CN208087619U - A kind of cell collection preservation separating pipe - Google Patents
A kind of cell collection preservation separating pipe Download PDFInfo
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- CN208087619U CN208087619U CN201820341593.1U CN201820341593U CN208087619U CN 208087619 U CN208087619 U CN 208087619U CN 201820341593 U CN201820341593 U CN 201820341593U CN 208087619 U CN208087619 U CN 208087619U
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- separating pipe
- cell
- separating
- separation
- pipe
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- 238000004321 preservation Methods 0.000 title claims description 18
- 238000000926 separation method Methods 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims description 22
- 239000012780 transparent material Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000002372 labelling Methods 0.000 claims description 3
- 239000003550 marker Substances 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 abstract description 18
- 239000008280 blood Substances 0.000 abstract description 18
- 238000002659 cell therapy Methods 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 7
- 238000002203 pretreatment Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 34
- 238000005070 sampling Methods 0.000 description 4
- 210000001744 T-lymphocyte Anatomy 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000032258 transport Effects 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
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- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000004656 cell transport Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
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- 238000002347 injection Methods 0.000 description 1
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- 230000037431 insertion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
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- 239000002699 waste material Substances 0.000 description 1
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- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The utility model proposes a kind of cell collections to preserve separating pipe, separating pipe lid including separating pipe and with the cooperation of separating pipe nozzle, integrally formed separation neck is equipped in the middle part of the separating pipe, and the internal diameter for detaching neck is respectively smaller than the internal diameter of the separating pipe of separation neck upper and lower part.The utility model solves cumbersome process in " cell therapy " pre-treatment work, realizes blood collection, the standardisation requirements that transport, separation, collection are integrated.
Description
Technical field
The utility model is related to a kind of cell collections to preserve separating pipe.
Background technology
Cell therapy refers to the characteristic using certain cells with specific function, using biological engineering method acquisition and/or
After the processing such as amplification in vitro, specific culture, so that these cells is had, enhancing is immune, kills pathogen and tumour cell, rush
Into the therapeutic efficiencies such as tissue and organ regeneration and physical recovery, to achieve the purpose that treat disease.
For example, CAR-T, Chimeric antigen receptor T cell, are one of the therapeutic modalities of current more effective malignant tumour.
Similar with other immunotherapies, its basic principle is not exactly to remove cancer cell using the immunocyte of patient itself, but not
With this is a kind of cell therapy.
One typical CAR-T treats flow, is broadly divided into following five steps：1. detaching：Separating immune T cell.②
Modification：Activate T cell.3. expanding：In vitro culture.4. feeding back：Cell is fed back.5. monitoring：Guard patient.
Acquisition before cell separation is poured into advance usually using blood taking bag, then by the cell in blood taking bag added with separation respectively
It is centrifuged, collects in the centrifuge tube of liquid.In this all too many levels slightly not carefully will contamination of cells, cause entire
Cell sample cancels, and less serious case influences the health of patient, and severe one causes death.
Utility model content
Facilitating blood sampling, convenience in transport, the cell for facilitating preservation, centrifugation being facilitated to be integrated the object of the present invention is to provide a kind of
Acquisition preserves separating pipe, and it is small to have the characteristics that convenient transportation, easy to operate and sample pollute.
What the technical solution of the utility model was realized in：
A kind of cell collection preservation separating pipe, including separating pipe 2 and the separating pipe lid 1 with the cooperation of 2 nozzle of separating pipe, institute
State the middle part of separating pipe 2 and be equipped with integrally formed separation neck 3, and the internal diameter for detaching neck 3 be respectively smaller than separation 3 top of neck with
The internal diameter of the separating pipe of lower part.
Preferably, the separating pipe of 3 upper and lower part of the separation neck is cylindrical shape, and the internal diameter of the cylinder on top is small
In the internal diameter of the cylinder of lower part.
Preferably, 2 bottom of the separating pipe is oval bottom, is transparent material, convenient for observation centrifugal effect.
Preferably, the separating pipe lid 1 is rubber material, is sealed in 2 nozzle of separating pipe, for keeping negative in separating pipe
Pressure.
Preferably, the separating pipe 2 is equipped with label labeling 5.
Preferably, the separating pipe 2 is transparent material and pipe shaft is equipped with scale marker 4.
Preferably, the cell-preservation liquid 6, separation gel 7, separating liquid 8 of separation preservation are preinstalled in the separating pipe 2.
