CN203290081U - Device for preparing schistosomulum in vitro - Google Patents

Abstract

The utility model belongs to the field of schistosomiasis research, and discloses a device for preparing schistosomulum in vitro. The device is composed of a cercaria throwing-into pool with a fixed eave, a schistosomulum receiving pool with a fixed eave and a liquid adding port, and open clamps used for fixation. The epidermis of a shaved mouse skin covers the schistosomulum receiving pool upwards, the cercaria throwing-into pool is placed on the rat skin, the open clamps fix the cercaria throwing-into pool and the schistosomulum receiving pool, the schistosomulum receiving pool is filled with cell culture fluid or isosmotic solutions, a newly-flowing schistosome cercaria is thrown into the cercaria throwing-into pool, then the cercaria falls into the schistosomulum receiving pool after penetrating through the mouse skin placed between the cercaria throwing-into pool and the schistosomulum receiving pool, and therefore a harmless schistosomulum sample is obtained in vitro.

Description

A kind of external device for preparing schistosomulum

Technical field

A kind of external device for preparing schistosomulum, belong to the blood fluke research field.

Background technology

Blood fluke cercaria, from after overflowing in the infectious oncomelania body, in case after people and animals touched the water body that contains blood fluke cercaria, cercaria drilled skin, becomes virgin worm, because of virgin worm in animal body, therefore virgin worm is difficult to obtain.The method that obtains at present virgin worm is mainly a large amount of cercarias of zoogenetic infection by experiment, with buffer solution, rinses the portal vein of animal; Or obtain virgin worm by physical method.The virgin worm rate of recovery of laboratory animal method is low, need the great many of experiments animal, and the virgin worm surface that obtains has the tissue of animal to be difficult to clean out, protein analysis to virgin worm produces serious interference, and in operating process, can not guarantee omnidistance aseptic condition, strengthen the chance that is contaminated by bacterial when virgin worm is cultivated; Physical preparation method can add the different degree of injury of the virgin worm of great achievement.This device falls into virgin worm reception tank, the virgin worm of external acquisition after cercaria being drilled be placed on cercaria to drop into the mouse skin between pond and virgin worm reception tank; The method whole process can be carried out sterile working, and virgin polypide table provides stable and aseptic schistosomulum sample for further experiment without animal tissue.

The utility model content

The purpose of this utility model is in order to solve the deficiency of prior art, and provide a kind of can obtain aseptic, without the device of the schistosomulum sample of other animal tissues.

The technical solution of the utility model: a kind of external device for preparing schistosomulum, this device drops into pond, mouse skin, cell culture fluid or isotonic solution filling opening by the cercaria with fixing eaves, with the fixing virgin worm reception tank of eaves, fixedly eaves and opening folder form;

By shaving mao mouse skin that mouse epidermis cortex makes progress, cover on the virgin worm reception tank with fixing eaves, cercaria is dropped into to pond to be placed on mouse skin, the fixedly eaves that cercaria is dropped into to the fixedly eaves in pond and virgin worm reception tank is clamping fixed with opening, virgin worm reception tank one side is equipped with cell culture fluid or isotonic solution filling opening, in virgin worm reception tank, is full of cell culture fluid or isotonic solution;

Cercaria, drop in pond and drop into new blood fluke cercaria of overflowing, cercaria drills to be placed on after cercaria is dropped into the mouse skin between pond and virgin worm reception tank and falls into virgin worm reception tank, thereby from the harmless schistosomulum sample of external acquisition.

The beneficial effects of the utility model: this device falls into virgin worm reception tank, external acquisition schistosomulum after blood fluke cercaria being drilled be placed on cercaria to drop into the mouse skin between pond and virgin worm reception tank; This process whole process can be carried out sterile working, and virgin polypide table provides stable and aseptic schistosomulum sample for further experiment without animal tissue.

The accompanying drawing explanation

Fig. 1 is structural representation of the present invention, 1, with the cercaria of fixing eaves, drop into pond, 2, mouse skin, 3, cell culture fluid or isotonic solution filling opening, 4, with the fixing virgin worm reception tank of eaves, 5, fixing eaves, 6, opening presss from both sides.

Embodiment

Embodiment 1

A kind of external device for preparing schistosomulum, this device drops into pond 1 by the cercaria with fixing eaves, mouse skin 2, cell culture fluid or isotonic solution filling opening 3, with the fixing virgin worm reception tank 4 of eaves, fixedly eaves 5 and opening press from both sides 6 compositions;

By shaving mao mouse skin 2 that mouse epidermis cortex makes progress, cover on the virgin worm reception tank 4 with fixing eaves, cercaria is dropped into to pond 1 to be placed on mouse skin 2, the fixedly eaves that cercaria is dropped into to the fixedly eaves in pond 1 and virgin worm reception tank 4 is fixing with opening folder 6, virgin worm reception tank 4 one sides are equipped with cell culture fluid or isotonic solution filling opening 3, in virgin worm reception tank 4, are full of cell culture fluid or isotonic solution;

To shave a mao mouse epidermis cortex upwards covers on virgin worm reception tank, cercaria is dropped into to pond to be placed on the mouse skin, by opening is clamping, determines cercaria and drop into pond and virgin worm reception tank, in virgin worm reception tank, be full of in cell culture fluid or isotonic solution, cercaria, drop in pond and drop into new effusion schistosoma japonicum cercariae, 37 ℃ of water-baths, cercaria drill to be placed on after cercaria is dropped into the mouse skin between pond and virgin worm reception tank and fall into virgin worm reception tank, can obtain the schistosomulum sample.

Claims (1)

1. external device for preparing schistosomulum, it is characterized in that this device drops into pond (1) by the cercaria with fixing eaves, mouse skin (2), cell culture fluid or isotonic solution filling opening (3), with the fixing virgin worm reception tank (4) of eaves, fixedly eaves (5) and opening folder (6) form;

By shaving mouse skin (2) that mao mouse epidermis cortex makes progress, cover on the virgin worm reception tank (4) with fixing eaves, cercaria is dropped into to pond (1) to be placed on mouse skin (2), the fixedly eaves that cercaria is dropped into to the fixedly eaves in pond (1) and virgin worm reception tank (4) is fixing with opening folder (6), virgin worm reception tank (4) one sides are equipped with cell culture fluid or isotonic solution filling opening (3), in virgin worm reception tank (4), are full of cell culture fluid or isotonic solution;

Cercaria, drop in pond and drop into new blood fluke cercaria of overflowing, cercaria drills to be placed on after cercaria is dropped into the mouse skin between pond and virgin worm reception tank and falls into virgin worm reception tank, thereby from the harmless schistosomulum sample of external acquisition.