CN114279775A - Sampling device for monitoring bioaerosol - Google Patents
Sampling device for monitoring bioaerosol Download PDFInfo
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- CN114279775A CN114279775A CN202111638341.8A CN202111638341A CN114279775A CN 114279775 A CN114279775 A CN 114279775A CN 202111638341 A CN202111638341 A CN 202111638341A CN 114279775 A CN114279775 A CN 114279775A
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Abstract
The invention discloses a sampling device for monitoring bioaerosol, which comprises a shell, a sampling part and a liquid filling part, wherein the top of the shell is provided with an air inlet; the sampling part comprises a particle size cutting head, a sampling cup, a fan and an arc-shaped pipeline, and the particle size cutting head is arranged on the air inlet; the sampling cup, the fan and the arc-shaped pipeline are all positioned in the shell, and the tangential air inlet of the sampling cup is communicated with the fan through the arc-shaped pipeline; the top of the sampling cup is provided with an air inlet pipeline and an air outlet pipeline, and the air inlet pipeline is communicated with an air inlet; the liquid filling part comprises a peristaltic pump, a liquid pipeline, a liquid storage bottle, a sample bottle and a waste liquid bottle which are positioned in the shell, and the sampling cup is respectively connected and communicated with the liquid storage bottle, the sample bottle and the waste liquid bottle through the peristaltic pump and the liquid pipeline. The invention can rapidly sample bioaerosol of 0.1-10.0 μm in the environment to form a liquid sample, has more sample processing modes, and can be directly coated on a flat plate for culture or analyzed by instruments such as a fluorescence microscope, a flow cytometer and the like.
Description
Technical Field
The invention relates to the technical field of bioaerosol sampling, in particular to a sampling device for bioaerosol monitoring.
Background
Various microorganisms, also called bioaerosols, exist in the air, and are more than thousands of types, so that the content of bioaerosols in the air becomes a way to evaluate the air quality, on one hand, in the pharmaceutical, cosmetic and food industries, air pollution control is particularly important, production workshops and products of the industries are particularly sensitive to specific bioaerosols pollution, other special production processes also need strict sterile environments, and bioaerosols sampling technology is the most basic evaluation way; on the other hand, in outdoor environments, the environment needs to be monitored and the quality needs to be evaluated by a sampler as well.
At present, the bioaerosol sampling method in the environment mainly comprises a solid impact type method, a filtering type method, an electrostatic sedimentation type method and the like, but all methods have some problems: the solid impact type common six-grade Anderson sampling device has the flow of 28.3L/min, simulates the anatomical structure and the aerodynamic characteristics of the respiratory tract of a human body, respectively collects biological aerosol particles suspended in the air on the surface of a sampling medium (culture dish) according to the size grade by adopting the conventional impact principle, and then cultures the biological aerosol particles, and has the characteristics of high sampling efficiency, wide sampling particle size range and the like, but has the following defects: the culture dishes required for sampling each time are more, the flow rate is small, and in the environment of high-concentration bioaerosol, the number of bacteria sampled by the culture dishes is easy to be excessive and saturated; the filtering type sampling device is used for intercepting microorganisms in air on a filter membrane by filtering the air by using the air extraction device, has the advantages of high sampling efficiency, low environmental temperature and humidity requirements and the like, but is easy to cause the blockage of the filter membrane to influence the flow stability and reduce the sampling effect caused by a secondary treatment mode; the static sedimentation type mainly samples the charged microorganisms in the air through the charged fiber cloth or other conductors, and can keep the activity of the microorganisms to the maximum extent, but the sampling method can only collect the charged microorganisms in the air and cannot represent the actual concentration of the microorganisms in the air; when the traditional liquid cyclone type sampling device is used for sampling low-concentration aerosol, internal sampling liquid is evaporated and dried up in the long-time acquisition process and is difficult to acquire for a long time, and microorganisms in the previous sampling environment can be left in gas circuits such as an air inlet pipeline during acquisition, so that cross contamination is caused;
in addition, in the existing biological aerosol sampling method, when the sampler works in an outdoor environment, outdoor air is different from clean air in a clean room, some non-biological aerosol with the particle size of more than 10 microns exists, and if the sampling result is acquired by the sampler, the sampling result is influenced;
finally, the traditional sampling method needs the culture dish to be placed in an incubator with the constant temperature of 37 ℃, and the result can be obtained after the culture is carried out for 24-48 hours, so that the period is long and the real-time performance is not realized.