What the utility model generated has the beneficial effect that：The utility model by the sample collection bag of " cell therapy " pre-treatment,
Centrifuge tube is designed as quantitative collection and preserves isolation integral design, and karyocyte is gathered in the middle part of separating pipe after cell collection separation
Separation tube neck, more effectively collect karyocyte (tunica albuginea layer).By blood taking needle bearing in separating pipe is preserved using cell
Pressure, quantitative collection patient blood are directly entered cell and preserve in separating pipe, and cell is mixed with the preservation liquid in pipe, preserve liquid and protect
Indeformable cell, not atrophy, undamaged feature are protected, cell preservation, cell transport and cell separation are carried out.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, other drawings may also be obtained based on these drawings.
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the structural schematic diagram after the utility model separating pipe lid is opened.
Fig. 3 is schematic diagram after separating pipe collection of specimens of the present invention.
Fig. 4 is design sketch after separating pipe sample of the present invention separation.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
A kind of cell collection preserves separating pipe as shown in Figs. 1-2, including separating pipe 2 and with the cooperation of 2 nozzle of separating pipe
Separating pipe lid 1, the middle part of the separating pipe 2 are equipped with integrally formed separation neck 3, and the internal diameter of separation neck 3 is respectively smaller than point
The internal diameter of separating pipe from 3 upper and lower part of neck.The separating pipe of 3 upper and lower part of neck is detached as cylindrical shape, and top
Cylinder internal diameter be less than lower part cylinder internal diameter；2 bottom of separating pipe is oval bottom, is transparent material, convenient for observation from
Heart effect；The separating pipe lid 1 is rubber material, 2 nozzle of separating pipe is sealed in, for keeping the negative pressure in separating pipe；It is described
Separating pipe 2 is equipped with label labeling 5；The separating pipe 2 is transparent material and pipe shaft is equipped with scale marker 4；The separating pipe 2
Inside it is preinstalled with the cell-preservation liquid 6, separation gel 7, separating liquid 8 of separation preservation.Reagent, cell-preservation liquid 6 are preinstalled in separating pipe
The preservation liquid being added to before upper layer, sampling in separating pipe, it and the sample acquired can react, and protection cell is complete
It is whole, it is not damaged, it does not solidify；Separation gel 7 is in middle level, and the separation gel being added to before sampling in separating pipe is prevented in transportational process
Only cell is mixed with separating liquid；Separating liquid 8 is in lower layer, for the separating liquid being added to before sampling in separating pipe.
Separating pipe design can coordinate existing refrigerated centrifuge use on the market in this programme, be not required to separately develop centrifugation again
Machine avoids waste of resource configuration to greatest extent.Separating pipe lid 1 is that rubber material is flexible, nontoxic, separation lid when collecting sample
It is not required to uncap, blood taking needle Direct perforantes can avoid cell contamination into separating pipe.
As shown in Figure 3-4, separating pipe uses vacuum mode, cooperation blood taking needle directly to acquire patient blood and enter Guan Zhongyu cells
Mixing is preserved, the separation gel 7 in pipe separates the blood of acquisition and separating liquid 8.Cell is layered after centrifugation by proportion,
First layer is blood plasma, in the upper part of caliber；The second layer is karyocyte (tunica albuginea), in separation tube neck aggregation；Third layer is
Separating liquid；4th layer is separation gel；Layer 5 is red blood cell.Karyocyte (tunica albuginea) present invention is collected according to technique to solve
Cumbersome process in " cell therapy " pre-treatment work realizes blood collection, the standardization that transport, separation, collection are integrated
It is required that.
Example：The pre-treatment of patient's blood cell treatment.
Cell therapy is to introduce new cell into a tissue to treat the process of disease.Autogenous cell is taken out, is passed through
Culture transplants (injection) and arrives self patient part again after 3-5 weeks, reach the purpose for substituting damaged cell reparation, by cell
Reparation build " self-healing function " of human body again, and establish improve and powerful immune system on this basis.
Cell therapy pre-treatment：
(1) reagent is added：Before separating pipe and the assembling of separating pipe lid, the cell-preservation liquid, separation gel, separation of blood will be preserved
Liquid is added in separating pipe, and is vacuum-packed, aseptic process.
(2) separating pipe assembles：Component is assembled into shown in attached drawing 1 by attached drawing 2.
