CN212051345U

CN212051345U - Centrifugal circulation tumor cell step-by-step separation device

Info

Publication number: CN212051345U
Application number: CN202020252359.9U
Authority: CN
Inventors: 王振丹; 郝继辉; 李胜; 李�浩; 崔凯
Original assignee: Shandong First Medical University and Shandong Academy of Medical Sciences
Current assignee: Shandong Kaige Intelligent Machine Co ltd
Priority date: 2020-03-04
Filing date: 2020-03-04
Publication date: 2020-12-01
Anticipated expiration: 2030-03-04

Abstract

The to-be-solved problem of the utility model is to provide a centrifugal circulation tumor cell separator step by step can separate circulation tumor cell and collect step by step according to the size of a dimension. The rotary body is in rotary fit with the inside of the shell; a plurality of vertically distributed grooves I are processed on the outer circumferential wall of the rotating body, and a plurality of vertically distributed grooves II are processed on the inner circumferential wall of the shell; the upper ends of the shell and the rotating body are liquid inlets, and the lower ends of the shell and the rotating body are liquid outlets.

Description

Centrifugal circulation tumor cell step-by-step separation device

Technical Field

The utility model relates to a circulation tumor cell detection area, concretely relates to centrifugal circulation tumor cell is separator step by step

Background

Circulating tumor cells generally refer to cells that are derived from primary tumor tissue, are exfoliated and stained into peripheral blood of a human body, can colonize different tissues during the process of circulating cell metastasis, and can be found on a plurality of solid-phase tumors after metastasis, so that the circulating tumor cells can be used as an important marker for early tumor diagnosis. In addition, circulating tumor cells play an important role in the metastasis and recurrence of tumors. Therefore, the method has very important significance for the research of the physiological mechanism of the circulating tumor cells. However, the number of circulating tumor cells and their absolute concentration in human peripheral blood is very low, making the study of circulating tumor cells very difficult. Sorting and capturing of circulating tumor cells has therefore become an important issue of research.

The detection of CTCs is to separate tumor cells from blood cells in blood, wherein one separation method is to separate and enrich CTCs and blood cells according to their physical characteristics. For example, in the microfluidic cell capture chip in the prior art, a human body fluid sample passes through the microfluidic cell capture chip, so as to collect circulating tumor cells with a specific size, and then perform the next experiment. The size of circulating tumor cells is also different, and if the circulating tumor cells can be collected separately for research and attack, the circulating tumor cells play an important role in inhibiting the clinical application of CTCs. However, the existing microfluidic cell capture chip does not have the function of gradually separating tumor cells.

SUMMERY OF THE UTILITY MODEL

The to-be-solved problem of the utility model is to provide a centrifugal circulation tumor cell separator step by step can separate circulation tumor cell and collect step by step according to the size of a dimension.

The utility model discloses a realize through following technical scheme:

a centrifugal type circulating tumor cell step-by-step separation device comprises a shell and a rotating body, wherein the rotating body is matched inside the shell in a rotary mode;

a plurality of vertically distributed grooves I are processed on the outer circumferential wall of the rotating body, and a plurality of vertically distributed grooves II are processed on the inner circumferential wall of the shell;

the upper ends of the shell and the rotating body are liquid inlets, and the lower ends of the shell and the rotating body are liquid outlets.

Further, the shell is in a cylindrical shape with an opening at the upper end and a closed lower end; the outer side wall of the shell close to the bottom end is provided with three strip-shaped outlets, and the bottom of the shell is provided with a rotating device capable of synchronously rotating the shell and the rotating body; the grooves I and the grooves II are uniformly distributed at intervals.

Further, rotary device includes base and driving motor, driving motor installs the downside of base, driving motor's output shaft vertically upwards passes the base and with the bottom of casing can be dismantled and be connected.

Furthermore, the cross section of an output shaft of the driving motor is square, and a square hole which can be matched and spliced with the driving motor is processed on the bottom end of the shell.

Further, a liquid inlet hopper is installed at the upper end of the shell, and the rotating speed of the shell is 60-70 r/min.

Furthermore, the number of the grooves I and the grooves II is 12-36.

Compared with the prior art, the utility model the beneficial effect who gains as follows:

1. by adjusting the rotating body to rotate and move relative to the shell, the groove I and the groove II move relative to each other to form cell fluid channels with different sizes, circulating tumor cells with different sizes in the range of 10-30um can be sorted and passed through, so that the circulating tumor cells can be sorted step by step, and the research on the circulating tumor cells is facilitated;

2. the design of the rotating device can ensure that the sorted circulating tumor cells are quickly collected into a collecting vessel under the centrifugal action, thereby greatly improving the working efficiency;

3. the utility model discloses simple structure, low in manufacturing cost, convenient operation are used.

Drawings

Fig. 1 is a schematic perspective view of a step-by-step separation device according to the present invention;

FIG. 2 is a front view of the step-by-step separation device of the present invention;

fig. 3 is a schematic view of the housing and the rotating body according to the present invention;

fig. 4 is a top view of the housing and the rotating body of the present invention;

fig. 5 is a schematic perspective view of the collecting vessel according to the present invention;

in the figure: 1. casing, 2, rotator, 3, base, 4, driving motor, 5, feed liquor fill, 6, recess I, 7, recess II, 8, bar export, 9, collection household utensils.

