomic dna, but all need special utility appliance, still adopt venous blood as detection sample, can not realize the POCT of Peripheral whole blood extracting genome DNA.
Utility model content
The utility model is the one collection peripheral blood collection solving the aforementioned problems in the prior proposition, and the device of the extraction of whole blood genomic nucleic acids and purifying and correlation method, realize quick, the high efficiency extraction of micro whole blood genomic dna.
For achieving the above object, the utility model is by the following technical solutions:
A kind of device of rapid extraction Whole Blood Genomic DNA, include the capillary collection tube of integrated molding, complete blood cell cracking zone, flow control tube, also include the suction unit matched with described flow control tube, described complete blood cell cracking zone is filled by polypropylene tows.
In order to improve further and optimize above-mentioned device, the technical measures that the utility model is taked also comprise:
Preferably, above-mentioned capillary collection tube, complete blood cell cracking zone and flow control tube are glass material making.
Preferably, above-mentioned suction unit is rubber water dropper, i.e. the water dropper of glue head dropper.
Preferably, also comprise the collection tube tube clipper that can seal capillary collection tube, and can the control tube tube clipper of fluid-encapsulated control tube.
Preferably, the long 2-6cm of above-mentioned capillary collection tube, thickness of pipe is 0.1-0.4mm, and internal diameter is 0.5-0.7mm; More preferably, above-mentioned capillary collection tube length is 4cm, and internal diameter is 0.5mm, and external diameter is 0.7mm, and thickness of pipe is 0.1mm.
Preferably, the long 1-3cm of above-mentioned flow control tube, thickness of pipe is 0.1-0.4mm, and internal diameter is 1-1.2mm; More preferably, the long 2cm of above-mentioned flow control tube, internal diameter is 1mm, and external diameter is 1.2mm, and thickness of pipe is 0.1mm.
Preferably, above-mentioned complete blood cell cracking zone thickness of pipe is 0.1-0.4mmm, and volume is 100-130 μ l; More preferably, complete blood cell cracking zone is the spheroidal (calculating with outer wall) of diameter 6mm, and thickness of pipe is 0.2mm.
Polypropylene tows has nontoxic, tasteless, heat-resisting, chemical property good, do not absorb water, and the organic solvents such as common acid, alkali the feature such as to work hardly to it.Polypropylene tows has multi-pore structure and can be used as filter material, is widely used as filter tip in cigarette, and, the material such as nicotine (be commonly called as Nicotine), carbon monoxide coal-tar middle oil to flue gas carries out adsorption filtration.The utility model, by itself and the ingenious combination of glass capillary, utilizes special structure to make device of the present utility model can meet the demand of rapid extraction Whole Blood Genomic DNA in the other detection of bed.
The utility model adopts technique scheme, compared with prior art, has following technique effect:
The utility model is in conjunction with glass capillary and polypropylene tows, constructing dexterously can the device of rapid extraction Whole Blood Genomic DNA, achieve peripheral blood extracting genome DNA, device of the present utility model is simple to operate, efficiency is high, without the need to specific apparatus, achieve the object of the Whole Blood Genomic DNA " sample enter-result go out " based on micro-fluidic chip, the genomic dna extracted is suitable for the series of experiments such as order-checking, hybridization, isothermal duplication of molecular diagnosis.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the genomic dna result figure that agarose gel electrophoresis analysis is extracted.
Embodiment
Below in conjunction with accompanying drawing, the technical solution of the utility model is described in further detail.
Fig. 1 is the structural representation of the device of a kind of rapid extraction Whole Blood Genomic DNA of the present utility model, and Reference numeral is wherein capillary collection tube 1, complete blood cell cracking zone 2, flow control tube 3, suction unit 4, collection tube tube clipper 5, control tube tube clipper 6.
The utility model provides a kind of device of rapid extraction Whole Blood Genomic DNA, include the capillary collection tube 1 of integrated molding, complete blood cell cracking zone 2, flow control tube 3, also include the suction unit 4 matched with described flow control tube 3, described complete blood cell cracking zone 2 is filled by polypropylene tows.
