CN203639459U - Tissue processor for separating living cells - Google Patents
Tissue processor for separating living cells Download PDFInfo
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- CN203639459U CN203639459U CN201320564671.1U CN201320564671U CN203639459U CN 203639459 U CN203639459 U CN 203639459U CN 201320564671 U CN201320564671 U CN 201320564671U CN 203639459 U CN203639459 U CN 203639459U
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- tissue processor
- homogenate
- blade
- tissue
- rotating head
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Abstract
The utility model relates to a tissue processor for separating living cells from animal and plant tissues. The tissue processor comprises a tissue processor control device and a tissue processor homogenate vessel, wherein the tissue processor control device comprises a machine body (1), a homogenate vessel placing hole (2), a rotary head (3), a clamping slot (4) and a heat dissipating hole (5), the tissue processor homogenate vessel comprises a cup body (6), a cup cover (7), a sealing ring (8), a rotary head connector (9), a fixed shaft (10), cutter head (11) and a chuck (12). The tissue processor is convenient to operate, can process 10-600ml volume of samples and 5-400g of samples once, improves the processing efficiency of large tissues and the obtaining rate of living cells, can easily protect the separated samples from bacteria, and is an ideal tissue processor separating living cells from large tissues.
Description
Technical field
The utility model relates to a kind of cell separation apparatus of animal vegetable tissue, more specifically says a kind of tissue processor that separates viable cell from animal vegetable tissue.
Background technology
Along with the develop rapidly of life science, cell is being brought into play more and more important effect at aspects such as basic scientific research, biological medicine and clinical applications.The cell of vitro culture all separates and obtains from bio-tissue, and the traditional method that is at present individual cells by separate tissue has mechanical process, enzyme digestion and both to combine etc., the standard that these methods are ununified, and lose time very much.
In order to realize automatization, stdn and simple and directization from separate tissue cell manipulation, some companies start to develop full automatic cell separator.There is in the market the separate tissue of a small amount of full automatic cell separator for single suspension viable cell.The full-automatic tissue processor of gentleMACS of Mei Tian Ni company of Germany, can be gentle, safely, save time, the isolated mononuclear cell suspension from tissue of automatization.This equipment uses special disposable separator tube.Pipe covers with the rotor of particular design and stator, is organized into unicellular for homogenate.Tube hub has the aperture of diaphragm seals, can ensure under aseptic condition processing sample safely in closed system.Do not need to open test tube cap completely, can add enzyme and other solution, or take out the cell or the homogenate solution that separate.Can process the sample of 0.3-10ml capacity, the sample of 20-4000mg size.GentleMACS is for separating except the unicellular of tissue used of bone and cartilaginous tissue, as tissues such as brain, liver, kidney, blood vessel endothelium, skin, muscle, heart, solid tumors at present.The cell separator of WISBiomed company of the U.S., has safety, anti-pollution, can carry out single sample processing.Special closed test tube, has the cutter head of separation by separate tissue, obtains cell.Sample that can primary treatment 2-15ml liquid capacity.
These full-automatic cell separators are mainly used in the fundamental research of cell, therefore organize and mostly derive from the animal body that volume is little, as mouse, rat etc.; In addition, need tissue volume to be processed and amount also smaller, maximum is also with regard to tens grams.
Utility model content
The technical problems to be solved in the utility model is how on existing automatic tissue homogenate instrument principle of work basis, and by cutter head and the isostructural appropriate design of refiner capacity, acquisition single cell suspension is organized in disposable processing in a large number.The tissue processor that the utility model is developed once can be processed the sample of 10-600ml capacity, the sample of 5-400g size.
In order to realize above goal of the invention, living cell tissue of the present utility model treater comprises control device and homogenate container, as shown in Figure 1.
Control device is for controlling the operation of tissue treatment apparatus, and its structure mainly comprises fuselage (1), homogenate container putting hole (2), rotating head (3), draw-in groove (4), louvre (5).
