CN206799620U - A kind of patterning method collects egg mother cell device - Google Patents
A kind of patterning method collects egg mother cell device Download PDFInfo
- Publication number
- CN206799620U CN206799620U CN201720674291.1U CN201720674291U CN206799620U CN 206799620 U CN206799620 U CN 206799620U CN 201720674291 U CN201720674291 U CN 201720674291U CN 206799620 U CN206799620 U CN 206799620U
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- CN
- China
- Prior art keywords
- blade
- hole
- culture dish
- egg mother
- mother cell
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Links
- 235000013601 eggs Nutrition 0.000 title claims abstract description 26
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 26
- 238000000059 patterning Methods 0.000 title claims abstract description 14
- 238000005119 centrifugation Methods 0.000 claims abstract description 21
- 235000015108 pies Nutrition 0.000 claims description 3
- 210000001672 Ovary Anatomy 0.000 abstract description 19
- 239000007788 liquid Substances 0.000 abstract description 17
- 210000004027 cells Anatomy 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- 210000001519 tissues Anatomy 0.000 abstract description 4
- 241000973497 Siphonognathus argyrophanes Species 0.000 abstract description 2
- 210000000287 oocyte Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 108009000097 DNA Replication Proteins 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 210000004681 Ovum Anatomy 0.000 description 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 230000034004 oogenesis Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
Abstract
The utility model discloses a kind of patterning method to collect egg mother cell device, including culture dish and sterile centrifugation tube, blade hole is offered on culture dish, blade and lower blade are installed on the inside of blade hole, upper blade is located at the surface of lower blade, through hole is offered on upper blade and lower blade, the bottom of lower blade is provided with connection ring, and the bottom of connection ring is connected with sterile centrifugation tube；This patterning method collects egg mother cell device, by setting several sterile centrifugation tubes in the underface of culture dish, when ovary is cut, the sterile centrifugation tube mouth of pipe closes, after cutting multiple ovaries, culture dish is rinsed with operation liquid, open blade hole, the related egg mother cell of liquid is set to rinse, solve multiple ovaries to cut in same culture dish, a large amount of tissues cause nutrient solution liquid muddy, it is necessary to the problem of a large amount of liquid dilutings, on the other hand multiple ovary cuttings are solved, the problem of egg mother cell causes cell quantity to reduce can be taken away by throwing away ovary process.
Description
Technical field
Biotechnology technical field is the utility model is related to, especially a kind of patterning method collects egg mother cell device.
Background technology
Egg mother cell is the oogonium that meiosis is carried out in Process of oogenesis.It is divided into first oocyte, secondary
Level egg mother cell and ripe egg mother cell, they are that oogonium breaks up and produces after DNA replication dna division, subtracts for the first time respectively
Number division and the product of second meiotic division, the collection mode of egg mother cell are generally multiple ovaries and carried out in same culture dish
Cutting, a large amount of tissues cause nutrient solution liquid muddy, it is necessary to a large amount of liquid dilutings, while when carrying out multiple ovaries cuttings, throw away
Egg mother cell can be taken away by falling ovary process, cause cell quantity to reduce.
Utility model content
The purpose of this utility model is that providing a kind of patterning method collects egg mother cell device, possesses and avoids nutrient solution liquid
The advantages of muddy and guarantee cell quantity, to solve the problems mentioned in the above background technology.
To achieve the above object, the utility model provides following technical scheme：A kind of patterning method collects egg mother cell device,
Including culture dish and sterile centrifugation tube, the culture dish is in round pie, and blade hole is offered on culture dish, in the blade hole
Inner side is provided with blade and lower blade, and upper blade is located at the surface of lower blade, offered on upper blade and lower blade logical
Hole, the outer side edges of upper blade are fixedly connected with blade hole, and the inwall in blade hole is also connected with lower blade, and the bottom of lower blade is provided with
Connection ring, the connection ring are located at the outside of through hole, the lower blade of top connection of connection ring, and the bottom of connection ring is connected with sterile
Centrifuge tube, sterile centrifugation tube are located at the underface of culture dish.
As further program of the utility model：The through hole is symmetric, and the cross section of through hole is in sector.
As further program of the utility model：The blade hole is uniformly distributed on culture dish.
As further program of the utility model：The connection ring is oblique, and the opening of connection ring is towards inner side.
Compared with prior art, the utility model beneficial effect：This patterning method collects egg mother cell device, by cultivating
The underface of ware sets several sterile centrifugation tubes, when ovary is cut, sterile centrifugation tube mouth of pipe closure, and after cutting multiple ovaries,
Culture dish is rinsed with operation liquid, opens blade hole, makes the related egg mother cell of liquid rinse, solves multiple ovaries same
Culture dish is cut, and a large amount of tissues cause nutrient solution liquid muddy, it is necessary to the problem of a large amount of liquid dilutings, on the other hand solve multiple
Ovary is cut, and the problem of egg mother cell causes cell quantity to reduce can be taken away by throwing away ovary process.
Brief description of the drawings
Fig. 1 is overall structure diagram of the present utility model；
Fig. 2 is upper blade schematic top plan view of the present utility model；
Fig. 3 is upper blade schematic side view of the present utility model；
Fig. 4 is lower blade schematic side view of the present utility model；
Fig. 5 is blade hole of the present utility model schematic top plan view.
