CN105838581A - Animal ovary oocyte separation and collection device - Google Patents
Animal ovary oocyte separation and collection device Download PDFInfo
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- CN105838581A CN105838581A CN201610313218.1A CN201610313218A CN105838581A CN 105838581 A CN105838581 A CN 105838581A CN 201610313218 A CN201610313218 A CN 201610313218A CN 105838581 A CN105838581 A CN 105838581A
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- oocyte
- egg apparatus
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- ovary
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- Bioinformatics & Cheminformatics (AREA)
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- Biomedical Technology (AREA)
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Abstract
The invention discloses an animal ovary oocyte separation and collection device. The device comprises an oocyte holder, a supporting disc and a collection vessel. An oocyte holder pushing rod is used for up and down pushing in the oocyte holder. An oocyte holder chuck is arranged at the bottom of the oocyte holder. The oocyte holder chuck and the oocyte holder pushing rod are connected into a whole. The supporting disc is arranged below the oocyte holder. The supporting disc is in the shape of an inwards-concaved circular ring and is inwards concaved to form a containing port. The containing port is connected with an adjustable knife-holding ring in a suspended mode through a connecting piece. Cutting knives are uniformly arranged on the adjustable knife-holding ring. The collection vessel is arranged below the supporting disc and used for collecting oocytes from an ovary. The device has the advantages that time and labor are saved when the oocytes are collected, efficiency is high, cost is low, and use is easy and convenient.
Description
Technical field
The present invention relates to Cell collection device equipment, particularly a kind of animal ovary oocyte separates and collects dress
Put.
Background technology
The oocyte of animal is also called ovum, and ovum is that a new living individual occurs with the combination of sperm
The premise grown and basis.Along with the development of modern life science, oocyte is rearing new variety, turns base
Important materials because of the aspect such as breeding and breed improvement.The acquisition of animal oocyte usually derives from and butchers
The animal ovary tissue that field provides, after animal slaughtering, animal ovary tissue can not eat, and separates if it goes without doing
Collecting oocyte, often can go out of use process.Can isolate big in the in vitro animal ovary tissue of slaughterhouse
The oocyte of amount is for related works such as rearing new variety, transgenic breeding and breed improvements.
At present, the method separating oocyte conventional in the in vitro animal ovary tissue of slaughterhouse has: 1 suction method,
Suction method is exactly to use syringe needle to thrust gently in the follicle of ovary tissue to draw oocyte and follicle
Liquid, after often inhaling 2～5 follicles, slowly injects the liquid in syringe in ovoscopy cup, in case checking.2
Patterning method, cuts ovary in length and breadth with hand-held razor blade, the liquid of recovery is placed on static in ovoscopy cup after,
Putting ovoscopy cup and pick oocyte under microscope, this program is time-consuming.3 stripping methods, with Sterile ophthalmic shears or
Blade, around follicle, separates it with surrounding tissue, takes off complete circular follicle, is put into added with training
In the plate of nutrient solution, cutting follicle, follicular fluid flows out and with oocyte from follicle, and this program is complicated
With inefficient.When gathering Oocytes, the ovary substantial amounts sent here due to slaughterhouse is with in vitro
In ovary, oocyte must gather out to improve the quality of oocyte as early as possible, so improving collecting efficiency
It is to solve oocyte to separate and collect the key of process with acquisition quality.At present, the most also do not have
Being specifically designed to animal ovary oocyte and separate and collect device, according to production practices, we design and have invented one
Planting oocyte in the in vitro ovary of animal and separate and collect device, it is time saving and energy saving that this device has, and high efficiency is low
Cost, the advantage such as easy to use.
Summary of the invention
It is an object of the invention to provide a kind of animal ovary oocyte and separate and collect device.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of animal ovary oocyte separates and collects device, including holding egg apparatus, a support plate and collecting three, ware
Part, described in hold and push up and down by holding egg apparatus push rod inside egg apparatus, described in hold and be provided with bottom egg apparatus
Hold egg apparatus chuck, described hold egg apparatus chuck and hold egg apparatus push rod and be connected as a single entity, described in hold below egg apparatus and be
Support plate, described support plate is the toroidal caved inward, and mouth is placed in its formation that caves inward, described in put
Put mouth by the adjustable cutter ring of holding of the unsettled connection of connector one, described adjustable hold to be evenly arranged on cutter ring cut
Cutter, described collection ware is arranged on below a support plate collects the oocyte deriving from ovary.
Described connector is retractable material, and described adjustable cutter ring of holding relies on connector telescopic adjustment size.
Described cutter have multiple.
Beneficial effects of the present invention: it is time saving and energy saving that the present invention has oocyte collection, high efficiency, low cost,
The advantage such as easy to use.
Accompanying drawing explanation
Fig. 1 is present configuration schematic diagram.
Fig. 2 is support plate structure schematic diagram of the present invention.
Labelling in figure, 1-holds egg apparatus push rod, and 2-holds egg apparatus, and 3-holds egg apparatus chuck, and 4-props up support plate, and 5-collects
Ware, 6-places mouth, the adjustable cutter ring of holding of 7-, 8-cutter, 9-connector.
Detailed description of the invention
With specific embodiment, the present invention is described in further detail below in conjunction with the accompanying drawings.
As it is shown in figure 1, it is an object of the invention to provide a kind of animal ovary oocyte to separate and collect device,
Including holding egg apparatus 2, support plate 4 and collecting 5 three parts of ware, described in hold that egg apparatus 2 is internal to be pushed away by holding egg apparatus
Send bar about 1 to push, described in hold to be provided with bottom egg apparatus 2 and hold egg apparatus chuck 3, described holds egg apparatus chuck
3 and hold egg apparatus push rod 1 and be connected as a single entity, described in hold below egg apparatus 2 as a support plate 4, described support plate 4 is
The toroidal caved inward, its formation placement mouth 6 that caves inward, described placement mouth 6 is hanged by connector 9
One adjustable cutter ring 7 of holding of empty connection, described adjustable holding is evenly arranged cutter 8 on cutter ring 4, described receipts
Collection ware 5 is arranged on below support plate 4 collects the oocyte deriving from ovary, and described connector 9 is for stretching
Compression material, described adjustable cutter ring 7 of holding relies on connector 9 telescopic adjustment size, described cutter 8 to have
Multiple.
During use, a support plate 4 being placed on oocytes collection ware 5, hands grips egg apparatus 2, with holding egg apparatus 2
Chuck ovary that ovary is put into support plate 4 place mouth 6, pressing push rod 1 makes ovary hold through adjustable
Cutter ring 7, utilizes cutter 8 to complete ovary cutting, after ovary cutting, gets collection ware 5 and picks under the microscope
Oocyte.
Above content is only to present configuration example and explanation, the technology of affiliated the art
Described specific embodiment is made various amendment or supplements or use similar mode to substitute by personnel,
Without departing from invention structure or surmount scope defined in the claims, the present invention all should be belonged to
Protection domain.
Claims (3)
1. animal ovary oocyte separates and collects a device, including hold egg apparatus (2), support plate (4) and
Collect (5) three parts of ware, it is characterised in that described in hold egg apparatus (2) internal by holding egg apparatus push rod
(1) push up and down, described in hold egg apparatus (2) bottom and be provided with and hold egg apparatus chuck (3), described holds ovum
Device chuck (3) and hold egg apparatus push rod (1) and be connected as a single entity, described in hold egg apparatus (2) lower section for a support plate (4),
Described support plate (4) is the toroidal caved inward, and mouth (6), described placement are placed in its formation that caves inward
Mouthful (6), by the adjustable cutter ring (7) of holding of connector (9) unsettled connection one, described adjustable hold cutter ring
(7) being evenly arranged cutter (8) on, described collection ware (5) is arranged on support plate (4) lower section and collects source
Oocyte in ovary.
The most according to claim 1, a kind of animal ovary oocyte separates and collects device, it is characterised in that
Described connector (9) is retractable material, and described adjustable cutter ring (7) of holding relies on connector (9) to stretch
Adjusting size.
The most according to claim 1, a kind of animal ovary oocyte separates and collects device, it is characterised in that
Described cutter (8) have multiple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610313218.1A CN105838581B (en) | 2016-05-11 | 2016-05-11 | A kind of animal ovary egg mother cell separates and collects device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610313218.1A CN105838581B (en) | 2016-05-11 | 2016-05-11 | A kind of animal ovary egg mother cell separates and collects device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105838581A true CN105838581A (en) | 2016-08-10 |
| CN105838581B CN105838581B (en) | 2017-11-10 |
Family
ID=56591944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610313218.1A Expired - Fee Related CN105838581B (en) | 2016-05-11 | 2016-05-11 | A kind of animal ovary egg mother cell separates and collects device |
Country Status (1)
| Country | Link |
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| CN (1) | CN105838581B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109852533A (en) * | 2019-04-02 | 2019-06-07 | 泰山医学院 | A kind of high efficiency egg mother cell of animal ovary separates and collects device |
| CN109929759A (en) * | 2019-03-22 | 2019-06-25 | 山东农业大学 | A kind of acquisition of egg mother cell and early stage molding culture apparatus |
| CN112367936A (en) * | 2018-05-03 | 2021-02-12 | 弗朗西斯科·贝尔加拉·阿尔凯德 | Oocyte retention pipette for use in a sperm injection method without cytoplasmic aspiration |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0521674A1 (en) * | 1991-07-01 | 1993-01-07 | Tanja Dominko | In vitro maturation of denuded bovine oocytes |
| CN2722861Y (en) * | 2004-07-07 | 2005-09-07 | 刘璋 | Micro operation egg holder |
| CN200980715Y (en) * | 2006-08-17 | 2007-11-28 | 吕祁峰 | Novel microscopic ovum holding tube |
| CN101797189A (en) * | 2010-02-23 | 2010-08-11 | 湖北省农业科学院畜牧兽医研究所 | Apparatus for collecting early-stage embryo or early-stage oocyte of pig and collecting method |
| CN103289891A (en) * | 2013-04-23 | 2013-09-11 | 青海省畜牧兽医科学院 | Fast bovine oocyte collector |
-
2016
- 2016-05-11 CN CN201610313218.1A patent/CN105838581B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0521674A1 (en) * | 1991-07-01 | 1993-01-07 | Tanja Dominko | In vitro maturation of denuded bovine oocytes |
| CN2722861Y (en) * | 2004-07-07 | 2005-09-07 | 刘璋 | Micro operation egg holder |
| CN200980715Y (en) * | 2006-08-17 | 2007-11-28 | 吕祁峰 | Novel microscopic ovum holding tube |
| CN101797189A (en) * | 2010-02-23 | 2010-08-11 | 湖北省农业科学院畜牧兽医研究所 | Apparatus for collecting early-stage embryo or early-stage oocyte of pig and collecting method |
| CN103289891A (en) * | 2013-04-23 | 2013-09-11 | 青海省畜牧兽医科学院 | Fast bovine oocyte collector |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112367936A (en) * | 2018-05-03 | 2021-02-12 | 弗朗西斯科·贝尔加拉·阿尔凯德 | Oocyte retention pipette for use in a sperm injection method without cytoplasmic aspiration |
| CN109929759A (en) * | 2019-03-22 | 2019-06-25 | 山东农业大学 | A kind of acquisition of egg mother cell and early stage molding culture apparatus |
| CN109852533A (en) * | 2019-04-02 | 2019-06-07 | 泰山医学院 | A kind of high efficiency egg mother cell of animal ovary separates and collects device |
| CN109852533B (en) * | 2019-04-02 | 2022-02-25 | 泰山医学院 | High-efficiency oocyte separation and collection device for animal ovary |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105838581B (en) | 2017-11-10 |
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