Fuselage (1) is for the protection of the circuit part of tissue treatment apparatus;
Homogenate container putting hole (2) is positioned at fuselage (1) top, and its internal diameter is slightly larger than tissue processor homogenate its external diameter maximum, for placing homogenate container;
Rotating head (3) is positioned at homogenate container putting hole (2) inside bottom, be connected with circuit part, the clamp of projection that its upper end has several (being no less than 1), for being connected with tissue processor homogenate container, drive the rotation of cutter head in homogenate container (11) by circuit;
Draw-in groove (4) is positioned at homogenate container putting hole (2) inwall, for fixing organization treater homogenate container, makes tissue processor when running homogenate integral container can the transfer with the rotation of rotating head;
Louvre (5) is in the side of fuselage (1), for the heat radiation of instrument.
Homogenate container is for the loading of tissue samples, and under the control of control device, by the rotation of the built-in cutter head of container, make tissue samples homogenate, and directly obtain free cell suspension by filtering, its structure mainly comprises cup (6), bowl cover (7), sealing-ring (8), rotating head junctor (9), stationary shaft (10), cutter head (11) and dop (12).
Cup (6) is transparent material, and for depositing tissue samples, transparent material ensures in treating processes the state of tissues observed sample at any time;
Bowl cover (7) is connected with cup (6) by forms such as screw thread, bayonet socket, ground, running-ons, and there is aperture its central position for placing stationary shaft (10);
Sealing-ring (8) is in bowl cover (7), for play the effect of enhanced leaktightness in the time that cup (6) engages with bowl cover (7), ensure that the sample in tissue processor homogenate container can be not excessive, also ensure that the small materials such as sample process peripheral dust, microorganism can not enter in tissue processor homogenate container;
Rotating head junctor (9) vertical view as shown in Figure 5.
Rotating head junctor is positioned at the downside of bowl cover, for connecting rotating head (3) and cutter head (11).Its underpart has and the supporting quantity of rotating head (3) clamp of (being no less than 1), and for being closely connected with rotating head (3), top is connected with cutter head (11) by stationary shaft (10).
Described stationary shaft (10) is through bowl cover (7) center, connect rotating head junctor (9) and cutter head (11), and the blade (13) of fixing composition cutter head (11) and blade (14);
Described cutter head (11) side-view as shown in Figure 2.
Cutter head (11) is made up of blade (13) and blade (14), and wherein as shown in Figure 3, the side-view of blade (14) and vertical view are as shown in Figure 4 for the side-view of blade (13) and vertical view.
Blade (13) and blade (14) stacked on top of one another are placed, and orthogonal, are four leaf blades, fix in the central position of two groups of blades by stationary shaft (10), and the upper-lower position of two groups of blades is interchangeable.
Four each leaf of leaf blade have a side tool cutting edge of a knife or a sword at least, and knife edge decision design is in the working direction of conrotatory rotary head rotation.
Blade (13) divides 5 sections, comprises 4 bendings, and the bending of two, two ends is 120-160 degree (preferably 130-150 degree, preferably 138 degree), and middle two bendings are 130-170 degree (preferably 140-160 spend, preferably 148 degree);
Blade (14) divides 3 sections, comprises 2 bendings, and two bendings are 100-140 degree (preferably 110-130 degree, preferably 123 degree);
Bayonet socket (12) is positioned at cup (6) above and the junction of bowl cover (7), corresponding supporting with draw-in groove (4), for fixing homogenate container.
A kind of tissue processor that separates viable cell, this device comprises control device and homogenate container, control device is by fuselage (1), homogenate container putting hole (2), rotating head (3), draw-in groove (4), louvre (5) composition, homogenate container is by cup (6), bowl cover (7), sealing-ring (8), rotating head junctor (9), stationary shaft (10), cutter head (11) and dop (12) composition, it is characterized in that fuselage (1) side is provided with louvre (5), fuselage (1) top is homogenate container putting hole (2), homogenate container putting hole (2) inwall is provided with draw-in groove (4), homogenate container putting hole (2) bottom is rotating head (3), when processing is organized, homogenate container is placed in homogenate container putting hole (2), rotating head junctor (9) lower end of bowl cover (7) bottom engages with rotating head (3), upper end is connected with cutter head (11) by stationary shaft (10), bowl cover (7) is by sealing-ring (8) and cup body (6) tight joint, the dop (12) of cup body (6) outer side wall engages with draw-in groove (4).
