CN215179449U

CN215179449U - Real-time monitoring device for bioaerosol

Info

Publication number: CN215179449U
Application number: CN202120291048.8U
Authority: CN
Inventors: 李俊毅; 崔玉舸; 任兴平; 廖浩; 杨红飞; 栾家琪; 陈少涛; 贾磊
Original assignee: Yunnan Security Technology Co ltd
Current assignee: Yunnan Security Technology Co ltd
Priority date: 2021-02-02
Filing date: 2021-02-02
Publication date: 2021-12-14
Anticipated expiration: 2031-02-02

Abstract

The utility model provides a bioaerosol real-time monitoring device, which comprises a main control unit, a display alarm unit for receiving and displaying real-time data and alarm information processed by the main control unit, a monitoring unit for monitoring bioaerosol concentration in an environment in real time, a sampling unit for sampling air and an automatic detection unit for detecting sampled sample liquid; and the main control unit respectively controls the working states of the display alarm unit, the monitoring unit, the sampling unit and the automatic detection unit. The utility model discloses a device can be when taking place emergency, installs in the car, and the vehicle-mounted is scouted everywhere, in case monitor and be infected with the region, the early warning can independently be accomplished to equipment, and the sampling of surrounding environment biological aerosol is collected, collects the reaction of sample liquid and biological species detection card and the demonstration and the report of monitoring result. The method has the characteristics of strong real-time performance, short identification time, high accuracy and strong automation.

Description

Real-time monitoring device for bioaerosol

Technical Field

The utility model relates to a biological monitoring equipment field especially indicates a biological aerosol real-time supervision device.

Background

In recent years, microbial contamination has brought people various adverse health effects and the occurrence of diseases. Bioterrorism attacks and the like are also increasingly threatening human safety. For example, SARS (SARS) in 2003 and a novel coronavirus (COVID-19) in 2019 have great economic impact on society. The main transmission route is through the respiratory tract, and when people sneeze, cough and speak, all the droplets form biological aerosol which is inhaled through the respiratory tract to cause infection. By 12 months 2020, COVID-19 has contributed to over 170 million global deaths. The global economy has been severely impacted. Therefore, a technology and an automatic and intelligent device capable of monitoring the bioaerosol in the surrounding environment in real time are urgently needed.

Along with the innovation of automation, intellectualization, control technology, wireless network technology, sensor technology and other intelligent technologies, the biomedical instruments and devices are developing towards the direction of precision, high efficiency, portability, accuracy, no damage and no contact. Plays an increasingly important role in improving the quality of biotechnology and medical treatment. Compared with the high-speed development of biotechnology, the development of traditional biological detection instruments and technical innovation is obviously delayed. Today, the detection using the conventional biological detection instrument has several problems: (1) the machine is heavy and occupies large space; (2) the operation is complicated, the requirements on the technology and labor force of detection personnel are high, a large amount of manpower is needed, and potential safety hazards are caused to the detection personnel; (3) the precision is not high, and the detection result is not accurate; (4) a plurality of devices are needed for inspection, the detection cost is increased, and non-technical damage is easy to occur when the inspection object is converted among the devices; (5) the detection process and the result presentation are not intuitive enough, and image amplification equipment is additionally used; (6) the intelligent degree is low, can't realize automated inspection and original detection control, and the whole journey all needs manual supervision operation. With the development of Chinese economy and the establishment of trade areas, various large conferences, exhibitions and activities are held more and more, the activities are generally short-term, mobile use is required, and meanwhile, aerosol sampling devices are required to be respectively arranged on monitoring equipment and detection equipment, so that the size and the weight are increased, and the manufacturing cost of the system is high.

SUMMERY OF THE UTILITY MODEL

The utility model provides a biological aerosol real-time supervision device can accurately monitor the aerosol fast in real time, and the risk is low.

The technical scheme of the utility model is realized like this: a bioaerosol real-time monitoring device comprises a main control unit, a display alarm unit, a monitoring unit, a sampling unit and an automatic detection unit, wherein the display alarm unit is used for receiving and displaying real-time data and alarm information processed by the main control unit; and the main control unit respectively controls the working states of the display alarm unit, the monitoring unit, the sampling unit and the automatic detection unit.