Detailed Description

The utility model provides a centrifugal circulation tumor cell separator step by step mainly includes columniform casing 1, columniform rotator 2, base 3 and driving motor 4. The upper end of the shell 1 is an opening, the rotating body 2 is installed in the shell 1 in a rotating and matching mode, 12 vertically-distributed grooves I6 are processed on the outer circumferential wall of the rotating body 2, 12 vertically-distributed grooves II 7 are processed on the inner circumferential wall of the shell 1, and the grooves I6 and the grooves II 7 form a cell fluid channel together. The top processing of rotator 2 has the knob, controls the relative casing 1 rotation of rotator 2 through rotating the knob, and the contact ratio of control recess I6 and recess II 7 to adjust cell fluid passage's size, realize the not unidimensional cell of filtration of selectivity, processing has three bar export 8 on casing 1 is close to the lateral wall of bottom, makes things convenient for the body fluid to flow out. The upper end of the shell 1 is provided with a liquid inlet hopper 5, so that a human body liquid sample can be poured into the shell 1 conveniently.

Base 3's downside installation driving motor 4, driving motor 4's the vertical upwards base 3 that passes of output shaft, driving motor 4's output shaft cross section is square, and processing has the quad slit on casing 1's the bottom, places casing 1 on base 3 like this, through the output shaft cooperation installation of quad slit and driving motor 4, just can realize that driving motor 4 drives casing 1 and rotates with 60 r/min's speed. Thus, the filtered cells are separated through the strip-shaped outlet 8 by the centrifugal force of rotation.

In order to collect cells conveniently, an annular collecting vessel 9 is arranged between the shell 1 and the base 3 in a matching way, the outer circular wall of the collecting vessel 9 is higher than the inner circular wall, and liquid with cells separated from the strip-shaped outlet 8 under the action of centrifugal force falls into the collecting vessel 9.

During the processing, when the groove I and the groove II are completely misaligned, cells with the diameter smaller than 10um can only flow through the cell fluid channel, and most cells in the size range are blood cells. The grooves I and II are half-coincident and can enable cells with the diameter of 10-20um to flow through the cell fluid channel, and the cells in the size range are mostly small-sized circulating tumor cells. The grooves I and II are completely overlapped, so that cells with the diameter of 20-30um can flow through the cell fluid channel, and the cells with the size range are mostly large-sized circulating tumor cells.

Take the tumor cells within the range of 10-20um and 20-30um to be separated as an example, the centrifugal type circulating tumor cell step-by-step separation device has the following specific using method:

adjusting the rotating body to ensure that the groove I and the groove II are not overlapped completely, pouring human body liquid from the liquid inlet hopper, wherein cells with the inner diameter of less than 10 microns in the liquid flow to the strip-shaped outlet through the groove I and the groove II due to the limitation of a fluid space, and enter a collecting vessel under the action of centrifugal force of a driving motor;

replacing the collection vessel, adjusting the rotating body to enable the groove I and the groove II to be overlapped by half, thus ensuring that tumor cells in the range of 10-20um smoothly flow to the strip-shaped outlet through the cell fluid channel and enter the collection vessel under the action of the centrifugal force of the driving motor;

replacing the collection vessel, adjusting the rotating body to enable the grooves I and II to be complete, so that tumor cells in a range of 10-20um can be ensured to smoothly flow to the strip-shaped outlet through the cell fluid channel and enter the collection vessel under the action of centrifugal force of the driving motor;

by the method, tumor cells in the range of 10-20um and tumor cells in the range of 20-30um can be respectively separated and collected for special research.

Claims

1. A centrifugal type circulating tumor cell progressive separation device is characterized by comprising a shell (1) and a rotating body (2), wherein the rotating body (2) is in rotary fit with the inside of the shell (1);

a plurality of vertically distributed grooves I (6) are processed on the outer circumferential wall of the rotating body (2), and a plurality of vertically distributed grooves II (7) are processed on the inner circumferential wall of the shell (1);

the upper ends of the shell (1) and the rotating body (2) are liquid inlets, and the lower ends of the shell and the rotating body are liquid outlets.

2. Progressive separation device according to claim 1, wherein the casing (1) is cylindrical with an open upper end and a closed lower end; the outer side wall of the shell (1) close to the bottom end is provided with three strip-shaped outlets (8), and the bottom of the shell (1) is provided with a rotating device capable of synchronously rotating the shell (1) and the rotating body (2); the grooves I (6) and the grooves II (7) are uniformly distributed at intervals.

3. Progressive separation device according to claim 2, wherein the rotation means comprise a base (3) and a drive motor (4), the drive motor (4) being mounted on the underside of the base (3), the output shaft of the drive motor (4) passing vertically upwards through the base (3) and being detachably connected to the bottom of the housing (1).

4. The progressive separation device according to claim 3, wherein the cross section of the output shaft of the driving motor (4) is square, and a square hole which can be matched and inserted with the driving motor (4) is formed in the bottom end of the shell (1).

5. Progressive separation device according to claim 2, wherein the upper end of the casing (1) is provided with a liquid inlet hopper (5), and the rotation speed of the casing (1) is 60-70 r/min.

6. A progressive separation device according to any one of claims 1 to 5 wherein the number of grooves I (6) and II (7) is from 12 to 36.