CN112945837A - Real-time monitoring device for bioaerosol - Google Patents
Real-time monitoring device for bioaerosol Download PDFInfo
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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Abstract
The invention provides a bioaerosol real-time monitoring device, which comprises a main control unit, a display alarm unit, a monitoring unit, a sampling unit, an automatic detection unit and a sterilizing and washing unit, wherein the display alarm unit is used for receiving and displaying real-time data and alarm information processed by the main control unit; the main control unit respectively controls the working states of the display alarm unit, the monitoring unit, the sampling unit, the automatic detection unit and the decontamination unit. The device can be arranged in a vehicle to detect everywhere along with the vehicle when an emergency occurs, and once a contaminated area is monitored, the device can automatically finish early warning, sample and collect the bioaerosol in the surrounding environment, collect the reaction of the sample liquid and the biological species detection card and display and report the monitoring result. The method has the characteristics of strong real-time performance, short identification time, high accuracy, strong automation and low personnel infection risk.
Description
Technical Field
The invention relates to the field of biological monitoring equipment, in particular to a biological aerosol real-time monitoring device.
Background
Along with the innovation of automation, intellectualization, control technology, wireless network technology, sensor technology and other intelligent technologies, the biomedical instruments and devices are developing towards the direction of precision, high efficiency, portability, accuracy, no damage and no contact. Plays an increasingly important role in improving the quality of biotechnology and medical treatment. Compared with the high-speed development of biotechnology, the development of traditional biological detection instruments and technical innovation is obviously delayed. Today, the detection using the conventional biological detection instrument has several problems: (1) the machine is heavy and occupies large space; (2) the operation is complicated, the requirements on the technology and labor force of detection personnel are high, a large amount of manpower is needed, and potential safety hazards are caused to the detection personnel; (3) the precision is not high, and the detection result is not accurate; (4) a plurality of devices are needed for inspection, the detection cost is increased, and non-technical damage is easy to occur when the inspection object is converted among the devices; (5) the detection process and the result presentation are not intuitive enough, and an image amplification device is additionally used; (6) the intelligent degree is low, can't realize automated inspection and original detection control, and the whole journey all needs manual supervision operation. With the development of Chinese economy and the establishment of trade areas, various large conferences, exhibitions and activities are held more and more, the activities are generally short-term, mobile use is required, and meanwhile, aerosol sampling devices are required to be respectively arranged on monitoring equipment and detection equipment, so that the size and the weight are increased, and the manufacturing cost of the system is high.
Disclosure of Invention
The invention provides a real-time bioaerosol monitoring device which can be used for rapidly and accurately monitoring bioaerosol in real time and has low risk.
The technical scheme of the invention is realized as follows: a bioaerosol real-time monitoring device comprises a main control unit, a display alarm unit, a monitoring unit, a sampling unit, an automatic detection unit and a disinfection and washing unit, wherein the display alarm unit is used for receiving and displaying real-time data and alarm information processed by the main control unit; and the main control unit respectively controls the working states of the display alarm unit, the monitoring unit, the sampling unit, the automatic detection unit and the decontamination unit.
Preferably, the monitoring unit consists of a laser particle counter, a photomultiplier, an ultraviolet light induced fluorescence detector, an air pump and an intelligent control module.
Preferably, the sampling unit comprises a fan, a cyclone air duct, a sampling cup and a sampling controller.
Preferably, the automatic detection unit consists of a CCD camera, a fluorescent lamp, a lead screw, a titration head, a titration pump and a test paper board card.
Preferably, the decontamination unit consists of a fluid infusion pump, a decontamination pump, a waste liquid pump, an ultraviolet disinfection lamp, a waste liquid bottle, a raw liquid bottle, a decontamination bottle and a waste liquid collecting port.
Preferably, the display alarm unit consists of a display screen, an indicator light, a key and a buzzer.
Compared with the prior art, the invention has the advantages that: the device is small in size and convenient to transport, can be arranged in a vehicle to detect everywhere along with the vehicle when an emergency situation occurs, and once a contaminated area is monitored, the device can automatically finish early warning, sample and collect the bioaerosol in the surrounding environment, collect the reaction of sample liquid and a biological species detection card and display and report the monitoring result. The method has the characteristics of strong real-time performance, short identification time, high accuracy, strong automation and low personnel infection risk.
Drawings
FIG. 1 is a front view of the on-board bioaerosol reconnaissance system apparatus of the present invention;
FIG. 2 is a rear view of the on-board bioaerosol reconnaissance system apparatus of the present invention;
FIG. 3 is a diagram of a display alarm unit of the present invention;
FIG. 4 is a diagram of the monitoring unit of the present invention;
FIG. 5 is a diagram of a sampling unit composition of the present invention;
FIG. 6 is a diagram of the automatic detection unit according to the present invention;
fig. 7 is a system block diagram of the present invention.
In the figure: 1. a display alarm unit; 2. a monitoring unit; 3. a sampling unit; 4. an automatic detection unit; 5. A decontamination unit; 6. a main control unit; 10. a display screen; 11. an indicator light; 12. pressing a key; 13. a buzzer; 14. a sampling controller; 15. a fan; 16. a cyclonic air duct; 17. a sampling cup; 18. a laser particle counter; 19. an ultraviolet light induced fluorescence detector; 20. a photomultiplier tube; 21. an air pump; 22. an intelligent control module; 23. a screw rod; 24. a CCD camera; 25. a titration head; 26. test paper board card; 28. a waste liquid bottle; 29. a stock solution bottle; 30. washing and disinfecting bottles; 31. a waste liquid pump; 32. a titration pump; 33. a liquid supplementing pump; 34. a decontamination pump; 35. a waste liquid collection port.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
fig. 1 and 2 are schematic structural diagrams of a vehicle-mounted bioaerosol reconnaissance system device of the invention, and fig. 7 is a system block diagram of the invention. As can be seen from the figure, the vehicle-mounted bioaerosol reconnaissance system device comprises a display alarm unit 1, a bioaerosol monitoring unit 2, a sampling unit 3, an automatic detection unit 4, a decontamination unit 5 and a main control unit 6.
As shown in fig. 3, the display alarm unit 1 is composed of a display screen 10, an indicator light 11, a key 12 and a buzzer 13; the display screen 10 is used for displaying real-time data of the bioaerosol concentration of the vehicle-mounted bioaerosol reconnaissance system, displaying the running state, displaying alarm information and displaying parameter setting information; the indicator lamp 11 comprises an operation indicator lamp 11, an alarm indicator lamp 11 and a warning indicator lamp 11, and is used for indicating the operation state, the alarm state, the error state and the system initialization state of the vehicle-mounted bioaerosol reconnaissance system; the key 12 area comprises an upper key, a lower key, a left key, a right key, a confirmation key, a start key and a power key, wherein the upper key, the lower key, the left key, the right key and the confirmation key can be used for setting parameters of the system, the power key is a power supply switch of the whole system, and the start key is the start key 12 after the manual replacement of the system preparation work is completed; the buzzer 13 provides sound prompt for each pre-alarm and after the alarm.
The display alarm unit 1 is connected with the main control unit 6, and is used for displaying real-time data and alarm information processed by the main control unit 6, and simultaneously displaying the working state and setting parameters of the whole system.
As shown in fig. 4, the monitoring unit 2 is composed of a laser particle counter 18, a photomultiplier 20, an ultraviolet light-induced fluorescence detector 19, an air pump 21 and an intelligent control module 22; the air inlet of monitoring unit 2 passes through air pump 21 and links to each other with the small-bore air inlet of system's equipment, and when the bioaerosol concentration in the surrounding environment exceeded the default, the instrument can send the monitoring early warning fast, and the bioaerosol particle diameter range that monitoring unit 2 can monitor is between 0.5um to 10um, detects the lower limit value and can reach 100 ACPLA.
The monitoring unit 2 pumps the bioaerosol in the surrounding environment into the air inlet through the air pump 21; the main control unit 6 is connected with the monitoring unit 2 and is used for receiving, processing and calculating the alarm information and the real-time data of the concentration of the biological particles.
Driven by the air pump 21, the gas flow containing the particles is controlled by a specific gas flow path, passes through the particle cutter, the laser particle counter 18 and is finally discharged out of the system. Wherein the particle cutter is used for filtering large particles with the particle size of more than 10 mu m in the airflow; the laser particle counter 18 realizes the graded counting of the particles in the air flow; the ultraviolet light induced fluorescence detection unit detects the intrinsic fluorescence intensity of the particles in the collection buffer chamber under the excitation of ultraviolet light so as to realize the classification and identification of biological particles and non-biological particles. Through the analysis and calculation of the intelligent control module 22, the monitoring unit 2 can analyze and process the characteristic values of the plant smoke, the engine waste gas, the smoke and the dust in the surrounding environment, can distinguish the difference between the bioaerosol and the interferent, and realizes the accuracy of early warning.
The air pump 21 is started to pump air, the environmental air firstly passes through the laser particle counter 18 to count biological particles and non-biological particles, then enters the ultraviolet light induced fluorescence detector 19 along with the air flow, the detected particles can send out elastic scattered light signals under the excitation of laser, if the detected particles are biological particles, fluorescence can be sent out simultaneously, the scattered light and the fluorescence are separated and then respectively focused on the scattered light detector and the fluorescence detector and converted into electric signals, and the electric signals are calculated, analyzed and processed by the main control unit 6 to obtain the particle size range and the biological particle concentration.
The propagation direction of light is changed by rayleigh scattering, but the wavelength of light is not changed, and the intensity of scattering is inversely proportional to the 4 th power of the wavelength of incident light, see formula a.
In the formula:
e-scattering intensity of light
Lambda-wavelength of light
Alpha-dimensionless particle size parameter
The scattering intensity in terms of mie scattering is proportional to the square of the frequency, see equation B.
E＝αv2………………………………………(B)
In the formula:
e-scattering intensity of light
V-wavelength of light
Alpha-dimensionless particle size parameter
Therefore, the particle size of the particles to be measured is determined based on factors such as the intensity, wavelength, and frequency of the scattered light.
And after the monitoring unit 2 gives a pre-alarm, the main control unit 6 starts the sampling unit 3.
As shown in fig. 2, the sampling unit 3 is composed of a fan 15, a cyclone air duct 16, a sampling cup 17, and a sampling controller 14. The air inlet of the sampling unit 3 is connected with a large-caliber air inlet of system equipment, and the working principle of a cyclone separator is mainly utilized. After entering the sampling cup 17, the microbial aerosol forms an outer vortex from top to bottom along the wall of the cup, and after the vortex descends to the bottom of the cup body, an ascending inner vortex is formed from bottom to top along the axis of the sampling cup 17, and the inner vortex airflow is finally discharged from the exhaust pipe opening. In the sampling process, the particulate matters with larger particle sizes can be thrown to the wall of the sampling cup 17 due to the centrifugal action and are collected in the sampling liquid at the bottom of the sampling cup, and the smaller particulate matters move along with the air flow, change the direction of the air flow at the bottom and flow out to the upper part.
The main control unit 6 is connected with the sampling unit 3 and is used for opening and closing the sampling unit 3, opening and closing the fluid infusion pump 33 and opening and closing the fan 15; when the monitoring unit 2 uploads alarm information, the main control unit 6 immediately starts the sampling unit 3 to sample the bioaerosol of the surrounding environment.
The sampling cup 17 is connected with a liquid supplementing pump 33, a decontamination pump 34 and a drip pump; a fixed amount of sample fluid (e.g., saline) is injected into the sample cup 17 by the fluid infusion pump 33. The effect during sampling is that the airflow with certain wind speed moves according to the double vortex flow spectrum after entering the sampling module, and drives the liquid at the bottom of the cone to rotate to the wall surface to form a water film capable of absorbing microbial particles. The sampling container is made into a transparent piece with smooth inner wall, which is beneficial to cyclone formation on one hand and the survival rate of the collected organisms on the other hand. Through the warning information of the monitoring unit 2, the sampling unit 3 can conveniently and automatically adjust the sampling flow of 100-. The capture efficiency is stable, and the sampling efficiency of the particle with the particle size of 0.5um can reach more than 52 percent. The sampling efficiency of 5um particles can reach more than 98%. After sampling is finished, sample liquid is pumped out through a liquid dropping pump, the sample liquid is titrated to a liquid dropping port of the test paper board card 26 to react with the test paper, the accuracy of the whole titration flow is high, the whole titration flow can be controlled to be 100 +/-10 uL, and after the whole flow is detected, the decontamination liquid is pumped to the sampling cup 17 through the decontamination pump 34 to be disinfected and killed by the sampling cup 17, and a liquid path is disinfected and killed.