Device kapillary of the present utility model adopts glass material, draws pin device to be made through kapillary.Complete blood cell cracking zone 2 fill polypropylene tows have nontoxic, tasteless, heat-resisting, chemical property is good, does not absorb water, the organic solvents such as common acid, alkali work hardly to it.Polypropylene tows has multi-pore structure and can be used as filter material, is widely used as filter tip in cigarette, and, the material such as nicotine (be commonly called as Nicotine), carbon monoxide coal-tar middle oil to flue gas carries out adsorption filtration.The utility model adopts polypropylene tows to extract peripheral blood genomic dna in conjunction with kapillary, and polypropylene tows surface has multi-pore structure, the feature of its high hole density and porosity, under certain conditions can efficient adsorption DNA.
Carry out detailed and concrete introduction below by specific embodiment to the utility model, to make better to understand the utility model, but following embodiment does not limit the utility model scope.
Embodiment one
Operator blood sampling person utilizes capillary collection tube 1 to thrust subjects skin and gathers peripheral blood 5 μ l, uses suction unit 4 all to suck in complete blood cell cracking zone 2 by the peripheral blood of collection; Utilize suction unit 4 to be sucked in complete blood cell cracking zone 2 by 10 μ l lysates and carry out lysis, use collection tube tube clipper 5, control tube tube clipper 6 seals capillary collection tube 1 respectively and flow control tube 3,56 DEG C continues 10 minutes; Then remove tube clipper 5 and tube clipper 6, utilize suction unit 4 to suck in complete blood cell cracking zone 2 by 15 μ l dehydrated alcohols; Utilize suction unit 4 that 75% ethanol of 100 μ l is sucked complete blood cell cracking zone 2 and carry out rinsing, rinse cycle carries out 2 times repeatedly; Finally, utilize suction unit 4 that 25 μ l deionized waters are sucked complete blood cell cracking zones 2 and DNA wash-out is collected elutriant; The Whole Blood Genomic DNA obtained is utilized to carry out next step mensuration.
Lysate pH is 8, and component wherein and concentration are: 10mMTris-Cl, 15mMNaCl, 10mMEDTA, 0.4%SDS, 200 μ g/ml Proteinase Ks.
Embodiment two
Operator blood sampling person utilizes capillary collection tube 1 to thrust subjects skin and gathers peripheral blood 10 μ l, uses suction unit 4 all to suck in complete blood cell cracking zone 2 by the peripheral blood of collection; Utilize suction unit 4 to be sucked in complete blood cell cracking zone 2 by 20 μ l lysates and carry out lysis, use collection tube tube clipper 5, control tube tube clipper 6 seal capillary collection tube 1 and flow control tube 3 respectively, 56 DEG C continue 10 minutes, lysate pH is 8, component wherein and concentration are: 10mMTris-Cl, 15mMNaCl, 10mMEDTA, 0.4%SDS, 200 μ g/ml Proteinase Ks; Then; Then remove tube clipper 5 and tube clipper 6, utilize suction unit 4 to suck in complete blood cell cracking zone 2 by 30 μ l dehydrated alcohols; Utilize suction unit 4 that 75% ethanol of 110 μ l is sucked complete blood cell cracking zone 2 and carry out rinsing, rinse cycle carries out 3 times repeatedly; Finally, utilize suction unit 4 that 50 μ l deionized waters are sucked complete blood cell cracking zone 2 by DNA wash-out, obtain Whole Blood Genomic DNA; The Whole Blood Genomic DNA obtained is utilized to carry out next step mensuration.
Embodiment three
Operator blood sampling person utilizes capillary collection tube 1 to thrust subjects skin and gathers peripheral blood 20 μ l, uses suction unit 4 all to suck in complete blood cell cracking zone 2 by the peripheral blood of collection; Utilize suction unit 4 to be sucked in complete blood cell cracking zone 2 by 40 μ l lysates and carry out lysis, use collection tube tube clipper 5, control tube tube clipper 6 seal capillary collection tube 1 and flow control tube 3 respectively, continue 56 DEG C 10 minutes, lysate pH is 8, component wherein and concentration are: 10mMTris-Cl, 15mMNaCl, 10mMEDTA, 0.4%SDS, 200 μ g/ml Proteinase Ks; Then remove tube clipper 5 and tube clipper 6, utilize suction unit 4 to suck in complete blood cell cracking zone 2 by 60 μ l dehydrated alcohols; Utilize suction unit 4 that 75% ethanol of 110 μ l is sucked complete blood cell cracking zone 2 and carry out rinsing, rinse cycle carries out 5 times repeatedly; Finally, utilize suction unit 4 that 100 μ l deionized waters are sucked complete blood cell cracking zone 2 by DNA wash-out, obtain Whole Blood Genomic DNA; The Whole Blood Genomic DNA obtained is utilized to carry out next step mensuration.