Preferably, the monitoring unit consists of a laser particle counter, a photomultiplier, an ultraviolet light induced fluorescence detector, an air pump and an intelligent control module.

Preferably, the sampling unit comprises a fan, a cyclone air duct, a sampling cup and a sampling controller.

Preferably, the automatic detection unit consists of a CCD camera, a fluorescent lamp, a lead screw, a titration head, a titration pump and a test paper board card.

Preferably, the device comprises a display screen, an indicator light, a key and a buzzer.

Compared with the prior art, the utility model has the advantages of: the device is small in size and convenient to transport, can be arranged in a vehicle to detect everywhere along with the vehicle when an emergency situation occurs, and once a contaminated area is monitored, the device can automatically finish early warning, sample and collect the bioaerosol in the surrounding environment, collect the reaction of sample liquid and a biological species detection card and display and report the monitoring result. The method has the characteristics of strong real-time performance, short identification time, high accuracy, strong automation and low personnel infection risk.

Drawings

FIG. 1 is a front view of the on-board bioaerosol reconnaissance system apparatus of the present invention;

FIG. 2 is a rear view of the on-board bioaerosol reconnaissance system apparatus of the present invention;

FIG. 3 is a diagram of the display alarm unit of the present invention;

FIG. 4 is a diagram of the monitoring unit of the present invention;

FIG. 5 is a diagram of the sampling unit of the present invention;

FIG. 6 is a diagram of the automatic detection unit of the present invention;

fig. 7 is a system block diagram of the present invention.

In the figure: 1. a display alarm unit; 2. a monitoring unit; 3. a sampling unit; 4. an automatic detection unit; 6. A main control unit; 10. a display screen; 11. an indicator light; 12. pressing a key; 13. a buzzer; 14. a sampling controller; 15. a fan; 16. a cyclonic air duct; 17. a sampling cup; 18. a laser particle counter; 19. an ultraviolet light induced fluorescence detector; 20. a photomultiplier tube; 21. an air pump; 22. an intelligent control module; 23. a screw rod; 24. a CCD camera; 25. a titration head; 26. test paper board card; 32. a titration pump.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

Example (b):

fig. 1 and fig. 2 are schematic structural views of the vehicle-mounted bioaerosol reconnaissance system apparatus of the present invention, and fig. 6 is a component view of the automatic detection unit of the present invention; fig. 7 is a system block diagram of the present invention. It can be seen from the figure that the utility model discloses on-vehicle bioaerosol reconnaissance system device is including showing alarm unit 1, bioaerosol monitoring unit 2, sampling unit 3, automatic checkout unit 4 and main control unit 6.

As shown in fig. 3, the display alarm unit 1 is composed of a display screen 10, an indicator light 11, a key 12 and a buzzer 13; the display screen 10 is used for displaying real-time data of the bioaerosol concentration of the vehicle-mounted bioaerosol reconnaissance system, displaying the running state, displaying alarm information and displaying parameter setting information; the indicator lamp 11 comprises an operation indicator lamp 11, an alarm indicator lamp 11 and a warning indicator lamp 11, and is used for indicating the operation state, the alarm state, the error state and the system initialization state of the vehicle-mounted bioaerosol reconnaissance system; the key 12 area comprises an upper key, a lower key, a left key, a right key, a confirmation key, a start key and a power key, wherein the upper key, the lower key, the left key, the right key and the confirmation key can be used for setting parameters of the system, the power key is a power supply switch of the whole system, and the start key is the start key 12 after the manual replacement of the system preparation work is completed; the buzzer 13 provides sound prompt for each pre-alarm and after the alarm.

The display alarm unit 1 is connected with the main control unit 6, and is used for displaying real-time data and alarm information processed by the main control unit 6, and simultaneously displaying the working state and setting parameters of the whole system.

