CN204958934U - A mancarried device that is used for capillary isothermal to amplify and detect - Google Patents
A mancarried device that is used for capillary isothermal to amplify and detect Download PDFInfo
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- CN204958934U CN204958934U CN201520617507.1U CN201520617507U CN204958934U CN 204958934 U CN204958934 U CN 204958934U CN 201520617507 U CN201520617507 U CN 201520617507U CN 204958934 U CN204958934 U CN 204958934U
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Abstract
The utility model discloses a mancarried device that is used for capillary isothermal to amplify and detect, including casing and the LAMP that is located the casing amplify temp. Control module and fluorescence detection module, LAMP amplifys temp. Control module and includes the temperature -control circuit board and be located on the temperature -control circuit board and the outer temperature controller of outstanding casing, is equipped with the window on the casing, is located window department and is equipped with the aluminium groove piece that is used for placing the capillary, the fluorescence detection 0 of fluorescence detection module above be located LED lamp array that aluminium groove piece below up set gradually under from, launch the light filter and be located the aluminium groove piece. This a mancarried device that is used for capillary isothermal to amplify and detect amplifys temp. Control module and fluorescence detection module integration in the casing with LAMP, controls amplify temp. Control module's temperature of LAMP through the temperature -control circuit board, must encircle to mediate the isothermal and amplify and fluorescence detection in the casing of little volume, and here utility model is used for the check out test set field.
Description
Technical field
The utility model relates to medical instruments field, particularly relates to a kind of mancarried device for kapillary isothermal duplication and detection.
Background technology
Loop-mediated isothermal amplification technique can under isothermal (60 ~ 65 DEG C) condition, for the zone design of six on target gene four primers, strand displacement type archaeal dna polymerase is utilized to carry out amplified reaction under constant temperature, can realize the amplification of 109 ~ 1010 times in 15 ~ 60 minutes, be the gene amplification method of a kind of " easy, quick, accurate, at a low price ".
This amplification technique, compared with Standard PCR, does not need the processes such as the thermally denature of template, temperature cycle, electrophoresis and ultraviolet visualization.Ring mediated isothermal amplification method is a kind of brand-new nucleic acid amplification method, has feature that is simple, quick, high specificity.This technology can match in excellence or beauty and even be better than round pcr in the indexs such as sensitivity, specificity and sensing range, and do not rely on any special plant and instrument and realize on-the-spot high-throughput rapid detection, testing cost is far below quantitative fluorescent PCR.Low in the requirement of application stage to instrument, a simple thermostat just energy realization response, result detects also very simple, direct visual inspection white precipitate or green fluorescence, needing to carry out gel electrophoresis observations unlike regular-PCR method, is a kind of method of applicable scene, basic unit's rapid detection.
Carry out ring mediated isothermal amplification at present and utilize PCR instrument device or quantitative real time PCR Instrument more, but this equipment price is expensive, volume is comparatively large, is not suitable for field quick detection.
Utility model content
For solving the problem, the utility model provide a kind of can isothermal heating and the less mancarried device for kapillary isothermal duplication and detection of volume.
The utility model solves the technical scheme that its technical problem adopts:
A kind of mancarried device for kapillary isothermal duplication and detection, comprise housing and the LAMP amplification temperature control module and the fluoroscopic examination module that are positioned at housing, the LAMP temperature control module that increases comprises temperature control circuit board and to be positioned in temperature control circuit board and temperature regulator outside outstanding housing, housing is provided with window, be positioned at window place and be provided with aluminium geosynclinal block for placing kapillary, fluoroscopic examination module comprise be positioned at set gradually from the bottom up below aluminium geosynclinal block LED array, launch spectral filter and be positioned at the transmission filter above aluminium geosynclinal block.
Be further used as the improvement of technical solutions of the utility model, aluminium geosynclinal block is positioned at the window's position and protrudes from the upper surface of housing, and aluminium geosynclinal block is provided with some pipe standing grooves arranged in parallel.
Be further used as the improvement of technical solutions of the utility model, LAMP amplification temperature control module also comprises the ito glass being positioned at aluminium geosynclinal block and launching between spectral filter, and is positioned at the platinum heat posted sheet of each pipe standing groove.
Be further used as the improvement of technical solutions of the utility model, temperature control circuit board is provided with the temperature indicating device protruding from housing upper surface.
The beneficial effects of the utility model: LAMP is increased temperature control module and fluoroscopic examination module integration in housing by this mancarried device for kapillary isothermal duplication and detection, by the temperature of temperature control circuit board control LAMP amplification temperature control module, guarantee to carry out ring mediated isothermal amplification and fluoroscopic examination in the housing of small volume.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is described in further detail:
Fig. 1 is the utility model embodiment one-piece construction exploded perspective view;
Fig. 2 is the utility model embodiment one-piece construction schematic perspective view;
Fig. 3 is the utility model embodiment one-piece construction vertical view.
Embodiment
With reference to Fig. 1 ~ Fig. 3, the utility model is a kind of mancarried device for kapillary isothermal duplication and detection, comprise housing 1 and the LAMP amplification temperature control module and the fluoroscopic examination module that are positioned at housing 1, the LAMP temperature control module that increases comprises temperature control circuit board 8 and to be positioned in temperature control circuit board 8 and temperature regulator 10 outside outstanding housing 1, housing 1 is provided with window, be positioned at window place and be provided with aluminium geosynclinal block 3 for placing kapillary 9, fluoroscopic examination module comprises the LED array 6 being positioned at and setting gradually from the bottom up below aluminium geosynclinal block 3, the transmission filter 2 launched spectral filter 5 and be positioned at above aluminium geosynclinal block 3.
