CN204888278U - Plant stem apex stripping off device - Google Patents
Plant stem apex stripping off device Download PDFInfo
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- CN204888278U CN204888278U CN201520456079.9U CN201520456079U CN204888278U CN 204888278 U CN204888278 U CN 204888278U CN 201520456079 U CN201520456079 U CN 201520456079U CN 204888278 U CN204888278 U CN 204888278U
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- pin cutter
- stem apex
- plant stem
- connecting portion
- stripping
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Abstract
The utility model provides a plant stem apex stripping off device, include: needle sword, handle, needle sword hold in the palm, it is connected to adopt the needle sword to hold in the palm between handle and the needle sword, needle sword front end forms the scarf through the processing of cutting sth. Askew. The utility model provides a plant stem apex stripping off device is similar to medical syringe structure, and its key feature is quality light (about 5g), and easy taking is held, and important function unit position (needle sword) is small, easily observes the operation under the anatomical lens high power, and the firing disinfection is rapid, and cool time is short.
Description
Technical field
The utility model relates to plant cultivating device technical field, particularly relates to a kind of plant stem apex stripping off device.
Background technology
Potatoes cultivated area has more than 570 ten thousand hm
2, occupy first place in the world, but the lower (15.45thm-of yield level
2), not only lower than the average per unit area yield (17.26thm-in the world
2), more not as good as World Developed Countries (50.25thm-
2).Virus disease harm is one of principal element affecting potatoes output, because potato is vegetative reproduction, virus accumulates year by year with potato breeding, cause potato seed sexual involution, research proves that Potyvirus causes the potato underproduction 30% ~ 50%, if different virus mixed infection, then can cause the production loss of more than 80%.It is reported, the virus of potato-infecting reaches more than 30 kinds, Chinese hazard ratio more serious mainly contain potato virus X (PVX) (underproduction 10-20%), marmor upsilon (PVY) (underproduction 50-80%), potato virus S (PVS) (underproduction 10-20%), marmor solani (PVA) (underproduction more than 40%), marmor angliae (PVM) (weak strain underproduction 10-20%, strong strain underproduction 60-70%) and corium solani (PLRV) (underproduction 40-70%).
Carrying out Virusfree by stem-apex Meristem culture is poison-removing method the earliest, and the method obtains the generally accreditation of researcher, uses till today always.The cardinal principle of stem apex detoxify there is competitive relation between cell division and virus replication, in active shoot apical meristem, normal nucleoprotein synthesis is preponderated, virion can not get the nutrition that copies and is suppressed, therefore the distribution of virus in plant body is uneven, and wherein especially growing point (about 0.1 ~ 1.0mm region) band virus is minimum or be not with virus for stem apex part, and the closer to tip, virus concentration is lower, infects probability also lower.Therefore, researchers have successfully turned out virus-elimination seedlings and potato seed according to this principle, effectively solve potato degeneration problem.
The operation tool applying stem apex stripping is needed mainly to apply tweezers collocation scalpel and dissecting needle at present, tweezers mainly fix overburden in the visual field of anatomical lens, then peel off with dissecting needle or scalpel the leaf wrapped up outside stem apex, finally expose the stem apex of suitable size, cut in the corresponding medium of access with scalpel and cultivate.
And the operation tool that current existing stem apex is peeled off exists following shortcoming: 1, scalpel heavy (20g), operating time long fatiguability, affects operating efficiency; 2, scalpel knife face is comparatively large, and under high power anatomical lens, (40 ×) are concerning the crop that will peel off stem apex less (0.1-0.3mm), impact operation; 3, scalpel knife face is comparatively large, and calcination sterilization elapsed time is long, and cooling is slow, affects operating efficiency; 4, scalpel knife face is comparatively large, and sterilization is not thorough, easily causes external source to pollute, and easily causes cross-infection (easily encountering band diseased tissues) in operating process, affects stem apex quality; 5, the most advanced and sophisticated function without cutter of dissecting needle, the stem apex stripped cannot be cut, and needs by cutter class instrument, increases operation sequence, affects operating efficiency; 6, dissecting needle, handle is meticulous, not easily by holding, affects operating efficiency; 7, dissecting needle, diameter is comparatively thick, and calcination is sterilized length consuming time, affects operating efficiency.
Utility model content
The purpose of this utility model is the defect solving the existence of above-mentioned prior art, the plant stem apex stripping off device providing a kind of portable construction and namely can shell, also can cut.
A kind of plant stem apex stripping off device, comprising: pin cutter, handle, the holder of pin cutter, adopts the holder of pin cutter to be connected between described handle with pin cutter, and described pin cutter front end, through chamfering process, forms scarf.
Further, plant stem apex stripping off device as above, described handle comprises handle body, pin cutter holder connecting portion, and described handle body is the cylindrical type long tube of hollow, described pin cutter holder connecting portion is tapered tubular structure, and its external diameter is progressively transitioned into 4mm by 5mm.
Further, plant stem apex stripping off device as above, between handle body pin cutter holder connecting portion, be provided with a groove width is the draw-in groove of 3mm, and the snap ring thickness of this draw-in groove is 0.8mm.
Further, plant stem apex stripping off device as above, the holder of described pin cutter is pyramidal structure, and comprise connecting portion and pin cutter installation portion two parts, described connecting portion is length 10mm hollow taper structure, wall thickness 1mm, and internal diameter is transitioned into 4mm gradually by 5mm; Described pin cutter connecting portion is the hollow tubular structures of length 15mm, and the hollow pipe inner diameter at its center is 1mm, and this hollow pipe is convenient to pin cutter to be combined with it solidly.
Further, plant stem apex stripping off device as above, is fixed with a stop part in the hollow taper inside configuration of connecting portion, and this stop part continues to move for stoping pin cutter backward, and the thickness of this stop part is 1mm.
Further, plant stem apex stripping off device as above, described pin cutter adopts stainless steel to make.
Further, plant stem apex stripping off device as above, described handle and the holder of pin cutter all adopt polypropylene, polystyrene, styrene/acrylonitrile copolymer one wherein to make.
The utility model relates to the plant stem apex stripping off device provided, be similar to injector for medical purpose structure, its main feature is quality light (about 5g), be easy to by holding, critical function position (pin cutter) volume is little, is easy to observe operation under anatomical lens high power, calcination sterilization rapidly, cool time is short, and entirety can autoclaving, recycles.
Accompanying drawing explanation
Fig. 1 is the utility model plant stem apex stripping off device structural representation;
Fig. 2 is the utility model handle holder structure schematic diagram;
Fig. 3 is the utility model pin cutter holder structure schematic diagram;
Fig. 4 is the utility model pin knife structure schematic diagram.
Embodiment
For making the purpose of this utility model, technical scheme and advantage clearly, below technical scheme in the utility model be clearly and completely described, obviously, described embodiment is the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
As shown in Figure 1, the plant stem apex stripping off device that the utility model provides, comprises pin cutter 3, handle 1, pin cutter holder 2, and described handle 1 is connected with adopting pin cutter holder 2 between pin cutter 3.Total length is 179.8mm, is convenient to stem apex to be inoculated in being equipped with in the test tube of the dark stem apex special culture media of 40mm of length 150mm.Handle and the holder of pin cutter adopt polypropylene, polystyrene, styrene/acrylonitrile copolymer one wherein, the advantages such as material has high temperature resistant, corrosion-resistant, and material is light, can autoclaving, recycle.
Described handle 1 and pin cutter holder 2 all adopt polypropylene, polystyrene, styrene/acrylonitrile copolymer one wherein to make, the advantages such as material has high temperature resistant, corrosion-resistant, and material is light.
As shown in Figure 2, described handle 1 overall length 123.8mm, described handle comprises handle body 11, pin cutter holder connecting portion 12.Described handle body 11 is the cylindrical type long tube of hollow, and length 120mm, wall thickness 0.8mm, internal diameter 10mm, be convenient to hand-held.Described pin cutter holder connecting portion 12 is the tubular structure of length 10mm, and its external diameter is progressively transitioned into 4mm (being 5mm near one end external diameter of handle) by 5mm.The draw-in groove 13 that a groove width is 3mm is provided with between handle body 11, pin cutter holder connecting portion 12, snap ring 131 thickness of this draw-in groove 13 is 0.8mm, described draw-in groove 13 is convenient on the culture dish sidewall of pin cutter fixed placement under anatomical lens (thickness 2mm) in stem apex stripping process, make both hands can do other things, stopped pin cutter simultaneously and left about the pollution caused.
As shown in Figure 3, described pin cutter holder 2 is pyramidal structure, comprises connecting portion 22 and pin cutter installation portion 23 two parts, total length 26mm.Described connecting portion 22 is length 10mm hollow taper structure, wall thickness 1mm, internal diameter is transitioned into 4mm (one end diameter near pin cutter installation portion is 4mm) gradually by 5mm, and this hollow taper structure is convenient to pin cutter holder connecting portion 12 to be inserted therein portion and strong bonded; Described pin cutter connecting portion 23 is the hollow tubular structures of length 15mm, and hollow pipe 231 inner diameter at its center is 1mm, and this hollow pipe 231 is convenient to pin cutter to be combined with it solidly.
Further, a stop part 21 is fixed with in the hollow taper inside configuration of connecting portion 22, this stop part 21 continues to move for stoping pin cutter backward, the thickness of described stop part 21 is 1mm, if stop part 21 is too thick, handle and the holder of pin cutter can in conjunction with not tight, and therefore, thickness is limited to 1mm by the utility model.
As shown in Figure 4, described pin cutter 3 adopts the ultra-thin stainless steel of resistance to calcining, and pin cutter front end is through chamfering process, form scarf 31, pin point of a knife end possesses the function of pin, and pin point of a knife end scarf 31 possesses the function of cutter, meets in stem apex stripping process the action need shelled and cut simultaneously.
Described pin cutter 3 adopts resistance to calcining 304 or 316 ultra-thin stainless steel capillary material, external diameter 0.9mm, internal diameter 0.3mm, wall thickness 0.3mm, total length 45mm, clear length 30mm after being combined with the holder of pin cutter, be convenient to the calcination of alcolhol burner flame, in order to avoid calcination is to the holder of pin cutter, pin cutter front end is through chamfering process, form tangent plane, tip top possesses the function of pin, cut sth. askew and possess the function of cutter in tip, meet in stem apex stripping process the action need shelled and cut simultaneously, pin cutter is thinner, alcolhol burner calcination 2 can reach the object of sterilization second, and cooling is very fast, do not affect use, pin cutter and the holder of pin cutter are 15mm in conjunction with length, can guarantee to combine firmly, convenient operation, outside clear length is 55mm, be a bit larger tham the radius length (45mm) of culture dish, be convenient to strip operation, hand is by chance placed in outside culture dish, decreasing pollution.The holder of pin cutter and handle complement one another, pin cutter and the holder of pin cutter replaceable at any time, be applicable to shell in enormous quantities stem apex and use.Meanwhile, two pin cuttves can operate under the microscope, one for fixing pending tissue, another, for peeling off and cutting stem apex position, because pin point of a knife end is tiny, disturbs little in field of microscope, convenient and practical, effective.
The utility model relates to the plant stem apex stripping off device provided, be similar to injector for medical purpose structure, its main feature is quality light (about 5g), be easy to by holding, critical function position (pin cutter) volume is little, be easy to observe operation under anatomical lens high power, rapidly, cool time is short in calcination sterilization.
Potato Shoot-tips stripping off device operating process:
Step 1: the number of the Potato Cultivars (being) processed as required, prepares complete stem apex exfoliation tool, at least 2 covering devices/kind (being);
Step 2: wrap stem apex stripping off device (handle and pin cutter are separately) with newspaper, put into high-pressure sterilizing pot sterilizing 20 minutes, cool for subsequent use;
Step 3: under the aseptic condition of superclean bench, opens stem apex stripping off device and assembles, and with alcolhol burner flame calcination pin cutter, cools for subsequent use;
Step 4: both hands respectively hold set of device, stem apex stripping is carried out in the field of microscope of certain multiplication factor, set of device is used for immobilization material, another set of device is used for stem apex and peels off, pin point of a knife end needle point position is utilized to peel off the siphonal lobe of parcel shoot tip meristem, until only surplus growing point and a leaf primordium in the visual field, then the pin cutter calcination sterilization will being used for peeling off, the sharp knife face position of pin point of a knife end side surface is utilized to cut growing point tissue with a leaf primordium after cooling, be placed in rapidly the test tube filling stem apex special culture media, seal test tube mouth, carry out mark.
Step 5: the pin cutter utilizing alcolhol burner flame calcination two covering device again, carries out next round operation after cooling.
Last it is noted that above embodiment is only in order to illustrate the technical solution of the utility model, be not intended to limit; Although be described in detail the utility model with reference to previous embodiment, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of each embodiment technical scheme of the utility model.
Claims (7)
1. a plant stem apex stripping off device, it is characterized in that, comprise: pin cutter (3), handle (1), pin cutter holder (2), pin cutter holder (2) is adopted to be connected between described handle (1) with pin cutter (3), described pin cutter (3) front end, through chamfering process, forms scarf (31).
2. plant stem apex stripping off device according to claim 1, it is characterized in that, described handle (1) comprises handle body (11), pin cutter holder connecting portion (12), the cylindrical type long tube that described handle body (11) is hollow, described pin cutter holder connecting portion (12) is tapered tubular structure, and its external diameter is progressively transitioned into 4mm by 5mm.
3. plant stem apex stripping off device according to claim 2, it is characterized in that, between handle body (11) pin cutter holder connecting portion (12), be provided with the draw-in groove (13) that a groove width is 3mm, snap ring (131) thickness of this draw-in groove (13) is 0.8mm.
4. plant stem apex stripping off device according to claim 1, it is characterized in that, described pin cutter holder (2) is pyramidal structure, comprise connecting portion (22) and pin cutter installation portion (23) two parts, described connecting portion (22) is length 10mm hollow taper structure, wall thickness 1mm, internal diameter is transitioned into 4mm gradually by 5mm; The hollow tubular structures that described pin cutter connecting portion (23) is length 15mm, hollow pipe (231) inner diameter at its center is 1mm, and this hollow pipe (231) is convenient to pin cutter to be combined with it solidly.
5. plant stem apex stripping off device according to claim 4, it is characterized in that, a stop part (21) is fixed with in the hollow taper inside configuration of connecting portion (22), this stop part (21) continues to move for stoping pin cutter backward, and the thickness of this stop part (21) is 1mm.
6. plant stem apex stripping off device according to claim 1, is characterized in that, described pin cutter (3) adopts stainless steel to make.
7. plant stem apex stripping off device according to claim 1, is characterized in that, described handle (1) and pin cutter holder (2) all adopt polypropylene, polystyrene, styrene/acrylonitrile copolymer one wherein to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520456079.9U CN204888278U (en) | 2015-06-29 | 2015-06-29 | Plant stem apex stripping off device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520456079.9U CN204888278U (en) | 2015-06-29 | 2015-06-29 | Plant stem apex stripping off device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204888278U true CN204888278U (en) | 2015-12-23 |
Family
ID=54910518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520456079.9U Expired - Fee Related CN204888278U (en) | 2015-06-29 | 2015-06-29 | Plant stem apex stripping off device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204888278U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150145A (en) * | 2018-03-30 | 2019-08-23 | 广安职业技术学院 | A kind of tissue culture experiment cutter being convenient for changing cutter head |
| CN110547201A (en) * | 2019-09-30 | 2019-12-10 | 安徽省农业科学院棉花研究所 | Plant stem tip separator that can measure |
| CN110547201B (en) * | 2019-09-30 | 2024-05-31 | 安徽省农业科学院棉花研究所 | Measurable plant stem tip separating device |
-
2015
- 2015-06-29 CN CN201520456079.9U patent/CN204888278U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150145A (en) * | 2018-03-30 | 2019-08-23 | 广安职业技术学院 | A kind of tissue culture experiment cutter being convenient for changing cutter head |
| CN110547201A (en) * | 2019-09-30 | 2019-12-10 | 安徽省农业科学院棉花研究所 | Plant stem tip separator that can measure |
| CN110547201B (en) * | 2019-09-30 | 2024-05-31 | 安徽省农业科学院棉花研究所 | Measurable plant stem tip separating device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151223 Termination date: 20180629 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |