CN111548919B - Magnetic control type tissue crushing bacteria extraction device and extraction method - Google Patents
Magnetic control type tissue crushing bacteria extraction device and extraction method Download PDFInfo
- Publication number
- CN111548919B CN111548919B CN202010434968.0A CN202010434968A CN111548919B CN 111548919 B CN111548919 B CN 111548919B CN 202010434968 A CN202010434968 A CN 202010434968A CN 111548919 B CN111548919 B CN 111548919B
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- container
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- rotating seat
- magnetic
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- 238000000605 extraction Methods 0.000 title claims abstract description 23
- 241000894006 Bacteria Species 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims description 14
- 239000006166 lysate Substances 0.000 claims description 7
- 239000012634 fragment Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 4
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 3
- 244000052616 bacterial pathogen Species 0.000 claims 2
- 238000010298 pulverizing process Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 8
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 3
- 238000012864 cross contamination Methods 0.000 abstract description 2
- 238000002558 medical inspection Methods 0.000 abstract description 2
- 241000304886 Bacilli Species 0.000 description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000005336 cracking Methods 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 206010011409 Cross infection Diseases 0.000 description 3
- 206010029803 Nosocomial infection Diseases 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a magnetic control type tissue crushing bacteria extraction device and an extraction method, which belong to the field of medical inspection equipment, and comprise a container, wherein a magnetic rotation driving device is arranged at the bottom of the container; an impeller driven to rotate by a magnetic rotation driving device is arranged in the container; the magnetic force rotation driving device is formed by connecting a rotating motor with a rotating seat; the rotating seat is provided with a magnet for sucking the impeller. The method has the main advantages of remarkably improving the discovery rate of the acid-fast bacillus, along with low product cost, convenient use and capability of preventing cross contamination of detection test results.
Description
Technical Field
The invention belongs to the field of medical inspection equipment, and particularly relates to a magnetic control type tissue crushing bacteria extraction device and an extraction method.
Background
The diagnosis of pathological tissue sections is a very important and frequently used diagnosis method in the medical field, and is used in a medical environment, so that in order to avoid cross infection, all current pathological detection products must be disposable products, and a mode of detecting after tissue sections is generally adopted. However, the pathological diagnosis of acid-fast bacilli in the tissue of the section is difficult because bacteria are inlaid in the tissue, and even after the section is ultrathin, the bacteria are distributed very dispersedly and are difficult to find, so that inaccurate detection results of acid-fast bacilli in the tissue are caused, and the error rate is often required to be eliminated by improving the detection quantity.
Disclosure of Invention
Aiming at the problems, the invention provides a magnetic control type tissue-crushed bacteria extraction device and an extraction method, which have the main advantages of remarkably improving the discovery rate of acid-fast bacilli, along with low product cost, convenient use and capability of preventing cross contamination of detection test results.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the magnetic control type tissue crushing bacteria extraction device comprises a container, wherein a magnetic force rotation driving device is arranged at the bottom of the container; an impeller driven to rotate by a magnetic rotation driving device is arranged in the container; the magnetic force rotation driving device is formed by connecting a rotating motor with a rotating seat; the rotating seat is provided with a magnet for sucking the impeller.
As a further development of the invention, the rotation axis of the swivel base is perpendicular to the horizontal plane; the upper end face of the rotating seat is parallel to the bottom face of the container.
As a further improvement of the invention, the rotating seat is provided with two magnets which are respectively positioned at two ends of the top surface of the rotating seat.
As a further improvement of the invention, the magnetic force rotation driving device is arranged below the table top of the frame; the container is placed on a table top.
As a further improvement of the invention, the impeller is a ferrous member; the bottom of the center of the impeller is provided with a rotating fulcrum protruding downwards.
As a further improvement of the invention, the impeller is provided with at least one pair of main blades and at least one pair of auxiliary blades; the primary blade length is greater than the secondary blade.
As a further improvement of the invention, the two sides of the upper end surface of the main blade are provided with upper inclined planes; the bottom surface of the auxiliary blade is provided with a lower inclined surface.
As a further improvement of the invention, a filter screen is arranged at the outlet of the container.
As a further improvement of the invention, the mesh size of the filter screen is 100-120 meshes.
