CN116064218B - Intelligent device for enriching abnormal blood large cells - Google Patents
Intelligent device for enriching abnormal blood large cells Download PDFInfo
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- CN116064218B CN116064218B CN202310359819.6A CN202310359819A CN116064218B CN 116064218 B CN116064218 B CN 116064218B CN 202310359819 A CN202310359819 A CN 202310359819A CN 116064218 B CN116064218 B CN 116064218B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/08—Rotary bowls
- B04B7/12—Inserts, e.g. armouring plates
- B04B7/16—Sieves or filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The application discloses an intelligent device for enriching abnormal blood large cells, which comprises a centrifugal disc which is driven to rotate, wherein a plurality of centrifugal holes are annularly formed in the centrifugal disc, a filter cavity and a storage cavity are arranged in the centrifugal holes, the filter cavity is communicated with the storage cavity, a filter membrane is arranged in the filter cavity, during centrifugation, liquid to be centrifuged is filled in the filter cavity, waste liquid passes through the filter membrane and enters the storage cavity during centrifugation, and the intelligent device for enriching abnormal blood large cells is disclosed by the application.
Description
Technical Field
The application relates to a blood cell enrichment technology, in particular to an intelligent device for enriching abnormal large blood cells.
Background
Blood cells, also known as "blood cells", are cells that are present in blood and that, as blood flows throughout the body, are essentially of the following three species in the case of mammals: in the process of canceration of normal cells, the characteristics of increased cell volume, increased nuclear-plasma ratio, deep dyeing of chromatin and the like usually occur, and the canceration large cells formed in the canceration process are called abnormal large cells, so in the prior art, by extracting the abnormal cells from peripheral blood, and performing a series of subsequent experiments such as staining, identification, analysis, qualitative and the like after separation, the advanced index is provided for corresponding canceration of a human body by judging whether the enriched abnormal large cells are circulating tumor cells or not.
The prior art has a collecting device for abnormal large cells, for example, chinese patent application No. CN201910590228.3, and the application patent application with the publication date of 2019.07.02 discloses a system for enriching abnormal large cells in blood and a buffering and filtering device thereof, comprising a sampling device, a buffering device, a filtering device and a negative pressure device, wherein the sampling device is used for collecting blood and obtaining a blood sample; the buffer device is internally provided with buffer solution and is used for acquiring a blood sample in the sampling device and carrying out buffer treatment on the blood sample to obtain a buffered blood sample; the filter device is internally provided with a filter membrane, is matched with the buffer device and is used for filtering the buffered blood sample to obtain abnormal large cells; the negative pressure device is matched with the filtering device and is used for providing negative pressure during filtering. The application application eliminates sampling interference; step-by-step release of buffer and rinsing; the device is convenient to disassemble, has a simple structure, and is favorable for enriching abnormal large cells and matching with subsequent experimental treatments such as dyeing analysis and identification; the devices are directly connected, so that pollution caused by extra links is avoided.
In the prior art including the patent, blood cells are separated and layered in a centrifugal way in the prior art, and then the next target abnormal large cells are extracted through an extracting device, so that the operation is complicated.
Disclosure of Invention
The application aims to provide an intelligent device for enriching abnormal blood large cells, so as to solve the defects in the prior art.
In order to achieve the above object, the present application provides the following technical solutions:
the utility model provides an enrichment blood abnormal large cell's intelligent device, includes driven rotatable centrifugal disk, be annular a plurality of centrifugal holes that are equipped with on the centrifugal disk, be equipped with blood in the centrifugal hole and hold the piece, be formed with filter chamber and storage chamber in the blood holds the piece, the filter chamber with the storage chamber intercommunication, be equipped with the filter membrane in the filter chamber, during centrifugation, wait to centrifuge liquid level in the filter chamber, centrifugation in-process waste liquid passes the filter membrane gets into in the storage chamber.
The above-mentioned intelligent device of enrichment blood abnormal large cell, blood holds the piece and is the cartridge filter, the cartridge filter is used for gathering the blood sample, the cartridge filter is placed in the centrifugation downthehole, intermediate position is equipped with the support in the cartridge filter, the filter membrane sets up on the support.
The intelligent device for enriching the abnormal blood large cells further comprises a separation mechanism, wherein the separation mechanism is used for extracting waste liquid in the filter cartridge under the centrifugal action.
