CN213012848U - Pipette convenient to remove tissue piece when primary cell passage - Google Patents
Pipette convenient to remove tissue piece when primary cell passage Download PDFInfo
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- CN213012848U CN213012848U CN202020979269.XU CN202020979269U CN213012848U CN 213012848 U CN213012848 U CN 213012848U CN 202020979269 U CN202020979269 U CN 202020979269U CN 213012848 U CN213012848 U CN 213012848U
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- pipette
- baffle
- suction nozzle
- pipe body
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Abstract
The utility model discloses a pipette convenient to get rid of tissue piece when primary cell passage aims at providing a pipette that can improve cell sap separation efficiency. It comprises a tube body on which scale marks are arranged; one end of the tube body is a suction nozzle end for sucking cell sap, and the other end of the tube body is a connecting end connected with a liquid shifter during use; a baffle is fixedly arranged on the pipe body and positioned at two sides of the suction nozzle end respectively; the two baffles are arranged in a V shape; the baffle is provided with a plurality of filtering holes for blocking tissue blocks and impurities; the suction nozzle end of the pipe body is bent to one side of the V-shaped bottom which is far away from the two baffles. The utility model discloses can effectively avoid the pipette to block up, improve the cell sap and get liquid efficiency.
Description
Technical Field
The utility model relates to a cell sap splitter technical field specifically is a pipette of getting rid of tissue piece when relating to a be convenient for primary cell passage.
Background
With the continuous development of stem cell technology, the demand for stem cells is increasing in the future. The method for obtaining the stem cells is divided into an enzyme digestion method and a tissue adherence method, and compared with the two methods, the method for obtaining the stem cells by adopting tissue adherence separation has simpler operation, less damage to the cells and higher safety. But when carrying out primary cell separation the tissue piece very easily blocks up the pipette, causes liquid to splash, and the suction nozzle end at absorption cell suspension in-process pipette constantly dodges the tissue, greatly reduced passage speed, still last the imbibition after the imbibition in-process if the tissue blocks up the pipette, because the too big liquid that leads to of suction pours into the pipettor into, the filter membrane contact liquid of pipettor can breed the bacterium, does not in time discover to cause cell pollution, and it is expensive to change the filter membrane moreover. Therefore, there is a need for improvements to existing pipettes.
SUMMERY OF THE UTILITY MODEL
The utility model aims at overcoming the defects existing in the prior art and providing a pipette which is convenient for removing tissue blocks when primary cells are passaged. The pipette can effectively avoid the blockage of the pipette and improve the efficiency of liquid taking of cell sap.
In order to solve the technical problem, the utility model discloses a realize through following technical scheme:
a pipette convenient for removing tissue blocks during primary cell passage comprises a tube body provided with scale marks; one end of the tube body is a suction nozzle end for sucking cell sap, and the other end of the tube body is a connecting end connected with a liquid shifter during use; a baffle is fixedly arranged on the pipe body and positioned at two sides of the suction nozzle end respectively; the two baffles are arranged in a V shape; the baffle is provided with a plurality of filtering holes for blocking tissue blocks and impurities; the suction nozzle end of the pipe body is bent to one side of the V-shaped bottom which is far away from the two baffles.
Specifically, the included angle between the two baffles is 60 degrees.
Specifically, the baffle and the pipe body are integrally formed into an integral piece.
Specifically, the baffle is connected with the pipe body in an adhering mode.
Specifically, the baffle is connected with the pipe body in a clamping mode.
Compared with the prior art, the utility model has the advantages of as follows:
the utility model discloses when carrying out the cell suspension separation, can effectively avoid tissue piece to block up the suction nozzle end of pipette to avoid liquid splash into the pipettor to pollute the filter core, the filtration pore on the pipette suction nozzle end baffle does benefit to liquid and filters out and absorb by the radian structure (crooked) of suction nozzle end, baffle and culture flask bottom laminating during the use, with the whole interception of tissue piece in pipette one side, this pipette not only can block tissue piece, 1-5mm3The solid substances can be intercepted, the sucked suspension does not contain impurities, the extraction efficiency of the cell suspension can be improved, and the tissue blocks and the impurities can be effectively prevented from entering the pipettor.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a bottom view of fig. 1.
Fig. 3 is an enlarged left-side view.
Detailed Description
For more clearly illustrating the objects, technical solutions and advantages of the present invention, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It is obvious that the embodiments described below are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by a person skilled in the art without any inventive work based on the embodiments described below belong to the scope of protection of the present invention.