An air inlet of a fan 15 is connected with an air inlet of vehicle-mounted bioaerosol reconnaissance system equipment and used for collecting external environment gas, a sampling cup 17 is in threaded connection with a cyclone air duct 16 through a cup opening, a sampling controller 14 is used for controlling the rotating speed of the fan 15, the highest sampling flow can reach 450L/min, and the flow control precision is not more than +/-5%. The sampler separates particles from the gas stream by inertial centrifugal forces acting on the particles of microorganisms in the gas stream during rotation of the gas stream. The main working mechanism of the cyclone dust collector comes from the application of two-phase fluid mechanics in the cyclone dust collector. The air current that contains the microorganism particle gets into the sample thief by tangent line import, the air current that has certain wind speed is after getting into the sample thief, according to the motion of two vortex flow line spectrums, drive cone bottom liquid rotatory to the wall, form the water film that can absorb the microorganism particle, when the air current makes rotary motion, the particle is under inertial centrifugal force's promotion, move to the outer wall, the particle that reaches the outer wall is under the combined action of air current and gravity, fall into the sampling liquid of sampling cup 17 bottom along the wall and collect, and less particulate matter follows the air current motion, change the air current direction at the bottom, flow to the upper portion.
And after the sampling unit 3 is finished, the main control unit 6 starts the automatic detection unit 4.
As shown in fig. 5, the automatic detection unit 4 is composed of a CCD camera 24, a lead screw, a titration head 25, a titration pump 32, a lead screw 23, and a test paper board 26; the CCD camera 24 is arranged on the screw rod 23 with fluorescence, the titration head 25 is arranged on one side of the screw rod 23 close to the camera, and the test paper board card 26 is arranged below the camera and the titration head 25. After the automatic detection unit 4 is started, the main control unit 6 controls the titration pump 32 and the lead screw 23 to start accurate titration and accurate movement, and titration of 16 test paper cards is completed at one time. The titration accuracy for each channel was 100. mu.L. + -. 10. mu.L, with a drop window and a display window for each channel. After the sample solution reacts with the test paper card, the main control unit 6 identifies the display window of each test paper by controlling the combination of the camera and the fluorescent lamp, and whether 16 channels have biological warfare agents or not can be obtained by the reacted test paper under the irradiation of the fluorescent lamp. And finally, the result is uploaded to the main control unit 6, and the main control unit 6 displays and gives out audible and visual alarm prompts on the display alarm unit 1.
After sampling is finished, the main control unit 6 starts the automatic detection unit 4, firstly controls the screw rod 23 to accurately move to the first channel of the test paper board 26, then starts the peristaltic pump to accurately titrate the sample liquid, and the following 15 channels sequentially perform the above-mentioned moving titration. After titration is completed, the screw rod 23 returns to the original position, the sample liquid reacts for 6min, the screw rod 23 is controlled to move to the first channel of the test paper board 26 accurately again, then the camera is started, fluorescence is turned on at the same time, biological test paper can present colors under the irradiation of the fluorescence, the camera identifies the presented colors, and the biological aerosol toxin types are obtained through processing, analyzing and calculating by the DSP processor of the automatic detection unit 4.
The main control unit 6 is connected to the automatic detection unit 4, and is configured to control the automatic detection unit 4 to turn on or off, and receive data from the automatic detection unit 4. After the sampling of the sampling unit 3 is completed, the main control unit 6 closes the sampling unit 3 and immediately starts the automatic detection unit 4, and the automatic detection unit 4 sequentially performs titration of the sample liquid by the titration head 25, movement control of the lead screw, detection control of the CDD, and opening and closing of fluorescence.
The automatic detection unit 4 is connected with the main control unit 6, when the detection of the automatic detection unit 4 is finished, the detection result is immediately sent to the main control unit 6, and the main control unit 6 displays the detection result through the display alarm unit 1.
