CN220999659U - Separated blood cell centrifuge tube - Google Patents
Separated blood cell centrifuge tube Download PDFInfo
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- CN220999659U CN220999659U CN202322580805.5U CN202322580805U CN220999659U CN 220999659 U CN220999659 U CN 220999659U CN 202322580805 U CN202322580805 U CN 202322580805U CN 220999659 U CN220999659 U CN 220999659U
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- tube
- inner tube
- groove
- blood cell
- sealing
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- 210000000601 blood cell Anatomy 0.000 title claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 38
- 230000006835 compression Effects 0.000 claims description 10
- 238000007906 compression Methods 0.000 claims description 10
- 238000000926 separation method Methods 0.000 abstract description 8
- 210000004027 cell Anatomy 0.000 description 18
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
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- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The utility model relates to the technical field of blood cell separation and discloses a separated blood cell centrifuge tube, which comprises an inner tube, wherein an outer tube is sleeved outside the inner tube, a diversion hole is formed in the center position of one end of the inner tube in the outer tube, a sealing film is adhered to the diversion hole, sealing covers are jointly connected to the opening ends of the inner tube and the outer tube, a puncture device is arranged on the sealing covers, and the puncture device can puncture the sealing film or block the diversion hole.
Description
Technical Field
The utility model relates to the technical field of blood cell separation, in particular to a separated blood cell centrifuge tube.
Background
Blood cell separation is a special technique for separating and analyzing cells by utilizing centrifugal force according to the differences of sedimentation coefficients, diffusion coefficients and buoyancy densities of different cells. The blood to be separated is stored through the centrifuge tube, the centrifuge is controlled to realize layering on the blood in the centrifuge tube according to different requirements, and then cells of different levels in the centrifuge tube are respectively extracted according to the requirements.
At present, the Chinese patent application number 202320279589.8 discloses a separated blood cell centrifuge tube, which is provided with an inner tube and an outer tube, the bottom of the inner tube is provided with a miniature opening, and blood is pumped and discharged through a piston at the top of the inner tube, so that different layers of cells are conveniently separated, but the blood is layered in the inner tube, the inner tube needs to be ensured to be kept in a sealing state in the centrifuging process, the piston is made of an elastic material, the situation that the piston is unbalanced after blood is pumped exists, and air leakage is easy to occur when the piston rotates at a high speed, so that the blood leaks into the outer tube, and the separation result is influenced.
Disclosure of utility model
Aiming at the defects of the prior art, the utility model aims to provide a separated blood cell centrifuge tube which is convenient for extracting cells at different levels.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
The utility model provides a cellular-type blood cell centrifuging tube, includes the inner tube, the cover is equipped with the outer tube in the inner tube outside, the branching hole has been seted up to the inner tube in the central point of one end in the outer tube, the bonding has the envelope on the branching hole, the inner tube and the open end of outer tube are connected with the closing cap jointly, be provided with puncture device on the closing cap, puncture device can puncture the envelope or block up the branching hole.
The utility model is further provided with: the sealing cover comprises a top plate, the top plate is covered on the openings of the inner pipe and the outer pipe, a connecting column is arranged at one end, deviating from the inner pipe, of the top plate, a mounting groove, a first limiting groove and a second limiting groove are formed at one end, deviating from the top plate, of the connecting column, the mounting groove is in a column shape coaxial with the connecting column and is communicated with the first limiting groove and the second limiting groove, the first limiting groove and the second limiting groove are symmetrically arranged relative to the mounting groove, the first limiting groove and the second limiting groove are evenly distributed along the circumferential direction of the mounting groove, and the groove depth of the first limiting groove is smaller than that of the second limiting groove.
The utility model is further provided with: the puncture device comprises a push rod, the push rod and the inner tube are coaxially arranged, one end of the push rod in the mounting groove is connected with a compression spring, the other end of the compression spring is connected with the bottom of the mounting groove, and the push rod penetrates through the sealing cover and extends into the inner tube.
The utility model is further provided with: the ejector rod comprises a handle, the compression spring is fixed on the handle, the handle and the mounting groove are coaxially arranged, a limiting block is arranged at one end of the handle, facing the top plate, of the handle, the limiting block is symmetrically arranged relative to the handle and can be respectively clamped with the first limiting groove or the second limiting groove, the handle is connected with a puncture needle, the puncture needle points to the diversion hole along the coaxial direction of the inner pipe, and the puncture needle can puncture the sealing film.
