CN106366426A - Separation gel system for platelet-rich plasma (PRP) extraction and purification and preparation method thereof - Google Patents
Separation gel system for platelet-rich plasma (PRP) extraction and purification and preparation method thereof Download PDFInfo
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- CN106366426A CN106366426A CN201610771296.6A CN201610771296A CN106366426A CN 106366426 A CN106366426 A CN 106366426A CN 201610771296 A CN201610771296 A CN 201610771296A CN 106366426 A CN106366426 A CN 106366426A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L23/00—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers
- C08L23/02—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers not modified by chemical after-treatment
- C08L23/04—Homopolymers or copolymers of ethene
- C08L23/08—Copolymers of ethene
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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- C08L23/02—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers not modified by chemical after-treatment
- C08L23/04—Homopolymers or copolymers of ethene
- C08L23/08—Copolymers of ethene
- C08L23/0807—Copolymers of ethene with unsaturated hydrocarbons only containing more than three carbon atoms
- C08L23/0815—Copolymers of ethene with aliphatic 1-olefins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
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Abstract
The invention discloses a separation gel system for platelet-rich plasma (PRP) extraction and purification and a preparation method thereof and solves the problems of low purity and less recovery rate existing in an existing PRP extraction technology. The inert separation gel system disclosed by the invention comprises inert separation gel, nano-scale porous silica and modified white carbon black; the density range of the separation gel system is 1.045-1.08 g/cm<3>. The separation gel system for platelet-rich plasma (PRP) extraction and purification and the preparation method thereof, disclosed by the invention, have the advantage that under the situation that the better purity is achieved, the recovery rate of PRP can be greatly increased.
Description
Technical field
The present invention relates to clinical medicine and inspection field, it is related to a kind of separation gel system and in particular to a kind of rich platelet
Blood plasma extracts separation gel system with purification and preparation method thereof.
Background technology
In recent years the research with clinical treatment and medical and beauty treatment technology finds, containing hematoblastic blood plasma in a large number, i.e. rich blood
Platelet-poor plasma (platelet-rich plasma, prp), rich in tgf-1, pdgf-ab, igf-i, egf and vegf etc. growth because
Son.These somatomedin have the quick effect starting Endogenous Growth Factors, human body is had good promoting growth of cell and
Repair, may be used in clinical treatment and beauty treatment.Thus in existing clinical treatment and beauty treatment, using from autologous extraction blood
Liquid purification, collects prp, backs into patient skin or organization table by medical procedure such as intramuscular injection, surface coating, point of puncture notes
Face, is not only reached treatment, beauty treatment and the recovery medical function of its body, also can avoid the repulsion being occurred using ectosome blood product
Reaction.
But current clinical technology great majority are by gathering more blood preparation, by, under the conditions of aseptic experiment room, entering
Capable secondary or multiple centrifugation, layering, extraction, multiple repeatable operation, finally obtain the desired amount of platelet.This clinical manipulation handss
Method desired blood specimen amount is more, and repeatedly centrifugation is extracted, and the prp pollution rate of recovery is high, and potential safety hazard is big.
And in prior art can quickly, safety carries out the methods of prp collection and has that purity is low, few the asking of the response rate
Topic.
Content of the invention
The technical problem to be solved is: in prior art can quickly, safety carry out the mode side of prp collection
Method has that purity is low, the response rate is few, provides a kind of platelet rich plasma for blood taking tube solving the above problems to extract and pure
The separation gel system changed, and provide the preparation method of this separation gel system.
The present invention is achieved through the following technical solutions:
A kind of platelet rich plasma extracts the separation gel system with purification, including inertia separation gel, nanometer porous dioxy
SiClx and modified white carbon black；The density range of this separation gel system is 1.045～1.08g/cm3.
The present invention, by the setting of the cooperation between above-mentioned constituent and density, can be better achieved erythrocyte, thin in vain
Born of the same parents or the separation of its fragment, and then the purity that reclaims platelet rich plasma is effectively ensured, and effectively improve organic efficiency.
Further, described inertia separation gel is non-neighboring benzene class inertia separation gel.In the present invention, this non-neighboring benzene class inertia separates
Glue possesses biological safety from material selects, is evaluated by bio-safety, and has good biocompatibility, and then for originally
Invention provides a kind of safer and more effective biocompatible materialses, solves the biological peace that current conventional vacuum blood collection tube exists
Full potential problem.
Further, the addition of described nanometer porous silicon dioxide is the 0.5%～1.2% of separation gel system, modified
The addition of white carbon is the 8%～12% of separation gel system.
After the combination of above-mentioned each component, Fu Xue little can be greatly enhanced in the case of ensureing preferable purity
The organic efficiency of plate blood plasma, the response rate is greatly improved.
Preferably, described non-neighboring benzene class inertia separation gel includes polybutene and chlorinated paraffin -52.Described polybutene accounts for
The 72～76% of inertia separation gel, described chlorinated paraffin -52 accounts for the 24～28% of inertia separation gel.
By the setting of above-mentioned composition and proportioning, greatly can improve the response rate and purity, effect is very aobvious simultaneously
Write.
As one of which preferred scope, the density range of described separation gel system is 1.045～1.055g/cm3.At this
Density range interior energy is greatly enhanced purity, and the prp response rate also can reach more than 20% under this condition.
