CN109852533B - High-efficiency oocyte separation and collection device for animal ovary - Google Patents
High-efficiency oocyte separation and collection device for animal ovary Download PDFInfo
- Publication number
- CN109852533B CN109852533B CN201910260586.8A CN201910260586A CN109852533B CN 109852533 B CN109852533 B CN 109852533B CN 201910260586 A CN201910260586 A CN 201910260586A CN 109852533 B CN109852533 B CN 109852533B
- Authority
- CN
- China
- Prior art keywords
- bottom plate
- welded
- plate
- rod
- limiting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000287 oocyte Anatomy 0.000 title claims abstract description 28
- 241001465754 Metazoa Species 0.000 title claims abstract description 24
- 210000001672 ovary Anatomy 0.000 title claims abstract description 24
- 238000000926 separation method Methods 0.000 title claims abstract description 17
- 238000005520 cutting process Methods 0.000 claims abstract description 36
- 238000003825 pressing Methods 0.000 claims abstract description 29
- 230000007246 mechanism Effects 0.000 claims abstract description 27
- 239000000945 filler Substances 0.000 claims description 25
- 238000004140 cleaning Methods 0.000 claims description 22
- 238000007789 sealing Methods 0.000 claims description 18
- 239000004744 fabric Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000003860 storage Methods 0.000 claims description 10
- 230000000149 penetrating effect Effects 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 2
- 230000009471 action Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 239000000428 dust Substances 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 238000003466 welding Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a high-efficiency oocyte separation and collection device for an animal ovary, which comprises a bottom plate, wherein a feeding chute is formed in the middle of the top end of the bottom plate, a guide shaft is connected to the bottom of the feeding chute in a rotating mode at equal intervals, a rubber sleeve is sleeved in the middle of the guide shaft, a limiting plate is welded at the top end of the bottom plate at equal intervals, a separation mechanism is arranged at the position, corresponding to the limiting plate, of the top end of the bottom plate, the device is scientific and reasonable in structure and safe and convenient to use, a culture dish is placed in the feeding chute in advance, the purpose of cutting is achieved by pressing a lower pressing plate through a connecting ring and a cutting blade, the original position is restored through a reset spring, the culture dish is pushed conveniently and continuously cut through the action of the rubber sleeve and the guide shaft, the purposes of quick operation and convenient cutting are achieved, and high-efficiency oocyte separation is achieved, meanwhile, the limiting plate is used for avoiding the contact between the cutting blade and the bottom end of the culture dish, and the damage to the cutting blade is avoided.
Description
Technical Field
The invention relates to the technical field of cell collection devices, in particular to a high-efficiency oocyte separation and collection device for an animal ovary.
Background
The combination of ova and sperms is a premise and a foundation for development of a new life individual, a certain amount of oocytes are contained in an animal ovary, the oocytes are taken out from the slaughtered animal ovary, and then scientific research work in various aspects such as new variety cultivation, transgenic breeding, variety improvement and the like is carried out, the conventional oocyte separation method comprises a suction method, a cutting method and a stripping method, the conventional oocyte separation method adopts more precise equipment, the operation steps are complicated in the separation process, a large number of animal ovaries are contained in a slaughter house, the complicated operation easily causes the separation efficiency to be reduced, further partial oocytes lose activity, the waste of raw materials is caused, meanwhile, the conventional device can not be cleaned in the use process, the surface of a cutting blade is easily infected with mucus, and the cutting effect is reduced, therefore, there is a high necessity for a device for separating and collecting oocytes from animal ovaries, which is highly efficient.
Disclosure of Invention
The invention provides a high-efficiency oocyte separating and collecting device for an animal ovary, which can effectively solve the problems that equipment adopted in a common oocyte separating mode in the background technology is fine, operation steps are complex in the separating process, a large number of animal ovaries are contained in a slaughterhouse, the separating efficiency is easy to reduce due to complex operation, partial oocytes lose activity, raw material waste is caused, and meanwhile, the conventional device cannot be cleaned frequently in the using process, mucus is easily adhered to the surface of a cutting blade, and the cutting effect is reduced.
