CN218059011U

CN218059011U - Testicle sperm separator

Info

Publication number: CN218059011U
Application number: CN202221146055.XU
Authority: CN
Inventors: 余柯达; 师帅; 高辉; 柴娟; 毛佳婷; 包云
Original assignee: Jinhua People 's Hospital
Current assignee: Jinhua People 's Hospital
Priority date: 2022-05-13
Filing date: 2022-05-13
Publication date: 2022-12-16
Anticipated expiration: 2032-05-13

Abstract

The utility model relates to a testicle sperm separation device, which comprises a separation vessel and a cutting knife; the separation vessel comprises a vessel cover and a separation vessel main body; the separation vessel main body is of a circular structure; the separation vessel main body consists of an outer wall and an inner wall, and a water ring with a hollow structure and an opening at the upper end is formed between the outer wall and the inner wall; a working area is arranged at the bottom of the middle of the separation vessel main body; the testis sperm separation device has the advantages of convenience in separation, good separation effect and time saving.

Description

Testis sperm separation device

Technical Field

The utility model relates to a testicle sperm separator.

Background

Assisted reproduction technology is the most effective means for treating infertility, wherein intracytoplasmic sperm injection (ICSI) is used for treating infertility caused by male factors, particularly for patients with obstructive azoospermia, testicular sperm aspiration (TESA) is required to aspirate a small amount of seminiferous tubules from the testis, and the seminiferous tubules are shredded to obtain sperms for ICSI operation. At present, there is not the device of separating the sperm from testicular tissue on the market, the diameter 3.5 cm's culture dish is adopted in each big reproductive medicine center laboratory more, do a culture solution liquid drop in the middle part of the dish bottom, preheat to 37 ℃ after, put into the liquid drop with the thin seminal duct of the male sex patient that TESA obtained, reuse 2 syringe needles of 1ml syringe each other to cut the thin seminal duct, with tearing the thin seminal duct, cover the dish lid again and put into the incubator and cultivate for a period of time, so that more testicular sperms release in the liquid drop, absorb the liquid drop that contains the sperm at last in order to carry out the ICSI operation, avoid absorbing to the thin seminal duct piece of curved as far as possible. The method has low efficiency of cutting the seminiferous tubules, and the sperm suspension sucked by the method contains more fragments of the seminiferous tubules, which can cause adverse effects on ICSI outcome. Accordingly, there is a need for improvements in the art.

SUMMERY OF THE UTILITY MODEL

The utility model mainly solves the technical problems existing in the prior art, thereby providing a testis sperm separating device which is convenient for separation and has good separation effect and time saving.

The above technical problem of the present invention can be solved by the following technical solutions:

the utility model relates to a testicle sperm separation device, which comprises a separation vessel and a cutting knife; the separation vessel comprises a vessel cover and a separation vessel main body; the separation vessel main body is of a circular structure; the separation vessel main body consists of an outer wall and an inner wall, and a water ring with a hollow structure and an opening at the upper end is formed between the outer wall and the inner wall; the bottom in the middle of the separation ware main body is provided with a working area.

Preferably, the working area is a groove which is concave downwards on the bottom surface of the separation vessel main body.

Preferably, the working area comprises a cutting area and a liquid absorption area, and a filter screen is arranged between the cutting area and the liquid absorption area.

Preferably, the aperture of the filter screen is 1mm.

Preferably, fixed columns are arranged in the cutting area, and the number of the fixed columns is more than 2, and the fixed columns are uniformly distributed.

Preferably, the bottom of the liquid suction area is provided with a semicircular ring structure, and the diameter of the semicircular ring structure is 5mm.

Preferably, the cutting knife comprises a handle and a blade.

Preferably, a blade fixing frame is arranged between the knife handle and the blade.

Preferably, the number of the blades is more than 2, and the blades are uniformly arranged on the blade fixing frame.

The utility model has the advantages that:

the design of the water ring enables the separation vessel to contain distilled water, so that a saturated humidity environment is formed in the separation vessel; the cutting area is formed by a plurality of fixing columns, so that the fine bent pipe is easier to place and fix, and after the cutting area is matched with the upper cutting knife, the fine bent pipe can be accurately and quickly cut, the separation effect is achieved in a short time, and the separation effect is improved due to the fact that time is saved; the design of the filter screen enables the cut seminiferous tubules and sperms to be separated, so that fragments of the seminiferous tubules can be filtered out without manual careful operation, and further testicular sperms with few impurities can be obtained; can also improve the clinical effect of ICSI.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural view of the separation vessel of the present invention;

FIG. 2 is a schematic structural view of a separation vessel body;

FIG. 3 is an enlarged view of a portion of FIG. 2;

fig. 4 is a schematic structural view of the middle cutting knife of the present invention.

In the figure: 1-separation vessel, 2-cutting knife, 1.1-vessel cover, 1.2-outer wall, 1.3-water ring, 1.4-inner wall, 1.5-cutting area, 1.6-filter screen, 1.7-liquid suction area, 1.8-fixed column, 2.1-handle, 2.2-blade fixing frame, 2.3-blade

Detailed Description

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, and the scope of the present invention can be more clearly and clearly defined.

As shown in fig. 1-4, a testicle sperm separation device is mainly used for tearing a seminiferous tubule extracted from a testicle, thereby separating sperms. Comprises a separation vessel 1 and a cutting knife 2, wherein the separation vessel 1 and the cutting knife 2 are matched and combined for use; the separation vessel 1 comprises a vessel cover 1.1 and a separation vessel main body; the dish cover 1.1 can movably cover the upper part of the separation dish main body; the separation vessel main body is of a circular structure; the separation vessel main body consists of an outer wall 1.2 and an inner wall 1.4, and a water ring 1.3 with a hollow structure and an open upper end is formed between the outer wall 1.2 and the inner wall 1.4 and is used for containing distilled water; the bottom in the middle of the separation ware main body is provided with a working area.

The working area is a groove which is concave downwards on the bottom surface of the separation vessel main body.

The working area comprises a cutting area 1.5 and a liquid suction area 1.7, and a filter screen 1.6 is arranged between the cutting area 1.5 and the liquid suction area 1.7.

The aperture of the filter screen 1.6 is 1mm.

Fixed columns 1.8 are arranged in the cutting area 1.5, and the number of the fixed columns 1.8 is more than 2, and the fixed columns are uniformly distributed.

And a semicircular ring structure is arranged at the bottom of the liquid absorption area 1.7, and the diameter of the semicircular ring structure is 5mm.

The cutting knife 2 comprises a knife handle 2.1 and a blade 2.3.

A blade fixing frame 2.2 is arranged between the knife handle 2.1 and the blade 2.3.

The blades 2.3 are more than 2 in total and are uniformly arranged on the blade fixing frame 2.2. In particular, the number of blades 2.3 is matched to the distance between them and the number of fixing posts 1.8. The blade 2.3 can be inserted between the fixing posts 1.8.

When the device is actually used, the following work is carried out in advance: sterile distilled water was added to the water loop 1.3, and Serum replacement (SSS, irvine scientific, usa) and gamete prep (Modified HTF Medium, mHTF, irvine scientific, usa) were added to the working area at a ratio of 1:9, covering the dish cover with 1.1, and placing the mixture into an incubator at 37 ℃ for balancing for 30 minutes. Preheating to 37 ℃, opening the dish cover 1.1, placing the seminiferous tubules of male patients obtained by TESA into a cutting area 1.5, specifically into an area formed by fixed columns 1.8, placing a blade 2.3 of a cutting knife 2 into an interval between the fixed columns 1.8, cutting back and forth, covering the dish cover 1.1 after cutting, placing the dish cover into a 37 ℃ incubator for culturing for 2 hours, inclining the separation dish main body, enabling the sperm suspension containing the seminiferous tubule fragments to pass through a filter screen 1.6, filtering the seminiferous tubule fragments, enabling the sperm suspension to flow into a liquid suction area 1.7, sucking the sperm suspension from the liquid suction area 1.7, and using for ICSI.

The utility model has the advantages that:

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "the other end" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element to which the reference is made must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be either fixedly connected or detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases by those skilled in the art

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A testis sperm separation device is characterized in that: comprises a separation vessel (1) and a cutting knife (2); the separation dish (1) comprises a dish cover (1.1) and a separation dish main body; the separation vessel main body is of a circular structure; the separation vessel body consists of an outer wall (1.2) and an inner wall (1.4), and a water ring (1.3) with a hollow structure and an opening at the upper end is formed between the outer wall (1.2) and the inner wall (1.4); the bottom in the middle of the separation ware main body is provided with a working area.

2. A testicular sperm separation apparatus according to claim 1 wherein: the working area is a groove which is concave downwards on the bottom surface of the separation vessel main body.

3. A testicular sperm cell separation apparatus according to claim 2 wherein: the working area comprises a cutting area (1.5) and a liquid suction area (1.7), and a filter screen (1.6) is arranged between the cutting area (1.5) and the liquid suction area (1.7).

4. A testicular sperm separation apparatus according to claim 3 wherein: the diameter of the filter screen (1.6) is 1mm.

5. A testicular sperm separation apparatus according to claim 3 wherein: fixed columns (1.8) are arranged in the cutting area (1.5), and the number of the fixed columns (1.8) is more than 2, and the fixed columns are uniformly distributed.

6. A testicular sperm separation apparatus according to claim 4 wherein: and a semicircular ring structure is arranged at the bottom of the liquid suction area (1.7), and the diameter of the semicircular ring structure is 5mm.

7. A testicular sperm separation apparatus according to claim 1 wherein: the cutting knife (2) comprises a knife handle (2.1) and a blade (2.3).

8. A testicular sperm separation apparatus according to claim 7 wherein: a blade fixing frame (2.2) is arranged between the knife handle (2.1) and the blade (2.3).

9. A testicular sperm cell separation apparatus according to claim 8 wherein: the number of the blades (2.3) is more than 2, and the blades are uniformly arranged on the blade fixing frame (2.2).