CN105910968B - The early warning of bioaerosol laser monitoring and identification device and method - Google Patents
The early warning of bioaerosol laser monitoring and identification device and method Download PDFInfo
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Abstract
A kind of bioaerosol laser monitoring early warning and identification device, including monitoring unit, sampling unit, discriminating unit and overall control unit；The monitoring unit monitors the bioaerosol in environment and distinguishes abiotic particle and biomone in air, obtains biomone concentration, and is transferred to the overall control unit；The overall control unit controls the working condition of the monitoring unit, sampling unit and discriminating unit respectively, when the bio-aerosol concentration that monitoring unit monitors reaches threshold value of warning, overall control unit issues warning signal, while triggering the sampling unit and starting to acquire bioaerosol sample.The present invention can accurately identify the type of bioaerosol, quickly identify the type of biomone, have the advantages that the identification time is short, accuracy is high.
Description
Technical field
The present invention relates to the monitoring and warning fields of bioaerosol, specifically, being that a kind of can not only carry out biogas
Colloidal sol monitoring and warning, and have the function of the device and method for the bioaerosol type that rapid identification monitors.
Background technique
With the improvement of living standards, the pollution of living environment is got worse, and people increasingly pay close attention to the sky of zone of action
Makings amount is even more focus concerned by people for some pathogenic microorganisms in air.It is high in certain pairs of air quality requirements
Place, such as toilet, operating room, medicament compartment etc., are particularly important the monitoring of air quality.Moreover, certainly
Since being attacked from the U.S. by anthrax within 2001, various countries more pay attention to the monitoring and warning to biological terrorist.Therefore, in order to promote
Into social harmony, improve the quality of living and guarantee country with people's life safety, to bioaerosol carry out real-time monitoring with
Identification is of great significance.
Currently, individual particle laser biology particle counting system has obtained quick hair after Ultraluminescence inductive technology invention
Exhibition, the real-time monitoring to biomone may be implemented using elastic light scattering technology and ultraviolet laser induced fluorescence technology in it.
A kind of first technology " Mobile biological aerosol monitoring alarm system " discloses a kind of bioaerosol monitoring system
It unites (Chinese invention patent, Authorization Notice No.: 102788741 B of CN, authorized announcement date: 2014.12.31).Its course of work
It is: the particle that partial size in aerosol is 2 microns -10 microns is separated first with particle concentrating and separating device, recycles monitoring
The characteristic parameter of aerodynamic size laser measurement system and fluorescence measuring systems detection biomone in unit；Work as biology
When particle characteristics parameter reaches early-warning conditions, then start aerosol acquisition unit and sampled, finally using in detection unit
CCD imaging and signal processing system, detect sample liquid.But first technology has the disadvantage in that
1) formerly technology first carries out particle before monitoring unit to adapt to the lower deficiency of monitoring unit sensitivity
Concentration and separation, cause the microorganism particle not in collection cut size section to cannot be introduced into monitoring unit, fail to report so as to cause system；And
Power consumption is big in the particle concentrator course of work, is unfavorable for portable and outdoor application.
2) formerly aerodynamic size laser measurement system and fluorescence measuring systems are utilized in the monitoring unit of technology, should
System usually requires two kinds of different lasers of wavelength as light source, one is measuring particulate aerodynamic size
Red laser, the second is the third harmonic generation ultraviolet laser of measurement particulate fluorescence.Therefore, the system is multiple with structure
The disadvantages of miscellaneous, bulky, with high costs, is unfavorable for the integrated and miniaturization of whole system.
3) formerly the detection unit of technology detects sample liquid and is examined using CCD imaging and signal processing system
It surveys as a result, still and the undeclared type that can tell biomone.If you need to tell the type of biomone, then the detection
Method usually requires first to grow target organism particle culture, is then based on image procossing and pattern-recognition skill method realizes micro- life
Species differentiate.This method detection cycle is long and is inevitably present certain misclassification rate and reject rate.
Summary of the invention
In order to overcome the shortcomings of above-mentioned first technology, the present invention provides a kind of bioaerosol laser monitoring early warning and identifies
Device and method, it is efficient, quick, accurate to have many advantages, such as, provides for harmful microbe monitoring and warning and identification in air
Powerful guarantee.
Technical solution of the invention is as follows:
A kind of bioaerosol laser monitoring early warning and identification device, including four units: monitoring unit, sampling unit,
Discriminating unit and overall control unit；
The monitoring unit monitors the bioaerosol in environment and distinguishes abiotic particle and biology in air
Particle obtains biomone concentration, and is transferred to the overall control unit；
The overall control unit controls the work shape of the monitoring unit, sampling unit and discriminating unit respectively
State, when the bio-aerosol concentration that monitoring unit monitors reaches threshold value of warning, overall control unit is issued warning signal, together
When the triggering sampling unit start to acquire bioaerosol sample；
The sampling unit is input to the discriminating unit for sampling bioaerosol sample；
The discriminating unit is used for the identification of bioaerosol type, and identification result is transmitted to the overall control
Unit；
The identification knot of the overall control unit combination monitoring unit bio-aerosol concentration measured and discriminating unit
Whether fruit is more than its corresponding alarm threshold value come the concentration for judging target organism particle, if being more than alarm threshold value, issues alarm
Signal.
The threshold value of warning refers to the minimum total of the overall control unit triggering acquisition unit work
Biomone concentration value, the value can be manually set according to demand.
The alarm threshold value refers to the maximum concentration value for allowing target organism particle occur in a certain environment, which can
To be manually set according to demand.
The effect of the monitoring unit be monitor environment in aerosol and distinguish the abiotic particle in air and
Biomone obtains biomone concentration.In order to realize real-time monitoring, preferably, the monitoring unit is single using can be achieved
The individual particle laser biology particle counting system of particle monitoring.
The individual particle laser biology particle counting system utilizes elastic light scattering technology and ultraviolet laser induced fluorescence
Technology realizes to the real-time monitoring of biomone, has the characteristics that high sensitivity, fast and accurately.Elastic light scattering technology refers to:
When the aerosol particle period of the day from 11 p.m. to 1 a.m of the laser irradiation into air can scatter, the wavelength of light and the wavelength phase of irradiation laser are scattered
Together.Particle is bigger, and scattering light is stronger, which is used to measure the partial size of particulate, i.e. optical equivalence partial size.Ultraviolet laser
Induced fluorescence technology refers to: when UV light is to biomone, the organic component inside biomone can issue fluorescence,
Thus fluorescence determines whether biomone.The individual particle laser biology particle counting system is using single laser as light
Source, while the optical equivalence partial size of particulate is detected with fluorescence intensity, only when partial size is in biomone grain
Within the scope of diameter and the particulate of sending fluorescence is just judged as biomone.
The course of work of the individual particle laser biology particle counting system is as described below: the particle in air is with single
The form of grain flows through light sensitive area, generates scattering light.If the particle is biomone while can generate fluorescence, scattered by measurement
The partial size of the available particle of luminous intensity, biomone is determined whether by fluorescence, thus obtains the dense of bioaerosol
Then result is passed to the overall control unit and is handled by degree.
The effect of the sampling unit is to collect aerosol sample, improves the concentration of the bioaerosol in sample, just
In the type for identifying bioaerosol, reduces wrong report and fail to report.Specifically, the sampling unit can be using concentration sampling system
System, or the Concentration Sampling system using big flow.Wherein be concentrated sampling system function be in aerosol in biology grain
Particle concentration in sub- particle size range, is then injected into specific liquid to form sample liquid.
The sampling time of the sampling unit be by the overall control unit strict control, and can be according to reality
Situation is adjusted.