The above-mentioned intelligent device of unusual big cell of enrichment blood, separating mechanism includes baffle, storage section of thick bamboo and control lever, the baffle is established in the centrifugation is downthehole, first through-hole has been seted up on the baffle surface, the storage section of thick bamboo sets up in the centrifugation just sets up the baffle below, the interior dynamic seal of storage section of thick bamboo is equipped with the piston plate, be equipped with first spring on the piston plate, first spring is located the piston plate below is used for resetting the piston plate, the storage section of thick bamboo upper end is equipped with feed liquor pipe and drain pipe, feed liquor pipe one end is equipped with the tail needle, the tail needle passes through first through-hole stretches out, the drain pipe stretches out the centrifugation is downthehole, the control lever runs through the feed liquor pipe with on the drain pipe, the control lever is located the feed liquor pipe with the inside position of drain pipe is equipped with first valve plate and second valve plate respectively, first valve plate with second valve plate mutually perpendicular, be equipped with drive assembly in the centrifugation downthehole, thereby drive assembly is used for driving control lever first valve plate and second valve plate realization the feed liquor pipe is turned over.
The intelligent device for enriching abnormal blood large cells comprises a driving assembly, wherein the driving assembly comprises a first sliding trapezoidal block, the first sliding trapezoidal block moves linearly in the axial direction in a centrifugal hole, a second spring is arranged on the first sliding trapezoidal block, one end of the second spring is fixed on the inner wall of the storage cylinder, the second spring is used for resetting the first sliding trapezoidal block, a first rack is arranged on the first sliding trapezoidal block, a first gear is arranged on a control rod, and the first rack is meshed with the first gear.
The intelligent device for enriching the abnormal blood large cells further comprises a blending mechanism, wherein the blending mechanism is used for controlling the piston plate to move under the centrifugal action to extract the waste liquid in the filter cartridge.
The above-mentioned intelligence device of unusual big cell of enrichment blood, allotment mechanism includes first branch, second branch and second trapezoidal piece, first branch one end is fixed piston board bottom surface, the second branch slides and cup joints on the first branch, the second branch is kept away from the one end rotation of first branch is connected the downthehole bottom of centrifugation, Z type groove has been seted up on the second branch surface, first branch one end is equipped with the bellying, the bellying is in Z type inslot, the inside second spring that is equipped with of second branch, first branch one end is supported on the second spring, the cover has the second gear on the second branch, second trapezoidal piece sliding connection is in downthehole bottom and radial rectilinear motion at the centrifugal disk, be equipped with the second rack on the second trapezoidal piece, the second rack with the second gear engagement, be equipped with the third spring on the second trapezoidal piece, third spring one end is fixed be used for carrying out reset to the second trapezoidal piece on the inner wall of a storage section of thick bamboo, two trapezoidal pieces are parallel to each other with the second trapezoidal piece that slides, two trapezoidal pieces are parallel to set up each other.
The intelligent device for enriching abnormal blood large cells further comprises a box body, wherein the centrifugal disc is arranged in the box body, and a box cover is arranged on the box body and used for covering the centrifugal holes.
The intelligent device for enriching abnormal blood large cells further comprises a cleaning mechanism, wherein the cleaning mechanism is arranged on the centrifugal disc, the cleaning mechanism comprises an electric push rod, a cleaning cavity is formed in the middle of the centrifugal disc, the cleaning cavity is communicated with each through hole of the centrifugal hole, the electric push rod is arranged in the cleaning cavity, a sealing plate is arranged at the output end of the electric push rod, and the sealing plate is connected in the cleaning cavity in a dynamic sealing mode.
The intelligent device for enriching the abnormal large blood cells is characterized in that the inner diameter of the storage cylinder is larger than that of the blood containing piece.
The application has the beneficial effects that: the blood is filtered through the filter membrane in the filter cavity under the action of centrifugal force, so that target cells are left on the filter membrane, the operation of extracting abnormal large cells among different components is omitted, and the operation is convenient.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.
FIG. 1 is a schematic diagram of a smart device for enriching abnormal blood large cells according to an embodiment of the present application;
FIG. 2 is a schematic diagram of a centrifugal disk structure according to an embodiment of the present application;
FIG. 3 is a schematic view of the internal structure of a centrifugal hole according to an embodiment of the present application;
FIG. 4 is a schematic view of a control lever according to another embodiment of the present application;
FIG. 5 is a schematic view of an open structure of a filter cartridge according to another embodiment of the present application;
fig. 6 is a schematic structural diagram of a blending mechanism according to another embodiment of the present application.
Reference numerals illustrate:
1-a centrifuge disk; 2-filtering membrane; 3-a filter cartridge; 4-a storage cylinder; 41-a piston plate; 42-liquid inlet pipe; 421-tail needle; 43-a liquid outlet pipe; 44-a control lever; 51-a first valve plate; 52-a second valve plate; 53-a first gear; 61-a first slide block; 611-a first rack; 71-a first strut; 72-a second strut; 73-a second trapezoidal block; 74-a boss; 75-a second gear; 8, a box body; 9-an electric push rod; 91-sealing plate.
Detailed Description
In order to make the technical scheme of the present application better understood by those skilled in the art, the present application will be further described in detail with reference to the accompanying drawings.
As shown in fig. 1-6, the intelligent device for enriching abnormal blood large cells provided by the embodiment of the application comprises a centrifugal disc 1 which is driven to rotate, wherein a plurality of centrifugal holes are formed in the centrifugal disc 1 in a ring shape, a blood containing piece is formed in the centrifugal holes, a filter cavity and a storage cavity are formed in the blood containing piece, the filter cavity is communicated with the storage cavity, a filter membrane 2 is arranged in the filter cavity, liquid to be centrifuged is positioned in the filter cavity during centrifugation, and waste liquid passes through the filter membrane 2 in the centrifugation process and enters the storage cavity.
Specifically, the centrifugal hole is inclined towards the central axis of the centrifugal disc 1, namely, the bottom end of the centrifugal hole is far away from the central axis, the top end of the centrifugal hole is close to the central axis, namely, the opening of the centrifugal hole is close to the central axis, a blood containing part is arranged in the centrifugal hole, the blood containing part is a blood containing structure, generally a cylinder body, in the embodiment, the centrifugal hole can be used for directly injecting blood into the centrifugal hole under the optional condition, at this moment, the centrifugal hole is thoroughly cleaned after each operation, a limiting part such as a step structure is arranged at the position between a filter cavity and a storage cavity in the blood containing part, a filter membrane 2 is a whole blood separation membrane and is also called a hemofiltration membrane, red blood cells and white blood cells in whole blood are trapped in a physical separation mode, the filter membrane 2 is not described in the prior art, a hard grid is arranged at the bottom surface of the filter membrane 2 for supporting so as to prevent tearing during high-speed centrifugation, for example, the filter membrane 2 is paved on the round cover container, one surface of the filter membrane 2 with the hard grid is placed into the centrifugal hole and supported on the limiting part, the whole blood is directly placed into the round cover container under the action of the filter membrane 2, and the filter membrane is directly placed into the round cover container under the action of the round cover 2 after the abnormal cell is directly placed into the round cover container, and the blood component is directly pulled out from the round cover under the action of the round cover, and the filter membrane is removed from the abnormal component under the action of the round cover, and the filter membrane is removed from the round cover.
The embodiment of the application has the beneficial effects that: the permeation speed of blood on the filter membrane 2 is accelerated through centrifugation, blood cells can be rapidly extracted, and the operation is convenient.
In still another embodiment of the present application, the blood containing member is a filter cartridge 3, where the filter cartridge 3 is used for containing a blood sample, preferably, the filter cartridge 3 may be directly improved on a blood collection tube in the prior art, a limit structure is disposed in the middle of the filter cartridge 3 and a filter membrane 2 is disposed, the filter cartridge 3 is placed in the centrifugal hole, a support is disposed in the middle position in the filter cartridge 3, the support is a porous disc body, the filter membrane 2 is disposed on the support, the filter cavity is on the side with the filter membrane 2 on the support, and the storage cavity is on the other side of the support.
Specifically, insert the blood taking needle puncture end in waiting the sample person's vein, the blood taking needle tail end inserts in the plug of cartridge filter 3 and takes a sample, blood gets into in cartridge filter 3, can prestore the buffer in cartridge filter 3, after the sample, rock and dilute blood in the cartridge filter 3, put into the centrifugation downthehole with cartridge filter 3 afterwards, under the centrifugal effect, blood infiltration filter membrane 2 accomplishes the filtration, the waste liquid remains in cartridge filter 3, wait after the centrifugation, take out cartridge filter 3, unscrew the end cover, take out the support, can extract filter membrane 2.
The above embodiment has a certain disadvantage, the separated waste liquid still exists in the filter cartridge 3, some uncontrollable factors such as shaking and dropping generated in the subsequent operation of completing the separation can cause pollution caused by the waste liquid contacting the filter membrane 2 again, and the waste liquid remains in the filter cartridge 3 and is inconvenient for recycling the filter cartridge 3.
