CN209791588U - Tissue culture solution changing tube - Google Patents
Tissue culture solution changing tube Download PDFInfo
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- CN209791588U CN209791588U CN201920477959.2U CN201920477959U CN209791588U CN 209791588 U CN209791588 U CN 209791588U CN 201920477959 U CN201920477959 U CN 201920477959U CN 209791588 U CN209791588 U CN 209791588U
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- fluid exchange
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Abstract
The utility model provides a liquid pipe is traded to tissue culture liquid, it is concrete, it includes to trade the liquid pipe: a main body portion having a hollow cylindrical structure with a leading end and a trailing end; the air suction port is connected with the tail end of the main body part and is used for being connected with a low-pressure system to suck liquid; the suction nozzle is connected with the front end of the main body part and is used for contacting with liquid and sucking the liquid; wherein, the maximum diameter of the opening of the suction nozzle is 5-12mm, and a filter is arranged above the suction nozzle. The utility model discloses a tissue culture solution trades liquid pipe can smoothly inhale the liquid in tissue piece adherence is cultivateed, reduces tissue piece damage, pollution and loss.
Description
Technical Field
The utility model relates to a biological assay instrument field, concretely relates to tissue culture liquid-changing tube.
Background
The primary culture of umbilical cord mesenchymal stem cells, placenta mesenchymal stem cells, amnion mesenchymal stem cells and the like generally adopts a tissue block adherence method, the mesenchymal stem cells extracted by the method are relatively pure, the obtained cell amount is relatively large, and the activity is good. During the process of cell adherent culture, liquid changing operation is needed, and two common liquid changing methods are adopted, wherein one method is to directly use a 10ml common pipette to suck partial liquid. The other method is that the culture solution is poured into a 50ml centrifuge tube, the tissue which is not firmly attached to the wall is also poured into the 50ml centrifuge tube, the liquid above the tissue is discarded after centrifugation, the tissue is poured into a culture bottle for continuous culture, and the centrifuge tube centrifugation method has complex liquid changing operation, waste of consumables, disadvantages to batch operation, long exposure time and high pollution risk.
The operation is simple and flexible by using a suction tube (a pipette or a plastic pipette), the liquid is quickly transferred, but when the tissue culture solution is replaced, the suction nozzle is easily blocked by a tissue block (the tissue block for mesenchymal stem cell adherent culture is generally 3-10mm3Size), change the straw midway and make complex operation and cause tissue piece damage and increase the pollution probability, and inhale the tissue piece easily and cause the loss, consequently need one kind urgently to inhale smoothly in tissue piece adherence is cultivateed, reduces tissue piece damage, pollution and the tissue culture liquid change tube of loss.
SUMMERY OF THE UTILITY MODEL
The utility model provides a can smoothly inhale liquid in tissue piece adherent culture, reduce tissue piece damage, pollute and the tissue culture liquid replacement tube of loss.
A tissue culture fluid changing tube, the changing tube comprising:
A main body portion having a hollow cylindrical structure with a leading end and a trailing end;
The air suction port is connected with the tail end of the main body part and is used for being connected with a low-pressure system to suck liquid;
The suction nozzle is connected with the front end of the main body part and is used for contacting with liquid and sucking the liquid;
Wherein, the maximum diameter of the opening of the suction nozzle is 5-12mm, and a filter is arranged above the suction nozzle.
In another preferred embodiment, the maximum diameter of the opening is 7-10 mm.
In another preferred embodiment, the nozzle opening is circular and has a diameter equal to the inner diameter of the main body.
In another preferred example, the nozzle opening is a slit having a length equal to the inner diameter of the main body.
In another preferred embodiment, the slit width is 0.1-0.3 mm.
In another preferred example, the filter is a porous sieve plate, and the porous sieve plate is arranged in the liquid exchange tube and is 5-20mm away from the opening of the suction nozzle.
In another preferred embodiment, the diameter of the holes of the sieve plate is 0.1-2 mm.
In another preferred embodiment, the diameter of the holes of the sieve plate is 0.1-1 mm.
In another preferred embodiment, said screen plate has 2-20 of said holes.
In another preferred embodiment, the filter is a filter membrane filter.
in another preferred embodiment, the filter membrane of the filter membrane filter is selected from: polyvinylidene fluoride membranes, nylon membranes or nitrocellulose membranes.
in another preferred embodiment, the body portion is provided with a metrological scale.
In another preferred embodiment, the measurement scales comprise a positive scale and a negative scale, wherein the positive scale is arranged near the front end of the main body part, and the negative scale is arranged near the tail end of the main body part.
In another preferred example, the liquid exchange volume of the main body part is 2-50 mL.
In another preferred embodiment, the volume of the body part for replacing liquid is 5-20mL, preferably 10-15 mL.
In another preferred example, the liquid changing pipe is made of a plastic material or a glass material.
in another preferred example, a squeeze type air bag is further connected to the air inlet.