The genomic dna utilizing agarose gel electrophoresis to analyze above-described embodiment extraction the results are shown in Figure 2.M swimming lane is λ DNA/Hind III electrophoresis result; 1 swimming lane is the electrophoresis result (50kb) of λ DNA; 2,3,4 swimming lanes correspond respectively to the DNA electrophoresis result that embodiment one, embodiment two and embodiment three peripheral blood extract.The single electrophoretic band that the genomic dna utilizing apparatus and method of the present utility model to extract is 30-50kb, proves the integrity of the DNA that the utility model device extracts.
The utility model is in conjunction with glass capillary and polypropylene tows, constructing dexterously can the device of rapid extraction Whole Blood Genomic DNA, achieve peripheral blood extracting genome DNA, device of the present utility model is simple to operate, efficiency is high, without the need to specific apparatus, achieve the object of the Whole Blood Genomic DNA " sample enter-result go out " based on micro-fluidic chip, the genomic dna extracted is suitable for the series of experiments such as order-checking, hybridization, isothermal duplication of molecular diagnosis.
Be described in detail specific embodiment of the utility model above, but it is as example, the utility model is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and substituting also all among category of the present utility model.Therefore, not departing from the equalization conversion and amendment done under spirit and scope of the present utility model, all should be encompassed in scope of the present utility model.
Claims (10)
1. the device of a rapid extraction Whole Blood Genomic DNA, it is characterized in that, include the capillary collection tube (1) of integrated molding, complete blood cell cracking zone (2), flow control tube (3), also include the suction unit (4) matched with described flow control tube (3), described complete blood cell cracking zone (2) is filled by polypropylene tows.
2. device according to claim 1, is characterized in that, described capillary collection tube (1), complete blood cell cracking zone (2) and flow control tube (3) are glass material and make.
3. device according to claim 1, is characterized in that, described suction unit (4) is rubber water dropper.
4. device according to claim 1, it is characterized in that, also comprise the collection tube tube clipper (5) that can seal capillary collection tube (1), and can the control tube tube clipper (6) of fluid-encapsulated control tube (3).
5. device according to claim 1 and 2, is characterized in that, described capillary collection tube (1) long 2-6cm, and thickness of pipe is 0.1-0.4mm, and internal diameter is 0.5-0.7mm.
6. device according to claim 1 and 2, is characterized in that, described flow control tube (3) long 1-3cm, and thickness of pipe is 0.1-0.4mm, and internal diameter is 1-1.2mm.
7. device according to claim 1 and 2, is characterized in that, described complete blood cell cracking zone (2) is spheroidal, and thickness of pipe is 0.1-0.4mmm, and volume is 100-130 μ l.
8. device according to claim 5, is characterized in that, described capillary collection tube (1) long 4cm, and internal diameter is 0.5mm, and external diameter is 0.7mm.
9. device according to claim 6, is characterized in that, described flow control tube (3) long 2cm, and internal diameter is 1mm, and external diameter is 1.2mm.
10. device according to claim 7, is characterized in that, described complete blood cell cracking zone (2) is spheroidal, and thickness of pipe is 0.2mmm, and volume is 110 μ l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520652086.6U CN204958896U (en) | 2015-08-26 | 2015-08-26 | Draw whole blood genome DNA's device fast |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520652086.6U CN204958896U (en) | 2015-08-26 | 2015-08-26 | Draw whole blood genome DNA's device fast |
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| CN204958896U true CN204958896U (en) | 2016-01-13 |
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| CN201520652086.6U Active CN204958896U (en) | 2015-08-26 | 2015-08-26 | Draw whole blood genome DNA's device fast |
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