In a further embodiment, the application further comprises a passive separation mechanism for extracting the waste liquid in the filter cartridge 3 under the centrifugal action.
In still another embodiment of the present application, the separating mechanism includes a partition board, a storage cylinder 4 and a control rod 44, where the partition board is disposed in a centrifugal hole, a first through hole is formed on a surface of the partition board, the storage cylinder 4 is disposed in the centrifugal hole and below the partition board, a piston plate 41 is disposed in the storage cylinder 4 in a dynamic sealing manner, a first spring is disposed on the piston plate 41, the first spring is disposed below the piston plate 41 and is used for resetting the piston plate 41, a liquid inlet pipe 42 and a liquid outlet pipe 43 are disposed at an upper end of the storage cylinder 4, a tail needle 421 is disposed at one end of the liquid inlet pipe 42, the tail needle 421 extends out through the first through hole, the liquid outlet pipe 43 extends out of the centrifugal hole, the control rod 44 penetrates through the liquid inlet pipe 42 and the liquid outlet pipe 43, a first valve plate 51 and a second valve plate 52 are disposed at positions in the liquid inlet pipe 42 and the liquid outlet pipe 43, the first valve plate 51 and the second valve plate 52 are disposed vertically to each other, and the first valve plate 51 and the second valve plate 52 are disposed vertically to drive the liquid inlet pipe 44 and the liquid outlet pipe 43 to turn over the first valve plate and the second valve plate 52.
Specifically, the first valve plate 51 and the second valve plate 52 are structured like valve plates of butterfly valves in the prior art, sealing pieces are arranged inside the liquid inlet pipe 42 and the liquid outlet pipe 43, the first valve plate 51 and the second valve plate 52 respectively seal the liquid inlet pipe 42 and the liquid outlet pipe 43 when contacting the corresponding sealing pieces during rotation, the sealing pieces have certain limiting function, rubber plug parts are arranged at the upper end and the lower end of the adopted filter cartridge 3 in the embodiment, the part length of the tail needle 421 extending out of the first through hole is slightly larger than the thickness of the rubber plug part, so that the tail needle 421 can penetrate through the rubber plug part and can not extend into the filter cartridge 3 excessively, the liquid inlet pipe 42 is closed in the initial state of the first valve plate 51, the liquid outlet pipe 43 is opened in the initial state of the second valve plate 52, the rotation speed of the centrifugal disc 1 has two gears of low speed and high speed, the rubber plug part at one end of the filter cartridge 3 after blood collection is aligned with the tail needle 421 to be inserted and fixed, the centrifugal disc 1 rotates at a low speed, blood at the upper part of the filter cartridge 3 enters the inner bottom part of the filter cartridge 3 through the filter membrane 2, the centrifugal force borne by the piston plate 41 is smaller and has no obvious displacement under the low speed, then the centrifugal disc 1 enters a high speed, the driving component drives the control rod 44 to rotate so as to open the first valve plate 51 and close the second valve plate 52, the liquid inlet pipe 42 is opened, the liquid outlet pipe 43 is closed, under the high speed, the centrifugal force borne by the piston plate 41 is increased and is far greater than the elastic force of the first spring at the bottom part of the piston plate 41, the piston plate 41 starts to move downwards so as to suck waste liquid in the filter cartridge 3 into the storage cylinder 4 from the liquid inlet pipe 42, then the centrifugal disc 1 stops working, the driving component drives the control rod 44 to rotate so as to close the first valve plate 51 and open the second valve plate 52, the liquid inlet pipe 42 is closed, and the liquid outlet pipe 43 is opened, the piston plate 41 is reset under the action of the first spring, waste liquid above the piston plate 41 is discharged through the liquid outlet pipe 43, and when the centrifugal disc 1 stops, the filter membrane 2 can be taken out by taking down the filter cartridge 3 and unscrewing the box cover.
Preferably, the blind hole is formed in the rubber plug portion, the blind hole does not penetrate the rubber plug portion, the depth of the blind hole is smaller than the length of the tail needle 421 extending out of the first through hole portion, the blind hole in the rubber plug portion at the bottom of the filter cartridge 3 is aligned with the tail needle 421 for insertion, centrifugal force is insufficient to enable the tail needle 421 to pierce the rubber plug portion when the centrifugal force is low-speed, at this time, when the centrifugal force is high-speed, the filter cartridge 3 moves downwards under the centrifugal action to enable the tail needle 421 to pierce the rubber plug portion and extend into the filter cartridge 3 for extracting waste liquid.
