CN103299961B - A kind of external device and method preparing schistosomulum - Google Patents
A kind of external device and method preparing schistosomulum Download PDFInfo
- Publication number
- CN103299961B CN103299961B CN201310209729.5A CN201310209729A CN103299961B CN 103299961 B CN103299961 B CN 103299961B CN 201310209729 A CN201310209729 A CN 201310209729A CN 103299961 B CN103299961 B CN 103299961B
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- cercaria
- reception tank
- pond
- virgin worm
- schistosomulum
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Abstract
The external device and method preparing schistosomulum, belongs to schistosomiasis research field.This device is dropped into pond by the cercaria with fixing eaves, is formed with the virgin worm reception tank of fixing eaves and filling opening and pressing from both sides for fixing opening.To shave a mao mouse epidermis cortex upwards covers on virgin worm reception tank, cercaria being dropped into pond is placed on mouse skin, cercaria input pond and virgin worm reception tank is determined by opening is clamping, cell culture fluid or isotonic solution is full of in virgin worm reception tank, drop in pond cercaria and drop into blood fluke cercaria of newly overflowing, cercaria falls into virgin worm reception tank after drilling the mouse skin be placed between cercaria input pond and virgin worm reception tank, thus obtains harmless schistosomulum sample from external.
Description
Technical field
The external device and method preparing schistosomulum, belongs to blood fluke research field.
Background technology
After blood fluke cercaria is overflowed in infectious oncomelania body, once after people and animals touch the water body containing blood fluke cercaria, cercaria drills skin, becomes virgin worm, because of virgin worm in animal body, therefore virgin worm is difficult to obtain.The method of the virgin worm of the current acquisition mainly a large amount of cercaria of zoogenetic infection by experiment, with the portal vein of wash buffer animal; Or obtain virgin worm by physical method.The virgin worm rate of recovery of laboratory animal method is low, need great many of experiments animal, and the virgin worm surface obtained has the tissue of animal to be difficult to clean out, serious interference is produced to the protein analysis of virgin worm, and omnidistance aseptic condition can not be ensured in operation, strengthen the chance be contaminated by bacterial when virgin worm is cultivated; Physical preparation method can add the different degree of injury of the virgin worm of great achievement.Virgin worm reception tank is fallen into, the virgin worm of external acquisition after the mouse skin that this device makes cercaria drill to be placed on cercaria to drop between pond and virgin worm reception tank; The method whole process can carry out sterile working, and virgin polypide table is without animal tissue, provides stable and aseptic schistosomulum sample to further experiment.
Summary of the invention
The object of the invention is the deficiency in order to solve prior art, and provide a kind of can obtain aseptic, without the device of the schistosomulum sample of other animal tissues.
Technical scheme of the present invention: a kind of external device preparing schistosomulum, this device drops into pond, mouse skin, cell culture fluid or isotonic solution filling opening by the cercaria with fixing eaves, with the virgin worm reception tank of fixing eaves, fixing eaves and opening folder composition;
Cover on the virgin worm reception tank with fixing eaves by shaving a mao mouse epidermis cortex mouse skin upwards, cercaria being dropped into pond is placed on mouse skin, fixing eaves opening cercaria being dropped into the fixing eaves in pond and virgin worm reception tank is clamping fixed, cell culture fluid or isotonic solution filling opening are equipped with in virgin worm reception tank side, are full of cell culture fluid or isotonic solution in virgin worm reception tank;
The method of schistosomulum is prepared with described device, the blood fluke cercaria dropping into new effusion is dropped in pond cercaria, cercaria falls into virgin worm reception tank after drilling the mouse skin be placed between cercaria input pond and virgin worm reception tank, thus obtains harmless schistosomulum sample from external.
Beneficial effect of the present invention: fall into virgin worm reception tank, external acquisition schistosomulum after the mouse skin that this device makes blood fluke cercaria drill to be placed on cercaria to drop between pond and virgin worm reception tank; This process whole process can carry out sterile working, and virgin polypide table is without animal tissue, provides stable and aseptic schistosomulum sample to further experiment.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention, 1, drop into pond, 2, mouse skin with the cercaria of fixing eaves, 3, cell culture fluid or isotonic solution filling opening, 4, virgin worm reception tank with fixing eaves, 5, fixing eaves, 6, opening folder.