Therefore, it is a problem to be solved by those skilled in the art to provide a fast and accurate sampling device for bioaerosol monitoring.
Disclosure of Invention
In view of this, the invention provides a sampling device for bioaerosol monitoring, which can perform long-time liquid phase sampling on bioaerosol of 0.1-10.0 μm in the environment and can perform cleaning of internal pipelines.
In order to achieve the purpose, the invention adopts the following technical scheme:
a sampling device for bioaerosol monitoring, comprising:
the top of the shell is provided with an air inlet;
the sampling part comprises a particle size cutting head, a sampling cup, a fan and an arc-shaped pipeline, and the particle size cutting head is arranged on the air inlet; the sampling cup, the fan and the arc-shaped pipeline are all positioned in the shell, and the tangential air inlet of the sampling cup is communicated with the fan through the arc-shaped pipeline; an air inlet pipeline and an air outlet pipeline are arranged at the top of the sampling cup, and the air inlet pipeline is communicated with the air inlet;
the liquid filling part comprises a peristaltic pump, a liquid pipeline, a liquid storage bottle, a sample bottle and a waste liquid bottle which are positioned inside the shell, and the sampling cup is connected and communicated with the liquid storage bottle, the sample bottle and the waste liquid bottle respectively through the peristaltic pump and the liquid pipeline.
By adopting the scheme, the invention has the beneficial effects that:
1) the sampling cup is connected with the fan through an arc-shaped pipeline, so that the airflow resistance is low, the sampling flow is large, and the rapid sampling can be realized;
2) the carrier is liquid, the sampling solution has more processing modes, can be concentrated or diluted, and can be analyzed by flow cytometry, PCR, immunoassay, fluorescence microscope and other instruments;
3) the particle size cutting head is arranged on the air inlet, so that the aerosol with the particle size of more than 10 micrometers can be effectively cut, and the aerosol is prevented from entering the air inlet pipeline.
Furthermore, the sampling cup further comprises an electric heating device, and the electric heating device is coated on the outer surfaces of the sampling cup and the air inlet pipeline.
The beneficial effect that adopts above-mentioned further technical scheme to produce is, avoids liquid to freeze under the low temperature environment, and equipment can use under the low temperature environment.
The temperature and humidity sensor is mounted at the top of the shell; the temperature and humidity sensor is electrically connected with the controller, and the controller is respectively electrically connected with the electric heating device and the peristaltic pump.
The liquid driving part can automatically fill liquid into the sampling cup before and during sampling under the control of the controller according to signals of the temperature and humidity sensor and the liquid level sensor in the sampling process, so that the liquid is prevented from evaporating and drying up, and long-time continuous sampling is realized.
Further, the particle size cutting head is replaced by an air filter.
The beneficial effect that adopts above-mentioned further technical scheme to produce is, realizes the washing to sampling cup and liquid pipeline.
Further, the sample vial has an external fluid port.
The beneficial effect that adopts above-mentioned further technical scheme to produce is, can connect back level analytical equipment, exports sampling liquid to back level analytical equipment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a front view of a sampling device for bioaerosol monitoring provided by the present invention;
FIG. 2 is a side view of a sampling device for bioaerosol monitoring provided by the present invention;
fig. 3 is a schematic structural diagram of a sampling portion provided by the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-3, the embodiment of the invention discloses a sampling device for bioaerosol monitoring, which comprises a shell 1, a sampling part and a liquid filling part, wherein the top of the shell 1 is provided with an air inlet 11; the sampling part comprises a particle size cutting head 2, a sampling cup 3, a fan 4 and an arc-shaped pipeline 5, and the particle size cutting head 2 is arranged on an air inlet 11; the sampling cup 3, the fan 4 and the arc-shaped pipeline 5 are all positioned in the shell 1, and the tangential air inlet of the sampling cup 3 is communicated with the fan 4 through the arc-shaped pipeline 5; the top of the sampling cup 3 is provided with an air inlet pipeline and an air outlet pipeline, and the air inlet pipeline is communicated with the air inlet 11; in this embodiment, after fan 4 operates, the air current that the air mingled with the microorganism granule gets into the air inlet pipeline by particle size cutting head 2, and the air current is restricted by sampling cup 3 and is made rotary motion, discharges through the exhaust pipe way, and when the air current was made rotary motion, the microorganism granule moved to sampling cup 3 lateral wall under inertial centrifugal force's promotion, and the particle that reaches the lateral wall falls into sampling cup 3 along the wall under the combined action of air current and gravity in, mixes in the sampling liquid. The liquid filling part comprises a peristaltic pump 6, a liquid pipeline 7, a liquid storage bottle 8, a sample bottle 9 and a waste liquid bottle 10 which are positioned inside the shell 1, the sampling cup 3 is respectively connected and communicated with the liquid storage bottle 8, the sample bottle 9 and the waste liquid bottle 10 through the peristaltic pump 6 and the liquid pipeline 7, in the embodiment, the peristaltic pump 6 is provided with a first pump head, a second pump head and a third pump head which are connected in series, the liquid pipeline 7 comprises a first hose, a second hose and a third hose, the first pump head is provided with the first hose, the second pump head is provided with the second hose, the third pump head is provided with the third hose, the sampling cup 3 is connected and communicated with the liquid storage bottle 8 through the first hose, the sampling cup 3 is connected and communicated with the sample bottle 9 through the second hose, and the sampling cup 3 is connected and communicated with the waste liquid bottle 10 through the third hose; in this embodiment, the liquid dosing device of the peristaltic pump 6 allows for precise amounts of liquid.
According to the invention, the sampling cup 3 is connected with the fan 4 through the arc-shaped pipeline 5, so that the airflow resistance is low, the sampling flow is large, and the rapid sampling can be realized; the fan 4 is adopted for sampling, so that the sampling can be carried out quickly in a short time, the sampling device can increase the sampling time to capture enough microorganisms under the condition of low concentration of the microorganisms in the environment, and the sampling liquid can be concentrated, and for the environment with high concentration of the microorganisms, the problem that a culture dish in the traditional sampling mode is invalid due to excessive bacterial colony quantity is not worried about because the sampling liquid is liquid; the carrier is liquid, the sampling solution has more processing modes, can be concentrated or diluted, and can be analyzed by flow cytometry, PCR, immunoassay, fluorescence microscope and other instruments; the particle size cutting head 2 is arranged on the air inlet 11, so that aerosol with the particle size of more than 10 micrometers can be effectively cut, and the aerosol is prevented from entering an air inlet pipeline.
Specifically, the sampling cup further comprises an electric heating device 101, and the electric heating device 101 is coated on the outer surfaces of the sampling cup 3 and the air inlet pipeline.
Specifically, the temperature and humidity sensor device further comprises a temperature and humidity sensor 102 and a controller, wherein the temperature and humidity sensor 102 is installed at the top of the shell 1; the temperature and humidity sensor 102 is electrically connected with the controller, and the controller is respectively electrically connected with the electric heating device 101 and the peristaltic pump 6. Temperature and humidity sensor 102 monitors the environment of sampling to data transfer to the controller in real time, and the controller controls electric heating element 101 according to data, heats the air inlet pipeline, and the gas temperature that gets into in the sampling cup 3 from this is higher than the freezing point, and the sampling liquid can not freeze, and realizes using under the low temperature environment to and control peristaltic pump 6, regularly quantitative filling liquid, thereby prevent that inside sampling liquid from drying up.