In this embodiment, the low speed and the high speed correspond to different states, such as whether the tail needle 421 can pierce the rubber plug portion, and the rotation speed corresponding to the low speed and the high speed can be obtained through a limited number of experiments, which is a conventional experimental means in the art, and specific processes and rotations are not repeated in this embodiment.
The embodiment of the application has the beneficial effects that: the waste liquid separated from the filter cartridge 3 is extracted through the separating mechanism, so that the waste liquid is convenient to collect, and the residual waste liquid in the filter cartridge 3 is less and does not pollute target cells collected on the surface of the filter membrane 2, so that the filter cartridge 3 is convenient to recover.
In still another embodiment of the present application, the driving assembly includes a first sliding trapezoid block 61, the first sliding trapezoid block 61 is limited to move linearly in the axial direction in the centrifugal hole, a second spring is disposed on the first sliding trapezoid block 61, one end of the second spring is fixed on the inner wall of the storage cylinder 4, the second spring is used for resetting the first sliding trapezoid block 61, a first rack 611 is disposed on the first sliding trapezoid block 61, a first gear 53 is disposed on the control rod 44, and the first rack 611 is meshed with the first gear 53.
Specifically, when the centrifugal disc 1 rotates at the low gear, the first sliding trapezoid block 61 cannot compress the second spring to obviously move due to the small centrifugal force, at this time, blood in the filter cartridge 3 is filtered under the centrifugal action, then the centrifugal disc 1 enters into the high gear, at the high gear, the first sliding trapezoid block 61 moves downwards, the first rack 611 on the first sliding trapezoid block 61 drives the first gear 53 on the control rod 44 to rotate so as to open the first valve plate 51 and close the second valve plate 52, at the moment, the liquid inlet pipe 42 is opened, the liquid outlet pipe 43 is closed, the piston plate 41 starts to move downwards under the centrifugal action under the high gear so as to suck waste liquid in the filter cartridge 3 into the storage cylinder 4 from the liquid inlet pipe 42, then the working speed of the centrifugal disc 1 is gradually reduced, the first sliding trapezoid block 61 starts to move upwards so as to close the first valve plate 51 and open the second valve plate 52, and the piston plate 41 resets under the action of the first spring so as to press the blood sucked into the liquid outlet pipe 43 into the liquid storage cylinder 4 into the liquid storage bin.
The above embodiment still has a certain disadvantage that the first sliding block 61 and the piston plate 41 start to move simultaneously during the deceleration of the centrifugal disc 1, which easily causes that the liquid outlet pipe 43 or the liquid inlet pipe 42 does not complete the opening and closing operation yet the piston plate 41 starts to move, so that the piston plate 41 draws air from the liquid outlet pipe 43 or presses the waste liquid in the storage cylinder 4 back into the filter cartridge 3, since the centrifugal force is directly proportional to the rotation speed of the centrifugal disc 1, the centrifugal force in the high-speed gear needs to force the piston plate 41 to compress the first spring for a larger stroke, and the piston plate 41 is basically not moved in the low-speed gear, which needs to be far larger than in the low-speed gear, and the rotation speed is excessively large, which easily causes that the movement state of the centrifugal disc 1 is uncontrollable during the high-speed operation, and the piston plate 41 and the first sliding block 61 depend on the centrifugal force to change the movement state, which causes that the movement cooperation of the piston plate 41 and the first sliding block 61 cannot be completely synchronized, and therefore a deployment mechanism is needed to cooperate with the process.
In yet another embodiment of the present application, a dispensing mechanism is further included for controlling the movement of the piston plate 41 under centrifugal action to extract the waste liquid from the filter cartridge 3.