The utility model is further provided with: the diversion hole is a conical hole, and the needle point of the puncture needle can be attached to the inner wall of the diversion hole.
The utility model is further provided with: the sealing cover is coaxially provided with a sealing ring, a first internal thread ring and a second internal thread ring, the first internal thread ring and the second internal thread ring are respectively in threaded connection with the inner pipe and the outer pipe, and the sealing ring can be in sealing connection with the inner wall of the inner pipe.
The utility model is further provided with: the outer tube is internally provided with a retaining ring, and the retaining ring is sleeved on the outer wall of the inner tube.
The utility model has the advantages that:
1. The blood is centrifuged in the inner tube, when the puncture device does not puncture the sealing film, the inner tube forms a sealed space, the risk of blood leakage in the centrifuging process is reduced, and meanwhile, each structure on the centrifuge tube is symmetrically designed, so that the centrifuge tube has balance, the shaking generated during high-speed rotation is avoided, and the separation effect of cells is ensured;
2. The limiting block is clamped in the first limiting groove and is pressed towards the bottom of the inner tube under the action of the pressing spring, a certain gap exists between the needle point of the puncturing device and the sealing film, the sealing performance of the inner tube in the centrifuging process is guaranteed, when different-level cells need to be separated, the ejector rod is adjusted, the limiting block is placed in the second limiting groove and is pressed against the bottom of the second limiting groove, at the moment, the needle point punctures the sealing film and is sealed with the diversion hole, the handle is pulled, the needle point leaves the diversion hole, lower-layer cells in the inner tube flow into the outer tube through the diversion hole, the handle is released, the needle point seals the diversion hole, the cells in different levels are separated into the inner tube and the outer tube, the operation is simple, and the extraction of the cells in different levels after centrifuging is convenient.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present utility model;
FIG. 2 is a top view of the device shown in FIG. 1;
FIG. 3 is a cross-sectional view taken along line A-A of FIG. 2;
FIG. 4 is an enlarged view of portion B shown in FIG. 3;
FIG. 5 is a schematic view of the lancing device according to the present utility model;
In the figure: 1. an inner tube; 11. a diversion aperture; 2. an outer tube; 3. a cover; 31. a top plate; 32. a connecting column; 33. an internal thread ring I; 34. an internal thread ring II; 35. a mounting groove; 36. a first limit groove; 37. a limiting groove II; 38. a seal ring; 4. a push rod; 41. a puncture needle; 42. a handle; 43. a limiting block; 5. a compression spring; 6. sealing the film; 7. a retaining ring.
Detailed Description
It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other. The utility model will be described in detail below with reference to the drawings in connection with embodiments.
It is noted that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless otherwise indicated.
In the present utility model, unless otherwise indicated, the terms "upper" and "lower" are used generally with respect to the directions shown in the drawings, or with respect to the vertical, vertical or gravitational directions; also, for ease of understanding and description, "left, right" is generally directed to the left, right as shown in the drawings; "inner and outer" refer to inner and outer relative to the outline of the components themselves, but the above-described orientation terms are not intended to limit the present utility model.
Referring to fig. 1-5, the present utility model provides the following technical solutions:
The utility model provides a cellular-type blood cell centrifuging tube, including inner tube 1, inner tube 1 outside cover is equipped with outer tube 2, division hole 11 has been seted up on the central point of inner tube 1 bottom, seal membrane 6 has been adhered to the bottom of inner tube 1 inner wall, seal membrane 6 lid closes in division hole 11 top, thereby separate inner tube 1 and outer tube 2, the open end of inner tube 1 and outer tube 2 is connected with closing cap 3 jointly, closing cap 3 is fixed and sealed inner tube 1 and outer tube 2, the centrifuging tube is placed into the centrifuge and is carried out the centrifugation operation of being convenient for, be provided with on closing cap 3, before extracting the layering cell that the centrifugation produced, there is certain clearance between piercing device and the seal membrane 6, when needing the reposition of redundant personnel cell liquid, piercing device can puncture seal membrane 6, after the reposition of redundant personnel is accomplished, piercing device can block up division hole 11 and realize the separation of inner tube 1 and outer tube 2.
