CN220969496U - Exosome separation extraction element - Google Patents
Exosome separation extraction element Download PDFInfo
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- CN220969496U CN220969496U CN202322836287.9U CN202322836287U CN220969496U CN 220969496 U CN220969496 U CN 220969496U CN 202322836287 U CN202322836287 U CN 202322836287U CN 220969496 U CN220969496 U CN 220969496U
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- centrifugal
- test tube
- separation
- extraction device
- exosome separation
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 41
- 238000000926 separation method Methods 0.000 title claims abstract description 28
- 238000000605 extraction Methods 0.000 title claims abstract description 15
- 238000012360 testing method Methods 0.000 claims abstract description 30
- 238000001914 filtration Methods 0.000 claims abstract description 23
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 239000011229 interlayer Substances 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 230000001681 protective effect Effects 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000000706 filtrate Substances 0.000 claims description 19
- 238000007789 sealing Methods 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 12
- 230000006978 adaptation Effects 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 239000010410 layer Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 description 9
- 238000001085 differential centrifugation Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000005374 membrane filtration Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 238000000703 high-speed centrifugation Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000000464 low-speed centrifugation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 206010061818 Disease progression Diseases 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000035992 intercellular communication Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Landscapes
- Centrifugal Separators (AREA)
Abstract
The utility model relates to the technical field of exosome separation, and particularly discloses an exosome separation and extraction device which comprises a centrifugal machine for exosome separation and a centrifugal test tube for loading liquid; the centrifugal machine comprises a protective cover and a machine body, wherein the protective cover is arranged above the machine body, and a driving part is arranged at the central position of the bottom of the inner side of the machine body; according to the utility model, the arranged centrifugal test tube is matched with the body arranged at the bottom of the centrifugal test tube, the circular truncated cones arranged in the annular array on the body are matched with the grooves at the bottom of the filtering component, the target object can be rapidly subjected to centrifugal separation in the rotating process of the centrifugal test tube after being injected into the centrifugal test tube by utilizing the matching, and the filtering effect of different cells can be realized by utilizing filtering membranes with different apertures in the interlayer of the limiting ring before separation.
Description
Technical Field
The utility model relates to the technical field of exosome separation, in particular to an exosome separation and extraction device.
Background
Isolation of exosomes is a method for extracting exosomes from cell cultures or biological fluids (e.g. plasma, urine, saliva, etc.). Exosomes are small vesicles secreted by cells and contain various biomolecules such as proteins, nucleic acids, lipids, and the like. Exosomes play an important role in intercellular communication, regulating physiological processes and participation in disease progression.
One common method for isolating exosomes is differential centrifugation. First, cell debris and large membranes were removed by low-speed centrifugation, and then, exosomes were precipitated using high-speed centrifugation.
Generally, after differential centrifugation, the target after centrifugation needs to be purified, so that the interference of other cells on exosomes is avoided as much as possible, and the operation mode is that liquid is extracted and filtered again by a filtering membrane;
By utilizing the membrane filtration, the problem that the filtration efficiency is reduced is solved because the filtration mode generally adopts a pressurized mode, at this time, the external body is possibly damaged externally, and the filtering hole of the membrane is blocked, and a procedure is added when liquid is extracted, so that a certain degree of waste and use are complicated, and the membrane filtration is more than once, and therefore, the membrane filtration needs to be improved.
Disclosure of utility model
Aiming at the defects of the prior art, the utility model provides an exosome separation and extraction device which is used for solving the problems proposed by the background technology.
The exosome separation and extraction device comprises a centrifugal machine for exosome separation and a centrifugal test tube for loading liquid;
The centrifugal machine comprises a protective cover and a machine body, wherein the protective cover is arranged above the machine body, a driving part is arranged at the central position of the bottom of the inner side of the machine body, a loading table is arranged at the top of the driving part, one or more test tube loading ports are formed in the annular shape at the top of the loading table, and the inner side of each test tube loading port is matched with a centrifugal test tube;
The centrifugal test tube comprises a body, the bottom of body is provided with filtrate collection department, the inboard of body is located the top department that filtrate was collected and is provided with the bottom plate, the top of bottom plate is provided with round or multiturn spacing groove for grafting filtration subassembly.
As a further improvement of the utility model, the filter assembly comprises an outer frame, an interlayer is arranged on the inner side of the outer frame, a filter membrane for filtering is arranged at the interlayer, a limiting ring is arranged at the top of the outer frame, and a material injection port is formed in the middle of the limiting ring.