In order to achieve the purpose, the invention provides the following technical scheme: the high-efficiency oocyte separating and collecting device for the animal ovary comprises a bottom plate, wherein a feeding chute is formed in the middle of the top end of the bottom plate, guide shafts are rotatably connected to the bottom of the feeding chute at equal intervals, and rubber sleeves are sleeved in the middle of the guide shafts;
limiting plates are welded at the top end of the bottom plate at equal intervals, the number of the limiting plates is three, two limiting plates are respectively positioned at two sides of the feeding chute, corner baffles are welded at positions, corresponding to the limiting plates, of one side of the feeding chute, of the top end of the bottom plate, and water storage boxes are installed between the two corner baffles and the two limiting plates;
the separation mechanism is arranged at the position, corresponding to the limiting plate, of the top end of the bottom plate and comprises an upright post, a lower pressing plate, a return spring, a limiting block, a cleaning chute, a first positioning cushion block, a connecting post, a push plate, a mounting plate, a connecting rod, a connecting ring and a cutting blade;
the bottom plate is characterized in that stand columns are welded at positions, corresponding to the position of the limiting plate, of the edge portion of the top end of the bottom plate, limiting blocks are welded at the top ends of the two stand columns, lower pressing plates are connected to the tops of the two stand columns in a sliding mode, return springs are installed at positions, corresponding to the positions of the stand columns, of the bottom ends of the lower pressing plates, cleaning chutes are formed in the middle of the top ends of the lower pressing plates in a penetrating mode, first positioning cushion blocks are glued at two edges, corresponding to the cleaning chutes, of the two end portions of the top end of the lower pressing plates, connecting columns are connected to the inner ends of the cleaning chutes in a sliding mode, push plates are welded at the top ends of the connecting columns, mounting plates are welded at the bottom ends of the connecting columns, connecting rods are welded at the bottom ends of the connecting rods in a penetrating mode in a centrosymmetric mode, connecting rings are installed at the bottom ends of the connecting rings, and cutting blades are installed at equal intervals at the bottom of the connecting rings;
the inside sliding connection of pay-off spout has the culture dish, the equal symmetrical cementing in culture dish both sides surface has the second location cushion.
Preferably, the distance between the two second positioning cushion blocks positioned on the same side of the culture dish is equal to the width of the limiting plate, the culture dish is square, and the side length of the culture dish is equal to the width of the feeding chute.
Preferably, the stand is located inside reset spring, reset spring one end and holding down plate fixed connection, the reset spring other end and bottom plate fixed connection.
Preferably, the inside clean water liquid that has held of retaining box, it is adjacent distance between the limiting plate equals with retaining box outside width, turning baffle shape is the L type, turning baffle inboard surface laminates with the retaining box mutually.
Preferably, the outer diameter of the connecting column is equal to the width of the cleaning sliding groove, and the bottom ends of the push plates are symmetrically embedded with balls.
Preferably, the bottom end of the bottom plate is provided with a dustproof mechanism, and the dustproof mechanism comprises a first filler strip, a through groove, a second filler strip, a hook, dustproof elastic cloth and a limiting rod;
a first filler strip is welded at one edge part of the bottom end of the bottom plate, and a through groove is formed in the middle of the first filler strip in a penetrating manner;
the welding of bottom plate bottom another limit portion has the second filler strip, couple has all been welded at second filler strip one side surface both ends, the corresponding logical groove position department of second filler strip opposite side surface installs dustproof elasticity cloth, dustproof elasticity cloth one end is passed and is led to the groove joint and have a stopper rod.
Preferably, the length of the limiting rod is equal to that of the first filler strip.
Preferably, the two corners of one edge part of the top end of the bottom plate are respectively welded with a balancing weight, the two sides of the bottom plate are provided with a carrying mechanism, and the carrying mechanism comprises a fixed shaft, a swinging rod, a connecting shaft, a vertical rod, a handle rod, a fixed column, a sealing gasket and a connecting rope;
a fixed shaft is welded at one end part of the two side surfaces of the bottom plate, a swinging rod is rotatably connected in the middle of the fixed shaft, one end of the swinging rod is rotatably connected with a vertical rod through a connecting shaft, and a handle rod is welded between the two vertical rod end parts in a penetrating manner;
the bottom plate is characterized in that a fixing column is welded to the other end of the two side surfaces of the bottom plate, a sealing gasket is connected to one end face of the fixing column in an adhesive mode, and the fixing column is fixedly connected with one end of the oscillating rod through a connecting rope.
Preferably, the length of the connecting rope is equal to that of the swinging rod, and the sum of the lengths of the swinging rod and the vertical rod is equal to that of the bottom plate.
Preferably, the outer side surface of the sealing gasket is provided with anti-skid lines, and the outer side surface of the sealing gasket, the side surface of the swinging rod and the side surface of the vertical rod are all located in the same plane.
