CN107034130A - A kind of patterning method collects egg mother cell device - Google Patents
A kind of patterning method collects egg mother cell device Download PDFInfo
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- CN107034130A CN107034130A CN201710468209.4A CN201710468209A CN107034130A CN 107034130 A CN107034130 A CN 107034130A CN 201710468209 A CN201710468209 A CN 201710468209A CN 107034130 A CN107034130 A CN 107034130A
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- Prior art keywords
- injection
- outer tube
- mother cell
- egg mother
- patterning method
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Mechanical Engineering (AREA)
- Cell Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
Egg mother cell device is collected the invention discloses a kind of patterning method, including injection body, one end of the injection body is provided with injection and pushes away seat, the other end of the injection body is provided with injection and imported and exported, what the injection was imported and exported is internally provided with cutting blade group, the outside that the injection is imported and exported is provided with absorption outer tube, the bottom for drawing outer tube is provided with absorption outer tube angle, the injection body is internally provided with injection putter, one end of the injection putter, which is provided with, cuts somebody's hair, entovarial egg cell and ovarian follicle are flowed in absorption outer tube from outer tube angle is drawn, then imported and exported by injection, injection import and export are internally provided with cutting blade group, gap between cutting blade group can pass through egg mother cell, ovarian follicle is then cut set of blades and cut, so that a large amount of egg cells inside ovarian follicle are released, flowed into by the gap of cutting blade group in injection-tube body, it is achieved thereby that obtaining substantial amounts of egg mother cell, it is more efficient and convenient.
Description
Technical field
The invention belongs to biological cell extractive technique field, and in particular to a kind of patterning method collects egg mother cell device.
Background technology
The method of egg mother cell collection mainly has three kinds, injects body (1) suction method：For relatively large animal, such as
Syringe needle, is exactly pierced into ovarian follicle and sucks out liquor folliculi and egg mother cell in the lump, then carry out under the microscope by pig etc.
It is that can select the ovarian follicle of purpose size to screen advantages, is separated to the ovum of desired maturity, and injection pushes away seat (2) cutting
Method：For less animal, such as ovary is placed in culture dish by mouse, rabbit etc., is pierced ovary one by one with syringe needle
Broken, because of ovarian follicle, only about syringe needle is big, and ovarian follicle can be completely severed, and egg mother cell and liquor folliculi can come out in the lump again in microscope
The screening the method advantages of lower progress ibid are can to obtain substantial amounts of egg mother cell, and the ovum of various maturity is all present,
Cut somebody's hair (3) enzyme digestion：Special enzyme can be such that follicular rupture comes out so as to egg mother cell, but using patterning method when
Wait, there can be less ovarian follicle and not cut through, is just flowed into syringe, it has not been convenient to observed under the microscope.
The content of the invention
Egg mother cell device is collected it is an object of the invention to provide a kind of patterning method, to solve to carry in above-mentioned background technology
The problem of going out.
To achieve the above object, the present invention provides following technical scheme：A kind of patterning method collects egg mother cell device, including
Injection body, injection push away seat, cut somebody's hair, injection putter, sealing shroud, draw outer tube, injection and import and export, draw outer tube angle, cutting
Set of blades and absorption inner tube, one end of the injection body are provided with injection and push away seat, and the other end of the injection body is provided with
Injection is imported and exported, and what the injection was imported and exported is internally provided with cutting blade group, and the outside that the injection is imported and exported is provided with suction
Take outer tube, the absorption outer tube is internally provided with absorption inner tube, the absorption outer tube and draw the bottom of inner tube and be provided with suction
Take outer tube angle, the injection body is internally provided with injection putter, and one end of the injection putter, which is provided with, cuts somebody's hair, and
Cut somebody's hair and the top of seat is pushed away in injection, the other end of the injection putter is provided with sealing shroud, and sealing shroud is in injection body
Portion.
It is preferred that, hollow cylindrical structure in the injection-tube body.
It is preferred that, the sealing shroud is close together with injecting the inwall of body, constitutes sealing space.
It is preferred that, the cutting blade group is provided with orthogonal blade, and the gap between knife face is less than ovarian follicle
Diameter.
It is preferred that, the injection putter is connected through a screw thread with sealing shroud.
It is preferred that, it is described be injected into outlet diameter and draw inner tube internal diameter match.
Compared with prior art, the beneficial effects of the invention are as follows：After the present invention is installed, the absorption on outer tube will be drawn
In outer tube angle insertion ovary, then a hand pins injection and pushes away seat, one pull it is dynamic cut somebody's hair, drive injection putter of cutting somebody's hair is vertical
Motion upwards, the sealing shroud that the other end of injection putter is set is moved upwards vertically together with injection putter, entovarial ovum
Cell and ovarian follicle are flowed in absorption outer tube from outer tube angle is drawn, and are then imported and exported by injection, and internal set is imported and exported in injection
There is the gap between cutting blade group, cutting blade group can be by egg mother cell, ovarian follicle is then cut set of blades and cut so that ovarian follicle
Internal a large amount of egg cells are released, and are flowed into by the gap of cutting blade group in injection-tube body, break into two with one's hands absorption outer tube and
Inner tube is drawn, substantial amounts of egg cell is left on the tube wall for drawing outer tube and absorption inner tube, can further collect, it is achieved thereby that
Substantial amounts of egg mother cell is obtained, the present invention is scientific and reasonable for structure, safe and convenient to use.
Brief description of the drawings
Fig. 1 is positive structure diagram of the invention；
Fig. 2 is injection import and export mix schematic diagram of the invention；
Fig. 3 is injection outer tube cross section structure diagram of the invention；
In figure：1- injection body, 2- injection push away seat, 3- cut somebody's hair, 4- injection putters, 5- sealing shrouds, 6- draw outer tube, 7- note
Inject outlet, 8- and draw outer tube angle, 9- cutting blades group, 10- absorption inner tubes.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1, Fig. 2 and Fig. 3 are referred to, the present invention provides a kind of patterning method and collects egg mother cell device technique scheme：It is a kind of
Patterning method collects egg mother cell device, including injection body 1, injection push away seat 2,3, injection putter 4 of cutting somebody's hair, sealing shroud 5, draw it is outer
Pipe 6, injection import and export 7, draw outer tube angle 8, cutting blade group 9 and draw inner tube 10, and one end of injection body 1 is provided with note
Penetrate and push away seat 2, the other end of injection body 1 is provided with injection and imports and exports 7, and that injects import and export 7 is internally provided with cutting blade group
9, the outside that injection imports and exports 7 is provided with absorption outer tube 6, and that draws outer tube 6 is internally provided with absorption inner tube 10, draws outer tube 6
The bottom for drawing inner tube 10 is provided with absorption outer tube angle 8, that injects body 1 is internally provided with injection putter 4, injection putter
4 one end, which is provided with, cuts somebody's hair 3, and 3 tops for pushing away seat 2 in injection of cutting somebody's hair, and the other end of injection putter 4 is provided with sealing shroud 5, and
Sealing shroud 5 is in the inside of injection body 1.
It is preferred that, hollow cylindrical structure in injection body 1.
It is preferred that, sealing shroud 5 is close together with injecting the inwall of body 1, constitutes sealing space.
It is preferred that, cutting blade group 9 is provided with orthogonal blade, and the gap between knife face is straight less than ovarian follicle
Footpath.
It is preferred that, injection putter 4 is connected through a screw thread with sealing shroud 5.
It is preferred that, injection is imported and exported 7 diameters and matched with drawing the internal diameter of inner tube 10.
It is preferred that, draw and opened gap is provided with outer tube 6 and absorption inner tube 10, and absorption inner tube 10 is close to absorption
On the inwall of outer tube 6.
The present invention operation principle and use flow：It is after the present invention is installed, the absorption outer tube drawn on outer tube 6 is oblique
Mouthfuls 8 insert in ovaries, and then a hand pins injection and pushes away seat 2, one pull it is dynamic cut somebody's hair 3,3 drive injection putters 4 of cutting somebody's hair are vertical
Motion upwards, the sealing shroud 5 that the other end of injection putter 4 is set is moved as injection putter 4 is upward vertically together, entovarial
Egg cell and ovarian follicle are flowed in absorption outer tube 6 from outer tube angle 8 is drawn, and then import and export 7 by injection, injection is imported and exported in 7
The gap that portion is provided between cutting blade group 9, cutting blade group 9 can be by egg mother cell, and ovarian follicle is then cut set of blades 9 and cut
Open so that a large amount of egg cells inside ovarian follicle are released, flowed into by the gap of cutting blade group 9 in injection body 1, separately
Outside, outer tube will be drawn to remove, and will break into two with one's hands and draw outer tube 6 and draw inner tube 10, left on the tube wall for drawing outer tube 6 and absorption inner tube 10
Substantial amounts of egg cell, can further be collected, it is achieved thereby that obtaining substantial amounts of egg mother cell.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of patterning method collects egg mother cell device, including injection body (1), injection push away seat (2), cut somebody's hair (3), injection putter
(4), sealing shroud (5), absorption outer tube (6), injection import and export (7), draw outer tube angle (8), cutting blade group (9) and draw interior
Manage (10), it is characterised in that：One end of the injection body (1) is provided with injection and pushes away seat (2), described to inject the another of body (1)
One end is provided with injection and imports and exports (7), and what (7) were imported and exported in the injection is internally provided with cutting blade group (9), described to be injected into
The outside of outlet (7) is provided with absorption outer tube (6), and inner tube (10) is drawn in being internally provided with for the absorption outer tube (6), described to inhale
The bottom of outer tube (6) and absorption inner tube (10) is taken to be provided with absorption outer tube angle (8), the inside of the injection body (1) is set
Have injection putter (4), one end of the injection putter (4) is provided with cut somebody's hair (3), and cut somebody's hair (3) push away the upper of seat (2) in injection
Side, the other end of the injection putter (4) is provided with sealing shroud (5), and sealing shroud (5) is in the inside of injection body (1).
2. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The injection body
(1) hollow cylindrical structure in.
3. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The sealing shroud (5)
It is close together with the inwall of injection body (1), constitutes sealing space.
4. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The cutting blade group
(9) orthogonal blade, and diameter of the gap less than ovarian follicle between knife face are provided with.
5. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The injection putter
(4) it is connected through a screw thread with sealing shroud (5).
6. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The injection is imported and exported
(7) diameter matches with drawing the internal diameter of inner tube (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710468209.4A CN107034130A (en) | 2017-06-20 | 2017-06-20 | A kind of patterning method collects egg mother cell device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710468209.4A CN107034130A (en) | 2017-06-20 | 2017-06-20 | A kind of patterning method collects egg mother cell device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107034130A true CN107034130A (en) | 2017-08-11 |
Family
ID=59541731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710468209.4A Withdrawn CN107034130A (en) | 2017-06-20 | 2017-06-20 | A kind of patterning method collects egg mother cell device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107034130A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109852533A (en) * | 2019-04-02 | 2019-06-07 | 泰山医学院 | A kind of high efficiency egg mother cell of animal ovary separates and collects device |
-
2017
- 2017-06-20 CN CN201710468209.4A patent/CN107034130A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109852533A (en) * | 2019-04-02 | 2019-06-07 | 泰山医学院 | A kind of high efficiency egg mother cell of animal ovary separates and collects device |
| CN109852533B (en) * | 2019-04-02 | 2022-02-25 | 泰山医学院 | High-efficiency oocyte separation and collection device for animal ovary |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170811 |
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| WW01 | Invention patent application withdrawn after publication |