As a further improvement of the utility model, the bottom of the limiting ring is provided with a convex ring, and the convex ring is matched with the interlayer and used for limiting the filter membrane.
As a further development of the utility model, the outer side of the outer frame is provided with one or more centrifugation areas in the form of a ring, which are adapted to the filter membrane for centrifugation of the object.
As a further improvement of the utility model, the bottom of the outer frame is provided with a combined ring, the inner side of the combined ring is provided with a groove, and the groove is matched with the boss.
As a further improvement of the utility model, the top of the body is provided with a sealing groove, and a sealing cover is sealed at the sealing groove for avoiding centrifugal splashing of the target.
As a further improvement of the utility model, a material guiding opening is arranged at the middle part of the bottom of the filtrate collecting part, and a sealing plug is arranged at the material guiding opening.
As a further improvement of the utility model, the bottom of the bottom plate is positioned at the outer side of the round table and is provided with a discharge hole in a ring shape, and the discharge hole is used for guiding the centrifuged target objects into a filtrate collecting position for converging.
Compared with the prior art, the utility model has the following beneficial effects:
According to the utility model, the centrifugal test tube is matched with the body arranged at the bottom of the centrifugal test tube, the circular truncated cones arranged in the annular array on the body are matched with the grooves at the bottom of the filtering component, the target object can be rapidly centrifugally separated in the process of rotating the centrifugal test tube after being injected into the centrifugal test tube by utilizing the matching, filtering effects of different cells can be realized by utilizing filtering membranes with different apertures in interlayers of the limiting rings before separation, finally, the remained exosomes can be guided out from the discharge hole arranged at the bottom plate and finally enter the filtrate collecting position, and then the purified exosomes can be collected by opening the sealing plug.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute a limitation on the application. In the drawings:
FIG. 1 is a schematic perspective view of a centrifuge according to the present utility model;
FIG. 2 is a schematic elevational view of the centrifuge of the present utility model;
FIG. 3 is a schematic view of the cross-sectional structure of the A-A in FIG. 2 according to the present utility model;
FIG. 4 is a schematic diagram of the front view of the centrifuge tube of the present utility model;
FIG. 5 is a schematic view of the cross-sectional structure A-A of FIG. 4 according to the present utility model;
FIG. 6 is a schematic top view of a centrifuge tube according to the present utility model;
FIG. 7 is an enlarged schematic view of the structure of FIG. 5A according to the present utility model;
FIG. 8 is a schematic view of the structure of the centrifuge tube and filter assembly of the present utility model;
FIG. 9 is a schematic perspective view of a filter assembly according to the present utility model;
FIG. 10 is a schematic elevational view of a filter assembly of the present utility model;
FIG. 11 is a schematic view of the cross-sectional structure A-A of FIG. 10 according to the present utility model.
In the figure: 1. a centrifuge; 2. centrifuging the test tube; 3. a filter assembly;
11. A protective cover; 12. a body; 13. a loading table; 14. a driving part; 15. a test tube loading port;
21. a body; 22. collecting filtrate; 23. sealing grooves; 24. a bottom plate; 25. round bench; 26. a discharge port; 27. a sealing plug;
31. A limiting ring; 32. a material injection port; 33. a convex ring; 34. an interlayer; 35. an outer frame; 36. a groove; 37. a combination ring; 38. and (5) a centrifugal area.
Detailed Description
Various embodiments of the present utility model are disclosed in the following drawings, which are presented in sufficient detail to provide a thorough understanding of the present utility model. However, it should be understood that these physical details should not be used to limit the utility model. That is, in some embodiments of the present utility model, these physical details are not necessary. Moreover, for the sake of simplicity of illustration, some well-known and conventional structures and components are shown in the drawings in a simplified schematic manner.
In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present utility model.
Referring to fig. 1, 2, 3, 4 and 8, one common method for separating exosomes is differential centrifugation. First, cell debris and large membranes were removed by low-speed centrifugation, and then, exosomes were precipitated using high-speed centrifugation.
Generally, after differential centrifugation, the target after centrifugation needs to be purified, so that the interference of other cells on exosomes is avoided as much as possible, and the operation mode is that liquid is extracted and filtered again by a filtering membrane;
When the membrane is used, the membrane is used for filtering, and the filtering mode generally adopts a pressurizing mode, at the moment, an exosome is possibly damaged externally, and a filtering hole of the membrane is blocked, so that the problem of reduced filtering efficiency is solved, a process is added when liquid is extracted, a certain degree of waste and complexity in use are caused, and the membrane is filtered more than once, so that the exosome separation and extraction device comprises a centrifugal machine 1 for exosome separation and a centrifugal test tube 2 for loading liquid;
The centrifugal machine 1 comprises a protective cover 11 and a machine body 12, wherein the protective cover 11 is arranged above the machine body 12, a driving part 14 is arranged at the central position of the bottom of the inner side of the machine body 12, a loading table 13 is arranged at the top of the driving part 14, one or more test tube loading ports 15 are formed in the annular shape at the top of the loading table 13, and the inner side of the test tube loading ports 15 is matched with the centrifugal test tube 2;
the centrifugal test tube 2 comprises a body 21, a filtrate collecting place 22 is arranged at the bottom of the body 21, a bottom plate 24 is arranged at the upper part of the filtrate collecting place 22 on the inner side of the body 21, and one or more circles of limiting grooves are formed in the top of the bottom plate 24 and used for being inserted into the filter assembly 3.
With this device, the liquid is first loaded into the centrifuge tube 2. Then, the test tube is inserted into the loading port, and the centrifuge 1 is rotated by the driving part 14, thereby achieving rapid centrifugation. Filtration of different cells in the limiting groove interlayer 34 can be achieved using filter assemblies 3 of different pore sizes prior to separation to leave the target material exosomes. The separated exosomes may be directed out through a discharge port 26 provided in the bottom plate 24 and into the filtrate collection 22. Finally, by opening the sealing plug 27, the purified exosomes can be conveniently collected. Compared with the traditional step-by-step filtration method, the device is simple and convenient to operate and high in efficiency, improves the collection efficiency of exosomes, and reduces the operation steps.
Referring to fig. 8, 9, 10 and 11, the filter assembly 3 includes an outer frame 35, an interlayer 34 is disposed on the inner side of the outer frame 35, a filter membrane for filtering is disposed on the interlayer 34, a limiting ring 31 is mounted on the top of the outer frame 35, and a material injection port 32 is formed in the middle of the limiting ring 31.
The bottom of the limiting ring 31 is provided with a convex ring 33, and the convex ring 33 is matched with the interlayer 34 and used for limiting the filter membrane.
The outer side of the outer frame 35 is provided with one or more centrifugation areas 38 in a ring shape, and the centrifugation areas 38 are adapted to the filter membrane for centrifuging the target.
The bottom of frame 35 is provided with combination ring 37, and recess 36 has been seted up to the inboard of combination ring 37, recess 36 and boss adaptation.
In use, the filter assembly 3 is inserted into the limiting groove of the bottom plate 24 of the centrifugal test tube 2, ensuring that the collar 33 is fitted into the limiting groove of the bottom plate 24. The mixed liquid to be treated is then added to the filter assembly 3 through the filler opening 32. During centrifugation, the centrifuge tube 2 rotates with the filter assembly 3 to subject the liquid to centrifugal force, thereby separating the target substances such as exosomes into the interlayer 34 above the filter membrane and filtering out other impurities. The separated target objects can be conveniently collected and extracted by opening the discharge port 26 of the bottom plate 24 of the centrifugal test tube 2.
The filter assembly 3 with the design can realize efficient separation of samples and ensure stable and closed position of the filter membrane so as to avoid interference of impurities. At the same time, the design of the combining ring 37 ensures a good adaptation of the filter assembly 3 to the bottom plate 24 of the centrifuge tube 2, providing reliable operation performance.
Referring to fig. 1, 2 and 3, a sealing groove 23 is formed at the top of the body 21, and a sealing cover is sealed at the sealing groove 23 to avoid centrifugal splashing of the target.
A material guide hole is formed in the middle of the bottom of the filtrate collecting position 22, and a sealing plug 27 is arranged at the material outlet 26.
Referring to fig. 4, 5, 6, 7 and 8, a discharge hole 26 is formed in a ring shape at the outer side of the circular table 25 at the bottom of the bottom plate 24, and is used for guiding the centrifuged target objects into the filtrate collecting place 22 for collecting.
The body 21 top of centrifugal test tube 2 has offered seal groove 23 to be provided with sealed lid for effectively prevent that the target object from splashing in the centrifugation process. This protects the laboratory environment from cleaning and sample loss.