Specifically, the particle size cutting head 2 is replaced with an air filter. After the sampling, sampling liquid transmission gets into in the sample bottle 9, sampling cup 3 can feed liquor once more this moment, air cleaner has been changed in air inlet 11 department, open the sampling once more, liquid is at sampling cup 3 internal rotation certain time, wash the gas circuit simultaneously, prevent the inside pollution of gas circuit, in order to reach the cleaning performance, the washing liquid conveys to waste liquid bottle 10, the gas that gets into in the sampling cup 3 this moment is clean gas, no biological aerosol gets into in the sampling liquid, the sampling liquid is equivalent to aseptic liquid this moment, through the air current rotation effect, the cleaning performance to sampling cup 3 and liquid pipeline 7 has been reached.
In particular, the sample vial 9 has an external fluid port 91.
The working process of the invention is as follows:
preparing a sampling solution: if the sample liquid is treated immediately after sampling, pure water can be used as the sampling liquid under the condition of short time; and if the sample solution needs to be stored for a while and then is processed, taking the sampling PBS buffer solution as the sampling solution.
Sampling: with the full sampling liquid in the stock solution bottle 8, put into casing 1, peristaltic pump 6 transmits a plurality of sampling liquid to sampling cup 3 through first hose, and fan 4 begins to operate, samples the surrounding environment, reaches the scheduled time after, and fan 4 stops, and the sampling is ended, and sample liquid in the sampling cup 3 is transmitted to sample bottle 9 by peristaltic pump 6 and second hose again in, takes out sample bottle 9 this moment, can carry out processing on next step to the sample liquid after the sampling.
And (3) cleaning: the cleaning process is identical to the sampling process except that the air inlet 11 is provided with an air filter. An air filter is arranged at an air inlet 11, a peristaltic pump 6 transmits a plurality of sampling liquids to a sampling cup 3 through a first hose, a fan 4 starts to operate, the fan 4 stops operating after 20s, and the sampling liquids in the sampling cup 3 are transmitted to a waste liquid bottle 10 through the peristaltic pump 6 and a third hose.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. A sampling device for bioaerosol monitoring, comprising:
the top of the shell is provided with an air inlet;
the sampling part comprises a particle size cutting head, a sampling cup, a fan and an arc-shaped pipeline, and the particle size cutting head is arranged on the air inlet; the sampling cup, the fan and the arc-shaped pipeline are all positioned in the shell, and the tangential air inlet of the sampling cup is communicated with the fan through the arc-shaped pipeline; an air inlet pipeline and an air outlet pipeline are arranged at the top of the sampling cup, and the air inlet pipeline is communicated with the air inlet;
the liquid filling part comprises a peristaltic pump, a liquid pipeline, a liquid storage bottle, a sample bottle and a waste liquid bottle which are positioned inside the shell, and the sampling cup is connected and communicated with the liquid storage bottle, the sample bottle and the waste liquid bottle respectively through the peristaltic pump and the liquid pipeline.
2. The sampling device of claim 1, further comprising an electrical heating device coated on the outer surface of the sampling cup and the air intake conduit.
3. The sampling device for bioaerosol monitoring of claim 2, further comprising a temperature and humidity sensor and a controller, wherein the temperature and humidity sensor is mounted on the top of the housing; the temperature and humidity sensor is electrically connected with the controller, and the controller is respectively electrically connected with the electric heating device and the peristaltic pump.
4. A sampling device for bioaerosol monitoring according to any of claims 1 to 3, wherein the size cutting head is replaced by an air filter.
5. A sampling device for bioaerosol monitoring as recited in claim 1, wherein the sample vial has an external liquid interface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111638341.8A CN114279775A (en) | 2021-12-29 | 2021-12-29 | Sampling device for monitoring bioaerosol |
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| CN202111638341.8A CN114279775A (en) | 2021-12-29 | 2021-12-29 | Sampling device for monitoring bioaerosol |
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Cited By (3)
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| CN115261213A (en) * | 2022-08-17 | 2022-11-01 | 山东师范大学 | Portable airborne microorganism rapid monitoring device based on automatic control transmission feedback principle |
| CN116793767A (en) * | 2023-05-19 | 2023-09-22 | 夏津新希望六和农牧有限公司 | Active capturing device for African swine fever aerosol and detection method thereof |
| WO2024207602A1 (en) * | 2023-04-06 | 2024-10-10 | 广东中科智能生物技术有限公司 | Aerosol sampler |
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