In still another embodiment of the present application, the deployment mechanism includes a first supporting rod 71, a second supporting rod 72 and a second trapezoid block 73, one end of the first supporting rod 71 is fixed on the bottom surface of the piston plate 41, the second supporting rod 72 is slidably sleeved on the bottom of the first supporting rod 71, one end of the second supporting rod 72, which is far away from the first supporting rod 71, is rotatably connected to the bottom of the centrifugal hole, a Z-shaped groove is formed on the surface of the second supporting rod 72, one end of the first supporting rod 71 is provided with a protruding portion 74, the protruding portion 74 is located in the Z-shaped groove, a third spring is disposed inside the second supporting rod 72, one end of the first supporting rod 71 is abutted against the third spring, a second gear 75 is sleeved on the second supporting rod 72, the second trapezoid block 73 is slidably connected to the bottom of the centrifugal hole and is limited to move linearly in the radial direction of the centrifugal disk 1, a second rack 76 is meshed with the second gear 75, a fourth rack 76 is disposed on the second trapezoid block 73, a fourth spring is disposed on the second trapezoid block 73, and the second trapezoid block 73 is in parallel to the inner wall of the first trapezoid block 61.
Specifically, in this embodiment, the first gear 53 is required to be rotationally connected to the control rod 44 and has a certain rotational resistance, when the first valve plate 51 or the second valve plate 52 on the control rod 44 is not closed, the first rack 611 will drive the first gear 53 to rotate simultaneously with the control rod 44, and when the first valve plate 51 or the second valve plate 52 on the control rod 44 is closed and the control rod 44 cannot rotate, the first rack 611 will drive the first gear 53 to rotate on the control rod 44.
Specifically, when the centrifugal disk 1 rotates at a low speed, blood in the filter cartridge 3 starts to enter the bottom of the filter cartridge 3 through the filter membrane 2, the first sliding trapezoidal block 61 compresses the third spring to move downwards under the action of centrifugal force to press the second trapezoidal block 73 (as shown in fig. 3), the first sliding trapezoidal block 61 moves downwards and drives the first gear 53 on the control rod 44 to rotate, at the moment, the first valve plate 51 and the second valve plate 52 rotate in a small range, because the centrifugal force is smaller at the low speed, the second valve plate 52 is not fully opened, the second trapezoidal block 73 starts to move along the radial direction of the centrifugal disk 1, the second gear 75 on the second supporting rod 72 is pulled by the second rack 76 on the second trapezoidal block 73 to rotate, at the moment, the protruding part 74 on the first supporting rod 71 moves relatively in the horizontal section of the Z-shaped groove and does not enter the vertical section of the Z-shaped groove, at the moment, the piston plate 41 remains motionless, when the centrifugal disc 1 enters the high-speed gear, the first sliding trapezoid block 61 continuously moves downwards to press the second trapezoid block 73, at the moment, the first valve plate 51 is completely closed, the second valve plate 52 is completely opened, at the moment, the protruding part 74 just enters the vertical section of the Z-shaped groove, the protruding part 74 on the first supporting rod 71 at the bottom of the piston plate 41 breaks away from the limit, the instant compression third spring moves downwards to suck waste liquid in the filter cartridge 3 into the storage cylinder 4 from the liquid inlet pipe 42, the protruding part 74 is positioned in the vertical section of the Z-shaped groove during the downward movement of the piston plate 41, the second supporting rod 72 cannot rotate, the first sliding trapezoid block 61 and the second trapezoid block 73 are mutually locked and kept motionless, when the piston plate 41 reaches the bottommost position in the storage cylinder 4, the protruding part 74 on the first supporting rod 71 reaches the horizontal section position below the Z-shaped groove, the first sliding trapezoid block 61 continuously moves downwards, because the second valve plate 52 in the liquid outlet pipe 43 is in a closed state, the second valve plate 52 is limited by the sealing piece and can not rotate, the control rod 44 can not continue to rotate, the first rack 611 on the first trapezoid block 61 drives the first gear 53 to rotate, the first trapezoid block 61 pushes the second trapezoid block 73 to move so as to drive the second supporting rod 72 to rotate, the boss 74 enters the horizontal section of the Z-shaped groove again, at the moment, the piston plate 41 is locked at the bottommost part in the storage cylinder 4, then the centrifugal disc 1 starts to slow down and stop, the centrifugal force borne by the first trapezoid block 61 gradually decreases and starts to move upwards, the first trapezoid block 61 returns upwards to drive the first gear 53 on the control rod 44 to rotate so as to close the first valve plate 51 and simultaneously open the second valve plate 52, at the moment, the liquid inlet pipe 42 is closed, the liquid outlet pipe 43 is opened, the second trapezoid block 73 is attached to the first trapezoid block 61 and is far away from the second supporting rod 72 along the radial direction of the centrifugal disc 1, the second trapezoid block 73 drives the second supporting rod 72 to rotate so as to rotate the boss 74 to the vertical section of the piston plate 41 in the Z-shaped groove, the reset spring block 41 is acted on the lower part of the third spring block 4, and the second trapezoid block 61 is driven to move upwards along the radial direction of the second supporting rod 72, and the second trapezoid block 72 is kept away from the second trapezoid block 72 along the radial direction of the second supporting rod 72, and the second trapezoid block 61 is kept upwards, and the second trapezoid block 61 is kept far away from the second supporting rod 72, at the second supporting rod is kept upwards, and the second supporting rod is kept away from the second supporting rod 72, and the second supporting rod 72, and is kept.