A material guiding opening is arranged at the middle part of the bottom of the filtrate collecting part 22, and a sealing plug 27 is arranged at the material guiding opening. The centrifuged target is guided to the filtrate collecting place 22 through the feed port, and the purified exosomes are conveniently collected and extracted by opening the sealing plug 27.
The bottom outer side of the bottom plate 24 is annularly provided with a discharge hole 26 for guiding the centrifuged target objects into the filtrate collecting place 22, so as to realize rapid convergence of the target objects. Thus, the exosome after centrifugal separation can smoothly flow into the filtrate collecting place 22, and the subsequent collection and treatment are convenient.
The foregoing description is only illustrative of the utility model and is not to be construed as limiting the utility model. Various modifications and variations of the present utility model will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, or the like, which is within the spirit and principle of the present utility model, should be included in the scope of the claims of the present utility model.
Claims (8)
1. An exosome separation and extraction device comprises a centrifugal machine (1) for exosome separation and a centrifugal test tube (2) for loading liquid;
The method is characterized in that:
The centrifugal machine (1) comprises a protective cover (11) and a machine body (12), wherein the protective cover (11) is arranged above the machine body (12), a driving component (14) is arranged at the central position of the bottom of the inner side of the machine body (12), a loading table (13) is arranged at the top of the driving component (14), one or more test tube loading ports (15) are formed in the annular shape at the top of the loading table (13), and the inner side of the test tube loading ports (15) is matched with a centrifugal test tube (2);
The centrifugal test tube (2) comprises a body (21), a filtrate collecting part (22) is arranged at the bottom of the body (21), a bottom plate (24) is arranged at the position above the filtrate collecting part (22) on the inner side of the body (21), and one or more circles of limiting grooves are formed in the top of the bottom plate (24) and used for being inserted into a filtering assembly (3).
2. An exosome separation and extraction device according to claim 1, wherein: the filter assembly (3) comprises an outer frame (35), an interlayer (34) is arranged on the inner side of the outer frame (35), a filter membrane for filtering is arranged at the interlayer (34), a limiting ring (31) is arranged at the top of the outer frame (35), and a material injection opening (32) is formed in the middle of the limiting ring (31).
3. An exosome separation and extraction device according to claim 2, wherein: the bottom of spacing ring (31) is provided with bulge loop (33), bulge loop (33) and intermediate layer (34) adaptation are used for restricting the filter membrane.
4. An exosome separation and extraction device according to claim 2, wherein: the outer side of the outer frame (35) is annularly provided with one or more centrifugal areas (38), and the centrifugal areas (38) are matched with the filter membrane and are used for carrying out centrifugal separation on a target object.
5. An exosome separation and extraction device according to claim 2, wherein: the bottom of frame (35) is provided with combination ring (37), recess (36) have been seted up to the inboard of combination ring (37), recess (36) and boss adaptation.
6. An exosome separation and extraction device according to claim 1, wherein: a sealing groove (23) is formed in the top of the body (21), and a sealing cover is sealed at the sealing groove (23) and used for avoiding centrifugal splashing of a target object.
7. An exosome separation and extraction device according to claim 1, wherein: a material guiding opening is formed in the middle of the bottom of the filtrate collecting part (22), and a sealing plug (27) is arranged at the material guiding opening.
8. An exosome separation and extraction device according to claim 1, wherein: the bottom of the bottom plate (24) is positioned at the outer side of the round table (25) and is provided with a discharge hole (26) in a ring shape, and the discharge hole is used for guiding the centrifuged target objects into the filtrate collecting place (22) for converging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322836287.9U CN220969496U (en) | 2023-10-21 | 2023-10-21 | Exosome separation extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322836287.9U CN220969496U (en) | 2023-10-21 | 2023-10-21 | Exosome separation extraction element |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220969496U true CN220969496U (en) | 2024-05-17 |
Family
ID=91060962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322836287.9U Active CN220969496U (en) | 2023-10-21 | 2023-10-21 | Exosome separation extraction element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220969496U (en) |
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2023
- 2023-10-21 CN CN202322836287.9U patent/CN220969496U/en active Active
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A device for separating and extracting extracellular vesicles Granted publication date: 20240517 Pledgee: Guanggu Branch of Wuhan Rural Commercial Bank Co.,Ltd. Pledgor: Wuhan liangpei medical laboratory Co.,Ltd. Registration number: Y2024980025503 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |