CN108654142A - A kind of platelet rich plasma separator and its separation method - Google Patents
A kind of platelet rich plasma separator and its separation method Download PDFInfo
- Publication number
- CN108654142A CN108654142A CN201810856953.6A CN201810856953A CN108654142A CN 108654142 A CN108654142 A CN 108654142A CN 201810856953 A CN201810856953 A CN 201810856953A CN 108654142 A CN108654142 A CN 108654142A
- Authority
- CN
- China
- Prior art keywords
- platelet
- adjustable
- extraction element
- red blood
- blood cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
Abstract
The present invention relates to a kind of platelet rich plasma separator and its separation methods, including centrifuge tube, transparent identification isocon, adjustable red blood cell extraction element and adjustable rich platelet extraction element, wherein transparent identification isocon includes observation chamber, an inlet, the first liquid outlet and the second liquid outlet, and hollow needle is arranged in inlet；Adjustable red blood cell extraction element includes extracting unit, and for drawing and collecting the red blood cell layer in centrifuge tube, adjustable red blood cell extraction element is connected to the sealing of the first liquid outlet；Adjustable rich platelet extraction element includes extracting unit, and for drawing and collecting leucocyte and platelet layer in centrifuge tube, adjustable rich platelet extraction element is connected to the sealing of the second liquid outlet.The separator of the present invention is simple in structure, efficiently uses ready-made medical equipment；Platelet rich plasma separation method based on the separator is easy to operate, low to the skill requirement of operating personnel, can effectively improve platelet rich plasma quality and extraction efficiency.
Description
Technical field
The invention belongs to the field of medical instrument technology, and in particular to a kind of platelet rich plasma separator and its separation side
Method.
Background technology
Platelet rich plasma is the platelet concentrate that self whole blood obtains after centrifugation, is contained in platelet rich plasma big
Measure growth factor and protein, can accurately position oneself damage location, control inflammation, can never Tongfang in face of cell and
Facilitation is played in regeneration, to accelerate the reparation of injury tissue, is widely used in clinic, for example, Bones and joints reparation,
In the fields such as the reparation of large-area burns skin and the U.S. shaping of doctor.
In the prior art, by extracting the venous whole of patient, the highly concentrated of autologous patient can be obtained through secondary centrifuging method
Platelet rich plasma is spent, secondary centrifuging method is to obtain the generally acknowledged most effectual way of platelet rich plasma, i.e., first through red blood cell
Red blood cell is sucked out discarded or makees other purposes by red blood cell after being centrifuged to lowest level from bottommost after centrifugation, then rich platelet
Platelet rich plasma (leucocyte, blood platelet and fibrin) is centrifuged to lowest level and for use, residue is sucked out after blood plasma time centrifugation
Serum it is discarded or make other purposes.
But platelet rich plasma extraction element in the prior art has the following disadvantages：
In the prior art, operating personnel during centrifuge tube extracts red blood cell are taken out after red blood cell centrifugation to need to observe
The case where position on suction pipe head and cell are layered cannot accidentally inhale.But work as suction since red blood cell is not transparent under reality
Pipe is not easy to observe after entering, it is difficult to which clear view arrives the position of suction nozzle, be easy to cause accidentally suction, very to the skill requirement of operating personnel
It is high；Also there can be the problems such as recovery rate of blood platelet is relatively low, cannot be precisely separated.
Invention content
For the technical problems in the prior art, the purpose of the present invention is to provide a kind of separation of platelet rich plasma
Device and its separation method, the separator is simple in structure, efficiently uses ready-made medical equipment, reaches and reduces maloperation, carries
The extraction quality of platelet rich plasma.
To achieve the above object, the present invention is achieved by the following technical solutions：
First purpose of the present invention is to propose a kind of platelet rich plasma separator, including centrifuge tube, transparent distinguishes
Know isocon, adjustable red blood cell extraction element and adjustable rich platelet extraction element, wherein：
Transparent identification isocon includes observation chamber, an inlet, the first liquid outlet and the second liquid outlet, the inlet
Hollow needle for being pierced into centrifugation bottom of the tube is set；
The adjustable red blood cell extraction element includes extracting unit, for drawing and collecting the red blood cell layer in centrifuge tube,
The adjustable red blood cell extraction element is connected to the sealing of the first liquid outlet；
The adjustable rich platelet extraction element includes extracting unit, for draw and collect the leucocyte in centrifuge tube and
Platelet layer, the adjustable rich platelet extraction element are connected to the sealing of the second liquid outlet.