Compared with the prior art, the invention has the beneficial effects that: the invention has scientific and reasonable structure and safe and convenient use:
1. in placing the culture dish in advance to the pay-off spout, through pressing down of holding down plate, utilize go-between and cutting blade to realize the cutting purpose, and resume the normal position through reset spring, through the effect of rubber sleeve and guiding axle, convenient promotion culture dish, can change next culture dish and continue to cut, quick operation has been realized, the purpose of convenient cutting, efficient separation oocyte has been reached, avoid cutting blade and culture dish bottom contact through the limiting plate simultaneously, avoid cutting blade's damage.
2. Remove in clean spout through the spliced pole for when pressing down the clamp plate once more, can effectively stretch into cutting blade in the washing water liquid of retaining box, wash cutting blade, glue the thing and be infected with the cutting blade surface in avoiding the animal ovary, the cutting effect who avoids consequently causing reduces, has set up turning baffle and limiting plate simultaneously, realizes quick installation and the dismantlement of retaining box, and be convenient for change the inside water liquid of retaining box, guarantee the cleaning performance who lasts.
3. The utility model discloses a portable culture dish, including the spliced pole, the spliced pole has set up first location cushion for when the spliced pole slides, carry out position restriction to the push pedal through first location cushion, the decline that can be stable when guaranteeing to wash and cutting, the skew of position can not take place, second location cushion has been set up simultaneously, the culture dish is placed to the pay-off spout, can effectively block spacing post card between two second location cushions, realize the location of culture dish, can more accurately place the assigned position with the culture dish when guaranteeing quick replacement culture dish.
4. The dustproof mechanism is arranged, the limiting rod is pulled, dustproof elastic cloth crosses the upper portion of the lower pressing plate, the two ends of the limiting rod are hung and buckled through the hooks, the dustproof elastic cloth can effectively protect parts above the bottom plate, the condition that the parts are corroded to be damaged or covered by dust to be damaged due to external dust is avoided, meanwhile, the first pad strip and the second pad strip are arranged, the bottom plate is effectively prevented from being direct and placed in plane contact, and the situation that the bottom plate is prevented from being wetted to be damaged due to the fact that plane moisture exists and the installation parts are mounted above the bottom plate is avoided.
5. Having set up and having carried the mechanism, through the pulling handle bar, the swinging arms rotates to the swinging arms receives the restriction of connecting the rope, makes the pole setting put vertically, and the user can effectively carry the device through gripping the pole setting this moment, has set up seal gasket simultaneously, is convenient for increase the static friction force of the seal gasket side surface of pole setting side surface, makes pole setting and swinging arms more stable when accomodating.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
In the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the mounting structure of the corner baffle of the present invention;
FIG. 3 is a schematic view of the separating mechanism of the present invention;
FIG. 4 is a schematic view of the mounting structure of the cutting blade of the present invention;
FIG. 5 is a schematic view of the structure of the culture dish of the present invention;
FIG. 6 is a schematic structural view of the dust-proof mechanism of the present invention;
FIG. 7 is a schematic structural view of the carrying mechanism of the present invention;
reference numbers in the figures: 1. a base plate; 2. a feeding chute; 3. a guide shaft; 4. a rubber sleeve; 5. a limiting plate; 6. a corner baffle; 7. a water storage box; 8. a balancing weight;
9. a separating mechanism; 901. a column; 902. a lower pressing plate; 903. a return spring; 904. a limiting block; 905. cleaning the chute; 906. a first positioning cushion block; 907. connecting columns; 908. pushing the plate; 909. mounting a plate; 910. a connecting rod; 911. a connecting ring; 912. a cutting blade;
10. a dust-proof mechanism; 1001. a first filler strip; 1002. a through groove; 1003. a second bead; 1004. hooking; 1005. dustproof elastic cloth; 1006. a limiting rod;
11. a carrying mechanism; 1101. a fixed shaft; 1102. a swing lever; 1103. a connecting shaft; 1104. erecting a rod; 1105. a handle bar; 1106. fixing a column; 1107. sealing gaskets; 1108. connecting ropes;
12. a culture dish; 13. and a second positioning cushion block.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.
Example (b): as shown in figures 1-7, the invention provides a technical scheme, and the high-efficiency oocyte separation and collection device for the animal ovary comprises a bottom plate 1, wherein a feeding chute 2 is formed in the middle of the top end of the bottom plate 1, guide shafts 3 are rotatably connected to the bottoms of the feeding chute 2 at equal intervals, and rubber sleeves 4 are sleeved in the middle of the guide shafts 3.
The welding of 1 top equidistance of bottom plate has limiting plate 5, limiting plate 5 quantity sets up to three, wherein two limiting plates 5 are located 2 both sides of pay-off spout, 2 one side position punishment of corresponding limiting plate 5 position do not on the 1 top of bottom plate have all welded turning baffle 6, install retaining box 7 between two turning baffles 6 and two limiting plates 5, retaining box 7 is inside to have held clean water liquid, distance between the adjacent limiting plate 5 equals with retaining box 7 outside width, 6 shapes of turning baffle are the L type, 6 inboard surfaces of turning baffle are laminated with retaining box 7 mutually, make retaining box 7 can effectively place between two limiting plates 5 and the turning baffle 6, improve the stability when retaining box 7 uses.
The separating mechanism 9 is installed at the position, corresponding to the position of the limiting plate 5, of the top end of the bottom plate 1, and the separating mechanism 9 comprises an upright column 901, a lower pressing plate 902, a return spring 903, a limiting block 904, a cleaning chute 905, a first positioning cushion block 906, a connecting column 907, a push plate 908, a mounting plate 909, a connecting rod 910, a connecting ring 911 and a cutting blade 912.
The top edge of the bottom plate 1 is welded with upright columns 901 at positions corresponding to the position of the limiting plate 5, the top ends of the two upright columns 901 are welded with limiting blocks 904, the tops of the two upright columns 901 are connected with a lower pressing plate 902 in a sliding manner, the bottom end of the lower pressing plate 902 is provided with a return spring 903 at a position corresponding to the position of the upright column 901, the upright columns 901 are positioned inside the return spring 903, one end of the return spring 903 is fixedly connected with the lower pressing plate 902, the other end of the return spring 903 is fixedly connected with the bottom plate 1, the return spring 903 can effectively restore the position of the lower pressing plate 902, the operation convenience is improved, a cleaning chute 905 is arranged in the middle of the top end of the lower pressing plate 902 in a penetrating manner, two end parts of the top end of the lower pressing plate 902 are respectively glued with a first positioning cushion block 906 at two edges corresponding to the cleaning chute 905, one end inside the cleaning chute 905 is connected with a connecting column 907 in a sliding manner, the top end of the connecting column 907 is welded with a pushing plate 908, the outer diameter of the connecting column 907 is equal to the width of the cleaning chute 905, the connecting column 907 can effectively slide along the cleaning sliding groove 905, the bottom end of the push plate 908 is symmetrically embedded with balls, sliding friction is converted into rolling friction, sliding resistance is reduced, and sliding is convenient and fast.
2 inside sliding connection of pay-off spout has culture dish 12, the equal symmetrical veneer in culture dish 12 both sides surface has second location cushion 13, the distance between two second location cushion 13 that are located 12 homonymies of culture dish equals with limiting plate 5's width, be convenient for go into two second location cushion 13 with limiting plate 5 card before, carry out convenient fast along with the location, culture dish 12 is the square, culture dish 12 side length equals with 2 width of pay-off spout, guarantee that culture dish 12 can effectively be filled in and place in the pay-off spout 2.
A first filler strip 1001 is welded at one edge of the bottom end of the bottom plate 1, and a through groove 1002 is formed in the middle of the first filler strip 1001 in a penetrating mode.
The welding of bottom plate 1 bottom another limit portion has second filler rod 1003, and couple 1004 has all been welded at second filler rod 1003 one side surface both ends, and the corresponding logical groove 1002 position department in second filler rod 1003 opposite side surface installs dustproof elasticity cloth 1005, and dustproof elasticity cloth 1005 one end is passed logical groove 1002 and is glued and be had gag lever post 1006, and gag lever post 1006 length equals with first filler rod 1001 length, avoids gag lever post 1006 to pass from leading to the inslot 1002 to guarantee that gag lever post 1006 both ends homoenergetic is effectively hooked by couple 1004.
The balancing weight 8 is welded at the two corners of one edge of the top end of the bottom plate 1, the carrying mechanisms 11 are installed on two sides of the bottom plate 1, and each carrying mechanism 11 comprises a fixed shaft 1101, a swinging rod 1102, a connecting shaft 1103, a vertical rod 1104, a handle bar 1105, a fixed column 1106, a sealing gasket 1107 and a connecting rope 1108.
A fixed shaft 1101 is welded at one end of the two side surfaces of the bottom plate 1, a swinging rod 1102 is rotatably connected to the middle of the fixed shaft 1101, one end of the swinging rod 1102 is rotatably connected with a vertical rod 1104 through a connecting shaft 1103, and a handle bar 1105 penetrates and is welded between the end parts of the two vertical rods 1104.
The other end of the two side surfaces of the bottom plate 1 is welded with a fixed column 1106, one end surface of the fixed column 1106 is glued with a sealing gasket 1107, the outer side surface of the sealing gasket 1107 is provided with anti-skid grains, the outer side surface of the sealing gasket 1107, one side surface of the swinging rod 1102 and one side surface of the vertical rod 1104 are all positioned in the same plane, the sealing gasket 1107 is attached to the side surface of the upright 1104, the storage stability of the upright 1104 is ensured by a large static friction force, the fixed column 1106 is fixedly connected with one end of the swinging rod 1102 by a connecting rope 1108, the length of the connecting rope 1108 is equal to that of the swinging rod 1102, make pole setting 1104 can effectively put to the bottom plate 1 middle part directly over for it is more stable when lifting up, the length sum of swinging arms 1102 and pole setting 1104 equals with bottom plate 1 length, is convenient for place pole setting 1104 and swinging arms 1102 to bottom plate 1 both sides, makes handle bar 1105 can not disturb the normal work of device.
The working principle and the using process of the invention are as follows: when the high-efficiency oocyte separation and collection device for the animal ovary is used, firstly, the animal ovary sent by a slaughterhouse is placed into a plurality of culture dishes 12, then the culture dishes 12 are placed into a feeding chute 2, the culture dishes 12 are pushed, the rubber sleeve 4 is driven by the culture dishes 12, the guide shaft 3 and the rubber sleeve 4 rotate to enable the culture dishes 12 to move more stably, one culture dish 12 moves to the position of a limiting plate 5 on two sides of the feeding chute 2, the limiting plate 5 is clamped between two second positioning cushion blocks 13 to realize rapid positioning, then, a push plate 908 is pushed, the push plate 908 drives a connecting column 907 to move to one end of a cleaning chute 905 along the cleaning chute 905, the push plate is clamped by a first positioning cushion block 906, the connecting column 907 and parts below the connecting column 908 are fixed, then, the press plate 902 is pressed, the tops of two upright columns 901 are both in sliding connection with a lower press plate 902, the lower pressing plate 902 moves downwards along the upright column 901, the mounting plate 909, the connecting rod 910, the connecting ring 911 and the cutting blade 912 move downwards, the cutting blade 912 cuts the animal ovary in the culture dish 12, the oocyte is separated, the cutting mode is simple to operate, the lower pressing plate 902 is effectively supported by the limiting plate 5 through the limiting plate 5, the cutting blade 912 is prevented from contacting with the bottom end of the culture dish 12, the damage of the cutting blade 912 is reduced, after the cutting is finished, the lower pressing plate 902 is loosened, the lower pressing plate 902 returns to the original position under the action of the return spring 903 and is blocked by the limiting block 904 and cannot be separated from the upright column 901, then the pushing plate 908 is pushed again, the connecting rod 907 moves to the other end of the cleaning chute 905, the pressing plate 902 is pressed again at the moment, the mounting plate 909, the connecting rod 910, the connecting ring 911 and the cutting blade 912 move downwards and extend into the water storage box 7, cleaning in clean water liquid in the water storage box 7 to avoid the situation that the cutting efficiency is reduced due to the fact that the surface of the cutting blade 912 is stained with sticky matters, then pushing the second culture dish 12 into the feeding chute 2, installing the steps, positioning and placing again, and separating oocytes again;
when the device needs to be carried, the handle lever 1105 is pulled to pull the handle lever 1105 to the upper part of the middle part of the bottom plate 1, because the fixed column 1106 is fixedly connected with one end of the swinging rod 1102 through the connecting rope 1108, the length of the connecting rope 1108 is equal to that of the swinging rod 1102, so that the vertical rod 1104 can be effectively placed right above the middle part of the bottom plate 1, a user pulls the handle lever 1105 to be connected with the bottom plate 1 through the fixed shaft 1101 through one end of the swinging rod 1102, the other end of the swinging rod 1102 is connected with the fixed column 1106 through the connecting rope 1108, and after being adjusted through the balancing weight 8, the user can more stably lift the handle lever 1105 from the upper part of the middle part of the bottom plate 1, when the carrying mechanism 11 is carried, because the sum of the lengths of the swinging rod 1102 and the vertical rod 1104 and the swinging rod 1104 is equal to the length of the bottom plate 1, the handle lever 1105 is placed to one side of the bottom plate 1105, and the interference of the handle lever with the normal work of the device is avoided, meanwhile, the outer side surface of the sealing gasket 1107 is provided with anti-skid grains, the outer side surface of the sealing gasket 1107, one side surface of the swinging rod 1102 and one side surface of the vertical rod 1104 are all positioned in the same plane, and the sealing gasket 1107 is attached to the side surface of the vertical rod 1104, so that the storage stability of the vertical rod 1104 is ensured through large static friction force;
