CN214937438U

CN214937438U - Blood cell separating tube

Info

Publication number: CN214937438U
Application number: CN202121351098.7U
Authority: CN
Inventors: 李勇; 谢海涛; 薛卫巍; 苏道庆
Original assignee: Dongguan Zailijian Biotechnology Co ltd
Current assignee: Dongguan Zailijian Biotechnology Co ltd
Priority date: 2021-06-17
Filing date: 2021-06-17
Publication date: 2021-11-30
Anticipated expiration: 2031-06-17

Abstract

The utility model discloses a blood cell separation tube, which comprises a tube body and a tube cover; a flow guide cavity is arranged on the side wall of the pipe body, a flow guide through is formed between the flow guide cavity and the pipe cavity of the pipe body, and the depth of the flow guide cavity is the same as that of the pipe cavity; the pipe cover is sealed to cover the opening end of the pipe cavity and the opening end of the flow guide cavity. After the blood is filled into the tube body and centrifuged, the separating tube arranges the upper plasma layer in the tube cavity of the tube body by the principle of a communicating vessel, arranges the lower red blood cell layer in the flow guide cavity, pours the red blood cell layer out of the flow guide cavity, and reserves the tube cavity and the flow guide cavity for later use; the design of the diversion cavity can easily and cleanly remove the lower red blood cell layer obtained after blood centrifugation, and the upper blood plasma layer can be reserved and does not contain red blood cells.

Description

Blood cell separating tube

Technical Field

The utility model relates to a blood cell separation preparation facilities especially relates to a blood cell separation tube.

Background

Centrifugation of blood components is a commonly used procedure in medical testing and in human cell separation. The blood is centrifuged, generally by injecting the blood into a centrifuge tube, rotating the centrifuge tube at a high speed in a centrifuge to stratify the blood components, and then extracting the required stratified fluid. In the blood cell layer extraction experiment, the supernatant, i.e., the plasma layer (including cells and platelets), after the centrifugation treatment is taken, and the lower layer, i.e., the red blood cell layer, is removed.

The upper liquid is taken after the separation of the existing centrifugal tube, and the mode of extracting by an injector and obliquely pouring out by the centrifugal tube is generally adopted. The former method is complicated to operate; in the latter mode, the lower layer liquid, namely the red blood cells, is easy to pour out in the pouring process, so that red blood cell impurities are mixed in the upper layer liquid.

SUMMERY OF THE UTILITY MODEL

Based on the above problem, the utility model aims to solve the problem that a blood cell separation tube that blood passes through after the centrifugation, can get rid of red blood cell layer and remain the plasma layer through simple operation mode is provided.

The technical scheme of the utility model as follows:

a blood cell separation tube comprises a tube body and a tube cover; a flow guide cavity is arranged on the side wall of the pipe body, a flow guide through is formed between the flow guide cavity and the pipe cavity of the pipe body, and the depth of the flow guide cavity is the same as that of the pipe cavity; the pipe cover is sealed and covers the opening end of the pipe cavity and the opening end of the flow guide cavity.

In one embodiment, the blood cell separation tube has a plurality of flow guide holes formed in a lower sidewall of the tube body, and the flow guide holes connect the tube cavity and the flow guide cavity in a penetrating manner.

In one embodiment, the blood cell separation tube is further provided with a transverse partition board in the tube cavity, and the transverse partition board divides the tube cavity into an upper tube cavity and a lower tube cavity; the transverse partition plate is provided with micropores, and the micropores enable the upper tube cavity and the lower tube cavity to be communicated in a one-way mode from top to bottom.

The utility model provides a blood cell separator tube, after the blood centrifugation of packing into, this separator tube then through the linker principle, arrange the upper plasma layer in the lumen of body, and the water conservancy diversion chamber is then arranged in to lower floor's red blood cell layer, then pours out the red blood cell layer from the water conservancy diversion chamber, and the upper plasma layer then remains the lumen and in the water conservancy diversion chamber, leaves reserve. The design of the flow guide cavity can easily and cleanly remove the lower red blood cell layer obtained after blood centrifugation, and the upper blood plasma layer can be reserved and does not contain red blood cells; meanwhile, the flow guide cavity can also discharge air in the lymph separation liquid to the outside when the lymph separation liquid is injected, so that the lymph separation liquid is conveniently injected.