A magnetic control type tissue crushing bacteria extraction method comprises the following operation steps:
step one, placing a tissue block to be detected and a tissue lysate into the container;
step two, placing the container on a table top and locating the container right above the rotating seat;
starting a rotating motor, and driving the impeller to rotate at a high speed by the rotation of the rotating seat through magnetic attraction, so that the impeller pulverizes tissues into paste;
and step four, pouring out the liquid containing the acid-fast bacilli in the container, and filtering out tissue fragments to obtain a liquid specimen containing the acid-fast bacilli.
The invention has the beneficial effects that:
1. the invention adopts magnetic force to separate and control the impeller to rotate at high speed, so that the device can isolate bacteria and viruses, prevent cross infection, simultaneously, the rotation of the impeller can cut tissues, the tissues can be crushed into paste, and acid-fast bacilli in the tissues are promoted to enter the lysis solution, thereby improving the accuracy of detection results of the acid-fast bacilli.
2. The impeller is freely placed in the container, the resistance is small, the auxiliary blades are arranged, the length of each auxiliary blade is shorter than that of each main blade, the balance effect can be achieved, the impeller is more stable in the rotating process, and meanwhile the rotating speed is higher.
3. According to the invention, the upper inclined surfaces are arranged on two sides of the upper end surface of the main blade, the lower inclined surfaces are arranged on the lower end surface of the auxiliary blade, and the upper inclined surfaces and the lower inclined surfaces are mutually matched, so that the friction force of the impeller is further reduced in the high-speed rotation process, the floating rotation is formed, the rotation speed of the impeller is greatly improved, and the cutting effect is improved.
4. The device can realize the function of cutting tissues, and simultaneously can realize the rotation and oscillation effects because the blades can reach very high rotating speed, thereby further promoting the discharge of bacteria in the tissues and improving the extraction effect.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic top view of a rotary base according to the present invention;
FIG. 3 is a side view of an impeller;
FIG. 4 is a top view of the impeller;
FIG. 5 is a block diagram of an impeller in another embodiment;
in the figure: 1. a container; 2. a cover; 3. a liquid outlet; 4. a filter screen; 5. an impeller; 6. a rotating seat; 7. a magnet; 8. a table top; 9. a rotating electric machine; 51. a rotation fulcrum; 52. a main blade; 53. auxiliary blades; 521. an upper inclined plane; 531. and a lower inclined plane.
Detailed Description
In order that those skilled in the art may better understand the technical solutions of the present invention, the following detailed description of the present invention with reference to the accompanying drawings is provided for exemplary and explanatory purposes only and should not be construed as limiting the scope of the present invention.
As shown in fig. 1-5, one embodiment of the present invention is structured: the magnetic control type tissue crushing bacteria extraction device comprises a container 1, wherein a magnetic force rotation driving device is arranged at the bottom of the container 1; an impeller 5 driven to rotate by a magnetic rotation driving device is arranged in the container 1; the magnetic force rotation driving device is formed by connecting a rotating motor 9 with a rotating seat 6; the rotating seat 6 is provided with a magnet 7 for attracting the impeller 5. The magnetic rotary driving device can be fixedly arranged below the bottom surface of the container 1; or may be separated from the container 1 into separate components; the container 1 can be arranged into a cup body structure, generally adopts a cylindrical shape, and the upper port is provided with a sealing cover, when the device operates, the container 1 should be in a sealing state, after the operation is finished, the outlet is opened, and liquid is filtered and collected for detection. In the invention, the impeller and the magnetic rotary driving device are connected at intervals, so that the container is more convenient to clean after the operation is finished, and the risk of cross infection can be reduced.
In a preferred embodiment, as shown in fig. 1, the rotation axis of the rotating seat 6 is perpendicular to the horizontal plane; the upper end face of the rotating seat 6 is parallel to the bottom face of the container 1. The rotating seat 6 is positioned below the bottom surface of the container 1, the magnet on the rotating seat 6 attracts the impeller, and when the rotating seat 6 rotates, the impeller synchronously rotates.
In a preferred embodiment, as shown in fig. 2, two magnets 7 are disposed on the rotating base 6 and are respectively located at two ends of the top surface of the rotating base 6, and each magnet attracts two ends of the impeller.
In a preferred embodiment, as shown in fig. 1, the magnetic rotary driving device is arranged below the table top 8 of the frame; the container 1 is placed on a table top 8.
In a preferred embodiment, in order to enable the impeller 5 to be attracted by a magnet, the impeller 5 is a ferrous member; the center bottom of the impeller 5 is provided with a rotation fulcrum 51 protruding downwards. The impeller 5 is freely placed on the bottom of the container and is supported for rotation by the rotation fulcrum 51, and the rotation is not fixed for rotation, so that the resistance to rotation is very small, and in order to improve the rotation balance of the impeller, the rotation fulcrum 51 is preferably a hemispherical shape.
In a preferred embodiment, as shown in fig. 4, at least one pair of main blades 52 and at least one pair of auxiliary blades 53 are disposed on the impeller 5; the primary vanes 52 are longer than the secondary vanes 53. The primary blades 52 are mainly used for achieving rotary oscillation and rotary cutting, and the secondary blades are used for balancing rotation of the impeller, so that the impeller can rotate more stably by means of the solution.
In a preferred embodiment, as shown in fig. 5, upper inclined surfaces 521 are disposed on both sides of the upper end surface of the main blade 52; the bottom surface of the auxiliary vane 53 is provided with a lower inclined surface 531. The two upper inclined planes 521 on the main blade 52, wherein the upper inclined plane on one side can promote the cracking liquid to rotate and simultaneously generate up-and-down flow, so that tissues in the cracking liquid can be cut by the main blade, the upper inclined plane on the other side can enable the cracking liquid to flow downwards along the upper inclined plane when the impeller rotates at a high speed, so that the cracking liquid enters the bottom of the auxiliary blade, meanwhile, the lower inclined plane 531 at the bottom of the auxiliary blade generates lifting force, and the lifting force generated by the auxiliary blade and the descending effect of the main impeller are mutually offset, so that the high-speed rotation of the impeller is less in resistance, the rotation speed of the impeller is improved, and the cutting effect of the impeller is also improved.
In a preferred embodiment, a filter screen 4 is arranged at the outlet of the container 1 for filtering out tissue in the lysate.
In a preferred embodiment, the mesh size of the filter 4 is 100-120 mesh to block most tissue fragments in order to ensure the passage of acid fast bacilli.
A magnetic control type tissue crushing bacteria extraction method comprises the following operation steps:
step one, placing a tissue block to be detected and a tissue lysate into the container 1;
step two, placing the container 1 on a table top 8 and locating the container right above the rotating seat 6;
step three, starting a rotating motor 9, and driving an impeller 5 to rotate at a high speed by magnetic attraction force through rotation of a rotating seat 6, wherein the impeller 5 pulverizes tissues into paste;
and fourthly, pouring out the liquid containing the acid-fast bacilli in the container 1, and filtering out tissue fragments to obtain a liquid specimen containing the acid-fast bacilli.
The core of the invention is: a rotatable iron blade, a tissue block to be inspected and tissue lysate are placed in a closed bacteria container. The tissue is crushed into paste in the rotating process of the blade by driving the blade to rotate at a high speed through the strong magnetic device, the paste tissue can be quickly fluffed by tissue lysate, and acid-fast bacillus is easily separated from the tissue into a solution along with the stirring effect generated by the rotation of the blade. The container cover is provided with a cup mouth with a filter screen, and tissue fragments are blocked on the filter screen when the liquid containing the acid-fast bacilli is poured out, so that a liquid sample containing the acid-fast bacilli is obtained, and further detection work is carried out.
1. The blade is arranged on a strip-shaped iron member, the iron member and the blade can be driven to rotate by a strong magnet, and two sides of the center part of the iron member are protruded to form a rotating fulcrum with the cup bottom. The strong magnet is mounted on a motor which can adjust the speed.
2. Tissue lysates come in a variety of formulations, 20% sodium hypochlorite solution or trypsin, and the like.
3. The mesh size of the cup opening filter screen is 100-120 meshes, so that the acid-fast bacillus can pass through the filter screen and most tissue fragments can be blocked.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to facilitate an understanding of the method of the present invention and its core ideas. The foregoing is merely illustrative of the preferred embodiments of this invention, and it is noted that there is objectively no limit to the specific structure disclosed herein, since numerous modifications, adaptations and variations can be made by those skilled in the art without departing from the principles of the invention, and the above-described features can be combined in any suitable manner; such modifications, variations and combinations, or the direct application of the inventive concepts and aspects to other applications without modification, are contemplated as falling within the scope of the present invention.
Claims (7)
1. The magnetic control type tissue crushing bacteria extraction device comprises a container (1), and is characterized in that a magnetic force rotation driving device is arranged at the bottom of the container (1); an impeller (5) driven to rotate by a magnetic rotation driving device is arranged in the container (1); the magnetic force rotation driving device is formed by connecting a rotating motor (9) with a rotating seat (6); a magnet (7) for sucking the impeller (5) is arranged on the rotating seat (6);
at least one pair of main blades (52) and at least one pair of auxiliary blades (53) are arranged on the impeller (5); the length of the main blade (52) is longer than that of the auxiliary blade (53);
upper inclined planes (521) are arranged on two sides of the upper end face of the main blade (52); the bottom surface of the auxiliary blade (53) is provided with a lower inclined surface (531);
the outlet of the container (1) is provided with a filter screen (4).
2. A magnetically controlled tissue morcellating bacteria extraction device according to claim 1, wherein the rotation axis of the rotatable seat (6) is perpendicular to the horizontal plane; the upper end face of the rotating seat (6) is parallel to the bottom face of the container (1).
3. The magnetic control type tissue-crushing bacteria extraction device according to claim 2, wherein the rotating seat (6) is provided with two magnets (7) which are respectively positioned at two ends of the top surface of the rotating seat (6).
4. A magnetically controlled tissue morcellating bacteria extraction device according to claim 1, wherein the magnetic rotary drive means is arranged below the table top (8) of the frame; the container (1) is placed on a table top (8).
5. A magnetically controlled tissue morcellating bacteria extraction device according to claim 1, wherein the impeller (5) is a ferrous member; the center bottom of the impeller (5) is provided with a rotating fulcrum (51) protruding downwards.
6. A magnetically controlled tissue morcellating bacteria extraction device according to claim 1, wherein the mesh size of the filter screen (4) is 100-120 mesh.
7. The extraction method of a magnetic control type tissue-pulverizing bacteria extraction device according to any one of claims 1 to 6, comprising the following steps:
step one, placing a tissue block to be detected and a tissue lysate into the container (1);
step two, placing the container (1) on a table top (8) and locating the container right above the rotating seat (6);
step three, starting a rotating motor (9), driving an impeller (5) to rotate at a high speed by the rotation of a rotating seat (6) through magnetic attraction, and crushing tissues into paste by the impeller (5);
pouring out the liquid containing the acid-fast bacilli in the container (1), and filtering out tissue fragments to obtain a liquid specimen containing the acid-fast bacilli.
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CN203639459U (en) * | 2013-09-12 | 2014-06-11 | 博雅干细胞科技有限公司 | Tissue processor for separating living cells |
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CN210449005U (en) * | 2019-04-15 | 2020-05-05 | 深圳市美雅洁技术股份有限公司 | Magnetic stirring device for tissue dehydrator |
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US10125388B2 (en) * | 2007-10-31 | 2018-11-13 | Akonni Biosystems, Inc. | Integrated sample processing system |
EP3405288A4 (en) * | 2016-01-19 | 2020-01-01 | Shanxi Zdgsy Bio-Scientific Co., Ltd. | Multifunctional biological substance separation device |
WO2018070222A1 (en) * | 2016-10-13 | 2018-04-19 | パナソニックIpマネジメント株式会社 | Biomolecule extraction method, extraction system, and extraction container |
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Patent Citations (4)
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CN203639459U (en) * | 2013-09-12 | 2014-06-11 | 博雅干细胞科技有限公司 | Tissue processor for separating living cells |
CN203632470U (en) * | 2014-01-04 | 2014-06-04 | 宁波润泽电器有限公司 | Motor rotor |
CN205676465U (en) * | 2016-06-07 | 2016-11-09 | 刘宏峰 | The anti-pollution blake bottle of clinical laboratory |
CN210449005U (en) * | 2019-04-15 | 2020-05-05 | 深圳市美雅洁技术股份有限公司 | Magnetic stirring device for tissue dehydrator |
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