In the description of the present invention, technical or scientific terms used should have the ordinary meaning as understood by those having ordinary skill in the art to which the present invention belongs. It should be noted that unless otherwise expressly stated or limited, the terms "mounted," "connected," "disposed," and the like are intended to be broadly construed and include, for example, fixedly connected, disposed, detachably connected, disposed, and integrally connected and disposed. The terms "connected" and similar terms are not limited to physical or mechanical connections, but may also include electrical connections, and those skilled in the art will understand the specific meanings of the above terms in the present invention according to specific situations. The terms "comprises" or "comprising," and the like, mean that the element or item preceding the term includes the element or item listed after the term and its equivalent, but not excluding other elements or items. The terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing and simplifying the present invention, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Meanwhile, in order to keep the following description of the embodiments of the present invention clear and concise, a detailed description of known functions and known parts is omitted.
As shown in the attached drawings, the pipette convenient for removing tissue blocks during the passage of primary cells comprises a tube body 2 provided with scale marks 1; one end of the tube body 2 is a suction nozzle end 3 for sucking cell sap, and the other end is a connecting end 4 connected with a liquid transfer device when in use; a baffle 5 is respectively fixedly arranged on the pipe body and positioned at the two sides of the suction nozzle end 3; the two baffles 5 are arranged in a V shape; the baffle 5 is provided with a plurality of filtering holes 6 for blocking tissue blocks and impurities; the suction nozzle end 3 of the tube body 2 is bent to one side of the V-shaped bottom which is far away from the two baffles 5.
In this embodiment, the included angle between the two baffles 5 is preferably 60 °, and obviously, the included angle can be increased or decreased appropriately. The baffle 5 and the pipe body 2 are preferably integrally formed into a whole. Obviously, the baffle 5 and the pipe body 2 can also be fixedly connected into a whole by adopting a bonding connection or a clamping connection after being manufactured in a split mode. The diameter of the filtering holes is preferably less than 1mm so as to intercept no less than 1mm3The solid substance of (1).
The utility model discloses increase two baffles at the integrative suction nozzle end of ordinary pipette, beaten the filtration pore on the baffle, the baffle can block along with the showy tissue piece of liquid, and the filtration pore of filtration on the baffle can accelerate liquid flow under the less condition of liquid, and the baffle is convenient for agree with the blake bottle bottom, prevents to organize to leak, and the head of pipette will have slight radian, and the liquid flow of being convenient for flows through, and the baffle position should be on the scale mark next door, is convenient for read the scale when inhaling liquid. The baffle and the pipette are made of the same material, and the baffle and the pipette can be formed at one time by using a mold without connection. In the use with liquid gathering in the blake bottle one corner, organize the piece and can sink to the bottle bottom very fast under the effect of gravity, support the pipette according to how much of organizing and have the position of certain distance apart from the bottle angle, along with liquid is less and less, organize the piece and can more and more gather to the baffle, liquid mainly flows through from the filtration pore on the baffle, and finally all organize the piece and are kept off in one side of pipette baffle, and the liquid of baffle opposite side is held the suction nozzle of pipette and is siphoned away smoothly.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. It should be understood by those skilled in the art that any modification, equivalent replacement, or improvement made to the technical solutions or parts of technical features described in the above embodiments can be included in the scope of the present invention within the spirit and principle of the present invention.
Claims (5)
1. A pipette convenient for removing tissue blocks during primary cell passage comprises a tube body (2) provided with scale marks (1); one end of the tube body (2) is a suction nozzle end (3) for sucking cell sap, and the other end is a connecting end (4) connected with a liquid transfer device when in use; the method is characterized in that: a baffle (5) is respectively fixedly arranged on the pipe body and positioned at the two sides of the suction nozzle end (3); the two baffles (5) are arranged in a V shape; a plurality of filtering holes (6) for blocking tissue blocks and impurities are formed in the baffle (5); the suction nozzle end (3) of the pipe body (2) is bent towards one side of the V-shaped bottom which is far away from the two baffles (5).
2. The pipette for facilitating removal of tissue mass upon passaging primary cells of claim 1, wherein: the included angle between the two baffle plates (5) is 60 degrees.
3. The pipette for facilitating the removal of tissue mass upon passaging primary cells according to claim 1 or 2, wherein: the baffle (5) and the pipe body (2) are integrally formed into a whole.
4. The pipette for facilitating the removal of tissue mass upon passaging primary cells according to claim 1 or 2, wherein: the baffle (5) is connected with the pipe body (2) in an adhesion mode.
5. The pipette for facilitating the removal of tissue mass upon passaging primary cells according to claim 1 or 2, wherein: the baffle (5) is connected with the pipe body (2) in a clamping manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020979269.XU CN213012848U (en) | 2020-06-02 | 2020-06-02 | Pipette convenient to remove tissue piece when primary cell passage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020979269.XU CN213012848U (en) | 2020-06-02 | 2020-06-02 | Pipette convenient to remove tissue piece when primary cell passage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213012848U true CN213012848U (en) | 2021-04-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020979269.XU Active CN213012848U (en) | 2020-06-02 | 2020-06-02 | Pipette convenient to remove tissue piece when primary cell passage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213012848U (en) |
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2020
- 2020-06-02 CN CN202020979269.XU patent/CN213012848U/en active Active
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