(3) it samples：Patient's arm is sterilized, in the blood vessel with one insertion patient of aseptic blood taking needle, a connection cell is protected
Separating pipe is deposited, blood is flowed by suction function and mixed with the preservation liquid in separating pipe, and blood sets 30ml according to negative pressure in pipe
Sampling quantity, sample, which adopts into pipe at mark scale negative pressure and releases to sample, to be terminated.Preservation liquid in separating pipe contains anti-coagulants
Blood sample is set not solidify.
(4) it transports：Preservation liquid in pipe preserves cell integrity in transportational process, and cellular damage is avoided to influence cytoplasm
Amount.Separation gel in pipe plays the role of blood and separating liquid separates, and avoids influencing layered effect.
(5) it centrifuges：It is centrifuged by adaptable centrifuge adjustment rotating speed, time, waits cell and examination after centrifuging
Agent is layered by proportion, is calculated according to the blood plasma and cell proportion of people's blood, and karyocyte (tunica albuginea) layer detaches all aggregations
The neck of pipe, collects effective cell to the greatest extent.Entire cell pre-treatment flow does not have to tube used for bottom pouring lid, ensure that the nothing of blood
Bacterium operates.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Within the spirit and principle of utility model, any modification, equivalent replacement, improvement and so on should be included in the utility model
Protection domain within.
Claims (7)
1. a kind of cell collection preserves separating pipe, which is characterized in that coordinate including separating pipe (2) and with separating pipe (2) nozzle
Separating pipe lid (1), be equipped with integrally formed separation neck (3), and the internal diameter of separation neck (3) in the middle part of the separating pipe (2)
It is respectively smaller than the internal diameter of the separating pipe of separation neck (3) upper and lower part.
2. a kind of cell collection as described in claim 1 preserves separating pipe, which is characterized in that separation neck (3) top
Separating pipe with lower part is cylindrical shape, and the internal diameter of the cylinder on top is less than the internal diameter of the cylinder of lower part.
3. a kind of cell collection as described in claim 1 preserves separating pipe, which is characterized in that separating pipe (2) bottom is
Oval bottom is transparent material, convenient for observation centrifugal effect.
4. a kind of cell collection as described in claim 1 preserves separating pipe, which is characterized in that the separating pipe lid (1) is rubber
Glue material matter is sealed in separating pipe (2) nozzle, for keeping the negative pressure in separating pipe.
5. a kind of cell collection as described in claim 1 preserves separating pipe, which is characterized in that the separating pipe (2) is equipped with
Label labeling (5).
6. a kind of cell collection as described in claim 1 preserves separating pipe, which is characterized in that the separating pipe (2) is transparent
Material and pipe shaft are equipped with scale marker (4).
7. a kind of cell collection as described in claim 1 preserves separating pipe, which is characterized in that prepackage in the separating pipe (2)
There are the cell-preservation liquid (6), separation gel (7), separating liquid (8) of separation preservation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820341593.1U CN208087619U (en) | 2018-03-13 | 2018-03-13 | A kind of cell collection preservation separating pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820341593.1U CN208087619U (en) | 2018-03-13 | 2018-03-13 | A kind of cell collection preservation separating pipe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208087619U true CN208087619U (en) | 2018-11-13 |
Family
ID=64062404
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820341593.1U Active CN208087619U (en) | 2018-03-13 | 2018-03-13 | A kind of cell collection preservation separating pipe |
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| Country | Link |
|---|---|
| CN (1) | CN208087619U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110064230A (en) * | 2019-05-31 | 2019-07-30 | 朱德新 | A kind of platelet rich plasma acquisition separation vessel |
-
2018
- 2018-03-13 CN CN201820341593.1U patent/CN208087619U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110064230A (en) * | 2019-05-31 | 2019-07-30 | 朱德新 | A kind of platelet rich plasma acquisition separation vessel |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231102 Address after: 519000 room 1301, building 3, scientific research incubation building, phase II, Tsinghua Science Park, 101 University Road, Tangjiawan Town, high tech Zone, Zhuhai, Guangdong Patentee after: Zhuhai Langtai Biotechnology Co.,Ltd. Address before: 430000 floors 1-2, plant 2, economic development zone, Hannan District, Wuhan City, Hubei Province (Wuhan Gaoyuan Biotechnology Development Co., Ltd.) Patentee before: WUHAN DONGFANG HUAKANG TECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right |