Further, the adjustable red blood cell extraction element is syringe.
Further, the adjustable rich platelet collection device is syringe.
Further, female Luer is arranged in first liquid outlet.
Further, it the female Luer and the first liquid outlet integrated molding or is detachably connected.
Further, female Luer is arranged in second liquid outlet.
Further, it the female Luer and the second liquid outlet integrated molding or is detachably connected.
It is another object of the present invention to propose a kind of separation method of platelet rich plasma, the separation method use
The platelet rich plasma separator of any of the above-described, includes the following steps：
(1) it uses in injector for medical purpose venous blood samples to centrifuge tube, centrifuge tube is put into centrifuge and is centrifuged；
(2) until red blood cell, leucocyte and blood platelet, serum in centrifuge in centrifuge tube take out centrifuge tube after being layered；
(3) start adjustable red blood cell extraction element and extract red blood cell layer, observed by the observation chamber of transparent identification isocon
The mobility status of red blood cell closes adjustable red blood cell extraction element after red blood cell is all pumped into adjustable red blood cell extraction element；
(4) start adjustable rich platelet extraction element and extract leucocyte and platelet layer, pass through transparent identification isocon
The mobility status for observing chamber observation leucocyte and blood platelet, when leucocyte and blood platelet are all pumped into adjustable rich platelet extraction dress
It postpones, you can it is for use to remove adjustable rich platelet extraction element.
Compared with prior art, the beneficial effects of the present invention are：
(1) platelet rich plasma separator of the invention is simple in structure, can efficiently use ready-made medical equipment, reach
The purpose for reducing maloperation, improving platelet rich plasma extraction quality.
(1) platelet rich plasma separation method of the invention is easy to operate, low to the skill requirement of operating personnel, can be effective
Improve platelet rich plasma quality and extraction efficiency.
Description of the drawings
Fig. 1 is the structural schematic diagram of the platelet rich plasma separator of the embodiment of the present invention 1.
Fig. 2 is the transparent identification isocon structural schematic diagram of the platelet rich plasma separator of the embodiment of the present invention 1.
Specific implementation mode
It shows that example illustrates certain embodiments of the present invention, and should not be construed as the model of the limitation present invention
It encloses.Present disclosure can be improved from material, method and reaction condition simultaneously, all these improvement should all
It falls within the spirit and scope of the present invention.
Embodiment 1
It is a kind of platelet rich plasma separator as shown in Figure 1, including centrifuge tube 1, transparent identification isocon 2, adjustable
Red blood cell extraction element 3 and adjustable rich platelet extraction element 4.Adjustable red blood cell extraction element 3 is for drawing and collecting centrifugation
Red blood cell layer in pipe 1, adjustable rich platelet extraction element 4 is for drawing and collecting leucocyte and blood platelet in centrifuge tube 1
Layer, adjustable red blood cell extraction element 3 and adjustable rich platelet extraction element 4 are general medical syringe.As shown in Fig. 2,
Transparent identification isocon 2 is three-port structure, including observes chamber 2-1, an inlet 2-2, the first liquid outlet 2-3 and second and go out liquid
Hollow needle 2-5 is set on mouth 2-4, inlet 2-2, for being pierced into 1 bottom of centrifuge tube.First liquid outlet 2-3 and second goes out liquid
Upper one female Luer of each installation of mouthful 2-4 is respectively sealed in each female Luer with a syringe and is connected to.The observation of isocon 2
One end of chamber 2-1 is equipped with silica gel plug 5
For transparent identification isocon 2 in the present embodiment in processing, female Luer goes out liquid with the first liquid outlet 2-3, second
Mouthful 2-4 can also be that female Luer is removably separately mounted to the first liquid outlet 2-3, second goes out liquid with integrated molding
Mouth 2-4.