Embodiment
Embodiment 1
The external device preparing schistosomulum, this device drops into pond 1, mouse skin 2, cell culture fluid or isotonic solution filling opening 3 by the cercaria with fixing eaves, with the virgin worm reception tank 4 of fixing eaves, fixing eaves 5 and opening folder 6 composition;
Cover on the virgin worm reception tank 4 with fixing eaves by shaving a mao mouse epidermis cortex mouse skin 2 upwards, cercaria being dropped into pond 1 is placed on mouse skin 2, the fixing eaves opening folder 6 of the fixing eaves and virgin worm reception tank 4 that cercaria are dropped into pond 1 is fixed, cell culture fluid or isotonic solution filling opening 3 are equipped with in virgin worm reception tank 4 side, are full of cell culture fluid or isotonic solution in virgin worm reception tank 4;
To shave a mao mouse epidermis cortex upwards covers on virgin worm reception tank, cercaria being dropped into pond is placed on mouse skin, cercaria input pond and virgin worm reception tank is determined by opening is clamping, be full of in cell culture fluid or isotonic solution in virgin worm reception tank, drop in pond cercaria and drop into schistosoma japonicum cercariae of newly overflowing, 37 DEG C of water-baths, cercaria falls into virgin worm reception tank after drilling the mouse skin be placed between cercaria input pond and virgin worm reception tank, can obtain schistosomulum sample.
Claims (2)
1. the external device preparing schistosomulum, it is characterized in that this device drops into pond (1) by the cercaria with fixing eaves, mouse skin (2), cell culture fluid or isotonic solution filling opening (3), with the virgin worm reception tank (4) of fixing eaves, fixing eaves (5) and opening folder (6) composition;
To shave on virgin worm reception tank (4) that mao mouse epidermis cortex mouse skin upwards (2) covers with fixing eaves, cercaria being dropped into pond (1) is placed on mouse skin (2), fixing eaves opening folder (6) of the fixing eaves and virgin worm reception tank (4) that cercaria are dropped into pond (1) is fixed, cell culture fluid or isotonic solution filling opening (3) are equipped with in virgin worm reception tank (4) side, are full of cell culture fluid or isotonic solution in virgin worm reception tank (4).
2. the method for schistosomulum is prepared with device described in claim 1, it is characterized in that dropping in pond cercaria the blood fluke cercaria dropping into new effusion, cercaria falls into virgin worm reception tank after drilling the mouse skin be placed between cercaria input pond and virgin worm reception tank, thus obtains harmless schistosomulum sample from external.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310209729.5A CN103299961B (en) | 2013-05-31 | 2013-05-31 | A kind of external device and method preparing schistosomulum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310209729.5A CN103299961B (en) | 2013-05-31 | 2013-05-31 | A kind of external device and method preparing schistosomulum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103299961A CN103299961A (en) | 2013-09-18 |
| CN103299961B true CN103299961B (en) | 2015-08-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310209729.5A Expired - Fee Related CN103299961B (en) | 2013-05-31 | 2013-05-31 | A kind of external device and method preparing schistosomulum |
Country Status (1)
| Country | Link |
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| CN (1) | CN103299961B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201514365U (en) * | 2009-10-29 | 2010-06-23 | 艾丹 | Bubble-free diffusion cell |
| CN201535769U (en) * | 2009-11-05 | 2010-07-28 | 山东医学高等专科学校 | External transdermal diffusion device |
| CN103048248A (en) * | 2012-12-11 | 2013-04-17 | 哈尔滨医科大学 | Diffusion cell device capable of adjusting capacity of receiving cell and effective diffusion area |
| CN202886235U (en) * | 2012-09-27 | 2013-04-17 | 苏州出入境检验检疫局检验检疫综合技术中心 | Transdermal absorption simulating device |
| CN203290081U (en) * | 2013-05-31 | 2013-11-20 | 江苏省血吸虫病防治研究所 | Device for preparing schistosomulum in vitro |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05178677A (en) * | 1991-12-26 | 1993-07-20 | Taisei Corp | Production of water permeable porous body |
| US20090064768A1 (en) * | 2007-03-13 | 2009-03-12 | Emad Alkhawam | Devices for Determining the Release Profile of Macromolecules and Methods of Using the Same |
-
2013
- 2013-05-31 CN CN201310209729.5A patent/CN103299961B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201514365U (en) * | 2009-10-29 | 2010-06-23 | 艾丹 | Bubble-free diffusion cell |
| CN201535769U (en) * | 2009-11-05 | 2010-07-28 | 山东医学高等专科学校 | External transdermal diffusion device |
| CN202886235U (en) * | 2012-09-27 | 2013-04-17 | 苏州出入境检验检疫局检验检疫综合技术中心 | Transdermal absorption simulating device |
| CN103048248A (en) * | 2012-12-11 | 2013-04-17 | 哈尔滨医科大学 | Diffusion cell device capable of adjusting capacity of receiving cell and effective diffusion area |
| CN203290081U (en) * | 2013-05-31 | 2013-11-20 | 江苏省血吸虫病防治研究所 | Device for preparing schistosomulum in vitro |
Non-Patent Citations (1)
| Title |
|---|
| 周述龙."蠕虫体外培养及在血吸虫研究上的应用".《国外医学(寄生虫病分册)》.1980,第97-103页. * |
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| CN103299961A (en) | 2013-09-18 |
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Granted publication date: 20150812 Termination date: 20160531 |