The embodiment of the application has the beneficial effects that: the mixing mechanism enables the piston plate 41 to correspondingly move after the liquid outlet pipe 43 or the liquid inlet pipe 42 completes the opening and closing actions, so that the problem that the piston plate 41 pumps air or returns waste liquid to the filter cartridge 3 is avoided.
In still another embodiment of the present application, the filter cartridge further includes a housing 8, the centrifugal disc 1 is disposed in the housing 8, a cover is disposed on the housing 8, the bottom of the housing 8 is detachable for pouring waste liquid, and the cover is used for covering the centrifugal hole to prevent the filter cartridge 3 from shaking in the centrifugal hole.
In still another embodiment of the present application, the cleaning mechanism is disposed on the centrifugal disc 1, the cleaning mechanism includes an electric push rod 9, a cleaning cavity is provided in the middle of the centrifugal disc 1, the cleaning cavity is communicated with each through hole of the centrifugal hole, the electric push rod 9 is disposed in the cleaning cavity, a sealing plate 91 is disposed at an output end of the electric push rod 9, the sealing plate 91 is dynamically and hermetically connected in the cleaning cavity, and a sealing plug is disposed at an opening of the cleaning cavity for supplementing cleaning liquid in the cleaning cavity.
Specifically, when the blood-holding member on the centrifugal disk 1 is filtered and taken out, the electric push rod 9 pushes the sealing plate 91 upward, the cleaning liquid enters the centrifugal hole, and then the centrifugal disk 1 completes the filtering operation once again to flush the pipe and the cavity with the cleaning liquid.
In still another embodiment of the present application, the inner diameter of the storage cylinder 4 is larger than the inner diameter of the blood containing member, and the storable volume in the storage cylinder 4 is much larger than the blood containing member volume, so that the displacement distance of the piston plate 41 is reduced, the maximum rotation speed of the centrifugal disk 1 is reduced, and the stability is improved.
While certain exemplary embodiments of the present application have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that modifications may be made to the described embodiments in various different ways without departing from the spirit and scope of the application. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive of the scope of the application, which is defined by the appended claims.
Claims (7)
1. An intelligent device for enriching abnormal blood large cells, which comprises a driven rotatable centrifugal disk, wherein a plurality of centrifugal holes are annularly arranged on the centrifugal disk,
a blood containing part is arranged in the centrifugal hole, a filter cavity and a storage cavity are formed in the blood containing part, the filter cavity is communicated with the storage cavity, a filter membrane is arranged in the filter cavity,
during centrifugation, liquid to be centrifuged is placed in the filter cavity, and waste liquid passes through the filter membrane and enters the storage cavity during centrifugation;
the blood containing piece is a filter cartridge, the filter cartridge is used for containing blood samples, the filter cartridge is placed in the centrifugal hole, a bracket is arranged in the middle position in the filter cartridge, and the filter membrane is arranged on the bracket;
the separating mechanism is used for extracting waste liquid in the filter cartridge under the centrifugal action, the separating mechanism comprises a partition plate, a storage cylinder and a control rod, the partition plate is arranged in the centrifugal hole, a first through hole is formed in the surface of the partition plate, the storage cylinder is arranged in the centrifugal hole and below the partition plate, a piston plate is dynamically sealed in the storage cylinder, a first spring is arranged on the piston plate, the first spring is positioned below the piston plate and used for resetting the piston plate, a liquid inlet pipe and a liquid outlet pipe are arranged at the upper end of the storage cylinder, a tail needle is arranged at one end of the liquid inlet pipe and extends into the filter cavity through the first through hole, the liquid outlet pipe extends out of the centrifugal hole, the control rod penetrates through the liquid inlet pipe and the liquid outlet pipe, a first valve plate and a second valve plate are respectively arranged at positions inside the liquid inlet pipe and the liquid outlet pipe, the first valve plate and the second valve plate are mutually perpendicular, a liquid inlet pipe and a liquid outlet pipe are driven by the control rod, and the liquid inlet pipe and the liquid outlet pipe are driven by the control rod to rotate;
