CN210711546U

CN210711546U - A mouth straw device for unicellular separation

Info

Publication number: CN210711546U
Application number: CN201921639568.2U
Authority: CN
Inventors: 牛冬; 汪滔
Original assignee: Nanjing Qizhen Genetic Engineering Co Ltd
Current assignee: Nanjing Qizhen Genetic Engineering Co Ltd
Priority date: 2019-09-29
Filing date: 2019-09-29
Publication date: 2020-06-09
Anticipated expiration: 2029-09-29

Abstract

The utility model discloses a mouth pipette device for unicellular is selected separately, including 200ml pipettor suction head, long rubber hose has been cup jointed in the outside of 200ml pipettor suction head, long rubber hose's inboard is provided with 0.45um filter sieve, 0.45um filter sieve is including the second connector with long rubber hose connection, short rubber hose has been cup jointed in the outside of first connector, short rubber hose's inboard is provided with the glass needle tubing, the glass needle tubing is including the third needle tubing with short rubber hose connection, the first needle tubing of one end fixedly connected with of second needle tubing. The utility model discloses can conveniently be sorted out the colony cell effectively for the unicellular, the cost can be ignored almost to can use repeatedly, the unicellular activity of sorting out with the mouth straw is higher than using flow cytometry sorter, especially to very fragile, hardly continuous culture's primary cell, and the advantage with the mouth straw is very big, can improve the survival rate of sorting out back primary cell greatly.

Description

A mouth straw device for unicellular separation

Technical Field

The utility model relates to the technical field of medical treatment, specifically be a mouth straw device for unicellular separation.

Background

In cell-related experiments, it is often necessary to sort cells from a population into single cells in order to study the function of single cells, or to sequence single cells, or to genetically edit a population of cells to obtain single cells with a particular editing state.

Although there are flow cytometers that can sort the population cells into single cells, the flow cytometers are extremely expensive, with values of millions of dollars, and their consumables are also very expensive. Most researchers do not have sufficient financial resources to purchase such large devices and the viability of primary cells sorted by the instrument is low. Therefore, it is desirable to design an oral pipette device for single cell sorting.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a mouth straw device for unicellular is selected separately, solved unicellular and selected separately the problem that relies on the extremely expensive flow cytometer of price, and the primary cell survival rate that this equipment was selected separately is low.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a mouth pipette device for unicellular separation, includes 200ml pipettor suction head, long rubber hose has been cup jointed in the outside of 200ml pipettor suction head, long rubber hose's inboard is provided with 0.45um filter sieve, 0.45um filter sieve is including the second connector of being connected with long rubber hose, the one end fixedly connected with middle part filter tube of second connector, the first connector of one end fixedly connected with of middle part filter tube, short rubber hose has been cup jointed in the outside of first connector, short rubber hose's inboard is provided with the glass needle tubing, the glass needle tubing is including the third needle tubing of being connected with short rubber hose, the one end fixedly connected with second needle tubing of third needle tubing, the first needle tubing of one end fixedly connected with of second needle tubing.

Preferably, first needle tubing, second needle tubing and third needle tubing are the integrated into one piece structure, the outer bore of first needle tubing is 0.2mm, just the length of first needle tubing is 25mm, the outer bore of second needle tubing is 3.5mm, just the length of second needle tubing is 20mm, the outer bore of third needle tubing is 8mm, just the inner bore of third needle tubing is 6.5mm, the length of third needle tubing 11 is 100 mm.

Preferably, first connector, middle part filter tube and second connector are the integrated into one piece structure, the external diameter of first connector is 7mm, just first connector internal diameter is 4mm, the length of first connector is 7mm, the diameter of middle part filter tube is 28mm, just the thickness of middle part filter tube is 5mm, the external diameter of second connector is 4mm, just the internal diameter of second connector is 2.5mm, the length of second connector is 12 mm.

Preferably, the outer diameter of the long rubber hose is 6mm, the inner diameter of the long rubber hose is 3.5mm, and the length of the long rubber hose is 1200 mm.

Preferably, the 200ml pipette tip has a length of 50mm, and the 200ml pipette tip has an inner and outer diameter of 0.6mm and 1mm at one end thereof, and an inner and outer diameter of 6.3mm and 7.3mm at the other end thereof.

Preferably, the inner and outer diameters of the short rubber hose are 6.5mm and 10mm, respectively, and the length of the short rubber hose is 120 mm.

Compared with the prior art, the beneficial effects of the utility model are as follows: the utility model discloses a mouth straw device can conveniently be sorted into the unicellular with the colony cell effectively, and the cost can be neglected almost (be less than 10 yuan) to can use repeatedly, the activity of the unicellular of manual sorting with the mouth straw is than using flow cytometry sorter higher moreover, especially to very fragile, difficult continuous culture's primary cell, and the advantage with the mouth straw is very big, can improve the survival rate of sorting back primary cell greatly.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic structural view of a 200ml pipette tip of the present invention;

FIG. 3 is a schematic structural view of the 0.45um filter sieve of the present invention;

fig. 4 is a schematic view of the glass needle tube of the present invention.

In the figure: 1. a long rubber hose; 2. 0.45um filter sieve; 3. 200ml pipette tips; 4. a short rubber hose; 5. a glass needle tube; 6. a first connector; 7. a middle filter tube; 8. a second connector; 9. a first needle tube; 10. a second needle tube; 11 third syringe.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1:

please refer to fig. 1-4, a mouth suction pipe device for single cell sorting, comprising a 200ml pipette tip 3, a long rubber hose 1 is sleeved outside the 200ml pipette tip 3, a 0.45um filter sieve 2 is arranged inside the long rubber hose 1, the 0.45um filter sieve 2 comprises a second connector 8 connected with the long rubber hose 1, one end of the second connector 8 is fixedly connected with a middle filter pipe 7, one end of the middle filter pipe 7 is fixedly connected with a first connector 6, a short rubber hose 4 is sleeved outside the first connector 6, a glass needle tube 5 is arranged inside the short rubber hose 4, the glass needle tube 5 comprises a third needle tube 11 connected with the short rubber hose 4, one end of the third needle tube 11 is fixedly connected with a second needle tube 10, and one end of the second needle tube 10 is fixedly connected with a first needle tube 9.

Referring to fig. 4, the first needle tube 9, the second needle tube 10 and the third needle tube 11 are integrally formed, the outer diameter of the first needle tube 9 is 0.2mm, the length of the first needle tube 9 is 25mm, the outer diameter of the second needle tube 10 is 3.5mm, the length of the second needle tube 10 is 20mm, the outer diameter of the third needle tube 11 is 8mm, the inner diameter of the third needle tube 11 is 6.5mm, and the length of the third needle tube 11 is 100 mm.

Referring to fig. 3, the first connector 6, the middle filter tube 7 and the second connector 8 are integrally formed, the outer diameter of the first connector 6 is 7mm, the inner diameter of the first connector 6 is 4mm, the length of the first connector 6 is 7mm, the diameter of the middle filter tube 7 is 28mm, the thickness of the middle filter tube 7 is 5mm, the outer diameter of the second connector 8 is 4mm, the inner diameter of the second connector 8 is 2.5mm, the length of the second connector 8 is 12mm, the cost is low, and the survival rate of primary cells after sorting can be greatly improved.

Referring to fig. 1, the outer diameter of the long rubber hose 1 is 6mm, the inner diameter of the long rubber hose 1 is 3.5mm, and the length of the long rubber hose 1 is 1200 mm.

Referring to fig. 2, the 200ml pipette tip 3 has a length of 50mm, and the 200ml pipette tip 3 has an inner diameter and an outer diameter of 0.6mm and 1mm at one end thereof, and the 200ml pipette tip 3 has an inner diameter and an outer diameter of 6.3mm and 7.3mm at the other end thereof.

Referring to fig. 1, the inner and outer diameters of the short rubber hose 4 are 6.5mm and 10mm, respectively, and the length of the short rubber hose 4 is 120 mm.

The working principle is as follows: during assembly, firstly, the long rubber hose 1 is sleeved on the outer side of the 200ml pipette tip 3, then the long rubber hose 1 is sleeved on the outer side of the second connector 8, so that the long rubber hose is firmly connected with the 0.45um filter sieve 2, and finally the short rubber hose 4 is respectively sleeved on the outer sides of the first connector 6 and the glass needle tube 5, so that the glass needle tube 5 and the 0.45um filter sieve 2 are firmly connected with the short rubber hose 4.

The control mode of the present application is automatically controlled by a controller, the control circuit of the controller can be realized by simple programming by those skilled in the art, and the present application is common knowledge in the field, and the present application mainly protects the mechanical device, so the control mode and the circuit connection are not explained in detail in the present application.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A oral pipette device for single cell sorting, comprising a 200ml pipette tip (3), characterized in that: a long rubber hose (1) is sleeved on the outer side of the 200ml pipette tip (3), the inner side of the long rubber hose (1) is provided with a 0.45um filter screen (2), the 0.45um filter screen (2) comprises a second connector (8) connected with the long rubber hose (1), one end of the second connector (8) is fixedly connected with a middle filter pipe (7), one end of the middle filtering pipe (7) is fixedly connected with a first connector (6), the outer side of the first connector (6) is sleeved with a short rubber hose (4), the inner side of the short rubber hose (4) is provided with a glass needle tube (5), the glass needle tube (5) comprises a third needle tube (11) connected with the short rubber hose (4), one end of the third needle tube (11) is fixedly connected with a second needle tube (10), one end of the second needle tube (10) is fixedly connected with a first needle tube (9).

2. The oral pipette device for single cell sorting according to claim 1, wherein: first needle tubing (9), second needle tubing (10) and third needle tubing (11) are the integrated into one piece structure, the outer mouth of first needle tubing (9) footpath is 0.2mm, just the length of first needle tubing (9) is 25mm, the outer mouth of second needle tubing (10) footpath is 3.5mm, just the length of second needle tubing (10) is 20mm, the outer mouth of third needle tubing (11) footpath is 8mm, just the inner mouth of third needle tubing (11) footpath is 6.5mm, the length of third needle tubing (11) is 100 mm.

3. The oral pipette device for single cell sorting according to claim 1, wherein: first connector (6), middle part filter tube (7) and second connector (8) are the integrated into one piece structure, the external diameter of first connector (6) is 7mm, just first connector (6) internal diameter is 4mm, the length of first connector (6) is 7mm, the diameter of middle part filter tube (7) is 28mm, just the thickness of middle part filter tube (7) is 5mm, the external diameter of second connector (8) is 4mm, just the internal diameter of second connector (8) is 2.5mm, the length of second connector (8) is 12 mm.

4. The oral pipette device for single cell sorting according to claim 1, wherein: the outer diameter of the long rubber hose (1) is 6mm, the inner diameter of the long rubber hose (1) is 3.5mm, and the length of the long rubber hose (1) is 1200 mm.

5. The oral pipette device for single cell sorting according to claim 1, wherein: the length of the 200ml pipette tip (3) is 50mm, the inner diameter and the outer diameter of one end of the 200ml pipette tip (3) are respectively 0.6mm and 1mm, and the inner diameter and the outer diameter of the other end of the 200ml pipette tip (3) are respectively 6.3mm and 7.3 mm.

6. The oral pipette device for single cell sorting according to claim 1, wherein: the inner and outer diameters of the short rubber hose (4) are respectively 6.5mm and 10mm, and the length of the short rubber hose (4) is 120 mm.