CN215440387U - Quick extraction element of nucleic acid - Google Patents
Quick extraction element of nucleic acid Download PDFInfo
- Publication number
- CN215440387U CN215440387U CN202120876173.5U CN202120876173U CN215440387U CN 215440387 U CN215440387 U CN 215440387U CN 202120876173 U CN202120876173 U CN 202120876173U CN 215440387 U CN215440387 U CN 215440387U
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- push rod
- nucleic acid
- hollow tube
- rotary push
- rotary
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Abstract
The utility model discloses a rapid nucleic acid extraction device, which belongs to the field of development of biological product inspection and quarantine tools and specifically comprises the following components: the device comprises a rotary push rod, a hollow tube, a rubber sleeve, a filter element and a collecting bottle device, wherein the rotary push rod is combined by a manual rotary rod and a propeller, the rotary push rod is embedded in the hollow tube, the hollow tube is shaped like a syringe for injection, the filter element is embedded in the tail end of the hollow tube, the tail end of the hollow tube is externally connected with a detachable collecting bottle, and the collecting bottle is used for collecting nucleic acid; the whole device is made of Polycarbonate (PC) materials, and is light and easy to carry. The nucleic acid extraction device that this patent provided, it is simple portable, can accomplish in 10 minutes from organizing the sample to handle the purpose of extracting fast and preliminary purification separation of nucleic acid, be applicable to the quick preliminary screening work of a large amount of samples in the first line of extensive clinical examination, improve work efficiency.
Description
Technical Field
The utility model relates to the field of biological product inspection and quarantine tools, in particular to a rapid primary screening device for a large number of samples in extensive clinical first-line detection.
Background
Nucleic acid extraction is one of the most basic steps in the fields of clinical inspection and quarantine, molecular biology scientific research and the like at present. The existing traditional extraction method at home and abroad relates to relatively complicated steps such as tissue and cell wall crushing, cell cracking, impurity (mainly protein) removal, nucleic acid precipitation and the like. Generally, the longer the operation, the more damage to the nucleic acid is inevitable.
In addition, for some clinical first-line tests, rapid extraction and separation of a large number of samples are required, and the conventional method is not applicable. There are also relatively rapid means of nucleic acid extraction using kits. However, the cost of the kit is high, most of the kits still need to be operated in a laboratory, and the kit is not very convenient for field investigation. The method of the kit is relatively long in time consumption, and usually needs more than 20-30 min.
Therefore, the utility model designs the rapid nucleic acid extraction device from the aspect of improving the working efficiency, and the device selects light and portable artificial materials, is non-toxic, has high stability and has high efficiency in sample collection.
SUMMERY OF THE UTILITY MODEL
The utility model provides a rapid extraction device aiming at improving the efficiency of nucleic acid extraction in the fields of clinical inspection and quarantine, molecular biology scientific research and the like. The device provides guarantee for carrying out quick primary screening work of a large number of samples of nucleic acid more efficiently. In order to solve the problems in the prior art, the utility model provides a rapid nucleic acid extraction device which comprises a rotary push rod, a hollow tube, a rubber sleeve, a filter element and a collection bottle, wherein the rotary push rod is an integrated assembly of a manual rotary rod and a push rod, the upper section of the rotary push rod is an injector push rod, and the top end of the push rod is a knob; the middle section is a disposable spiral knife, and the length of the disposable spiral knife is 4-8 cm; the bottom of the rotary push rod is a part connected with the filter element, a rubber sleeve is arranged at the position contacting with the filter element, the hollow tube is sleeved outside the rotary push rod, and the whole rotary push rod and the hollow tube are externally shaped like a syringe for injection; the filter core is arranged below the rubber sleeve, the filter core is a sand core structure embedded at the bottom in the hollow tube, the outer bottom of the hollow tube below the filter core is a tip similar to a syringe injector, and the detachable collecting bottle is connected below the hollow tube.
As a further improvement of the utility model, the hollow tube is made into a specification with the outer diameter phi of 9-14 mm and the inner diameter phi of 7-12 mm, and is matched with the rotary push rod, and a negative pressure is formed between the hollow tube and the rotary push rod by a rubber sleeve at the bottom end of the rotary push rod.
As a further improvement of the utility model, the rotating push rod has a diameter phi of 5-10 mm.
As a further improvement of the utility model, the length of the propelling rod is 5-10 mm.
As a further improvement of the utility model, the knob is a butterfly knob.
As a further improvement of the utility model, the bottom of the rotary push rod is 5-10 mm long.
As a further improvement of the utility model, the thickness of the rubber sleeve is 7-12 mm.
As a further improvement of the utility model, the aperture of the sand core is 0.45-0.80 μm.
As a further improvement of the utility model, the volume of the collecting bottle is 5-10 mL.
The utility model has the beneficial effects that:
the nucleic acid extraction device provided by the utility model is simple and portable, can finish the purposes of rapid extraction and preliminary purification and separation of nucleic acid from tissue sample treatment within 10 minutes, is suitable for rapid preliminary screening work of a large number of samples in extensive clinical first-line detection, and improves the working efficiency.
Drawings
FIG. 1 is a schematic structural diagram of a rapid nucleic acid extraction apparatus according to the present invention.
Detailed Description
The utility model is further described below with reference to the accompanying drawings.
Referring to fig. 1, the nucleic acid rapid extraction device of the present invention comprises the following components: the device comprises a rotary push rod 1, a hollow pipe 2, a rubber sleeve 3, a filter element 4 and a collecting bottle 5.
The rotary push rod 1 is an integrated combined fitting of a manual rotary rod and a push rod and can be made into a thick material
Specification; the upper section of the syringe is provided with a syringe push rod, the length of the push rod is 5-10 mm, and the top end of the push rod is provided with a butterfly knob; the middle section is a disposable spiral knife, and the length of the disposable spiral knife is 4-8 cm; the bottom of the rotary push rod 1 is a part connected with the filter element, the bottom is 5-10 mm long, the rubber sleeve 3 is arranged at the position contacting with the filter element 4, and the thickness of the rubber sleeve 3 is 7-12 mm.
The hollow tube 2 is sleeved outside the rotary push rod, and the whole rotary push rod and the hollow tube are shaped like a syringe for injection; the hollow tube can be made into an external diameter
Inner diameter
The specification is matched with the rotary push rod, and negative pressure is formed between the rotary push rod and the rotary push rod by a rubber sleeve at the bottom end of the rotary push rod. The filter core 4 is arranged below the rubber sleeve, the filter core 4 is a sand core structure embedded at the bottom in the hollow pipe, and the aperture of the sand core is 0.45-0.80 mu m. The bottom of the outer part of the hollow tube 2 below the filter element 4 is a tip similar to a syringe injector, the lower part of the hollow tube is connected with a detachable collecting bottle 5, and the volume of the collecting bottle 5 is 5-10 mL.
The whole assembly mode of the nucleic acid rapid extraction device is shown in figure 1, namely, a rotary push rod 1 and a rubber sleeve 3 wrapped at the bottom of the rotary push rod are inserted into a hollow tube 2 with a filter element 4, and a collecting bottle 5 is connected below the rotary push rod to collect extracted nucleic acid. When in use, the nested rotary push rod 1 is pulled out, and a sample (animal and plant tissues or bacterial cell suspension) and a cell lysate (which can be replaced by a common detergent in field investigation) are sequentially added into the hollow tube 2; inserting the pulled rotary push rod 1, shaking the whole device up and down by 3-5 times, and rotating a butterfly knob at the top of the rotary push rod 1 for about 20-30 circles; after standing for 3-5 seconds, pulling out the rotary push rod 1 again, and adding 7.5mol/L ammonium acetate solution (or 5mol/L sodium chloride solution) with the volume being about 1/4 of the sample; the rotary push rod 1 is inserted again, the whole device shakes 5-10 times up and down, after standing for 10 seconds, the rotary push rod is pushed to the contact bottom, liquid enters the collecting bottle 5 through the filter element 4, the primary separated nucleic acid solution can be collected, and follow-up detection can be directly carried out after dilution by adding ultrapure water.
The foregoing is a more detailed description of the utility model in connection with specific preferred embodiments and it is not intended that the utility model be limited to these specific details. For those skilled in the art to which the utility model pertains, several simple deductions or substitutions can be made without departing from the spirit of the utility model, and all shall be considered as belonging to the protection scope of the utility model.
Claims (9)
1. A nucleic acid rapid extraction device is characterized in that: the device comprises a rotary push rod (1), a hollow tube (2), a rubber sleeve (3), a filter core (4) and a collecting bottle (5), wherein the rotary push rod (1) is an integrated assembly of a manual rotary rod and a push rod, the upper section of the rotary push rod (1) is an injector push rod, and the top end of the push rod is a knob; the middle section is a disposable spiral knife, and the length of the disposable spiral knife is 4-8 cm; the bottom of the rotary push rod (1) is a part connected with the filter element, a rubber sleeve (3) is arranged at a position contacting with the filter element (4), the hollow tube (2) is sleeved outside the rotary push rod, and the whole rotary push rod and the hollow tube are externally shaped like a syringe for injection; the filter core (4) is arranged below the rubber sleeve, the filter core (4) is a sand core structure embedded at the bottom in the hollow tube, the outer bottom of the hollow tube (2) below the filter core (4) is a tip similar to a syringe injector, and the detachable collecting bottle (5) is connected below.
2. The device for rapidly extracting nucleic acid according to claim 1, wherein: the hollow tube is made into specifications of ¢ 9-14 mm in outer diameter and ¢ 7-12 mm in inner diameter, is matched with the rotary push rod, and forms negative pressure between the hollow tube and the rotary push rod by virtue of a rubber sleeve at the bottom end of the rotary push rod.
3. The device for rapidly extracting nucleic acid according to claim 1, wherein: the rotating push rod (1) is ¢ 5-10 mm thick.
4. The device for rapidly extracting nucleic acid according to claim 1, wherein: the length of the pushing rod is 5-10 mm.
5. The device for rapidly extracting nucleic acid according to claim 1, wherein: the knob is a butterfly knob.
6. The device for rapidly extracting nucleic acid according to claim 1, wherein: the bottom of the rotary push rod (1) is 5-10 mm long.
7. The device for rapidly extracting nucleic acid according to claim 1, wherein: the thickness of the rubber sleeve (3) is 7-12 mm.
8. The device for rapidly extracting nucleic acid according to claim 1, wherein: the aperture of the sand core is 0.45-0.80 mu m.
9. The device for rapidly extracting nucleic acid according to claim 1, wherein: the volume of the collecting bottle (5) is 5-10 mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120876173.5U CN215440387U (en) | 2021-04-26 | 2021-04-26 | Quick extraction element of nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120876173.5U CN215440387U (en) | 2021-04-26 | 2021-04-26 | Quick extraction element of nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215440387U true CN215440387U (en) | 2022-01-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120876173.5U Active CN215440387U (en) | 2021-04-26 | 2021-04-26 | Quick extraction element of nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215440387U (en) |
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2021
- 2021-04-26 CN CN202120876173.5U patent/CN215440387U/en active Active
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