Drawings

Fig. 1 is a schematic view of a blood cell separation tube structure according to an embodiment of the present invention;

FIG. 2 is a schematic view of a blood cell separation tube according to another embodiment of the present invention;

FIG. 3 is a schematic diagram of the structure of the blood cell separation tube of FIG. 2 before centrifugation;

FIG. 4 is a schematic diagram of the structure of the blood cell separation tube corresponding to FIG. 3 after centrifugation;

FIG. 5 is a schematic view of a closed state of the one-way conduction valve;

FIG. 6 is a schematic view of the open state of the one-way conduction valve.

Detailed Description

The following describes the preferred embodiments of the present invention in further detail with reference to the accompanying drawings.

As shown in fig. 1 to 4, the present invention provides a blood cell separation tube 100, which comprises a tube body 120 and a tube cap 110. The outer shape of the tube body 120 is formed by combining an upper cylinder 1202 and a lower cone 1201. The tube body 120 and the tube cover 110 are generally made of medical PVC (polyvinyl chloride), PC (polycarbonate), glass, or the like.

In the blood cell separation tube 100, a flow guide cavity 102 is provided on a side wall of a tube body 121, and a flow guide penetration including an air flow and a liquid flow is formed between the flow guide cavity 102 and a tube cavity 101 of a tube body 120. A plurality of flow guiding holes 1231 are formed in the lower sidewall of the tube body 120, that is, the lower cone 1021, and the flow guiding holes 1231 connect the tube cavity 101 and the flow guiding cavity 102 in a penetrating manner. The diversion cavity 102 is a cavity formed by the diversion shell 121 and the outer wall of the tube body 120, and the volume of the diversion cavity 102 is about 30-50% of the volume of the tube cavity 101, which is beneficial to separating and removing the lower layer red blood cell layer 131 after the blood 130 in the blood cell separation tube 100 is centrifuged. The diversion hole 1231 is disposed in the lower cone 1201 of the tube 121 and located at the joint of the lumen 101 and the diversion cavity 102.

Preferably, the diameter of the flow guide hole 1231 is 5-10 micrometers; thus, during the blood centrifugation process, the red blood cells can pass through the blood centrifugation process.

Preferably, the depth of the diversion cavity 102 is the same as the depth of the lumen 101, that is, the open end 1021 of the diversion cavity 102 is flush with the open end 1011 of the lumen 101; thus, the cap 110 can fit over the open end 1011 of the sealed cap lumen 101 and the open end 1021 of the diversion lumen 102.

In another embodiment, as shown in fig. 2 to 4. In the blood cell separation tube 100, after the blood 130 is centrifuged, in order to effectively distinguish and isolate the upper plasma layer 132 (including plasma, monocyte, leukocyte, platelet, and density solution, etc.) and the lower red blood cell layer 131 (including erythrocyte, granulocyte density solution, etc.), a transverse partition plate 122 needs to be disposed in the lumen 101, the transverse partition plate 122 divides the lumen 101 into an upper lumen 104 and a lower lumen 103, and the transverse partition plate 122 is provided with micropores 1221, the pore size of which is substantially similar to the particle size of the red blood cells, so as to facilitate the passage of the erythrocytes. The micropores 1221 form a one-way conduction channel from top to bottom between the upper tube cavity 104 and the lower tube cavity 103, that is, after the blood 130 is centrifuged, the plasma layer 132 in the upper tube cavity 104 cannot flow to the lower tube cavity 103 through the micropores 1221 of the transverse partition 122 by its own gravity; thus, when the lower red blood cell layer 131 is separated and removed, the plasma layer 132 can be kept in the upper lumen 104 cleanly, the blood cell separation effect is greatly improved, and the operation is simply realized by directly pouring out the red blood cell layer.

Preferably, in order to facilitate the flow of blood components and avoid the blood components from remaining on the wall of the lumen 101, an included angle of 120-150 degrees is formed between the transverse partition plate 122 and the wall of the lumen 101, and the middle part is inclined towards the lower lumen, so as to ensure that no blood components remain at the included angle.