The discriminating unit is a kind of multichannel bioaerosol discriminating unit, for identifying the kind of bioaerosol
Class.Particularly, the discriminating unit uses the multichannel immunochromatography detection system based on up-conversion luminescence principle, work
Principle is: with corresponding antibody specific immune response, the up-conversion luminescence particle-of formation can occur for the checking matter in sample liquid
Tested combination is subsequently fixed on the corresponding position of test strips, to after reaction, be passed using up-conversion luminescence biology
Sensor detects test strips, to obtain checking matter in measuring samples liquid qualitative so that quantitative result, thus sentences
The type of biomone contained in other air.Then, discriminating unit sends analysis result to the overall control unit.More
Mostly, the discriminating unit can also be using other detection systems, such as multichannel nucleic acid detection system etc..
The overall control unit is connected with the monitoring unit, sampling unit, discriminating unit respectively, including four kinds
Function: control, signal transmission and processing, system power supply, the interpretation of result of working condition determine.
The working state control refers to the working condition of the control monitoring unit, sampling unit and discriminating unit.
The system power supply, which refers to, provides electric power to each unit, to keep normal working condition.
The effect that the interpretation of result determines is: the monitoring letter come is transmitted through in conjunction with the monitoring unit and discriminating unit
Breath analyzes the type and concentration of bioaerosol, to determine whether to issue alarm.
The course of work of the early warning of bioaerosol laser monitoring and identification device is as follows: first by the monitoring
Unit monitors the variation of bio-aerosol concentration in environment, the bio-aerosol concentration monitored is transmitted to the overall control
Unit processed is dealt with, and sampling unit and discriminating unit are all in standby mode at this time；When monitoring that bio-aerosol concentration reaches
When threshold value of warning, the overall control unit is issued warning signal, while being triggered the sampling unit and being started acquisition biology
Aerosol sample, after sample collection, the sample input of collection into the discriminating unit；Finally, the identification
Unit completes the identification of bioaerosol type, and identification result is passed to the overall control unit, the overall control
The unit combination monitoring unit bio-aerosol concentration measured processed and the identification result of discriminating unit are to determine whether issue alarm.
Type identification is carried out to bioaerosol using the bioaerosol laser monitoring early warning and identification device
Method, this method comprises the following steps:
1. sample-out count: monitoring unit carries out background values acquisition, obtains in the environment each partial size shelves under normal circumstances
Total particle and biomone background values, and it is transmitted to overall control unit；
2. bio-aerosol concentration monitors: the biomone concentration in monitoring unit real-time monitoring air, it is each what is measured
The biomone concentration of a partial size shelves is transmitted to overall control unit；
3. early warning determines: overall control unit calculates the bio-aerosol concentration after removing background values, and and threshold value of warning
It is compared, when being more than threshold value of warning, then issues warning signal, while triggering collection unit acquires aerosol sample；
4. type identifies: discriminating unit receives the collected aerosol sample of acquisition unit, carries out type identification, and mirror
Other result is transmitted to overall control unit；
5. alarm determines: overall control unit analyzes the identification result from discriminating unit, is in judgement sample
It is no to contain target organism particle:
If containing target organism particle in sample, the particle size range of target organism particle and the partial size of monitoring unit are matched
Shelves range, obtains the concentration of target organism particle, and the concentration for then determining whether there is target organism particle is more than its corresponding report
Alert threshold value issues if being more than alarm threshold value and alarms and provide monitoring analysis report, while sampling unit and discriminating unit stop
Work；
If without target organism particle in sample, return step 1., into next monitoring cycle.
Compared with first technology, technical effect of the invention is as follows:
1) present invention can accurately identify the type of bioaerosol.Discriminating unit of the present invention is using based on upper turn
The multichannel immunochromatographic method or multichannel nucleic acid detection method for changing principle of luminosity can quickly identify the type of biomone, tool
Have the advantages that the identification time is short, accuracy is high.
2) monitoring unit of the invention is individual particle laser biology particle counting system, have accuracy is high, do not need into
Row concentration and separation can carry out the advantages that long-time auto-real-time monitoring.
3) present invention has reasonable structure, and monitoring unit uses single laser as light source, while monitoring aerosol
The optical equivalence partial size and fluorescence intensity of particle, elastic scattering light measurement optical path and fluorescence measurement optical path are total to optical path in structure,
Have the advantages that small in size, structure is simple.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the early warning of bioaerosol laser monitoring and identification device of the present invention.
