CN105838581B - A kind of animal ovary egg mother cell separates and collects device - Google Patents
A kind of animal ovary egg mother cell separates and collects device Download PDFInfo
- Publication number
- CN105838581B CN105838581B CN201610313218.1A CN201610313218A CN105838581B CN 105838581 B CN105838581 B CN 105838581B CN 201610313218 A CN201610313218 A CN 201610313218A CN 105838581 B CN105838581 B CN 105838581B
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- China
- Prior art keywords
- egg
- egg apparatus
- hold
- support plate
- mother cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 210000001672 ovary Anatomy 0.000 title claims abstract description 28
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 28
- 235000019994 cava Nutrition 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 4
- 210000000287 oocyte Anatomy 0.000 abstract description 5
- 210000001519 tissue Anatomy 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 210000002394 ovarian follicle Anatomy 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 210000001733 follicular fluid Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000000059 patterning Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of animal ovary egg mother cell to separate and collect device, including holding egg apparatus, three parts of support plate and collection ware, described hold by holding egg apparatus push rod push up and down inside egg apparatus, it is described hold egg apparatus bottom be provided with hold egg apparatus chuck, described holding egg apparatus chuck and hold egg apparatus push rod is connected as a single entity, described hold below egg apparatus is support plate, the support plate is the toroidal to cave inward, it caves inward to form placement mouth, the placement mouth is vacantly connected one by connector and adjustable holds knife ring, adjustable hold is evenly arranged cutter on knife ring, the ware of collecting is arranged on the egg mother cell collected below support plate from ovary.The present invention has the advantages that oocyte collection is time saving and energy saving, and high efficiency is inexpensive, easy to use.
Description
Technical field
The present invention relates to Cell collection device equipment, particularly a kind of animal ovary egg mother cell separates and collects device.
Background technology
The egg mother cell of animal is also known as ovum, and ovum is a new living individual genesis and development with the combination of sperm
Premise and basis.With the development of modern life science, egg mother cell is rearing new variety, transgenic breeding and breed improvement etc.
The important materials of aspect.The acquisition of animal oocyte is usually the animal ovary tissue provided from slaughterhouse, and animal is slaughtered
Animal ovary tissue can not be eaten after government official, and egg mother cell is separated and collected if it goes without doing, and can often go out of use processing.From slaughterhouse from
Substantial amounts of egg mother cell can be isolated in body animal ovary tissue and is used for rearing new variety, transgenic breeding and breed improvement etc.
Related work.
At present, the conventional method of egg mother cell is separated out of slaughterhouse in vitro animal ovary tissue to be had：1 suction method, suction
Method is exactly gently to be pierced into the ovarian follicle of ovary tissue to draw egg mother cell and liquor folliculi using syringe needle, often inhales 2~5 ovum
After bubble, the liquid in syringe is slowly injected in ovoscopy cup, in case checking.2 patterning methods, cut in length and breadth by razor blade with hand
Ovary, by the liquid of recovery be placed in ovoscopy cup it is static after, put ovoscopy cup and pick egg mother cell under microscope, the program is taken
When.3 stripping methods, with Sterile ophthalmic scissors or blade, from around ovarian follicle, it is separated with surrounding tissue, removes complete circle
Ovarian follicle, it is put into the plate added with nutrient solution, cuts ovarian follicle, liquor folliculi flows out from ovarian follicle and carries egg mother cell, the program
It is complicated and less efficient.When gathering Oocytes, in the ovary substantial amounts and in vitro ovary sent due to slaughterhouse
Egg mother cell must gather out to improve the quality of egg mother cell as early as possible, so it is to solve to improve collecting efficiency and acquisition quality
Egg mother cell separates and collects the key of process.At present, in actual applications also not dedicated for animal ovary egg mother cell point
From collection device, according to production practices, we, which design, has invented egg mother cell in a kind of in vitro ovary of animal and separates and collects device,
It is time saving and energy saving that the device has, high efficiency, low cost, it is easy to use the advantages that.
The content of the invention
It is an object of the invention to provide a kind of animal ovary egg mother cell to separate and collect device.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of animal ovary egg mother cell separates and collects device, including holds egg apparatus, support plate and collect three parts of ware, institute
State hold inside egg apparatus by hold egg apparatus push rod carry out up and down push, it is described hold egg apparatus bottom be provided with hold egg apparatus chuck, it is described
Hold egg apparatus chuck and hold egg apparatus push rod and be connected as a single entity, described hold below egg apparatus is support plate, and the support plate is to cave inward
Toroidal, it caves inward to form placement mouth, it is described placement mouth vacantly connected by connector one it is adjustable hold knife ring,
Adjustable hold is evenly arranged cutter on knife ring, the ware of collecting is arranged on the ovum collected below support plate from ovary
Mother cell.
The connector is retractable material, and the adjustable knife ring of holding relies on connector telescopic adjustment size.
The cutter have multiple.
Beneficial effects of the present invention：The present invention has oocyte collection time saving and energy saving, high efficiency, low cost, uses letter
Folk prescription just the advantages that.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention.
Fig. 2 is support plate structure schematic diagram of the present invention.
Marked in figure, 1- holds egg apparatus push rod, and 2- holds egg apparatus, and 3- holds egg apparatus chuck, 4- support plates, and 5- collects ware, and 6- is put
Mouth is put, 7- is adjustable to hold knife ring, 8- cutter, 9- connectors.
Embodiment
The present invention is described in further detail with specific embodiment below in conjunction with the accompanying drawings.
As shown in figure 1, it is an object of the invention to provide a kind of animal ovary egg mother cell to separate and collect device, including hold
5 three egg apparatus 2, support plate 4 and collection ware parts, described hold by holding egg apparatus push rod 1 push up and down inside egg apparatus 2,
Provided with egg apparatus chuck 3 is held, described holding egg apparatus chuck 3 and hold egg apparatus push rod 1 is connected as a single entity, described for the bottom of egg apparatus 2 of holding
It is support plate 4 to hold the lower section of egg apparatus 2, and the support plate 4 is the toroidal to cave inward, and it, which caves inward to be formed, places mouth 6, institute
State placement mouth 6 and hold knife ring 7 by the way that the hanging connection one of connector 9 is adjustable, adjustable hold is evenly arranged cutting on knife ring 4
Knife 8, the ware 5 of collecting are arranged on egg mother cell of the lower section of support plate 4 collection from ovary, and the connector 9 is scalable
Material, the adjustable knife ring 7 of holding rely on the telescopic adjustment size of connector 9, and the cutter 8 have multiple.
In use, support plate 4 is placed on oocytes collection ware 5, hand grips egg apparatus 2, with holding the chuck of egg apparatus 2 ovum
The ovary that nest is put into support plate 4 places mouth 6, and pressing push rod 1 is made ovary hold knife ring 7 by adjustable, completed using cutter 8
Ovary is cut, and after ovary cutting, takes collection ware 5 to pick egg mother cell under the microscope.
Above content is only to structure example of the present invention and explanation, affiliated those skilled in the art couple
Described specific embodiment is made various modifications or supplement or substituted using similar mode, without departing from invention
Structure surmounts scope defined in the claims, all should belong to protection scope of the present invention.
Claims (3)
1. a kind of animal ovary egg mother cell separates and collects device, including holds egg apparatus (2), support plate (4) and collect (5) three, ware
Part, it is characterised in that the egg apparatus (2) of holding is internal by holding egg apparatus push rod (1) progress push up and down, described to hold egg apparatus
(2) bottom is provided with and holds egg apparatus chuck (3), and described holding egg apparatus chuck (3) and hold egg apparatus push rod (1) is connected as a single entity, described to hold
It is support plate (4) below egg apparatus (2), the support plate (4) is the toroidal to cave inward, and it caves inward to form placement mouth
(6), vacantly connection one is adjustable holds knife ring (7) by connector (9) for the placement mouth (6), described adjustable to hold knife ring (7)
On be evenly arranged cutter (8), the ware (5) of collecting is arranged on the egg mother cell collected below support plate (4) from ovary.
2. a kind of animal ovary egg mother cell separates and collects device according to claim 1, it is characterised in that the connector
(9) it is retractable material, the adjustable knife ring (7) of holding relies on connector (9) telescopic adjustment size.
3. a kind of animal ovary egg mother cell separates and collects device according to claim 1, it is characterised in that the cutter
(8) have multiple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610313218.1A CN105838581B (en) | 2016-05-11 | 2016-05-11 | A kind of animal ovary egg mother cell separates and collects device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610313218.1A CN105838581B (en) | 2016-05-11 | 2016-05-11 | A kind of animal ovary egg mother cell separates and collects device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105838581A CN105838581A (en) | 2016-08-10 |
| CN105838581B true CN105838581B (en) | 2017-11-10 |
Family
ID=56591944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610313218.1A Expired - Fee Related CN105838581B (en) | 2016-05-11 | 2016-05-11 | A kind of animal ovary egg mother cell separates and collects device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105838581B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES1214345Y (en) * | 2018-05-03 | 2018-09-13 | Alcaide Francisco Vergara | PIPETTE FOR THE SUPPORT OF OVOCITS FOR PROCEDURES OF SPRAY INJECTION WITHOUT CYTOPLASMATIC ASPIRATION |
| CN109929759B (en) * | 2019-03-22 | 2021-07-20 | 山东农业大学 | Collection and early-stage forming culture device for oocyte |
| CN109852533B (en) * | 2019-04-02 | 2022-02-25 | 泰山医学院 | High-efficiency oocyte separation and collection device for animal ovary |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0521674A1 (en) * | 1991-07-01 | 1993-01-07 | Tanja Dominko | In vitro maturation of denuded bovine oocytes |
| CN2722861Y (en) * | 2004-07-07 | 2005-09-07 | 刘璋 | Micro operation egg holder |
| CN200980715Y (en) * | 2006-08-17 | 2007-11-28 | 吕祁峰 | Novel microscopic ovum holding tube |
| CN101797189A (en) * | 2010-02-23 | 2010-08-11 | 湖北省农业科学院畜牧兽医研究所 | Apparatus for collecting early-stage embryo or early-stage oocyte of pig and collecting method |
| CN103289891A (en) * | 2013-04-23 | 2013-09-11 | 青海省畜牧兽医科学院 | Fast bovine oocyte collector |
-
2016
- 2016-05-11 CN CN201610313218.1A patent/CN105838581B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0521674A1 (en) * | 1991-07-01 | 1993-01-07 | Tanja Dominko | In vitro maturation of denuded bovine oocytes |
| CN2722861Y (en) * | 2004-07-07 | 2005-09-07 | 刘璋 | Micro operation egg holder |
| CN200980715Y (en) * | 2006-08-17 | 2007-11-28 | 吕祁峰 | Novel microscopic ovum holding tube |
| CN101797189A (en) * | 2010-02-23 | 2010-08-11 | 湖北省农业科学院畜牧兽医研究所 | Apparatus for collecting early-stage embryo or early-stage oocyte of pig and collecting method |
| CN103289891A (en) * | 2013-04-23 | 2013-09-11 | 青海省畜牧兽医科学院 | Fast bovine oocyte collector |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105838581A (en) | 2016-08-10 |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171110 Termination date: 20180511 |
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| CF01 | Termination of patent right due to non-payment of annual fee |