It should be further noted that the adjustable red blood cell extraction element 3 in the present invention and adjustable rich platelet extraction dress
It includes but is not limited to the general medical syringe mentioned in embodiment 1 to set 4, can also be other with Liquid extracting unit
Device, such as the conduit for being connected with small-sized self priming pump can be installed at two liquid outlets, tube at one end is tightly connected out liquid
Mouthful, the other end connects a blood and receives container, and the red blood cell layer or leucocyte that extract in centrifuge tube are controlled by self-priming switch pump
And platelet layer.
Embodiment 2
The method for carrying out platelet rich plasma separation using the separator of embodiment 1, specifically includes following steps：
1, in injector for medical purpose venous blood samples to centrifuge tube 1, centrifuge tube 1 is put into centrifuge and is centrifuged.
2, until red blood cell, leucocyte and blood platelet, serum in centrifuge in centrifuge tube 1 take out centrifuge tube after being layered,
It is uppermost be serum layer, it is intermediate be leucocyte and platelet layer, nethermost red blood cell layer.
3, red blood cell syringe is connected in the female Luer of the first liquid outlet 2-3, rich platelet is collected and is noted
Emitter is connected in the female Luer of the second liquid outlet 2-4；Hollow needle 2-5 is pierced into the bottom of centrifuge tube 1.It slowly extracts out red
Cell collects the piston rod of syringe, at this moment can clearly observe red blood cell by the identification isocon 2 of transparent material
Mobility status, after red blood cell is all pumped into red blood cell syringe, just only surplus serum layer above and following in centrifuge tube 1
Leucocyte and platelet layer.
4, it slowly extracts the piston rod that rich platelet collects syringe out, again may be by the identification isocon of transparent material
2 clearly observe the mobility status of leucocyte and blood platelet, when leucocyte and blood platelet are all pumped into rich platelet and collect note
After emitter, you can it is for use to remove rich platelet collection syringe.
The platelet rich plasma separator of the present invention is simple in structure, can efficiently use ready-made medical equipment, reach and subtract
Few maloperation, the purpose for improving platelet rich plasma extraction quality.The platelet rich plasma separation method of the present invention is easy to operate,
It is low to the skill requirement of operating personnel, platelet rich plasma quality and extraction efficiency can be effectively improved.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of platelet rich plasma separator, it is characterised in that：Including centrifuge tube (1), transparent identification isocon (2), can
Red blood cell extraction element (3) and adjustable rich platelet extraction element (4) are adjusted, wherein：
Transparent identification isocon (2) goes out including observation chamber (2-1), an inlet (2-2), the first liquid outlet (2-3) and second
The hollow needle (2-5) for being pierced into centrifuge tube (1) bottom is arranged in liquid mouth (2-4), the inlet (2-2)；
The adjustable red blood cell extraction element (3) includes extracting unit, for drawing and collecting the red blood cell in centrifuge tube (1)
Layer, the adjustable red blood cell extraction element (3) are connected to the first liquid outlet (2-3) sealing；
The adjustable rich platelet extraction element (4) includes extracting unit, for drawing and collecting the leucocyte in centrifuge tube (1)
And platelet layer, the adjustable rich platelet extraction element (4) are connected to the second liquid outlet (2-4) sealing.
2. a kind of platelet rich plasma separator according to claim 1, it is characterised in that：The adjustable red blood cell carries
It is syringe to take device (3).
3. a kind of platelet rich plasma separator according to claim 1, it is characterised in that：The adjustable rich platelet
Collection device (4) is syringe.
4. a kind of platelet rich plasma separator according to claim 1, it is characterised in that：First liquid outlet
Female Luer is arranged in (2-3).
5. a kind of platelet rich plasma separator according to claim 4, it is characterised in that：The female Luer and the
One liquid outlet (2-3) integrated molding is detachably connected.
6. a kind of platelet rich plasma separator according to claim 1, it is characterised in that：Second liquid outlet
Female Luer is arranged in (2-4).
7. a kind of platelet rich plasma separator according to claim 6, it is characterised in that：The female Luer and the
Two liquid outlets (2-4) integrated molding is detachably connected.
8. a kind of separation method of platelet rich plasma, which is characterized in that the separation method is using any one of claim 1-7
Platelet rich plasma separator, include the following steps：
(1) it uses in injector for medical purpose venous blood samples to centrifuge tube (1), centrifuge tube (1) is put into centrifugation in centrifuge；
(2) until red blood cell, leucocyte and blood platelet, serum in centrifuge in centrifuge tube (1) take out centrifuge tube after being layered
(1)；
(3) start adjustable red blood cell extraction element (3) and extract red blood cell layer, pass through the observation chamber (2- of transparent identification isocon (2)
1) mobility status for observing red blood cell closes adjustable red blood cell after red blood cell is all pumped into adjustable red blood cell extraction element (3)
Extraction element (3)；
(4) start adjustable rich platelet extraction element (4) and extract leucocyte and platelet layer, pass through transparent identification isocon (2)
Observation chamber (2-1) observation leucocyte and blood platelet mobility status, when to be all pumped into adjustable rich blood small for leucocyte and blood platelet
After plate extraction element (4), you can it is for use to remove adjustable rich platelet extraction element (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810856953.6A CN108654142A (en) | 2018-07-31 | 2018-07-31 | A kind of platelet rich plasma separator and its separation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810856953.6A CN108654142A (en) | 2018-07-31 | 2018-07-31 | A kind of platelet rich plasma separator and its separation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108654142A true CN108654142A (en) | 2018-10-16 |
Family
ID=63789692
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810856953.6A Pending CN108654142A (en) | 2018-07-31 | 2018-07-31 | A kind of platelet rich plasma separator and its separation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108654142A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110699240A (en) * | 2019-11-13 | 2020-01-17 | 大连大学 | Red blood cell separator |
| CN112221724A (en) * | 2020-09-01 | 2021-01-15 | 刘勇 | Automatic blood separation system and method |
| CN114146827A (en) * | 2021-11-26 | 2022-03-08 | 南京双威生物医学科技有限公司 | One-time centrifugal preparation method of platelet-rich plasma |
| CN117126716A (en) * | 2023-09-06 | 2023-11-28 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5108549A (en) * | 1989-02-25 | 1992-04-28 | Gkss-Forschungszentrum Geesthacht Gmbh | Method of separating and recovering components of mixtures via pervaporization |
| CN1583213A (en) * | 2004-06-11 | 2005-02-23 | 西安瑞捷生物医疗技术有限公司 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
| CN101940983A (en) * | 2005-06-22 | 2011-01-12 | 科安比司特公司 | In centrifugal separator, composite fluid is separated into one group of bag of at least two kinds of compositions |
| CN102497896A (en) * | 2009-07-16 | 2012-06-13 | 巴奥米特生物公司 | Method and apparatus for separating biological materials |
| CN202860184U (en) * | 2012-09-11 | 2013-04-10 | 天津美德太平洋科技有限公司 | Separator for PRP (Platelet Rich Rlasma) in blood |
| KR101333789B1 (en) * | 2012-11-07 | 2013-11-29 | 김이선 | Separating vials and their uses |
| CN104548232A (en) * | 2013-10-15 | 2015-04-29 | 金鸿 | Device and method of extracting high-concentration plasma from whole blood |
| CN204601700U (en) * | 2015-04-08 | 2015-09-02 | 武汉维斯第医用科技股份有限公司 | A kind of extraction and isolation equipment for platelet rich plasma and disposable collecting system |
| CN104971828A (en) * | 2015-05-07 | 2015-10-14 | 上海交通大学附属第六人民医院 | Centrifuge device for separating blood components and platelet-rich plasma manufacturing method |
| CN107096651A (en) * | 2017-07-01 | 2017-08-29 | 张方亮 | Prepare the separator and method of platelet rich plasma |
| CN208839092U (en) * | 2018-07-31 | 2019-05-10 | 宁波爱德康生物科技有限公司 | A kind of platelet rich plasma separator |
-
2018
- 2018-07-31 CN CN201810856953.6A patent/CN108654142A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5108549A (en) * | 1989-02-25 | 1992-04-28 | Gkss-Forschungszentrum Geesthacht Gmbh | Method of separating and recovering components of mixtures via pervaporization |