The decontamination unit 5 consists of a fluid infusion pump 33, a decontamination pump 34, a waste liquid pump 31, an ultraviolet disinfection lamp, a waste liquid bottle 28, a raw liquid bottle 29, a decontamination bottle 30 and a waste liquid collecting port 35; when the display alarm unit 1 displays the detection result, the decontamination unit 5 is automatically started, and the ultraviolet disinfection lamps are sequentially started to sterilize the internal space of the system; starting a decontamination pump 34, and pumping decontamination liquid from the decontamination bottle 30 to perform primary disinfection and flushing on a system liquid path; starting the liquid replenishing pump 33, and extracting the stock solution from the stock solution bottle 29 to perform secondary disinfection and washing on the system liquid path; the generated waste liquid is drawn into the waste liquid pump 31 through the waste liquid collecting port 35 by the waste liquid pump 31. After the decontamination unit 5 is finished, the display alarm unit 1 displays the state of the test paper board, which indicates that the whole process is finished, and an operator replaces the test paper board 26.
The monitoring unit 2 monitors the bioaerosol in the environment, can distinguish the concentration of non-biological particles and biological particles in the air, can resist the smoke, the engine exhaust gas and the plant smoke, and transmits data to the main control unit 6; the main control unit 6 respectively controls the working states of the display alarm unit 1, the monitoring unit 2, the sampling unit 3, the automatic detection unit 4 and the decontamination unit 5, when the concentration of the bioaerosol monitored by the monitoring unit 2 reaches an early warning threshold value, the main control unit 6 controls the display alarm unit 1 to send out an early warning signal, and simultaneously triggers the sampling unit 3 to collect biological particles to be mixed into a sample liquid through a cyclone separation type sampling technology. Then starting an automatic detection module for sampling, adding sample, titrating to a multi-channel chuck filled with detection test paper, and carrying out reaction; and then, the result is quickly read to finish the detection of the type of the biological warfare agent, so that the quick alarm and the toxin type identification of the biological warfare agent are realized. After the alarm detection is finished, the decontamination unit 5 can be automatically started to decontaminate and clean the machine body and the liquid path, so that the manual cleaning of operators is avoided, and the risk of the operators being infected is reduced. The invention can monitor the content of bioaerosol in the atmosphere in real time and carry out early warning, and has the characteristics of strong real-time performance, short identification time, high accuracy, strong automation and low infection risk.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A bioaerosol real-time monitoring device is characterized in that: the device comprises a main control unit, a display alarm unit, a monitoring unit, a sampling unit, an automatic detection unit and a sterilizing and washing unit, wherein the display alarm unit is used for receiving and displaying real-time data and alarm information processed by the main control unit, the monitoring unit is used for monitoring the concentration of bioaerosol in the environment in real time; and the main control unit respectively controls the working states of the display alarm unit, the monitoring unit, the sampling unit, the automatic detection unit and the decontamination unit.
2. The bioaerosol real-time monitoring device of claim 1, wherein: the monitoring unit consists of a laser particle counter, a photomultiplier, an ultraviolet light induced fluorescence detector, an air pump and an intelligent control module.
3. The bioaerosol real-time monitoring device of claim 1, wherein: the sampling unit comprises a fan, a cyclone air duct, a sampling cup and a sampling controller.
4. The bioaerosol real-time monitoring device of claim 1, wherein: the automatic detection unit consists of a CCD camera, a fluorescent lamp, a lead screw, a titration head, a titration pump and a test paper board card.
5. The bioaerosol real-time monitoring device of claim 1, wherein: the decontamination unit consists of a fluid infusion pump, a decontamination pump, a waste liquid pump, an ultraviolet disinfection lamp, a waste liquid bottle, a raw liquid bottle, a decontamination bottle and a waste liquid collecting port.
6. The bioaerosol real-time monitoring device of claim 1, wherein: the display alarm unit consists of a display screen, an indicator light, a key and a buzzer.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113817590A (en) * | 2021-09-07 | 2021-12-21 | 深圳市安保医疗感控科技股份有限公司 | Air microorganism online monitoring equipment and monitoring method |
| CN113916618A (en) * | 2021-09-29 | 2022-01-11 | 中科合肥技术创新工程院 | Online virus monitoring and early warning device |
| CN114279775A (en) * | 2021-12-29 | 2022-04-05 | 军事科学院系统工程研究院卫勤保障技术研究所 | Sampling device for monitoring bioaerosol |
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