As shown in fig. 4, the monitoring unit 2 is composed of a laser particle counter 18, a photomultiplier 20, an ultraviolet light induced fluorescence detector 19, an air pump 21 and an intelligent control module 22; the air inlet of monitoring unit 2 passes through air pump 21 and links to each other with the small-bore air inlet of system's equipment, and when the bioaerosol concentration in the surrounding environment exceeded the default, the instrument can send the monitoring early warning fast, and the bioaerosol particle diameter range that monitoring unit 2 can monitor is between 0.5um to 10um, detects the lower limit value and can reach 100 ACPLA.

The monitoring unit 2 pumps the bioaerosol in the surrounding environment into the air inlet through the air pump 21; the main control unit 6 is connected with the monitoring unit 2 and is used for receiving, processing and calculating the alarm information and the real-time data of the concentration of the biological particles.

Driven by the air pump 21, the gas flow containing the particles is controlled by a specific gas flow path, passes through the particle cutter and the laser particle counter 18 in sequence, and is finally discharged outside the system. Wherein the particle cutter is used for filtering large particles with the particle size of more than 10 mu m in the airflow; the laser particle counter 18 realizes the graded counting of the particles in the air flow; the ultraviolet light induced fluorescence detection unit detects the intrinsic fluorescence intensity of the particles in the collection buffer chamber under the excitation of ultraviolet light so as to realize the classification and identification of the biological particles and the non-biological particles. Through the analysis and calculation of the intelligent control module 22, the monitoring unit 2 can analyze and process the characteristic values of the plant smoke, the engine waste gas, the smoke and the dust in the surrounding environment, can distinguish the difference between the bioaerosol and the interferent, and realizes the accuracy of early warning.

The air pump 21 is started to pump air, the environmental air firstly passes through the laser particle counter 18 to count biological particles and non-biological particles, then enters the ultraviolet light induced fluorescence detector 19 along with the air flow, the detected particles can emit elastic scattered light signals under the excitation of laser, if the detected particles are biological particles, the detected particles can emit fluorescence at the same time, the scattered light and the fluorescence are separated and then respectively focused on the scattered light detector and the fluorescence detector and converted into electric signals, and the electric signals are calculated, analyzed and processed by the main control unit 6 to obtain the particle size range and the biological particle concentration.

The propagation direction of light is changed by rayleigh scattering without changing the wavelength of the light, and the intensity of scattering is inversely proportional to the 4 th power of the wavelength of incident light, see formula a.

In the formula:

e-scattering intensity of light

Lambda-wavelength of light

Alpha-dimensionless particle size parameter

The scattering intensity in terms of mie scattering is proportional to the square of the frequency, see equation B.

E＝αv²………………………………………(B)

In the formula:

e-scattering intensity of light

V-wavelength of light

Alpha-dimensionless particle size parameter

Therefore, the particle size of the particles to be measured is determined based on factors such as the intensity, wavelength, and frequency of the scattered light.

And after the monitoring unit 2 gives a pre-alarm, the main control unit 6 starts the sampling unit 3.

As shown in fig. 2, the sampling unit 3 is composed of a fan 15, a cyclone air duct 16, a sampling cup 17, and a sampling controller 14. The air inlet of the sampling unit 3 is connected with a large-caliber air inlet of system equipment, and the working principle of a cyclone separator is mainly utilized. After entering the sampling cup 17, the microbial aerosol forms an outer vortex from top to bottom along the wall of the cup, and after the vortex descends to the bottom of the cup body, an ascending inner vortex is formed from bottom to top along the axis of the sampling cup 17, and the inner vortex airflow is finally discharged from the exhaust pipe opening. In the sampling process, the particulate matters with larger particle sizes can be thrown to the wall of the sampling cup 17 due to the centrifugal action and are collected in the sampling liquid at the bottom of the sampling cup, and the smaller particulate matters move along with the air flow, change the direction of the air flow at the bottom and flow out to the upper part.

The main control unit 6 is connected with the sampling unit 3 and is used for opening and closing the sampling unit 3, opening and closing the fluid infusion pump 33 and opening and closing the fan 15; when the monitoring unit 2 uploads alarm information, the main control unit 6 immediately starts the sampling unit 3 to sample the bioaerosol of the surrounding environment.