| CN1583213A (en) * | 2004-06-11 | 2005-02-23 | 西安瑞捷生物医疗技术有限公司 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
| CN101940983A (en) * | 2005-06-22 | 2011-01-12 | 科安比司特公司 | In centrifugal separator, composite fluid is separated into one group of bag of at least two kinds of compositions |
| CN102497896A (en) * | 2009-07-16 | 2012-06-13 | 巴奥米特生物公司 | Method and apparatus for separating biological materials |
| CN202860184U (en) * | 2012-09-11 | 2013-04-10 | 天津美德太平洋科技有限公司 | Separator for PRP (Platelet Rich Rlasma) in blood |
| KR101333789B1 (en) * | 2012-11-07 | 2013-11-29 | 김이선 | Separating vials and their uses |
| CN104548232A (en) * | 2013-10-15 | 2015-04-29 | 金鸿 | Device and method of extracting high-concentration plasma from whole blood |
| CN204601700U (en) * | 2015-04-08 | 2015-09-02 | 武汉维斯第医用科技股份有限公司 | A kind of extraction and isolation equipment for platelet rich plasma and disposable collecting system |
| CN104971828A (en) * | 2015-05-07 | 2015-10-14 | 上海交通大学附属第六人民医院 | Centrifuge device for separating blood components and platelet-rich plasma manufacturing method |
| CN107096651A (en) * | 2017-07-01 | 2017-08-29 | 张方亮 | Prepare the separator and method of platelet rich plasma |
| CN208839092U (en) * | 2018-07-31 | 2019-05-10 | 宁波爱德康生物科技有限公司 | A kind of platelet rich plasma separator |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110699240A (en) * | 2019-11-13 | 2020-01-17 | 大连大学 | Red blood cell separator |
| CN112221724A (en) * | 2020-09-01 | 2021-01-15 | 刘勇 | Automatic blood separation system and method |
| CN112221724B (en) * | 2020-09-01 | 2022-07-08 | 刘勇 | Automatic blood separation system and method |
| CN114146827A (en) * | 2021-11-26 | 2022-03-08 | 南京双威生物医学科技有限公司 | One-time centrifugal preparation method of platelet-rich plasma |
| CN114146827B (en) * | 2021-11-26 | 2024-01-23 | 南京双威生物医学科技有限公司 | One-time centrifugation preparation method of platelet-rich plasma |
| CN117126716A (en) * | 2023-09-06 | 2023-11-28 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
| CN117126716B (en) * | 2023-09-06 | 2024-04-05 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108654142A (en) | A kind of platelet rich plasma separator and its separation method | |
| CN206523325U (en) | A kind of saliva collection save set | |
| CN202478263U (en) | Device for extracting, separating and injecting serum from blood | |
| CN208839092U (en) | A kind of platelet rich plasma separator | |
| CN105596014A (en) | Double-cannula centrifugal injector | |
| CN102242054B (en) | Totally-enclosed cell and cell factor preparation device and preparation method thereof | |
| WO2015108273A1 (en) | Apparatus and method for separating blood for extraction of platelet rich plasma | |
| CN209645525U (en) | Controllable multichannel flushing aspirator with separator | |
| CN205494504U (en) | Autologous red blood cell collection system | |
| US20170224318A1 (en) | Dual Syringe with Percutaneous Cannula | |
| US10245357B2 (en) | System and kit for the collection and processing of amniotic fluid and placental aspirate | |
| US20180199923A9 (en) | Percutaneous Aspirating Cannula | |
| CN209422595U (en) | Detachable piston type acquisition separation injecting medicinal platelet rich plasma preparation facilities | |
| CN210056041U (en) | AIDS blood extraction device for preventing cross infection | |
| CN210843343U (en) | Tumor sampling device convenient for tumor separation | |
| CN208838027U (en) | A kind of suction lipectomy device can provide continuous negative pressure | |
| CN209713786U (en) | The multichannel main body of controllable multichannel flushing aspirator with separator | |
| JP2017530789A (en) | Flowing blood cleaning system and method | |
| CA2543702A1 (en) | A placental-blood extraction device | |
| CN211752682U (en) | Aseptic blood cell separator | |
| CN204500724U (en) | A kind of can the remaining needle of Real-time Collection blood or administration | |
| CN208823579U (en) | A kind of platelet rich plasma separator | |
| CN109646731A (en) | The suction skirt component of controllable multichannel flushing aspirator with separator | |
| CN105343946A (en) | Cyst fluid suction device | |
| CN209123034U (en) | It is a kind of based on single milling machine control horses adopt sizing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |