CN105910968A - Bioaerosol laser monitoring and early warning and identification device and method - Google Patents
Bioaerosol laser monitoring and early warning and identification device and method Download PDFInfo
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Abstract
The invention relates to a bioaerosol laser monitoring and early warning and identification device, which comprises a monitoring unit, a sampling unit, an identification unit and a total control unit. The monitoring unit monitors bioaerosol in the environment and distinguishes non-bio-particles and bio-particles in the air to obtain a bio-particle concentration, and transmits the bio-particle concentration to the total control unit. The total control unit controls the working state of the monitoring unit, the sampling unit and the identification unit respectively, when the bioaerosol concentration monitored by the monitoring unit reaches an early-warning threshold, the total control unit sends an early-warning signal, and at the same time triggers the sampling unit in terms of starting collection of bioaerosol samples. The device provided by the invention can accurately identify the varieties of bioaerosol, quickly identify the varieties of bio-particles, and has the advantages of short identification time and high accuracy.
Description
Technical field
The present invention relates to the monitoring and warning field of bioaerosol, specifically, be that one is possible not only to
Carry out bioaerosol monitoring and warning, and there is the bioaerosol kind that rapid identification monitors
The device and method of function.
Background technology
Along with the pollution of growth in the living standard, living environment is day by day serious, people increasingly pay close attention to
The air quality of zone of action, for some pathogenic microorganism Jiao the most of concern in air
Point.In the place that some is high to air quality requirements, such as toilet, operating room, medicament compartment
Etc., the monitoring to air quality is particularly important.Moreover, it is subject to calendar year 2001 since the U.S.
Since attacking to anthrax, various countries more pay attention to the monitoring and warning to biological terrorist.Therefore, for
Promote social harmony, improve the quality of living and ensure country and people's life safety, to biology
Aerosol monitor in real time with differentiate significant.
At present, individual particle laser biology particle counting system is after Ultraluminescence inductive technology is invented
Being developed rapidly, it utilizes elastic light scattering technology and Ultra-Violet Laser induced fluorescence technology permissible
Realize the real-time monitoring to biomone.
A kind of biogas is disclosed in first technology " a kind of Mobile biological aerosol monitoring warning system "
(Chinese invention patent, Authorization Notice No.: CN 102788741 B authorize public affairs to colloidal sol monitoring system
Accuse day: 2014.12.31).Its work process is: first with particle concentrating and separating device in aerosol
Particle diameter be the separate particles of 2 microns-10 microns out, recycling monitoring means in air force
Learn particle diameter laser measurement system and the characteristic parameter of fluorescence measuring systems detection biomone；Work as biology
When particle characteristics parameter reaches early-warning conditions, then start aerosol collecting unit and sample, finally
Utilize the CCD imaging in detector unit and signal processing system, sample liquid is detected.But
Have the disadvantage in that in first technology
1) in the deficiency that first technology is relatively low in order to adapt to monitoring means sensitivity, before monitoring means
First particle being carried out concentrating and separating, the microorganism particle caused not in collection cut size section cannot be introduced into
Monitoring means, thus cause system to be failed to report；And power consumption is big in particle concentrator work process, unfavorable
In portable and outdoor application.
2) monitoring means in first technology make use of aerodynamic size laser measurement system and fluorescence
Measurement system, this system typically requires two kinds of different laser instrument of wavelength and surveys as light source, the first
Amount particulate aerodynamic size red laser, its two be measure particulate glimmering
The third harmonic generation ultraviolet laser of light.Therefore, this system has that structure is complicated, bulky, cost
The shortcoming such as high, is unfavorable for the integrated of whole system and miniaturization.
3) detector unit in first technology uses CCD imaging and signal processing system to enter sample liquid
Row detects and obtains testing result, but and the undeclared kind that can tell biomone.As
Need to tell the kind of biomone, then this detection method typically requires and first trains target organism particle
Health preserving is long, is then based on image procossing and pattern recognition skill method realizes microbe species and differentiates.Should
Method detects cycle length and is inevitably present certain misclassification rate and reject rate.
Summary of the invention
In order to overcome the above-mentioned deficiency in first technology, the present invention provides a kind of bioaerosol laser prison
Survey early warning and identification device and method, there is the advantages such as efficient, quick, accurate, for air has
Monitoring and warning and the discriminating of evil microorganism provide powerful guarantee.
The technical solution of the present invention is as follows:
A kind of bioaerosol laser monitoring early warning and identification device, including four unit: monitoring is single
Unit, sampling unit, discriminating unit and overall control unit；
The described bioaerosol in monitoring means monitoring of environmental and distinguish in air abiotic
Particle and biomone, obtain biomone concentration, and be transferred to described overall control unit；
Described overall control unit controls described monitoring means, sampling unit and discriminating list respectively
The duty of unit, when the bio-aerosol concentration that monitoring means monitors reaches threshold value of warning,
Overall control unit sends early warning signal, triggers described sampling unit simultaneously and starts to gather biogas
Sol sample；
Described sampling unit is used for bioaerosol sample of sampling, and is input to described discriminating list
Unit；
Described discriminating unit is for the discriminating of bioaerosol kind, and identification result is passed to institute
The overall control unit stated；
Described overall control unit combines bio-aerosol concentration and the discriminating list that monitoring means records
The identification result of unit judges whether the concentration of target organism particle exceedes its corresponding alarm threshold value,
If exceeding alarm threshold value, then send alarm signal.
Described threshold value of warning refers to the described collecting unit work described in overall control unit triggering
The minimum total biomone concentration value made, this value can be manually set according to demand.
Described alarm threshold value refers to allow the most highly concentrated of target organism particle occur in a certain environment
Angle value, this value can be manually set according to demand.
The effect of described monitoring means be the aerosol in monitoring of environmental and distinguish in air non-
Biomone and biomone, obtain biomone concentration.In order to realize monitoring in real time, it is preferred that
Described monitoring means uses the individual particle laser biology particle counting system that can realize single-particle monitoring
System.
Described individual particle laser biology particle counting system utilizes elastic light scattering technology and ultraviolet to swash
Light induced fluorescence technology realizes the real-time monitoring to biomone, has highly sensitive, quick, accurate
True feature.Elastic light scattering technology refers to: the aerosol particle period of the day from 11 p.m. to 1 a.m in laser is irradiated to air
Can scatter, the wavelength of scattered light is identical with the wavelength of irradiation laser.The biggest scattered light of granule
The strongest, this technology is used for measuring the particle diameter of particulate, i.e. optical equivalence particle diameter.Ultra-Violet Laser
Induced fluorescence technology refers to: when UV light to biomone, having within biomone
Machine composition can send fluorescence, and thus fluorescence determines whether biomone.Described individual particle swashs
Photo bio particle counting system with single laser instrument as light source, the optics etc. to particulate simultaneously
Effect particle diameter detect with fluorescence intensity, only in particle diameter is in biomone particle size range and
The particulate going out fluorescence is just judged as biomone.
The work process of described individual particle laser biology particle counting system is as described below: in air
Particle flow through light sensitive area with the form of individual particle, produce scattered light.If this particle is biological grain
Son can produce fluorescence simultaneously, can obtain the particle diameter of particle by measuring scattered light intensity, by glimmering
Light determines whether biomone, thus obtains the concentration of bioaerosol, then result is passed
Process to described overall control unit.
The effect of described sampling unit is to collect aerosol sample, improves the biogas in sample molten
The concentration of glue, it is simple to differentiate the kind of bioaerosol, reduces wrong report and fails to report.Specifically, institute
The sampling unit stated can use concentration sampling system, or uses the Concentration Sampling system of big flow.
The function wherein concentrating sampling system is the particle being in aerosol in biomone particle size range
Concentrate, be then injected in specific liquid forming sample liquid.
The sampling time of described sampling unit is strictly to be controlled by described overall control unit,
And can be adjusted according to practical situation.
Described discriminating unit is a kind of multichannel bioaerosol discriminating unit, is used for differentiating biology
Aerocolloidal kind.Especially, described discriminating unit uses many based on up-conversion luminescence principle
Passage immunochromatography detecting system, its operation principle is: the checking matter in sample liquid can be with correspondence
Antibody generation specific immune response, the up-conversion luminescence granule of formation-checking matter conjugate finally quilt
It is fixed on the relevant position of test strips, after question response terminates, uses up-conversion luminescence bio-sensing
Test strips is detected by device, thus draw the qualitative of checking matter in measuring samples liquid to such an extent as to quantitatively
As a result, the kind of biomone contained in air is thus differentiated.Then, discriminating unit is dividing
Analysis result delivers to described overall control unit.More, described discriminating unit can also use
Other detecting systems, such as multichannel nucleic acid detection system etc..
Described overall control unit respectively with described monitoring means, sampling unit, discriminating unit
Be connected, including four kinds of functions: the control of duty, signal transmission and process, system power supply,
Interpretation of result judges.
Described working state control accuses monitoring means, sampling unit and the discriminating unit described in system
Duty.
Described system power supply refers to provide electric power, to keep normal duty to unit.