The sampling cup 17 is connected with a liquid supplementing pump 33, a decontamination pump 34 and a drip pump; a fixed amount of sample fluid (e.g., saline) is injected into the sample cup 17 by the fluid infusion pump 33. The effect during sampling is that the airflow with certain wind speed moves according to the double vortex flow spectrum after entering the sampling module, and drives the liquid at the bottom of the cone to rotate to the wall surface to form a water film capable of absorbing microorganism particles. The sampling container is made into a transparent piece with smooth inner wall, which is beneficial to cyclone formation on one hand and the survival rate of the collected organisms on the other hand. Through the pre-alarm information of the monitoring unit 2, the sampling unit 3 can conveniently and automatically adjust the sampling flow of 100 and 450L/min and the sampling time of 1-60 min. The capture efficiency is stable, and the sampling efficiency of the particle with the particle size of 0.5um can reach more than 52 percent. The sampling efficiency of 5um particles can reach more than 98%. After sampling is finished, sample liquid is pumped out through a liquid dropping pump, the sample liquid is titrated to a liquid dropping port of the test paper board card 26 to react with the test paper, the accuracy of the whole titration flow is high and can be controlled within 100 +/-10 uL, and after the whole flow is detected, the decontamination liquid is pumped to the sampling cup 17 through the decontamination pump 34 to sterilize the sampling cup 17, and the liquid path is sterilized.

An air inlet of a fan 15 is connected with an air inlet of vehicle-mounted bioaerosol reconnaissance system equipment and used for collecting external environment gas, a sampling cup 17 is in threaded connection with a cyclone air duct 16 through a cup opening, a sampling controller 14 is used for controlling the rotating speed of the fan 15, the highest sampling flow can reach 450L/min, and the flow control precision is not more than +/-5%. The sampler separates particles from the gas stream by inertial centrifugal forces acting on the particles of microorganisms in the gas stream during rotation of the gas stream. The main working mechanism of the cyclone dust collector comes from the application of two-phase fluid mechanics in the cyclone dust collector. The airflow containing the microorganism particles enters the sampler from a tangential inlet, the airflow with a certain wind speed moves according to a double vortex flow line spectrum after entering the sampler, liquid at the bottom of the cone is driven to rotate to the wall surface to form a water film capable of absorbing the microorganism particles, when the airflow rotates, the particles move towards the outer wall under the pushing of inertial centrifugal force, the particles reaching the outer wall fall into the sampling liquid at the bottom of the sampling cup 17 along the wall surface under the combined action of the airflow and gravity to be collected, and smaller particles move along with the airflow, change the airflow direction at the bottom and flow out to the upper part.

And after the sampling unit 3 is finished, the main control unit 6 starts the automatic detection unit 4.

As shown in fig. 5, the automatic detection unit 4 is composed of a CCD camera 24, a lead screw, a titration head 25, a titration pump 32, a lead screw 23, and a test paper board 26; the CCD camera 24 is arranged on the screw rod 23 with fluorescence, the titration head 25 is arranged on one side of the screw rod 23 close to the camera, and the test paper board card 26 is arranged below the camera and the titration head 25. After the automatic detection unit 4 is started, the main control unit 6 controls the titration pump 32 and the lead screw 23 to start accurate titration and accurate movement, and titration of 16 test paper cards is completed at one time. The titration accuracy for each channel was 100. mu.L. + -. 10. mu.L, with a drop window and a display window for each channel. After the sample solution reacts with the test paper card, the main control unit 6 identifies the display window of each test paper by controlling the combination of the camera and the fluorescent lamp, and whether 16 channels have biological warfare agents or not can be obtained by the reacted test paper under the irradiation of the fluorescent lamp. And finally, the result is uploaded to the main control unit 6, and the main control unit 6 displays and gives out audible and visual alarm prompts on the display alarm unit 1.