LAMP is increased temperature control module and fluoroscopic examination module integration in housing 1 by this mancarried device for kapillary isothermal duplication and detection, by the temperature of temperature control circuit board control LAMP amplification temperature control module, guarantee to carry out ring mediated isothermal amplification and fluoroscopic examination in the housing 1 of small volume.
As the improvement of technical solutions of the utility model, aluminium geosynclinal block 3 is positioned at the window's position and protrudes from the upper surface of housing 1, and aluminium geosynclinal block 3 is provided with some pipe standing grooves arranged in parallel.
As the improvement of technical solutions of the utility model, LAMP amplification temperature control module also comprises the ito glass 4 being positioned at aluminium geosynclinal block 3 and launching between spectral filter 5, and is positioned at the platinum heat posted sheet 7 of each pipe standing groove.
As the utility model preferred embodiment, temperature control circuit board 8 is provided with the temperature indicating device protruding from housing 1 upper surface.
This device in use, after LAMP reaction solution is mixed with template to be measured and mineral oil add in kapillary 9 respectively, each kapillary 9 is positioned over successively in the pipe standing groove in this device on aluminium block groove 3, regulates temperature regulator 10, ito glass 4 and aluminium block are heated realize LAMP to react and increase.
Amplification temperature monitoring is realized by platinum hot paste sheet 7, and LAMP reaction, after about 60 minutes, opens LED array 6, and make light successively through transmitting spectral filter 5, TIO glass 4, pipe standing groove, transmission filter 2, recycling photographic camera or CCD take fluorescent signal.
Certainly, the invention is not limited to above-mentioned embodiment, those of ordinary skill in the art also can make equivalent variations or replacement under the prerequisite without prejudice to the utility model spirit, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (4)
1. the mancarried device for kapillary isothermal duplication and detection, it is characterized in that: the LAMP comprising housing (1) and be positioned at described housing (1) increases temperature control module and fluoroscopic examination module, described LAMP amplification temperature control module comprises temperature control circuit board (8) and is positioned at described temperature control circuit board (8) to be gone up and gives prominence to housing (1) temperature regulator outward (10), described housing (1) is provided with window, be positioned at described window place and be provided with aluminium geosynclinal block (3) for placing kapillary (9), described fluoroscopic examination module comprises the LED array (6) being positioned at aluminium geosynclinal block (3) below and setting gradually from the bottom up, the transmission filter (2) launched spectral filter (5) and be positioned at above aluminium geosynclinal block (3).
2. the mancarried device for kapillary isothermal duplication and detection according to claim 1, it is characterized in that: described aluminium geosynclinal block (3) is positioned at the window's position and protrudes from the upper surface of housing (1), described aluminium geosynclinal block (3) is provided with some pipe standing grooves arranged in parallel.
3. the mancarried device for kapillary isothermal duplication and detection according to claim 2, it is characterized in that: described LAMP amplification temperature control module also comprises the ito glass (4) being positioned at aluminium geosynclinal block (3) and launching between spectral filter (5), and is positioned at the platinum heat posted sheet (7) of each pipe standing groove.
4., according to the mancarried device for kapillary isothermal duplication and detection in claims 1 to 3 described in any one, it is characterized in that: described temperature control circuit board (8) is provided with the temperature indicating device protruding from housing (1) upper surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520617507.1U CN204958934U (en) | 2015-08-17 | 2015-08-17 | A mancarried device that is used for capillary isothermal to amplify and detect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520617507.1U CN204958934U (en) | 2015-08-17 | 2015-08-17 | A mancarried device that is used for capillary isothermal to amplify and detect |
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|---|---|
| CN204958934U true CN204958934U (en) | 2016-01-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520617507.1U Active CN204958934U (en) | 2015-08-17 | 2015-08-17 | A mancarried device that is used for capillary isothermal to amplify and detect |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109307653A (en) * | 2017-07-27 | 2019-02-05 | 国家纳米科学中心 | Capillary detection platform based on ring mediated isothermal nucleic acid amplification |
| WO2022257944A1 (en) * | 2021-06-08 | 2022-12-15 | 南京金斯瑞生物科技有限公司 | Optical element detachable capillary clip and capillary electrophoresis instrument |
| CN117327841A (en) * | 2023-11-03 | 2024-01-02 | 山东省医学科学院基础医学研究所 | Method for jointly detecting monkey pox virus and chikungunya virus by capillary modified LAMP method |
-
2015
- 2015-08-17 CN CN201520617507.1U patent/CN204958934U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109307653A (en) * | 2017-07-27 | 2019-02-05 | 国家纳米科学中心 | Capillary detection platform based on ring mediated isothermal nucleic acid amplification |
| WO2022257944A1 (en) * | 2021-06-08 | 2022-12-15 | 南京金斯瑞生物科技有限公司 | Optical element detachable capillary clip and capillary electrophoresis instrument |
| CN117327841A (en) * | 2023-11-03 | 2024-01-02 | 山东省医学科学院基础医学研究所 | Method for jointly detecting monkey pox virus and chikungunya virus by capillary modified LAMP method |
| CN117327841B (en) * | 2023-11-03 | 2024-05-07 | 山东省医学科学院基础医学研究所 | Method for jointly detecting monkey pox virus and chikungunya virus by capillary modified LAMP method |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |