CN214694156U

CN214694156U - Stem cell filter for separating microcarrier

Info

Publication number: CN214694156U
Application number: CN202020866942.9U
Authority: CN
Inventors: 华子昂; 张建; 孙宁; 赵凯龙
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-18
Filing date: 2020-05-18
Publication date: 2021-11-12
Anticipated expiration: 2030-05-18

Abstract

The utility model provides a stem cell filter of separation microcarrier, including microcarrier filter plate, funnel, the integrative structure of collecting vessel composite, the funnel can only communicate through the filtration pore on the microcarrier filter plate with the collecting vessel. Can realize the rapid separation of the microcarrier and the stem cells.

Description

Stem cell filter for separating microcarrier

Technical Field

The utility model relates to a stem cell culture field especially relates to a stem cell filter of separation microcarrier.

Background

The stem cells have a variety of types and are widely applied in the fields of disease treatment, medical and beauty treatment, anti-aging, drug screening and the like. Stem cell culture has become a bottleneck for the development of the whole stem cell industry chain, and stem cell culture devices have become hot spots for the development of the stem cell culture field.

In the current stem cell culture device, insoluble microcarriers are used in the process of culturing adherent cells, and in the process of harvesting the cells, the adherent cells are separated from the microcarriers by pancreatin to form a microcarrier and cell mixed solution, and then the microcarriers and the cells are separated by a stem cell filter. At present, most laboratories adopt cell screens with the diameter of 2 cm to 3 cm, and a pipette is used for sucking mixed liquid of microcarriers and cells and transferring the mixed liquid onto the cell screens, so that the microcarriers are intercepted, and stem cells are harvested. The cell screen is influenced by the filtering area and the filtering performance, when the cell culture quantity is large, the consumption of the microcarrier is large, the filtering operation becomes complicated and complicated, the filtering time is long, and the pollution risk of stem cells is increased.

Disclosure of Invention

The utility model provides a stem cell filter of separation microcarrier, its essence is closely to combine funnel and receiving flask together through the microcarrier filter plate, guarantees that funnel and receiving flask can only pass through the filtration pore intercommunication on the microcarrier filter plate, reaches the purpose of separation stem cell and microcarrier, and traditional open system operation has been avoided to whole process simultaneously, and the quick separation that utilizes closed system to accomplish stem cell and microcarrier can also effectively prevent that the stem cell from receiving the pollution.

The utility model discloses a concrete technical scheme is: the funnel and the collecting bottle are respectively arranged on two sides of the micro-carrier filter plate, and the calibers of the micro-carrier filter plate, the funnel and the collecting bottle are matched, so that the funnel and the collecting bottle can be effectively combined together and can be ensured to be communicated only through the filter hole on the micro-carrier filter plate.

The microcarrier is separated by using a closed system, so that stem cell pollution possibly caused by the operation of the traditional open system can be avoided, and the microcarrier can not fall off from the microcarrier filter plate during large-scale separation.

The beneficial effects of the utility model are that simple structure easily makes, effectively reaches the purpose of filtering separation microcarrier to can prevent that stem cell from receiving the pollution in the course of separating.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of a stem cell filter for separating microcarriers

FIG. 2 is a schematic diagram showing a split structure of a stem cell filter for separating microcarriers

In the figure: funnel 1 microcarrier filter plate 2 collecting bottle 3 funnel interface 4 stem cell outflow port 5 pump interface 6

Detailed Description

The filter comprises a funnel 1, a microcarrier filter plate 2 and a collection bottle 3 which are assembled according to a graph 1, wherein a stem cell outflow port 5 of a stem cell filter for separating a microcarrier is closed, a funnel connector 4 is connected with a container filled with a mixed solution of the microcarrier and the stem cells through a pipeline, under the action of a vacuum pump connected with a pump connector 6, the mixed solution of the microcarrier and the stem cells is input into the funnel 1 through the pipeline, the stem cells are smaller than the filtration pore diameter of the microcarrier filter plate 2 and can enter the collection bottle 3 together with a filtrate, the microcarrier is larger than the filtration pore diameter of the microcarrier filter plate 2 and stops in the funnel 1 after being intercepted by the microcarrier filter plate 2, and the effective separation of the microcarrier and the stem cells is completed.

Method for removing stem cells: the funnel port 4 is clamped, the collection bottle 3 is slightly tilted to cover the stem cell outflow port 5 with the stem cell-containing liquid, and sterile gas is pumped from the pump port 6 to blow out the stem cells and transfer them to a sterile collection container.

The cleaning method of the microcarrier comprises the following steps: after stem cells in the stem cell filter are discharged, a stem cell outflow port 5 is connected with a microcarrier cleaning solution pipeline, cleaning solution is sucked into a collection bottle 3 through a pump interface 6 by using a pump, three interfaces of the stem cell filter are closed, the stem cell filter is inverted, and the cleaning solution enters a funnel 1 to clean microcarriers. After the cleaning is finished, the stem cell filter is placed right, and the cleaning solution returns to the collection bottle 3, so that the cleaning solution can be recovered.

Method for removing microcarriers: the mouth of the tube is placed into a designated container after the funnel interface 4 is clamped by a clamp, the stem cell filter is turned upside down, the clamp is opened after the funnel 1 is positioned below, a sterile cleaning solution pipeline is connected with the pump interface 6, the cleaning solution is conveyed to the funnel 1 by sterile air, and then the microcarrier can be transferred from the funnel 1 to the designated container.

The present embodiments are intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A stem cell filter for separating microcarriers, comprising: the device comprises a microcarrier filter plate, a funnel, a collection bottle, a funnel connector, a stem cell outlet and a pump connector; the micro-carrier filter plate, the funnel and the collecting bottle are combined into a tight connection structure, the stem cell outlet is positioned at the bottom end of the collecting bottle, and the pump interface is positioned in the middle of the collecting bottle.

2. The stem cell filter of claim 1, wherein the pore size of the filter plate is in the range of 1 μm to 1 mm.

3. The stem cell filter of claim 1, wherein the funnel is connected to the collection vial only through the filter holes of the filter plate of the microcarrier.

4. The stem cell filter for separating microcarrier according to claim 1, wherein the filter plate, the funnel and the collection bottle have matching apertures, and can be integrated or combined.