The sealing cover 3 comprises a top plate 31, the top plate 31 is covered on the openings of the inner pipe 1 and the outer pipe 2, a connecting column 32 is arranged at the upper end of the top plate 31, a mounting groove 35, a first limit groove 36 and a second limit groove 37 are arranged on the upper end surface of the connecting column 32, the mounting groove 35 is in a column shape coaxial with the connecting column 32 and is communicated with the first limit groove 36 and the second limit groove 37, the first limit groove 36 and the second limit groove 37 are symmetrically arranged relative to the mounting groove 35, the first limit groove 36 and the second limit groove 37 are arranged in a 90-degree cross mode, and the groove depth of the first limit groove 36 is smaller than that of the second limit groove 37;
The puncturing device comprises a push rod 4, wherein the push rod 4 and the inner tube 1 are coaxially arranged, one end of the push rod 4 in a mounting groove 35 is connected with a compression spring 5, the other end of the compression spring 5 is connected with the bottom of the mounting groove 35, the compression spring 5 enables the push rod 4 to be always attached to the bottom of a first limit groove 36 or a second limit groove 37, and the push rod 4 penetrates through a sealing cover 3 and extends into the inner tube 1;
The ejector rod 4 comprises a handle 42, the compression spring 5 is fixed on the handle 42, the handle 42 and the mounting groove 35 are coaxially arranged, a limiting block 43 is arranged at one end of the handle 42 facing the top plate 31, the limiting block 43 is symmetrically arranged relative to the handle 42 and can be respectively clamped with the first limiting groove 36 or the second limiting groove 37, the handle 42 is connected with a puncture needle 41, the puncture needle 41 points to the diversion hole 11 along the coaxial direction of the inner tube 1, and a certain gap exists between the puncture needle 41 and the sealing film 6 when the limiting block 43 is positioned in the first limiting groove 36; when the limiting block 43 is positioned in the limiting groove II 37, the puncture needle 41 punctures the sealing film 6.
The diversion hole 11 is a conical hole, and the needle tip of the puncture needle 41 can be attached to the inner wall of the hole of the diversion hole 11, so that the puncture needle 41 can seal the diversion hole 11, and separate the inner tube 1 from the outer tube 2 after the cells of different levels are diverted.
The sealing cover 3 is coaxially provided with a sealing ring 38, a first internal thread ring 33 and a second internal thread ring 34, the first internal thread ring 33 and the second internal thread ring 34 are respectively in threaded connection with the inner pipe 1 and the outer pipe 2, and the sealing ring 38 can be in sealed connection with the inner wall of the inner pipe 1; thereby fixing and sealing the positions of the inner tube 1 and the outer tube 2, and facilitating the subsequent centrifugation operation.
The outer tube 2 is internally provided with the retaining ring 7, and the retaining ring 7 is sleeved on the outer wall of the inner tube 1, so that additional support and limitation are provided for the inner tube 1, the rotation stability during centrifugal operation is improved, and the separation effect is ensured.
Specifically, the blood is centrifuged in the inner tube 1, when the puncture device does not puncture the sealing film 6, the inner tube 1 forms a sealed space, the risk of blood leakage in the centrifuging process is reduced, and meanwhile, each structure on the centrifuge tube is symmetrically designed, so that the centrifuge tube has balance, the shaking generated during high-speed rotation is avoided, and the separation effect of cells is ensured;
The limiting block 43 is clamped in the limiting groove I36 and is pressed towards the bottom of the inner tube 1 under the action of the pressing spring 5, a certain gap exists between the needle point of the puncturing device and the sealing film 6, the tightness of the inner tube 1 in the centrifuging process is guaranteed, when different-level cells are required to be separated, the ejector rod 4 is adjusted, the limiting block 43 is placed in the limiting groove II 37 and is pressed against the bottom of the limiting groove II 37, at the moment, the needle point punctures the sealing film 6 and is sealed with the diversion hole 11, the handle 42 is pulled, the needle point leaves the diversion hole 11, lower-layer cells in the inner tube 1 flow into the outer tube 2 through the diversion hole 11, the handle 42 is released, the needle point seals the diversion hole 11, the cells in different levels are placed in the inner tube and the outer tube, the operation is simple, and the extraction of the cells in different levels after centrifuging is convenient.