the upper end and the lower end of the filter cylinder are respectively provided with a rubber plug part, the length of the part of the tail needle extending out of the first through hole is larger than the thickness of the rubber plug part, so that the tail needle can penetrate through the rubber plug part, the liquid inlet pipe is closed in the initial state of the first valve plate, the liquid outlet pipe is opened in the initial state of the second valve plate, the rotating speed of the centrifugal disc is provided with two gears of low speed and high speed, the rubber plug part at one end of the filter cylinder for collecting blood is aligned with the tail needle and inserted and fixed, the centrifugal disc rotates at the low speed, the blood at the upper part of the filter cylinder enters the inner bottom of the filter cylinder through the filter membrane, the centrifugal force born by the piston plate under the low speed is smaller without obvious displacement, and then the centrifugal disc enters the high speed, simultaneously, the driving component drives the control rod to rotate to open the first valve plate and simultaneously close the second valve plate, at the moment, the liquid inlet pipe is opened, the liquid outlet pipe is closed, under the high-speed gear, the centrifugal force born by the piston plate is increased and is larger than the elastic force of the first spring at the bottom of the piston plate, the piston plate starts to move downwards to suck waste liquid in the filter cartridge into the storage cylinder from the liquid inlet pipe, then the centrifugal disc stops working, the driving component simultaneously drives the control rod to rotate to close the first valve plate and simultaneously open the second valve plate, at the moment, the liquid inlet pipe is closed, the liquid outlet pipe is opened, the piston plate resets under the action of the first spring to discharge the waste liquid above the piston plate from the liquid outlet pipe, and when the centrifugal disc stops, the filter cartridge is taken out to unscrew the box cover to take out the filter membrane.
2. The intelligent device for enriching abnormal blood large cells according to claim 1, wherein the driving assembly comprises a first sliding trapezoidal block, the first sliding trapezoidal block moves linearly in the axial direction in the centrifugal hole, a second spring is arranged on the first sliding trapezoidal block, one end of the second spring is fixed on the inner wall of the storage cylinder, the second spring is used for resetting the first sliding trapezoidal block, a first rack is arranged on the first sliding trapezoidal block, a first gear is arranged on the control rod, and the first rack is meshed with the first gear.
3. The intelligent blood abnormal large cell enrichment device according to claim 2, further comprising a blending mechanism for controlling the piston plate to move under the centrifugal action to extract the waste liquid in the filter cartridge.
4. The intelligent device for enriching abnormal blood large cells according to claim 3, wherein the deployment mechanism comprises a first support rod, a second support rod and a second trapezoid block, one end of the first support rod is fixed on the bottom surface of the piston plate, the second support rod is sleeved on the first support rod in a sliding manner, one end of the second support rod, which is far away from the first support rod, is rotationally connected to the inner bottom of the centrifugal hole, a Z-shaped groove is formed in the surface of the second support rod, a protruding portion is arranged at one end of the first support rod, the protruding portion is arranged in the Z-shaped groove, a second spring is arranged in the second support rod, one end of the first support rod is propped against the second spring, a second gear is sleeved on the second support rod, the second trapezoid block is slidably connected to the inner bottom of the centrifugal hole and moves in a linear manner in the radial direction of the centrifugal disc, a second rack is arranged on the second trapezoid block, a third spring is arranged on the second trapezoid block, the third spring is fixed on the inner wall of the second trapezoid block, the second trapezoid block is in a reset mode, and the second trapezoid block is in parallel to the inner side of the second trapezoid block.
5. The intelligent blood abnormal large cell enrichment device according to claim 1, further comprising a box body, wherein the centrifugal disc is arranged in the box body, and a box cover is arranged on the box body and used for covering the centrifugal hole.
6. The intelligent device for enriching abnormal blood large cells according to claim 1, further comprising a cleaning mechanism, wherein the cleaning mechanism is arranged on the centrifugal disc and comprises an electric push rod, a cleaning cavity is formed in the middle of the centrifugal disc and communicated with each through hole of the centrifugal hole, the electric push rod is arranged in the cleaning cavity, a sealing plate is arranged at the output end of the electric push rod, and the sealing plate is connected in the cleaning cavity in a dynamic sealing manner.
7. The intelligent blood-abnormal large cell enrichment device according to claim 1, wherein the inner diameter of the storage cylinder is larger than the inner diameter of the blood container.
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