CN205803502U - A kind of tumor stem cell culture dish - Google Patents
A kind of tumor stem cell culture dish Download PDFInfo
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- CN205803502U CN205803502U CN201620756984.0U CN201620756984U CN205803502U CN 205803502 U CN205803502 U CN 205803502U CN 201620756984 U CN201620756984 U CN 201620756984U CN 205803502 U CN205803502 U CN 205803502U
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- stem cell
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- culture dish
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Abstract
This utility model relates to a kind of tumor stem cell culture dish, including ware body, ware lid, it is characterised in that: ware body bottom center is fixed with a circular cultivation region, and circular cultivation region is provided with cylindrical cell culture well not of uniform size.This utility model designs according to the feature of tumor stem cell incubation so that can complete the cultivation of tumor stem cell in a culture dish, has saved test consumable and cell culture medium, simplifies the operating procedure that tumor stem cell is cultivated.
Description
Technical field
This utility model relates to a kind of stem cell culture dish being applied to medical science or biological study field, and specially one
Plant tumor stem cell culture dish.
Background technology
Recent study finds that there is a group in tumor tissues has the cell of normal stem cell feature, and they have oneself
The molecule of specifically expressing, this group of cells are named as tumor stem cell.Tumor stem cell is the root of tumor recurrence and transfer, because of
The research of this its functional mechanism has profound significance to the clinical prevention of tumor disease.
The separation of tumor stem cell at present mainly uses suspension culture separating method, with the separation and Culture of lung cancer tumor stem cell
As a example by: the lung cancer tumor cell line A549 cell of trophophase of taking the logarithm, it is inoculated in ultralow absorption culture dish by certain density, in
37 DEG C of saturated humidities, 5% CO2Incubator in cultivate after 2～3 weeks, form tumor spheres；Utilize Pasteur's pipe in microscope
In the lower cultivation unit (i.e. Tissue Culture Plate) that single tumor spheres is transferred to suitable size, adding pancreatin substitute will
Cell ball digestion, for unicellular, add culture medium amplification cultivation afterwards and re-forms stem cell ball；Along with the amplification of cell number, will
Tumor spheres is progressively transferred to bigger specification and cultivates in unit, and after digesting, cell number is more than 105Can frozen or transfer
Continue to cultivate to general ultra low absorption culture dish.This process, needs the cell using Tissue Culture Dish and plurality of specifications to cultivate
Plate, operates complicated, repeatedly changes Tissue Culture Plate and too increases the risk of cell contamination.For the cell that cell hole specification is consistent
Culture plate, disposable operation can not exhaust, but Tissue Culture Plate is aseptic medical disposable material, in order to ensure that cell is trained
Support aseptic, unworn culture hole the most just not in use by, thus cause test consumptive material waste.
Summary of the invention
For the problems referred to above, the invention provides a kind of tumor stem cell culture dish, simplify what tumor stem cell was cultivated
Operating procedure, shortens the time of experimental implementation, reduces the risk of cell contamination, saves test consumable, reduces cost.
This utility model is achieved through the following technical solutions:
A kind of tumor stem cell culture dish, including ware body and ware lid, is fixed with a circular cultivation region bottom described ware body,
The surrounding of described circular cultivation region is evenly distributed with the cylindrical cell culture well of several causes not of uniform size.
Further, a diameter of 6mm ~ 10mm of described cell culture well, high unity is 10mm to this utility model.
As one of preferred version of the present utility model, described circular cultivation region is positioned at ware body central authorities.
As one of preferred version of the present utility model, described circular cultivation region is tangent with the periphery certain point of ware body.
The beneficial effects of the utility model are: this utility model is fixed with a circular cultivation region and not bottom culture dish
The culture hole of same specification size, so in the incubation of tumor stem cell, is first placed in circular cultivation region by tumor cell line
Cultivate, after stem cell ball to be formed, utilize Pasteur's pipe that individual cells ball is transferred under microscope the cultivation list of suitable size
In unit, first by discrete for stem cell ball for individual cells amplification cultivation again, along with the increase of cell number, stem cell ball is moved successively
To bigger culture hole, until obtaining the stem line that cell number is abundant.So culture dish is closed two with Tissue Culture Plate
Being one, whole process need not change Tissue Culture Plate, with one piece of Tissue Culture Dish, can complete the institute that tumor stem cell is cultivated
In steps, easy and simple to handle, save consumptive material, reduce cell contamination risk.
Accompanying drawing explanation
Fig. 1 this utility model side view.
The ware body front view of one of Fig. 2 this utility model preferred version.
The ware body front view of the two of Fig. 3 this utility model preferred version.
Detailed description of the invention
The present invention will be described with specific embodiment below in conjunction with the accompanying drawings, for embodiment be only to this utility model
Make generality to illustrate, contribute to being better understood from this utility model, but be not limiting upon scope of the present utility model.
Such as Fig. 1-3, this utility model one tumor stem cell culture dish, including ware body 1, ware lid 2, bottom described ware body 1
Being fixed with a circular cultivation region 3, the surrounding of described circular cultivation region 3 is evenly distributed with the cylinder of several causes not of uniform size
Cell culture well 4.
Choose one of preferred version of the present utility model: described cell culture well 4 uses 3 kinds of specifications, is followed successively by 6 × 10,8
× 10,10 × 10(diameter × height, unit: mm).
Such as Fig. 2, for one of preferred version of the present utility model: described circular cultivation region 3 is positioned at ware body 1 central authorities, every kind of rule
The cell of lattice is cultivated culture hole and is respectively arranged with 5.
Such as Fig. 3, for one of preferred version of the present utility model: described circular cultivation region 3 and ware body 1 periphery certain point phase
Cutting, the cell culture well of a diameter of 6mm has 5, and the cell culture well of remaining two kinds of specification is respectively arranged with 4.
As a example by the cultivation of lung cancer tumor stem cell, first A594 cell is placed in circular cultivation region 3 and cultivates, to be formed dry thin
After born of the same parents' ball, Pasteur's pipe is utilized to be transferred in culture hole 4 by individual cells ball under microscope, first by discrete for stem cell ball for slender
Born of the same parents' amplification cultivation again, along with the increase of cell number, moves in bigger culture hole 4 successively by stem cell ball, until obtaining thin
The stem line that born of the same parents' number is abundant.This process need not change Tissue Culture Plate, the most convenient to operate, has saved again test simultaneously
Consumptive material and cell culture medium.
Claims (4)
1. a tumor stem cell culture dish, including ware body (1), ware lid (2), it is characterised in that: described ware body (1) bottom is fixing
Having a circular cultivation region (3), the surrounding of described circular cultivation region (3) is evenly distributed with the cylinder of several causes not of uniform size
Cell culture well (4).
A kind of tumor stem cell culture dish the most according to claim 1, it is characterised in that:
A diameter of 6mm ~ 10mm of described cell culture well (4), high unity is 10 mm.
A kind of tumor stem cell culture dish the most according to claim 1, it is characterised in that:
Described circular cultivation region (3) is positioned at ware body (1) central authorities.
A kind of tumor stem cell culture dish the most according to claim 1, it is characterised in that:
Described circular cultivation region (3) is tangent with the certain point of the periphery of ware body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620756984.0U CN205803502U (en) | 2016-07-19 | 2016-07-19 | A kind of tumor stem cell culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620756984.0U CN205803502U (en) | 2016-07-19 | 2016-07-19 | A kind of tumor stem cell culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205803502U true CN205803502U (en) | 2016-12-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620756984.0U Active CN205803502U (en) | 2016-07-19 | 2016-07-19 | A kind of tumor stem cell culture dish |
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| CN (1) | CN205803502U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107858287A (en) * | 2017-12-23 | 2018-03-30 | 淮北智淮科技有限公司 | A kind of stem cell detection cell culture apparatus |
-
2016
- 2016-07-19 CN CN201620756984.0U patent/CN205803502U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107858287A (en) * | 2017-12-23 | 2018-03-30 | 淮北智淮科技有限公司 | A kind of stem cell detection cell culture apparatus |
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| GR01 | Patent grant |