when the device is accomodate, pulling gag lever post 1006, make gag lever post 1006 drive dustproof elasticity cloth 1005 pass through logical groove 1002, and drive dustproof elasticity cloth 1005 through gag lever post 1006 and cross holding down plate 902 top, make gag lever post 1006 tip block the couple 1004 of second filler strip 1003 tip, and then fix dustproof elasticity cloth 1005, make dustproof elasticity cloth 1005 can effectively protect the part of bottom plate 1 and bottom plate 1 top, the dust falls the device surface when avoiding idle, the effect of first filler strip and second filler strip 1003 simultaneously, make bottom plate 1 can not be direct with place the plane contact, and then avoid placing the wet condition of damaging of bottom plate 1 and bottom plate 1 top installation component that the plane humidity caused.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A high efficiency oocyte separation collection device of animal ovary, includes bottom plate (1), its characterized in that: a feeding chute (2) is formed in the middle of the top end of the bottom plate (1), guide shafts (3) are rotationally connected to the bottom of the feeding chute (2) at equal intervals, and rubber sleeves (4) are sleeved in the middle of the guide shafts (3);
limiting plates (5) are welded at the top end of the bottom plate (1) at equal intervals, the number of the limiting plates (5) is three, two limiting plates (5) are respectively located on two sides of the feeding chute (2), corner baffles (6) are welded at positions, corresponding to the limiting plates (5), of the top end of the bottom plate (1) corresponding to one side of the feeding chute (2), and water storage boxes (7) are installed between the two corner baffles (6) and the two limiting plates (5);
a separation mechanism (9) is arranged at the position, corresponding to the position of the limiting plate (5), of the top end of the bottom plate (1), and the separation mechanism (9) comprises an upright column (901), a lower pressing plate (902), a return spring (903), a limiting block (904), a cleaning sliding groove (905), a first positioning cushion block (906), a connecting column (907), a pushing plate (908), a mounting plate (909), a connecting rod (910), a connecting ring (911) and a cutting blade (912);
upright columns (901) are welded at positions, corresponding to the limiting plates (5), of the edge portion of the top end of the bottom plate (1), limiting blocks (904) are welded at the top ends of the two upright columns (901), lower pressing plates (902) are connected to the tops of the two upright columns (901) in a sliding mode, reset springs (903) are installed at positions, corresponding to the positions of the upright columns (901), of the bottom ends of the lower pressing plates (902), cleaning sliding grooves (905) are formed in the middle of the top ends of the lower pressing plates (902) in a penetrating mode, first positioning cushion blocks (906) are glued to the two edge portions, corresponding to the cleaning sliding grooves (905), of the two end portions of the top end of the lower pressing plates (902), connecting columns (907) are connected to one end of the inner portions of the cleaning sliding grooves (905) in a sliding mode, push plates (908) are welded to the top ends of the connecting columns (907), mounting plates (909) are welded to the bottom ends of the connecting columns (909) in a penetrating mode in a central symmetry mode, a connecting ring (911) is installed at the bottom end of the connecting rod (910), and cutting blades (912) are installed at the bottom of the connecting ring (911) at equal intervals;
a culture dish (12) is connected to the interior of the feeding chute (2) in a sliding manner, and second positioning cushion blocks (13) are symmetrically glued to the surfaces of the two sides of the culture dish (12);
the dustproof mechanism (10) is installed at the bottom end of the bottom plate (1), and the dustproof mechanism (10) comprises a first filler strip (1001), a through groove (1002), a second filler strip (1003), a hook (1004), dustproof elastic cloth (1005) and a limiting rod (1006);
a first filler strip (1001) is welded at one side of the bottom end of the bottom plate (1), and a through groove (1002) is formed in the middle of the first filler strip (1001) in a penetrating mode;
a second pad strip (1003) is welded to the other edge portion of the bottom end of the bottom plate (1), hooks (1004) are welded to two end portions of the surface of one side of the second pad strip (1003), dustproof elastic cloth (1005) is installed on the surface of the other side of the second pad strip (1003) corresponding to the through groove (1002), and one end of the dustproof elastic cloth (1005) penetrates through the through groove (1002) and is glued to a limiting rod (1006);
the portable type water-saving device is characterized in that balancing weights (8) are welded at two corners of one edge of the top end of the bottom plate (1), carrying mechanisms (11) are mounted on two sides of the bottom plate (1), and each carrying mechanism (11) comprises a fixing shaft (1101), a swinging rod (1102), a connecting shaft (1103), a vertical rod (1104), a handle rod (1105), a fixing column (1106), a sealing gasket (1107) and a connecting rope (1108);
a fixed shaft (1101) is welded at one end part of the two side surfaces of the bottom plate (1), a swinging rod (1102) is rotatably connected to the middle part of the fixed shaft (1101), one end of the swinging rod (1102) is rotatably connected with a vertical rod (1104) through a connecting shaft (1103), and a handle rod (1105) penetrates and is welded between the end parts of the two vertical rods (1104);
the sealing device is characterized in that a fixing column (1106) is welded at the other end of the two side surfaces of the bottom plate (1), a sealing gasket (1107) is glued to one end face of the fixing column (1106), and the fixing column (1106) is fixedly connected with one end of the swinging rod (1102) through a connecting rope (1108).
2. The device for separating and collecting the oocytes of the animal ovaries with high efficiency as claimed in claim 1, wherein the distance between the two second positioning cushion blocks (13) on the same side of the culture dish (12) is equal to the width of the limiting plate (5), the culture dish (12) is square, and the side length of the culture dish (12) is equal to the width of the feeding chute (2).
3. The device for separating and collecting the oocytes in the ovaries of the animals as claimed in claim 1, wherein the upright column (901) is positioned inside a return spring (903), one end of the return spring (903) is fixedly connected with the lower pressing plate (902), and the other end of the return spring (903) is fixedly connected with the bottom plate (1).
4. The device for separating and collecting the oocytes of the animal ovaries with high efficiency as claimed in claim 1, wherein the inside of the water storage box (7) is filled with cleaning water, the distance between the adjacent limiting plates (5) is equal to the width of the outside of the water storage box (7), the corner baffle (6) is L-shaped, and the inner side surface of the corner baffle (6) is attached to the water storage box (7).
5. The device for separating and collecting the oocytes in the ovaries of the animal as claimed in claim 1, wherein the outer diameter of the connecting column (907) is equal to the width of the washing chute (905), and the bottom end of the push plate (908) is symmetrically embedded with balls.
6. The device for separating and collecting the oocytes in the ovaries of the animal according to claim 1, wherein the length of the limiting rod (1006) is equal to that of the first filler strip (1001).
7. The device for separating and collecting the high-efficiency oocyte of the ovary of the animal according to claim 1, wherein the length of the connecting rope (1108) is equal to that of the swinging rod (1102), and the sum of the lengths of the swinging rod (1102) and the vertical rod (1104) is equal to that of the bottom plate (1).
8. The device for separating and collecting the oocytes of the animal ovary of claim 1, wherein the sealing gasket (1107) is provided with anti-slip threads on the outer side surface, and the outer side surface of the sealing gasket (1107), one side surface of the swinging rod (1102) and one side surface of the vertical rod (1104) are all located in the same plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910260586.8A CN109852533B (en) | 2019-04-02 | 2019-04-02 | High-efficiency oocyte separation and collection device for animal ovary |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910260586.8A CN109852533B (en) | 2019-04-02 | 2019-04-02 | High-efficiency oocyte separation and collection device for animal ovary |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109852533A CN109852533A (en) | 2019-06-07 |
| CN109852533B true CN109852533B (en) | 2022-02-25 |
Family
ID=66902932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910260586.8A Active CN109852533B (en) | 2019-04-02 | 2019-04-02 | High-efficiency oocyte separation and collection device for animal ovary |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109852533B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2071294C1 (en) * | 1995-01-19 | 1997-01-10 | Маленко Галина Петровна | Device for isolating oocytes from ovaries of mammals |
| US6541243B1 (en) * | 2000-06-05 | 2003-04-01 | Axon Instruments, Inc. | Perfusion chamber for electrophysiological testing of oocytes |
| CN103289891A (en) * | 2013-04-23 | 2013-09-11 | 青海省畜牧兽医科学院 | Fast bovine oocyte collector |
| CN105838581A (en) * | 2016-05-11 | 2016-08-10 | 安徽农业大学 | Animal ovary oocyte separation and collection device |
| CN205660754U (en) * | 2016-04-21 | 2016-10-26 | 湖南人文科技学院 | No clear tape oocyte cutting knife |
| CN206173318U (en) * | 2016-09-27 | 2017-05-17 | 中国农业科学院特产研究所 | Ovum device is got to animal ovary |
| CN107034130A (en) * | 2017-06-20 | 2017-08-11 | 成都言行果科技有限公司 | A kind of patterning method collects egg mother cell device |
| CN206799620U (en) * | 2017-06-09 | 2017-12-26 | 中国农业科学院特产研究所 | A kind of patterning method collects egg mother cell device |
| CN208532791U (en) * | 2018-05-25 | 2019-02-22 | 成都艾伟孚生物科技有限公司 | A kind of acquisition mouse egg cell discloses egg apparatus |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1215280A1 (en) * | 2000-12-13 | 2002-06-19 | Vrije Universiteit Brussel | Method for in vitro culture of ovarian follicles |
-
2019
- 2019-04-02 CN CN201910260586.8A patent/CN109852533B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2071294C1 (en) * | 1995-01-19 | 1997-01-10 | Маленко Галина Петровна | Device for isolating oocytes from ovaries of mammals |
| US6541243B1 (en) * | 2000-06-05 | 2003-04-01 | Axon Instruments, Inc. | Perfusion chamber for electrophysiological testing of oocytes |
| CN103289891A (en) * | 2013-04-23 | 2013-09-11 | 青海省畜牧兽医科学院 | Fast bovine oocyte collector |
| CN205660754U (en) * | 2016-04-21 | 2016-10-26 | 湖南人文科技学院 | No clear tape oocyte cutting knife |
| CN105838581A (en) * | 2016-05-11 | 2016-08-10 | 安徽农业大学 | Animal ovary oocyte separation and collection device |
| CN206173318U (en) * | 2016-09-27 | 2017-05-17 | 中国农业科学院特产研究所 | Ovum device is got to animal ovary |
| CN206799620U (en) * | 2017-06-09 | 2017-12-26 | 中国农业科学院特产研究所 | A kind of patterning method collects egg mother cell device |
| CN107034130A (en) * | 2017-06-20 | 2017-08-11 | 成都言行果科技有限公司 | A kind of patterning method collects egg mother cell device |
| CN208532791U (en) * | 2018-05-25 | 2019-02-22 | 成都艾伟孚生物科技有限公司 | A kind of acquisition mouse egg cell discloses egg apparatus |
Non-Patent Citations (1)
| Title |
|---|
| 牛卵巢的保存与卵巢内卵母细胞采集的研究;王锋等;《西北农业学报》;20031222;第12卷(第4期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109852533A (en) | 2019-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107926290A (en) | Novel fruits picker | |
| CN208264770U (en) | A kind of automatic labeling label device | |
| CN108820306A (en) | A kind of suction egg device | |
| CN109852533B (en) | High-efficiency oocyte separation and collection device for animal ovary | |
| CN210042051U (en) | Monitoring equipment with waterproof function | |
| CN208082524U (en) | A kind of New Blood section medical tubes holder | |
| CN108583956A (en) | A kind of automatic bagging assembly apparatus for fishing net fishing line | |
| CN213650443U (en) | Self-cleaning glass processing is with putting things in good order device that has fixed establishment | |
| CN206895499U (en) | A kind of trough being easily installed | |
| CN216464239U (en) | Clamping mechanism for high polymer material composite film | |
| CN202878234U (en) | Functional key puller | |
| CN108422390A (en) | A kind of Furniture manufacture timber clamping device | |
| CN207714228U (en) | A kind of vacuum sweeper of adsorbable metal garbage | |
| CN203726084U (en) | Full automatic rubber plug installing system | |
| CN208079861U (en) | Novel fruits picker | |
| CN220052063U (en) | A cut device for food materials processing | |
| CN221069822U (en) | Caterpillar belt capable of removing dust | |
| CN215236458U (en) | Surface dust removal device for processing connecting plate | |
| CN208631153U (en) | A kind of photovoltaic string welding machine magazine | |
| CN216835798U (en) | Automatic feeding device is used in production of safe efficient PET sheet | |
| CN213194723U (en) | Cleaning device is used in timber apron rubber coating processing | |
| CN217281981U (en) | Indoor bridge structure for office network | |
| CN216468738U (en) | Device is decided to full-automatic light guide plate tectorial membrane | |
| CN205309438U (en) | Copper strips cutting holding moves material device | |
| CN210489636U (en) | Photovoltaic module is with clear limit device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231226 Address after: Room 511, Building B5-B, Shuntai Plaza, No. 2000 Shunhua Road, High tech Zone, Jinan City, Shandong Province, 250000 Patentee after: Shandong Olive Intelligent Technology Co.,Ltd. Address before: No. 6699, Qingdao Road, Huaiyin District, Jinan City, Shandong Province Patentee before: Shandong First Medical University (Shandong Academy of Medical Sciences) Effective date of registration: 20231226 Address after: No. 6699, Qingdao Road, Huaiyin District, Jinan City, Shandong Province Patentee after: Shandong First Medical University (Shandong Academy of Medical Sciences) Address before: 271010 No. 2, Yingsheng East Road, Tai'an City, Shandong Province Patentee before: TAISHAN MEDICAL University |
|
| TR01 | Transfer of patent right |