It will be apparent that the embodiments described above are merely some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present utility model without making any inventive effort, shall fall within the scope of the present utility model.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the application described herein may be implemented in sequences other than those illustrated or otherwise described herein.
The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (7)
1. A divided blood cell centrifuge tube comprising an inner tube (1), characterized in that: the novel plastic sealing device is characterized in that an outer tube (2) is sleeved outside the inner tube (1), a diversion hole (11) is formed in the center of one end of the inner tube (1) in the outer tube (2), a sealing film (6) is adhered to the diversion hole (11), sealing covers (3) are jointly connected to the opening ends of the inner tube (1) and the outer tube (2), a puncturing device is arranged on the sealing covers (3), and the puncturing device can puncture the sealing film (6) or block the diversion hole (11).
2. A divided blood cell centrifuge tube as recited in claim 1 wherein: the sealing cover (3) comprises a top plate (31), the top plate (31) is covered on the opening of the inner pipe (1) and the outer pipe (2), one end of the top plate (31) deviating from the inner pipe (1) is provided with a connecting column (32), one end of the connecting column (32) deviating from the top plate (31) is provided with a mounting groove (35), a first limit groove (36) and a second limit groove (37), the mounting groove (35) is in a column shape coaxial with the connecting column (32) and is communicated with the first limit groove (36) and the second limit groove (37), the first limit groove (36) and the second limit groove (37) are symmetrically arranged relative to the mounting groove (35), the first limit groove (36) and the second limit groove (37) are evenly distributed along the circumferential direction of the mounting groove (35), and the groove depth of the first limit groove (36) is smaller than that of the second limit groove (37).
3. A divided blood cell centrifuge tube as recited in claim 2 wherein: the puncture device comprises a push rod (4), the push rod (4) and the inner tube (1) are coaxially arranged, one end of the push rod (4) in the mounting groove (35) is connected with a compression spring (5), the other end of the compression spring (5) is connected with the groove bottom of the mounting groove (35), and the push rod (4) penetrates through the sealing cover (3) and extends into the inner tube (1).
4. A divided blood cell centrifuge tube according to claim 3, wherein: ejector pin (4) include handle (42), hold-down spring (5) are fixed on handle (42), handle (42) and mounting groove (35) coaxial setting, handle (42) are provided with stopper (43) towards the one end of roof (31), stopper (43) set up about handle (42) symmetry to can be respectively with spacing groove one (36) or spacing groove two (37) looks block, be connected with puncture needle (41) on handle (42), puncture needle (41) are along directional branch flow hole (11) of inner tube (1) coaxial direction, puncture needle (41) can puncture envelope (6).
5. A divided blood cell centrifuge tube as recited in claim 4 wherein: the diversion hole (11) is a conical hole, and the needle point of the puncture needle (41) can be attached to the inner wall of the diversion hole (11).
6. A divided blood cell centrifuge tube as recited in claim 1 wherein: the sealing cover (3) is coaxially provided with a sealing ring (38), a first internal thread ring (33) and a second internal thread ring (34), the first internal thread ring (33) and the second internal thread ring (34) are respectively in threaded connection with the inner pipe (1) and the outer pipe (2), and the sealing ring (38) can be in sealed connection with the inner wall of the inner pipe (1).
7. A divided blood cell centrifuge tube as recited in claim 1 wherein: the inner tube (2) is internally provided with a retaining ring (7), and the retaining ring (7) is sleeved on the outer wall of the inner tube (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322580805.5U CN220999659U (en) | 2023-09-22 | 2023-09-22 | Separated blood cell centrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322580805.5U CN220999659U (en) | 2023-09-22 | 2023-09-22 | Separated blood cell centrifuge tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220999659U true CN220999659U (en) | 2024-05-24 |
Family
ID=91115082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322580805.5U Active CN220999659U (en) | 2023-09-22 | 2023-09-22 | Separated blood cell centrifuge tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220999659U (en) |
-
2023
- 2023-09-22 CN CN202322580805.5U patent/CN220999659U/en active Active
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