In figure：1- culture dishes；11- blades hole；2- sterile centrifugation tubes；The upper blades of 3-；Blade under 4-；5- through holes；6- connections
Ring.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Refer to Fig. 1-5, in the utility model embodiment, a kind of patterning method collects egg mother cell device, including culture dish 1
With sterile centrifugation tube 2, culture dish 1 is in round pie, offers blade hole 11 on culture dish 1, and blade hole 11 is manhole, blade
Hole 11 is uniformly distributed on culture dish 1, and the lower section in blade hole 11 is provided with sterile centrifugation tube 2, is provided with the inner side in blade hole 11
Upper blade 3 and lower blade 4, upper blade 3 are located at the surface of lower blade 4, through hole 5 are offered on upper blade 3 and lower blade 4,
Through hole 5 can be not only used for breathing freely, and through hole 5 also reduces deadweight, and blade 3 and lower blade 4 are used in high-speed rotation in reduction
Property, through hole 5 is in in distribution, the cross section of through hole 5 is in sector, and through hole 5 is very attractive in appearance, and the outer side edges of upper blade 3 are fixedly connected
Blade hole 11, the inwall in blade hole 11 are also connected with lower blade 4, and the bottom of lower blade 4 is provided with connection ring 6, and connection ring 6 is in incline
Ramp-like, the opening of connection ring 6 connects the top edge of sterile centrifugation tube 2, that is, descends blade 4 to pass through connection towards inner side, connection ring 6
Ring 6 connects sterile centrifugation tube 2, and connection ring 6 is located at the outside of through hole 5, and the top of connection ring 6 connects lower blade 4, connection ring 6
Bottom is connected with sterile centrifugation tube 2, and sterile centrifugation tube 2 is located at the underface of culture dish 1, this oocytes collection device, passed through
Several 50ml sterile centrifugation tubes 2 are set in the underface of culture dish 1, when ovary is cut, the mouth of pipe of sterile centrifugation tube 2 closure, cut
After cutting multiple ovaries, culture dish 1 is rinsed with operation liquid, opens blade hole 11, makes the related egg mother cell of liquid rinse.
In summary：This patterning method collects egg mother cell device, by set in the underface of culture dish 1 several 50ml without
Bacterium centrifuge tube 2, when ovary is cut, the mouth of pipe of sterile centrifugation tube 2 closure, after cutting multiple ovaries, culture dish is rinsed with operation liquid
1, blade hole 11 is opened, makes the related egg mother cell of liquid rinse, solves multiple ovaries and cut in same culture dish, largely
Tissue causes nutrient solution liquid muddy, it is necessary to the problem of a large amount of liquid dilutings, on the other hand solves multiple ovaries cuttings, throws away ovum
Nest process can take away the problem of egg mother cell causes cell quantity to reduce.
It is obvious to a person skilled in the art that the utility model is not limited to the details of above-mentioned one exemplary embodiment, and
And in the case of without departing substantially from spirit or essential attributes of the present utility model, it can realize that this practicality is new in other specific forms
Type.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this practicality is new
The scope of type limits by appended claims rather than described above, it is intended that the equivalency fallen in claim is contained
All changes in justice and scope are included in the utility model.Any reference in claim should not be considered as limitation
Involved claim.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (4)
1. a kind of patterning method collects egg mother cell device, including culture dish (1) and sterile centrifugation tube (2), it is characterised in that：It is described
Culture dish (1) is in round pie, and blade hole (11) is offered on culture dish (1), is provided with the inside of the blade hole (11)
Blade (3) and lower blade (4), upper blade (3) are located at the surface of lower blade (4), opened on upper blade (3) and lower blade (4)
Provided with through hole (5), the outer side edges of upper blade (3) are fixedly connected with blade hole (11), the inwall of blade hole (11) also with lower blade (4)
Connection, the bottom of lower blade (4) are provided with connection ring (6), and the connection ring (6) is located at the outside of through hole (5), connection ring (6)
The lower blade (4) of top connection, the bottom of connection ring (6) is connected with sterile centrifugation tube (2), and sterile centrifugation tube (2) is positioned at culture
The underface of ware (1).
2. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The through hole (5) is in
Symmetrical, the cross section of through hole (5) is in sector.
3. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The blade hole (11)
It is uniformly distributed on culture dish (1).
4. a kind of patterning method according to claim 1 collects egg mother cell device, it is characterised in that：The connection ring (6)
Obliquely, the opening of connection ring (6) is towards inner side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720674291.1U CN206799620U (en) | 2017-06-09 | 2017-06-09 | A kind of patterning method collects egg mother cell device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720674291.1U CN206799620U (en) | 2017-06-09 | 2017-06-09 | A kind of patterning method collects egg mother cell device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206799620U true CN206799620U (en) | 2017-12-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720674291.1U Expired - Fee Related CN206799620U (en) | 2017-06-09 | 2017-06-09 | A kind of patterning method collects egg mother cell device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206799620U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109852533A (en) * | 2019-04-02 | 2019-06-07 | 泰山医学院 | A kind of high efficiency egg mother cell of animal ovary separates and collects device |
-
2017
- 2017-06-09 CN CN201720674291.1U patent/CN206799620U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109852533A (en) * | 2019-04-02 | 2019-06-07 | 泰山医学院 | A kind of high efficiency egg mother cell of animal ovary separates and collects device |
| CN109852533B (en) * | 2019-04-02 | 2022-02-25 | 泰山医学院 | High-efficiency oocyte separation and collection device for animal ovary |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171226 Termination date: 20180609 |