The effect that described interpretation of result judges is: monitoring means and discriminating unit described in combination pass
Kind and the concentration of bioaerosol are analyzed by the monitoring information come over, and determine whether to send
Report to the police.
Described bioaerosol laser monitoring early warning is as follows with the work process of identification device: first
By the change of bio-aerosol concentration in described monitoring means monitoring of environmental, the biology monitored
Aerosol concentration is sent to described overall control unit and deals with, and now sampling unit and discriminating are single
Unit is all in holding state；When monitoring bio-aerosol concentration and reaching threshold value of warning, described
Overall control unit sends early warning signal, triggers described sampling unit simultaneously and starts to gather biogas
Sol sample, after sample collection, is input to the sample collected in described discriminating unit；
Finally, described discriminating unit completes the discriminating of bioaerosol kind, and is passed to by identification result
Described overall control unit, described overall control unit combines the biogas that monitoring means records
The identification result of collosol concentration and discriminating unit judges whether to send warning.
Bioaerosol is carried out by the bioaerosol laser monitoring early warning described in utilization with identification device
Kind mirror method for distinguishing, the method comprises the steps:
1. sample-out count: monitoring means carries out background values collection, obtains in this environment under normal circumstances
Total particle of each particle diameter shelves and biomone background values, and it is sent to overall control unit；
2. bio-aerosol concentration monitoring: monitoring means monitors the biomone concentration in air in real time,
The biomone concentration of each particle diameter shelves recorded is sent to overall control unit；
3. early warning judges: overall control unit calculates the bio-aerosol concentration after removing background values,
And compare with threshold value of warning, when exceeding threshold value of warning, then send early warning signal, trigger simultaneously
Collecting unit gathers aerosol sample；
4. kind differentiates: discriminating unit receives the aerosol sample that collecting unit collects, and plants
Class differentiates, and identification result is sent to overall control unit；
5. report to the police judgement: the identification result from discriminating unit is analyzed by overall control unit,
Judge whether sample contains target organism particle:
If containing target organism particle in sample, then mating particle size range and the prison of target organism particle
Survey the particle diameter shelves scope of unit, obtain the concentration of target organism particle, then determined whether target
The concentration of biomone exceedes its corresponding alarm threshold value, if exceeding alarm threshold value, then sends warning
And providing monitoring analysis report, sampling unit and discriminating unit quit work simultaneously；
If driftlessness biomone in sample, then return step 1., enter the next monitoring cycle.
Compared with in first technology, the technique effect of the present invention is as follows:
1) present invention can accurately identify the kind of bioaerosol.Discriminating unit of the present invention uses
Be multichannel immunochromatographic method based on up-conversion luminescence principle or multichannel nucleic acid detection method, can
Quickly to differentiate the kind of biomone, there is the advantage that the discriminating time is short, accuracy is high.
2) monitoring means of the present invention is individual particle laser biology particle counting system, has degree of accuracy
High, be made without concentrating and separating, the advantages such as long-time auto-real-time monitoring can be carried out.
3) present invention has rational structure, and the monitoring means single laser instrument of employing is as light source, same
Time the monitoring optical equivalence particle diameter of particulate and fluorescence intensity, structurally elastic scattering flash ranging
Amount light path and fluorescence measurement light path altogether light path, has that volume is little, the advantage of simple in construction.
Accompanying drawing explanation
Fig. 1 is the structural representation of bioaerosol laser monitoring early warning of the present invention and identification device.
Fig. 2 is the flow chart of bioaerosol laser monitoring early warning of the present invention and discrimination method.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this patent embodiment, to the technology in this patent embodiment
Scheme carries out clear, complete description, it is clear that described embodiment is only this patent one
Some embodiments rather than whole embodiments.Middle embodiment based on the present invention, ability
The every other enforcement that territory those of ordinary skill is obtained under not paying creative work premise
Example, broadly falls into the protection domain of this patent
Bioaerosol laser monitoring early warning of the present invention and identification device, including four unit:
Monitoring means 1, sampling unit 2, discriminating unit 3 and overall control unit 4.
Between described bioaerosol laser monitoring early warning and four unit of identification device contact as
Lower described: the biomone concentration that monitoring means 1 monitors passes to overall control unit 4, adopts
The duty of sample unit 2 and discriminating unit 3 is determined by overall control unit 4, sampling unit 2
After sampling, sample solution being input to discriminating unit 3,3 identification results of discriminating unit are passed to
Overall control unit 4.
The effect of described monitoring means 1 is the aerosol in monitoring of environmental and distinguishes in air
Abiotic particle and biomone, obtain biomone concentration.In order to realize monitoring in real time, monitoring
Unit uses the individual particle laser biology particle counting system that can realize single-particle monitoring.Individual particle swashs
Photo bio particle counting system utilizes elastic light scattering technology and Ultra-Violet Laser induced fluorescence technology to realize
Real-time monitoring to biomone, has highly sensitive, feature fast and accurately.Elastic optical dissipates
The technology of penetrating refers to: the aerosol particle period of the day from 11 p.m. to 1 a.m in laser is irradiated to air can scatter, scattered light
Wavelength identical with the wavelength of irradiation laser.The biggest scattered light of granule is the strongest, and this technology is used for surveying
The particle diameter of amount particulate, i.e. optical equivalence particle diameter.Ultra-Violet Laser induced fluorescence technology refers to:
When on UV light to biomone, the organic component within biomone can send fluorescence,
Thus fluorescence determines whether biomone.Only in particle diameter is in biomone particle size range
And the particulate sending fluorescence is just judged as biomone.
The work process of individual particle laser biology particle counting system is as described below: the granule in air
Flow through light sensitive area with the form of individual particle, produce scattered light.If this particle is biomone
Fluorescence can be produced, the particle diameter of particle can be obtained by measuring scattered light intensity, sentenced by fluorescence
Whether fixed is biomone, thus obtains the concentration of bioaerosol, then result is passed to described
Overall control unit process.
The effect of described sampling unit 2 is to collect aerosol sample, improves the biogas in sample
The concentration of colloidal sol, it is simple to differentiate the kind of bioaerosol, reduces wrong report and fails to report.Specifically,
Described sampling unit 2 can use the concentration method of sampling, or uses the Concentration Sampling of big flow
Method.The function wherein concentrating the method for sampling is being in aerosol in biomone particle size range
Particle concentrate, be then injected in specific liquid forming sample liquid.Concentrating sample aerosol can
To be realized by KW-1 type air microbe concentration sampler, then collect sample aerosol solution
Realized by the LL225 hydraulic shock bioaerosol sampler that declines.
Described sampling unit 2 sampling time is strictly to be controlled by described overall control unit 4,
Can be adjusted according to practical situation.
Described discriminating unit 3 is used to differentiate the kind of bioaerosol.Discriminating unit uses immunity
Chromatography, its operation principle is: the bioaerosol particle in sample liquid can be sent out with corresponding antibody
Raw special immunoreation, thus differentiates the kind of bioaerosol in air aerosol, then
Analysis result is delivered to described overall control unit and carries out comprehensive descision.Specifically, can use
UPT-M1 biosensor.
Described UPT-M1 biosensor mainly by Systems for optical inspection, two-dimensional motion system,
Control system and display system composition.Wherein, Systems for optical inspection mainly completes on test paper disk
Change exciting and collecting of luminous signal, and convert optical signals to the signal of telecommunication；Two-dimensional motion system
Mainly realize rotation and the translational motion of test paper disk；Control system controls two maintenance and operations by controlling software
Dynamic system, the output signal of telecommunication of collection Systems for optical inspection, then draw examination by data processing software
The qualitative or quantitative result of ten kinds of checking matters on paper disc；Display system is by scanning curve and final result
Display is on LCD.
Described UPT-M1 biosensor, its operation principle is: with ten passage immune chromatography test papers
Measuring samples liquid, as detection object, is first added to the center well of test paper disk, treats sample by dish
Product liquid is along the spoke direction Uniform Flow of test paper disk, during this, the checking matter in sample liquid with
The antibody generation immunoreation in ten test strips on test paper disk spoke, UCP-checking matter conjugate
It is subsequently fixed in test strips.After question response terminates, use UPT-M1 biosensor to examination
Paper disc detects, and can draw the qualitative of ten kinds of checking matters of measuring samples liquid to such an extent as to quantitative result.
Described overall control unit 4 respectively with described monitoring means 1, sampling unit 2 and differentiate
Unit 3 connects together, and altogether includes: the control of duty, signal transmission and process, instrument
Power supply and interpretation of result judge four parts.
Described working state control is accused the monitoring means 1 described in system, sampling unit 2 and differentiates single
The duty of unit 3.
Described instrument is powered and is referred to provide electric power to the instrument in unit, and keep working normally shape
State.
The effect that described interpretation of result judges is: the monitoring means 1 described in combination and discriminating unit
Kind and the concentration of bioaerosol are analyzed by 3 monitoring informations being transmitted through, and determine whether
Report to the police.
Described bioaerosol laser monitoring early warning is as follows with the work process of identification device:
First bio-aerosol concentration in food processing factory's air is monitored by described monitoring means 1
Change, is sent to described overall control unit 4 the bio-aerosol concentration monitored and deals with,
Now sampling unit 2 and discriminating unit 3 are all in holding state, dense when monitoring bioaerosol
When degree reaches 100/L, described overall control unit 4 sends early warning signal, triggers institute simultaneously
The sampling unit 2 stated starts to gather bioaerosol sample, after sample collection, collect
Sample is transferred in described discriminating unit 3, and it is molten that last described discriminating unit 3 completes biogas
The discriminating of glue kind, and identification result is passed to described overall control unit 4, described is overall
Control unit 4 combines bio-aerosol concentration and the discriminating of discriminating unit 3 that monitoring means 1 records
Result judges whether to report to the police.
If containing target organism particle escherichia coli in sample, then coupling target organism particle large intestine bar
Particle size range 0.5um-1.5um of bacterium and the individual particle laser biology particle counting system of monitoring means
Particle diameter shelves scope 0.5um-1.5um, obtain target organism particle 10/L of colibacillary concentration,
Determine whether that the colibacillary concentration of target organism particle exceedes its corresponding 15/L of alarm threshold value,
Objective microbe is not above alarm threshold value, is directly entered a monitoring cycle.
Claims (5)
1. a bioaerosol laser monitoring early warning and identification device, it is characterised in that include four
Individual unit: monitoring means (1), sampling unit (2), discriminating unit (3) and overall control unit
(4)；
The described bioaerosol in monitoring means (1) monitoring of environmental and distinguish in air non-
Biomone and biomone, obtain biomone concentration, and be transferred to described overall control list
Unit (4)；
Described overall control unit (4) controls described monitoring means (1), sampling unit respectively
(2) and the duty of discriminating unit (3), the biogas monitored when monitoring means (1) is molten
When gum concentration reaches threshold value of warning, overall control unit (4) sends early warning signal, triggers institute simultaneously
The sampling unit (2) stated starts to gather bioaerosol sample；
Described sampling unit (2) is used for bioaerosol sample of sampling, and is input to described mirror
Other unit (3)；
Described discriminating unit (3) is for the discriminating of bioaerosol kind, and identification result is passed
To described overall control unit (4)；
Described overall control unit (4) combines bio-aerosol concentration and the mirror that monitoring means records
The identification result of other unit judges whether the concentration of target organism particle exceedes its corresponding warning level
Value, if exceeding alarm threshold value, then sends alarm signal.
Bioaerosol laser monitoring early warning the most according to claim 1 and identification device, its
Being characterised by, described threshold value of warning is the collecting unit described in described overall control unit triggers
Minimum total biomone concentration value of work, this value can be manually set according to demand.
Bioaerosol laser monitoring early warning the most according to claim 1 and identification device, its
Being characterised by, described alarm threshold value refers to allow target organism particle occur in a certain environment
Maximum concentration value, this value can be manually set according to demand.
4. according to the arbitrary described bioaerosol laser monitoring early warning of claim 1-3 and discriminating dress
Put, it is characterised in that described monitoring means uses the individual particle laser that can realize single-particle monitoring
Biomone number system.
5. utilize the arbitrary described bioaerosol laser monitoring early warning of claim 1-3 and differentiate dress
Put and bioaerosol is carried out kind mirror method for distinguishing, it is characterised in that the method includes walking as follows
Rapid:
1. sample-out count: monitoring means carries out background values collection, obtains in this environment under normal circumstances
Total particle of each particle diameter shelves and biomone background values, and it is sent to overall control unit；
2. bio-aerosol concentration monitoring: monitoring means monitors the biomone concentration in air in real time,
The biomone concentration of each particle diameter shelves recorded is sent to overall control unit；
3. early warning judges: overall control unit calculates the bio-aerosol concentration after removing background values,
And compare with threshold value of warning, when exceeding threshold value of warning, then send early warning signal, trigger simultaneously
Collecting unit gathers aerosol sample；
4. kind differentiates: discriminating unit receives the aerosol sample that collecting unit collects, and plants
Class differentiates, and identification result is sent to overall control unit；
5. report to the police judgement: the identification result from discriminating unit is analyzed by overall control unit,
Judge whether sample contains target organism particle:
If containing target organism particle in sample, then mating particle size range and the prison of target organism particle
Survey the particle diameter shelves scope of unit, obtain the concentration of target organism particle, then determined whether target
The concentration of biomone exceedes its corresponding alarm threshold value, if exceeding alarm threshold value, then sends warning
And providing monitoring analysis report, sampling unit and discriminating unit quit work simultaneously；
If driftlessness biomone in sample, then return step 1., enter the next monitoring cycle.
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