Described tissue processor, is characterized in that rotating head (3) engages with rotating head junctor (9) by protruding clamp, and the clamp number of projection is 4.
Described tissue processor, it is characterized in that described cutter head (11) is by two blades: the first blade (13) forms with the second blade (14), two groups of blade stacked on top of one another are placed, orthogonal, be four leaf blade forms, the upper-lower position of two groups of blades is interchangeable.
Described tissue processor, is characterized in that the first blade (13) divides 5 sections, comprises 4 bendings, and the bending of two, two ends is 120-160 degree, and middle two bendings are 130-170 degree; The second blade (14) divides 3 sections, comprises 2 bendings, and two bendings are 100-140 degree.
Described tissue processor, is characterized in that this device once can process the sample of 10-600ml capacity, the sample of 5-400g size.
The beneficial effects of the utility model: the tissue processor of the utility model development once can be processed the sample of 10-600ml capacity, the sample of 5-400g size, has improved the processing efficiency of bulk tissue; The improvement of blade shapes, has improved the pick-up rate of viable cell in tissue; Absolute construction and the composition of tissue processor homogenate container, be convenient to ensure sample disinfecting in sterile state and consumptive material in treating processes.
Brief description of the drawings
Fig. 1 tissue treatment apparatus side-view, fuselage (1), homogenate container putting hole (2), rotating head (3), draw-in groove (4), louvre (5), cup (6), bowl cover (7), sealing-ring (8), rotating head junctor (9), stationary shaft (10), cutter head (11), dop (12).
Fig. 2 tissue processor homogenate container cutter head (11) side-view
Fig. 3 blade (13) side-view and vertical view
Fig. 4 blade (14) side-view and vertical view
Fig. 5 rotating head junctor (9) vertical view
Embodiment
Tissue processor of the present utility model comprises tissue processor control device and tissue processor homogenate container, as shown in Figure 1, tissue processor control device comprises fuselage (1), homogenate container putting hole (2), rotating head (3), draw-in groove (4), louvre (5), tissue processor homogenate container comprises cup (6), bowl cover (7), sealing-ring (8), rotating head junctor (9), stationary shaft (10), cutter head (11), dop (12).
When operation, get tissue samples, after cleaning, be cut into small pieces, put into cup (6), with 2: 1-1: 2 ratio (preferably 1: 1, mass ratio) add a certain amount of damping fluid or enzymolysis solution, cover after bowl cover (7), tissue processor homogenate container is put into homogenate container putting hole (2), switch on power, set certain rotating speed and runtime according to different tissues, tissue is broken into individual cells or cell mass, by the net filtration after filtration of organizing of pulverizing, obtains individual cells suspension or small cell cluster.
Claims (5)
1. one kind separates the tissue processor of viable cell, this device comprises control device and homogenate container, control device is by fuselage (1), homogenate container putting hole (2), rotating head (3), draw-in groove (4), louvre (5) composition, homogenate container is by cup (6), bowl cover (7), sealing-ring (8), rotating head junctor (9), stationary shaft (10), cutter head (11) and dop (12) composition, it is characterized in that fuselage (1) side is provided with louvre (5), fuselage (1) top is homogenate container putting hole (2), homogenate container putting hole (2) inwall is provided with draw-in groove (4), homogenate container putting hole (2) bottom is rotating head (3), when processing is organized, homogenate container is placed in homogenate container putting hole (2), rotating head junctor (9) lower end of bowl cover (7) bottom engages with rotating head (3), upper end is connected with cutter head (11) by stationary shaft (10), bowl cover (7) is by sealing-ring (8) and cup body (6) tight joint, the dop (12) of cup body (6) outer side wall engages with draw-in groove (4).
2. tissue processor according to claim 1, is characterized in that rotating head (3) engages with rotating head junctor (9) by protruding clamp, and the clamp number of projection is 4.
3. tissue processor according to claim 1, it is characterized in that described cutter head (11) is by two blades: the first blade (13) forms with the second blade (14), two groups of blade stacked on top of one another are placed, orthogonal, be four leaf blade forms, the upper-lower position of two groups of blades is interchangeable.
4. tissue processor according to claim 3, it is characterized in that the first blade (13) divides 5 sections, comprise 4 bendings, the bending of two, two ends is 120-160 degree, middle two bendings are 130-170 degree, the second blade (14) divides 3 sections, comprises 2 bendings, and two bendings are 100-140 degree.
5. tissue processor according to claim 1, is characterized in that this device once can process the sample of 10-600ml capacity, the sample of 5-400g size.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320564671.1U CN203639459U (en) | 2013-09-12 | 2013-09-12 | Tissue processor for separating living cells |
| TW103216276U TWM499555U (en) | 2013-09-12 | 2014-09-12 | Tissue treatment device for separating living cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320564671.1U CN203639459U (en) | 2013-09-12 | 2013-09-12 | Tissue processor for separating living cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203639459U true CN203639459U (en) | 2014-06-11 |
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ID=50870721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320564671.1U Expired - Lifetime CN203639459U (en) | 2013-09-12 | 2013-09-12 | Tissue processor for separating living cells |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN203639459U (en) |
| TW (1) | TWM499555U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296324A (en) * | 2014-07-08 | 2016-02-03 | 吉林化工学院 | Efficient mitochondrion separator |
| WO2016095390A1 (en) * | 2014-12-16 | 2016-06-23 | 南通市肿瘤医院 | Automatic histocyte separator |
| CN109790504A (en) * | 2016-09-29 | 2019-05-21 | 高雄医学大学 | One kind dividing cellifugal device from tissue |
| CN111548919A (en) * | 2020-05-21 | 2020-08-18 | 湖南省天骑医学新技术股份有限公司 | Magnetic control type tissue crushing bacteria extraction device and extraction method |
| CN112391346A (en) * | 2020-11-30 | 2021-02-23 | 中国医学科学院医学生物学研究所 | Isolated culture method for obtaining tree shrew brain astrocyte by non-enzymatic digestion method |
-
2013
- 2013-09-12 CN CN201320564671.1U patent/CN203639459U/en not_active Expired - Lifetime
-
2014
- 2014-09-12 TW TW103216276U patent/TWM499555U/en unknown
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296324A (en) * | 2014-07-08 | 2016-02-03 | 吉林化工学院 | Efficient mitochondrion separator |
| WO2016095390A1 (en) * | 2014-12-16 | 2016-06-23 | 南通市肿瘤医院 | Automatic histocyte separator |
| CN109790504A (en) * | 2016-09-29 | 2019-05-21 | 高雄医学大学 | One kind dividing cellifugal device from tissue |
| CN111548919A (en) * | 2020-05-21 | 2020-08-18 | 湖南省天骑医学新技术股份有限公司 | Magnetic control type tissue crushing bacteria extraction device and extraction method |
| CN111548919B (en) * | 2020-05-21 | 2024-04-16 | 湖南省天骑医学新技术股份有限公司 | Magnetic control type tissue crushing bacteria extraction device and extraction method |
| CN112391346A (en) * | 2020-11-30 | 2021-02-23 | 中国医学科学院医学生物学研究所 | Isolated culture method for obtaining tree shrew brain astrocyte by non-enzymatic digestion method |
| CN112391346B (en) * | 2020-11-30 | 2024-03-29 | 中国医学科学院医学生物学研究所 | Separation culture method for obtaining tree shrew brain astrocyte by non-enzymatic digestion method |
Also Published As
| Publication number | Publication date |
|---|---|
| TWM499555U (en) | 2015-04-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140611 |
|
| CX01 | Expiry of patent term |