It is understood that within the scope of the present invention, the above-mentioned technical features of the present invention and those specifically described below (e.g. in the examples) can be combined with each other to constitute new or preferred technical solutions. Not to be reiterated herein, but to the extent of space.
Drawings
Fig. 1 is a schematic view of a liquid changing pipe according to embodiment 1 of the present invention.
the reference signs are:
1. A main body portion; 2. an air suction port; 3. a suction nozzle; 4. a perforated screen deck.
Detailed Description
The inventor of the invention develops a tissue culture solution liquid changing pipe through extensive and intensive research, prevents unsmooth liquid suction caused by blockage by enlarging an opening of a suction nozzle, and arranges a filter in the liquid changing pipe to intercept tissues, so that the liquid changing operation can be smoothly carried out, the operation times are reduced, the tissue block damage and pollution probability are reduced, the tissue loss during liquid changing is reduced, and the operation efficiency and yield are improved. And based on this, the utility model is completed.
as used herein, "tissue mass" and "tissue" are used interchangeably to refer to a multicellular tissue mass produced in adherent culture of the tissue mass.
Tissue culture solution changing tube
a tissue culture fluid changing tube, the changing tube comprising:
a main body portion having a hollow cylindrical structure with a leading end and a trailing end;
The air suction port is connected with the tail end of the main body part and is used for being connected with a low-pressure system to suck liquid;
The suction nozzle is connected with the front end of the main body part and is used for contacting with liquid and sucking the liquid;
Wherein, the maximum diameter of the opening of the suction nozzle is 5-12mm, and a filter is arranged above the suction nozzle.
In another preferred embodiment, the maximum diameter of the opening is 7-10 mm.
In another preferred embodiment, the nozzle opening is circular and has a diameter equal to the inner diameter of the main body.
in another preferred example, the nozzle opening is a slit having a length equal to the inner diameter of the main body. The slit has larger length, is not easy to be blocked completely, has certain filtering function and more convenient liquid absorption.
In another preferred embodiment, the slit width is 0.1-0.3 mm.
In another preferred example, the filter is a porous sieve plate, and the porous sieve plate is arranged in the liquid exchange tube and is 5-20mm away from the opening of the suction nozzle.
In another preferred embodiment, the diameter of the holes of the sieve plate is 0.1-2 mm.
in another preferred embodiment, the diameter of the holes of the sieve plate is 0.1-1 mm.
in another preferred embodiment, said screen plate has 2-20 of said holes.
In another preferred embodiment, the filter is a filter membrane filter.
In another preferred embodiment, the filter membrane of the filter membrane filter is selected from: polyvinylidene fluoride membranes, nylon membranes or nitrocellulose membranes.
In another preferred embodiment, the body portion is provided with a metrological scale.
In another preferred embodiment, the measurement scales comprise a positive scale and a negative scale, wherein the positive scale is arranged near the front end of the main body part, and the negative scale is arranged near the tail end of the main body part.
In another preferred example, the liquid exchange volume of the main body part is 2-50 mL.
In another preferred embodiment, the volume of the body part for replacing liquid is 5-20mL, preferably 10-15 mL.
In another preferred example, the liquid changing pipe is made of a plastic material or a glass material.
In another preferred example, a squeeze type air bag is further connected to the air inlet.
Advantages of the invention
1. The utility model discloses a trade liquid pipe through the transformation to the suction nozzle, has set up great suction nozzle opening for organize the piece and can not plug up whole suction nozzle opening when trading liquid, make and trade liquid operation and smoothly go on, be favorable to improving operating efficiency, save operating time, and reduce the tissue damage and the pollution that cause because of frequent operation.
2. The utility model discloses a trade liquid pipe filters through setting up the filter and holds back tissue piece, is favorable to reducing the tissue piece loss when trading liquid, can improve the yield.
The present invention will be further described with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
example 1
As shown in figure 1, the inner diameter of a main body part 1 of the liquid changing pipe is 7mm, the front end of the liquid changing pipe is connected with a suction nozzle 2, the opening of the suction nozzle 2 is equal to the inner diameter of the main body part 1, the tail end of the main body part 1 is connected with an air suction port 3, a porous sieve plate 4 is arranged 1cm above the suction nozzle 2, the porous sieve plate 4 is provided with a plurality of holes with the diameter of 0.2mm in a dispersed distribution manner, the whole liquid changing pipe is made of plastic materials and is integrally formed, positive and negative measurement scales are arranged on the surface of the whole liquid changing pipe, the positive scales are close to the suction nozzle, the negative scales are close to the air suction port, the positive scale range is 10mL, when in liquid suction, the air suction port 3 is connected with an ear washing ball, liquid is sucked from the suction nozzle, the opening of the suction nozzle is not blocked by tissue blocks, the problem that the liquid drops due to the, realize that the single operation is traded the liquid and is accomplished, the tissue damage that the at utmost leads to has reduced and traded the liquid, the pollution probability has been reduced, when the wall-pasting method culture solution trades the liquid, what often take is that the part trades the liquid, so can not suck the tissue culture solution dry, the negative scale can be very convenient instruct whether to have absorbed too much liquid, trade the liquid pipe imbibition back, there are a small amount of tissue pieces to hold back on porous sieve 4, can suitably loosen the induction port 3 at this moment and make the culture solution flow back a small amount of, make the tissue piece get back to the culture solution again, can reduce the tissue piece loss, the yield is improved.
Example 2
The difference between the embodiment 2 and the embodiment 1 is that the filter is a filter membrane filter which is changed from a porous sieve plate, and can be similar to a needle head type filter, only the filter membrane filter is not provided with a protruding part at an inlet and an outlet, the filter can be clamped in the liquid change pipe main body part 1, the filter membrane material can be selected from polyvinylidene fluoride (PVDF) membranes, nylon membranes or nitrocellulose membranes, and the like, the filter effect of the filter membrane is better, tissue blocks can be better intercepted, and the disposable sterile liquid change pipe with higher requirements is suitable.
All documents mentioned in this application are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
Claims (10)
1. A tissue culture broth changing tube, comprising:
A main body portion having a hollow cylindrical structure with a leading end and a trailing end;
The air suction port is connected with the tail end of the main body part and is used for being connected with a low-pressure system to suck liquid;
the suction nozzle is connected with the front end of the main body part and is used for contacting with liquid and sucking the liquid;
Wherein, the maximum diameter of the opening of the suction nozzle is 5-12mm, and a filter is arranged above the suction nozzle.
2. The fluid exchange tube of claim 1, wherein the nozzle opening is circular and has a diameter equal to an inner diameter of the main body portion.
3. The fluid exchange tube of claim 1, wherein the nozzle opening is a slit having a length equal to an inner diameter of the main body portion.
4. The fluid exchange tube of claim 1, wherein the filter is a perforated screen plate disposed within the fluid exchange tube between 5 mm and 20mm from the nozzle opening.
5. The drift tube of claim 4, wherein said screen plate holes have a diameter of 0.1-2 mm.
6. The drift tube of claim 4, wherein said screen panel has 2 to 20 of said holes.
7. The fluid exchange tube of claim 1, wherein the filter is a membrane filter.
8. The pipette of claim 7 wherein the filter membrane of the filter membrane filter is selected from the group consisting of: polyvinylidene fluoride membranes, nylon membranes or nitrocellulose membranes.
9. The fluid exchange tube of claim 1, wherein the main body portion is provided with a measurement scale, and the measurement scale comprises a positive scale and a negative scale, wherein the positive scale is provided near a front end of the main body portion, and the negative scale is provided near a rear end of the main body portion.
10. The fluid exchange tube of claim 1, wherein the body portion fluid exchange volume is 2-50 mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920477959.2U CN209791588U (en) | 2019-04-10 | 2019-04-10 | Tissue culture solution changing tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920477959.2U CN209791588U (en) | 2019-04-10 | 2019-04-10 | Tissue culture solution changing tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209791588U true CN209791588U (en) | 2019-12-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920477959.2U Active CN209791588U (en) | 2019-04-10 | 2019-04-10 | Tissue culture solution changing tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209791588U (en) |
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2019
- 2019-04-10 CN CN201920477959.2U patent/CN209791588U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221028 Address after: Floor 6, Building 1, No. 333 Guiping Road, Xuhui District, Shanghai Patentee after: CELLULAR BIOMEDICINE GROUP (SHANGHAI) Ltd. Address before: Room 417, 4 / F, tower 2, Lippo centre, 89 Queensway, Hong Kong, China Patentee before: CELLULAR BIOMEDICINE GROUP HK Ltd. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230907 Address after: North Zone, 1st Floor, Building 1, No. 159 Cailun Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area, Shanghai, 201210 Patentee after: Shibiman Biotechnology (Shanghai) Co.,Ltd. Address before: 200233 6th floor, building 1, 333 Guiping Road, Xuhui District, Shanghai Patentee before: CELLULAR BIOMEDICINE GROUP (SHANGHAI) Ltd. |
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| TR01 | Transfer of patent right | ||
| CP01 | Change in the name or title of a patent holder |
Address after: North Zone, 1st Floor, Building 1, No. 159 Cailun Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area, Shanghai, 201210 Patentee after: Shibiman Biotechnology (Shanghai) Co.,Ltd. Address before: North Zone, 1st Floor, Building 1, No. 159 Cailun Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area, Shanghai, 201210 Patentee before: Shibiman Biotechnology (Shanghai) Co.,Ltd. |
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| CP01 | Change in the name or title of a patent holder |