Preferably, as shown in fig. 2 to 6, a liquid-guiding one-way conduction valve 140 is disposed at the micro-hole 1221, the one-way conduction valve 140 includes a plurality of elastic valve plates 141, one end of each valve plate 141, that is, a fixed end 1411, is disposed on the bottom surface of the transverse partition 122 around the micro-hole 1221, and the other end of each valve plate 141, that is, a hanging end 1412, is overlapped with each other in a hanging manner to form a funnel-like structure, so as to achieve the effect of sealing the micro-hole 1221, and the hanging ends 1412 of each valve plate 141 are separated from each other under the centrifugal force to form a through-hole 1413, so that the micro-hole 1221 is conducted, that is, the micro-hole 1221 forms a one-way conduction channel from the upper end a to the lower end b between the upper tube cavity 104 and the lower tube cavity 103; after the centrifugal force is removed, the hanging end 1412 of each valve plate 141 is restored to the overlapped state by virtue of the elasticity of the hanging end 1412, and the micropores 1221 are closed; thus, the valve plate 141 of the one-way conduction valve 140 makes the micro-hole 1221 in a conduction or closing state under the action of the external centrifugal force and the self-elastic force. Wherein, the elastic force of each valve plate 141 is much larger than the weight of the blood 130, if the hanging end of the valve plate 141 can not be opened only by the weight of the blood in the separation tube 100, the centrifuged upper layer plasma layer 132 can not flow into the lower layer red blood cell layer 131, so as to ensure that the red blood cells can not be mixed in the plasma layer 132.

Taking the blood cell separation tube 100 shown in fig. 2 to 4 as an example, the flow of use is as follows:

1. firstly, a lymph separation liquid and/or an anticoagulant is injected into a lower tube cavity 103 of a tube body 120 of the separation tube 100 through an opening end 1011 of the tube cavity 101, and air in the lower tube cavity 103 is discharged through a diversion cavity 102 and an opening end 1021 of the diversion cavity 102 in sequence;

2. then, the blood 130 is injected into the upper lumen 104 of the tube body 120 of the separation tube 100 through the open end 1011 of the lumen 101; as shown in fig. 3;

3. after the tube cap 110 is sealed and covered, the separation tube 100 is placed in a centrifuge for centrifugation according to the conventional operation of blood cell centrifugation; at this time, the blood 130 is centrifuged into an upper plasma layer (including monocytes, platelets, leukocytes, etc.) 132 and a lower red blood cell layer 131; as shown in fig. 4;

4. finally, the upper plasma layer (including monocytes, platelets, leukocytes, etc.) 132 is decanted for use, and the lower red blood cell layer is removed.

It should be understood that the above description of the preferred embodiments of the present invention is given in some detail and should not be taken as limiting the scope of the invention, which is defined by the appended claims.

Claims

1. A blood cell separation tube is characterized by comprising a tube body and a tube cover; a flow guide cavity is arranged on the side wall of the pipe body, a flow guide through is formed between the flow guide cavity and the pipe cavity of the pipe body, and the depth of the flow guide cavity is the same as that of the pipe cavity; the pipe cover is sealed and covers the opening end of the pipe cavity and the opening end of the flow guide cavity.

2. The blood cell separation tube of claim 1, wherein a plurality of flow guiding holes are formed in a lower side wall of the tube body, and the flow guiding holes connect the tube cavity and the flow guiding cavity in a penetrating manner.

3. The blood cell separation tube of claim 2, wherein the diameter of the flow guide hole is 5 to 10 μm.

4. The blood cell separation tube according to claim 1, wherein a transverse partition plate is further provided in the lumen, the transverse partition plate dividing the lumen into an upper lumen and a lower lumen; the transverse partition plate is provided with micropores, and the micropores enable the upper tube cavity and the lower tube cavity to be communicated in a one-way mode from top to bottom.

5. The blood cell separation tube of claim 4, wherein a one-way conduction valve for guiding liquid is disposed at the micro-hole, the one-way conduction valve comprises a plurality of elastic valve plates, one end of each valve plate is fixedly disposed on the bottom surface of the transverse partition plate around the micro-hole, the other end of each valve plate is overlapped with each other in a hanging manner to seal the micro-hole, and the hanging ends of each valve plate are separated from each other under the action of an external centrifugal force to conduct the micro-hole; and after the centrifugal force is removed, the suspension end of each valve plate is restored to an overlapped state by virtue of the elasticity of the suspension end, and the micropores are closed.