Fig. 2 is the flow chart of the early warning of bioaerosol laser monitoring and discrimination method of the present invention.
Specific embodiment
Below in conjunction with the attached drawing in this patent embodiment, the technical solution in this patent embodiment is carried out clear
Chu, complete description, it is clear that described embodiment is only a part of embodiment of this patent, rather than whole realities
Apply example.Based on middle embodiment of the invention, those of ordinary skill in the art are obtained under that premise of not paying creative labor
The every other embodiment obtained, all belongs to the protection scope of this patent
Bioaerosol laser monitoring early warning of the present invention and identification device, including four units: monitoring unit 1,
Sampling unit 2, discriminating unit 3 and overall control unit 4.
Contacting between described four units of the early warning of bioaerosol laser monitoring and identification device is as described below: monitoring
The unit 1 biomone concentration monitored is transmitted to overall control unit 4, the working condition of sampling unit 2 and discriminating unit 3 by
Overall control unit 4 determines, sample solution is input to discriminating unit 3 after the sampling of sampling unit 2, discriminating unit 3 is mirror
Other result is transmitted to overall control unit 4.
The effect of the monitoring unit 1 be monitor environment in aerosol and distinguish the abiotic particle in air and
Biomone obtains biomone concentration.In order to realize real-time monitoring, monitoring unit, which uses, can be achieved single that single-particle monitors
Grain laser biology particle counting system.Individual particle laser biology particle counting system utilizes elastic light scattering technology and ultraviolet laser
Induced fluorescence technology realizes to the real-time monitoring of biomone, has the characteristics that high sensitivity, fast and accurately.Elastic light scattering
Technology refers to: when the aerosol particle period of the day from 11 p.m. to 1 a.m of the laser irradiation into air can scatter, scattering the wavelength and irradiation laser of light
Wavelength it is identical.Particle is bigger, and scattering light is stronger, which is used to measure the partial size of particulate, i.e. optical equivalence partial size.
Ultraviolet laser induced fluorescence technology refers to: organic component when on UV light to biomone, inside biomone
Fluorescence can be issued, thus fluorescence determines whether biomone.Only when partial size is in biomone particle size range and is sent out
The particulate of fluorescence is just judged as biomone out.
The course of work of individual particle laser biology particle counting system is as described below: the particle in air is with the shape of individual particle
Formula flows through light sensitive area, generates scattering light.If the particle is biomone while can generate fluorescence, by measuring scattered light intensity
The partial size of available particle, biomone is determined whether by fluorescence, thus obtains the concentration of bioaerosol, then
Result is passed to the overall control unit to be handled.
The effect of the sampling unit 2 is to collect aerosol sample, improves the concentration of the bioaerosol in sample, just
In the type for identifying bioaerosol, reduces wrong report and fail to report.Specifically, the sampling unit 2 can be using concentration sampling
Method, or the Concentration Sampling method using big flow.Wherein be concentrated the method for sampling function be in aerosol in biological
Particle concentration within the scope of particle diameter, is then injected into specific liquid to form sample liquid.Concentrating sample aerosol can be with
Sampler is concentrated by KW-1 type air microbe to realize, sample aerosol solution is then collected and is declined by LL225 hydraulic shock
Bioaerosol sampler is realized.
2 sampling time of sampling unit be by 4 strict control of overall control unit, can be according to practical feelings
Condition is adjusted.
The discriminating unit 3 is the type for identifying bioaerosol.Discriminating unit uses immunochromatographic method, work
Be as principle: bioaerosol particle in sample liquid can and corresponding antibody special immune response occur, thus differentiate
Then the type of bioaerosol in air aerosol is sent to analysis result the overall control unit and integrate and sentences
It is disconnected.Specifically, UPT-M1 biosensor can be used.
The UPT-M1 biosensor is mainly by Systems for optical inspection, two-dimensional motion system, control system and display
System composition.Wherein, Systems for optical inspection mainly completes the excitation and collection to up-conversion luminescence signal on test paper disk, and by light
Signal is converted to electric signal；Two-dimensional motion system mainly realizes the rotation and translational motion of test paper disk；Control system passes through control
Software control two-dimensional motion system, the output electric signal for acquiring Systems for optical inspection, then test paper is obtained by data processing software
The qualitative or quantitative result of ten kinds of checking matters on disk；Display system shows scanning curve and final result on LCD.
The UPT-M1 biosensor, its working principle is that: using ten channel immunity-chromatography test paper discs as detection pair
As measuring samples liquid to be added to the center well of test paper disk first, measuring samples liquid is uniform along the spoke direction of test paper disk
Flowing, during this, the checking matter in sample liquid is immunoreacted with the antibody in ten test strips in test paper disk spoke,
UCP- is detected combination and is subsequently fixed in test strips.To after reaction, using UPT-M1 biosensor to test paper
Disk is detected, and can obtain the qualitative so that quantitative result of ten kinds of checking matters of measuring samples liquid.
The overall control unit 4 is connected in one with the monitoring unit 1, sampling unit 2 and discriminating unit 3 respectively
It rises, in total includes: four parts of control, signal transmission and processing, instrument power supply and interpretation of result judgement of working condition.
The working state control refers to the work shape of the control monitoring unit 1, sampling unit 2 and discriminating unit 3
State.
The instrument power supply, which refers to, provides electric power to the instrument in each unit, keeps normal working condition.
The effect that the interpretation of result determines is: the monitoring come is transmitted through in conjunction with the monitoring unit 1 and discriminating unit 3
Information analyzes the type and concentration of bioaerosol, to determine whether to alarm.
The course of work of the early warning of bioaerosol laser monitoring and identification device is as follows:
The variation for monitoring bio-aerosol concentration in food processing factory's air by the monitoring unit 1 first, monitoring
To bio-aerosol concentration be transmitted to the overall control unit 4 and deal with, sampling unit 2 and discriminating unit 3 be all at this time
It is in standby, when monitoring that bio-aerosol concentration reaches 100/L, the overall control unit 4 issues early warning
Signal, while triggering the sampling unit 2 and starting to acquire bioaerosol sample, after sample collection, the sample of collection
Product are transferred in the discriminating unit 3, and the last discriminating unit 3 completes the identification of bioaerosol type, and will mirror
Other result passes to the overall control unit 4, and the overall control unit 4 is molten in conjunction with the biogas that monitoring unit 1 measures
Gum concentration and the identification result of discriminating unit 3 are to determine whether alarm.
If containing target organism particle Escherichia coli in sample, the particle size range of target organism particle Escherichia coli is matched
The partial size shelves range 0.5um-1.5um of the individual particle laser biology particle counting system of 0.5um-1.5um and monitoring unit, obtains
10/the L of concentration of target organism particle Escherichia coli determines whether that the concentration of target organism particle Escherichia coli is more than that it is corresponding
15/L of alarm threshold value, objective microbe is not above alarm threshold value, is directly entered a monitoring cycle.
Claims (5)
1. a kind of bioaerosol laser monitoring early warning and identification device, which is characterized in that including four units: monitoring unit
(1), sampling unit (2), discriminating unit (3) and overall control unit (4)；
The monitoring unit (1) monitors the bioaerosol in environment and distinguishes the abiotic particle in air and biological grain
Son, the biomone concentration in real-time monitoring air are transmitted to the biomone concentration of each partial size shelves measured described
Overall control unit (4)；
The overall control unit (4) controls the monitoring unit (1), sampling unit (2) and discriminating unit (3) respectively
Working condition, when the biomone concentration that monitoring unit (1) monitors reaches threshold value of warning, overall control unit (4) is issued
Pre-warning signal, while triggering the sampling unit (2) and starting to acquire bioaerosol sample；
The sampling unit (2) is input to the discriminating unit (3) for sampling bioaerosol sample；
The discriminating unit (3) is used for the identification of bioaerosol type, and identification result is transmitted to the overall control
Unit (4)；
The overall control unit (4) determines for alarming, if containing target organism particle in sample, matches target organism
The particle size range of particle and the partial size shelves range of monitoring unit, obtain the concentration of target organism particle, then determine whether there is mesh
The concentration for marking biomone is more than its corresponding alarm threshold value, if being more than alarm threshold value, issues and alarms and provide monitoring analysis
Report, while sampling unit and discriminating unit stop working.
2. bioaerosol laser monitoring early warning according to claim 1 and identification device, which is characterized in that described is pre-
Alert threshold value is minimum total biomone concentration value of the overall control unit triggering sampling unit work, the value root
It is manually set according to demand.
3. bioaerosol laser monitoring early warning according to claim 1 and identification device, which is characterized in that the report
Alert threshold value refers to that the maximum concentration value for allowing target organism particle occur in a certain environment, the value are manually set according to demand.
4. bioaerosol laser monitoring early warning according to claim 1 to 3 and identification device, which is characterized in that institute
The monitoring unit stated is using the individual particle laser biology particle counting system that single-particle monitoring can be achieved.
5. using claim the 1-3 any early warning of bioaerosol laser monitoring and identification device to bioaerosol into
Row type mirror method for distinguishing, which is characterized in that this method comprises the following steps:
1. sample-out count: monitoring unit carries out background values acquisition, obtains in the environment total grain of each partial size shelves under normal circumstances
Son and biomone background values, and it is transmitted to overall control unit；
2. biomone concentration monitor: the biomone concentration in monitoring unit real-time monitoring air, each partial size measured
The biomone concentration of shelves is transmitted to overall control unit；
3. early warning determines: overall control unit calculates the biomone concentration after removing background values, and is compared with threshold value of warning
Compared with, when be more than threshold value of warning, then issue warning signal, at the same trigger sampling unit acquisition aerosol sample；
4. type identifies: discriminating unit receives the collected aerosol sample of sampling unit, carries out type identification, and tie identifying
Fruit is transmitted to overall control unit；
5. alarm determines: overall control unit analyzes the identification result from discriminating unit, determines whether contain in sample
There is target organism particle:
If containing target organism particle in sample, the particle size range of target organism particle and the partial size shelves model of monitoring unit are matched
It encloses, obtains the concentration of target organism particle, the concentration for then determining whether there is target organism particle is more than its corresponding warning level
Value issues if being more than alarm threshold value and alarms and provide monitoring analysis report, while sampling unit and discriminating unit stop work
Make；
If without target organism particle in sample, return step 1., into next monitoring cycle.
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| CN110873678B (en) * | 2018-08-30 | 2022-06-10 | 北京华泰诺安探测技术有限公司 | Biological aerosol particle counting and monitoring equipment and monitoring method thereof |
| CN110411995A (en) * | 2019-07-18 | 2019-11-05 | 上海镭慎光电科技有限公司 | Bioaerosol monitoring device and method based on intrinsic fluorescence bleaching characteristic |
| CN110411921A (en) * | 2019-08-26 | 2019-11-05 | 中国检验检疫科学研究院 | Bioaerosol detection device and detection method |
| CN112014528A (en) * | 2020-09-15 | 2020-12-01 | 北京赫尔默技术有限公司 | Real-time monitoring and identifying instrument and method for air pathogens |
| CN112304834B (en) * | 2020-11-30 | 2024-05-14 | 北京汇丰隆经济技术开发有限公司 | Biological aerosol early warning system |
| CN112649412A (en) * | 2020-12-29 | 2021-04-13 | 北京鼎蓝科技有限公司 | Bio-aerosol early warning device based on ATP bioluminescence technology |
| CN112945837A (en) * | 2021-02-02 | 2021-06-11 | 云南安防科技有限公司 | Real-time monitoring device for bioaerosol |
| CN114459981A (en) * | 2021-12-22 | 2022-05-10 | 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) | Method for detecting bacteria filtering efficiency of mask |
| CN114942208A (en) * | 2022-05-20 | 2022-08-26 | 安徽科创中光科技股份有限公司 | Bioaerosol laser early warning and detection device capable of realizing 3D scanning |
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