After sampling is finished, the main control unit 6 starts the automatic detection unit 4, controls the screw rod 23 to accurately move to the first channel of the test paper board 26, then starts the peristaltic pump to accurately titrate the sample liquid, and sequentially performs the above-mentioned moving titration on the next 15 channels. After titration is completed, the screw rod 23 returns to the original position, the sample liquid reacts for 6min, the screw rod 23 is controlled to move to the first channel of the test paper board 26 accurately again, then the camera is started, meanwhile, fluorescence is turned on, biological test paper can present colors under the irradiation of the fluorescence, the camera identifies the presented colors, and the biological aerosol toxin types are obtained through processing, analyzing and calculating by the DSP processor of the automatic detection unit 4.

The main control unit 6 is connected to the automatic detection unit 4, and is configured to control the automatic detection unit 4 to turn on or off, and receive data from the automatic detection unit 4. After the sampling of the sampling unit 3 is completed, the main control unit 6 closes the sampling unit 3 and immediately starts the automatic detection unit 4, and the automatic detection unit 4 sequentially performs titration of the sample liquid through the titration head 25, movement control of the lead screw, detection control of the CDD, and opening and closing of fluorescence.

The automatic detection unit 4 is connected with the main control unit 6, when the detection of the automatic detection unit 4 is finished, the detection result is immediately sent to the main control unit 6, and the main control unit 6 displays the detection result through the display alarm unit 1.

The monitoring unit 2 monitors the bioaerosol in the environment, can distinguish the concentration of non-biological particles and biological particles in the air, can resist the smoke, the engine exhaust gas and the plant smoke, and transmits data to the main control unit 6; the main control unit 6 respectively controls the working states of the display alarm unit 1, the monitoring unit 2, the sampling unit 3 and the automatic detection unit 4, when the concentration of the bioaerosol monitored by the monitoring unit 2 reaches an early warning threshold value, the main control unit 6 controls the display alarm unit 1 to send out an early warning signal, and simultaneously triggers the sampling unit 3 to collect biological particles to be mixed into a sample liquid in a specific buffer solution through a cyclone separation type sampling technology. Then starting an automatic detection module for sampling, adding sample, titrating to a multi-channel chuck filled with detection test paper, and reacting; and then, the result is quickly read to finish the detection of the type of the biological warfare agent, so that the quick alarm and the toxin type identification of the biological warfare agent are realized. The utility model discloses but bioaerosol content carries out the early warning in real-time supervision atmosphere, has that the real-time nature is strong, the discrimination time is short, the degree of accuracy is high, the strong characteristics of automation.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A bioaerosol real-time monitoring device is characterized in that: the device comprises a main control unit, a display alarm unit, a monitoring unit, a sampling unit, an automatic detection unit and a sterilizing and washing unit, wherein the display alarm unit is used for receiving and displaying real-time data and alarm information processed by the main control unit, the monitoring unit is used for monitoring the concentration of bioaerosol in the environment in real time; and the main control unit respectively controls the working states of the display alarm unit, the monitoring unit, the sampling unit, the automatic detection unit and the decontamination unit.

2. The bioaerosol real-time monitoring device of claim 1, wherein: the monitoring unit consists of a laser particle counter, a photomultiplier, an ultraviolet light induced fluorescence detector, an air pump and an intelligent control module.

3. The bioaerosol real-time monitoring device of claim 1, wherein: the sampling unit comprises a fan, a cyclone air duct, a sampling cup and a sampling controller.

4. The bioaerosol real-time monitoring device of claim 1, wherein: the automatic detection unit consists of a CCD camera, a fluorescent lamp, a lead screw, a titration head, a titration pump and a test paper board card.

5. The bioaerosol real-time monitoring device of claim 1, wherein: the decontamination unit consists of a fluid infusion pump, a decontamination pump, a waste liquid pump, an ultraviolet disinfection lamp, a waste liquid bottle, a raw liquid bottle, a decontamination bottle and a waste liquid collecting port.

6. The bioaerosol real-time monitoring device of claim 1, wherein: the display alarm unit consists of a display screen, an indicator light, a key and a buzzer.