As another kind preferably value range, the density of described finished product is 1.065～1.08g/cm3.In this density range
Interior energy, in the case of ensureing preferable purity, is greatly enhanced the prp response rate, this response rate can reach more than 40%.
Preferably, the particle mean size of described nanometer porous silicon dioxide and modified white carbon black is 3000 mesh～5000 mesh.
A kind of platelet rich plasma extracts the preparation method with the separation gel system of purification, comprising:
Step one, inertia separation gel is prepared into the highly viscous fluid that viscosity is 300,000～600,000；
Step 2, add nanometer porous silicon dioxide and modified white carbon black in highly viscous fluid again, be ground and stir
Making density range after mixing is 1.045～1.08g/cm3Finished product.
Microgranule homogenizing can effectively be realized by above-mentioned physical technology method, and then the finished product effect made can be made more.
Further, the particle mean size of described nanometer porous silicon dioxide and modified white carbon black is 3000 mesh～5000 mesh,
The addition of described nanometer porous silicon dioxide is the 0.5%～1.2% of separation gel system, and the addition of modified white carbon black is
The 8%～12% of separation gel system.
Further, described milling time is more than or equal to 8h, and the number of operations of described grinding and stirring is more than or equal to 3 times.
The present invention compared with prior art, has such advantages as and beneficial effect:
1st, the present invention solves rich platelet preparation complicated at present and purification technique, and the cooperation of each component of the present invention
After can guarantee that safety non-pollution in the case of, preferably meet the requirement of purity and the response rate, in the situation reaching preferable purity
Under, it is greatly enhanced the response rate of prp；
2nd, the present invention can meet the medical treatment purposes different with beauty treatment mechanism by the setting of two kinds of different densities systems；Relatively
The prp purity that low density systems are realized is high, can effectively be satisfied with that dentistry, orthopaedics etc. is high to purity requirement and dose requirements are few from
The pouring-in treatment of body blood plasma；The prp response rate that higher density system is realized is high, can meet skin surgery many to dose requirements and pure
Spend less demanding autologous plasma appearance coating type treatment；
3rd, in prior art, the adjacent benzene class plasticiser that industry is usually used, the present invention have chosen non-neighboring benzene class system, possesses
Biological safety, has passed through biological property evaluation, avirulence.
Specific embodiment
For making the object, technical solutions and advantages of the present invention become more apparent, with reference to embodiment, the present invention is made
Further detailed description, the exemplary embodiment of the present invention and its explanation are only used for explaining the present invention, are not intended as to this
The restriction of invention.
Embodiment
A kind of platelet rich plasma extracts the separation gel system with purification, including inertia separation gel, nanometer porous dioxy
SiClx and modified white carbon black；Wherein, inertia separation gel is non-neighboring benzene class inertia separation gel, preferably polybutene and chlorination stone
Wax -52.
The density range of this separation gel system is 1.045～1.08g/cm3.By weight, nanometer porous silicon dioxide
Addition be the 0.5%～1.2% of separation gel system, the addition of modified white carbon black is the 8%～12% of separation gel system,
Polybutene accounts for the 72～76% of inertia separation gel, and chlorinated paraffin -52 accounts for the 24～28% of inertia separation gel.Described nanoscale is many
Hole silicon dioxide adopts the silicon dioxide of Degussa model r-974, and described modified white carbon black adopts the modification of model ts720
White carbon.
A kind of platelet rich plasma of the present invention extracts as follows with the concrete preparation method of the separation gel system of purification:
Step one, by polybutene and chlorinated paraffin -52 mixing, 12 hours～20 little with the stirring of agitator Direct/Reverse
When, it is prepared into the highly viscous fluid that viscosity is 300,000～600,000；Grind nanometer porous silicon dioxide and modified white carbon black respectively, make
Its granularity is between 3000 mesh～5000 mesh.
Step 2, add nanometer porous silicon dioxide and modified white carbon black in highly viscous fluid again, repeatedly ground
And stirring, milling time 8 hours every time, make finished product after being repeated 3 times.
The finished product preparing is added in conventional vacuum blood taking tube, every addition is 1～2g, selects in the present embodiment
For the vacuum test tube of 15*100 specification, every addition is 2g.And made using conventional vacuum blood taking tube technique there is this
The vacuum test tube of bright separation gel system, i.e. add the effective conventional medicinal anticoagulant of vacuum blood collection first, then assemble medicinal fourth
Base rubber plug, finally carries out radiation sterilization.
Table 1
In above-mentioned table 1, reference examples 1-2 are with the difference of example 1-6: each constituent all within value range, density
Outside value range；
Comparison 3-4 is with the difference of example 1-6: lacks nanometer porous silicon dioxide in each constituent respectively and changes
The interpolation of property white carbon；
Reference examples 5-6 are with the difference of example 1-6: each constituent and density are all outside value range；
Reference examples 7 are with the difference of example 1-6: each constituent all outside value range, but density value range it
Interior.
After the composition proportioning of above-mentioned table 1, prepare finished product, then choose 50 blood samples, every part of quantitative blood sampling
8ml.The finished product that each blood sample is respectively adopted in above-mentioned table 1 carries out separating-purifying, the purity peace obtaining after detection separating-purifying
All response rate.
Average recovery rate assay method: (in blood plasma, prp extracts volume/quantitation blood sampling volume) × 100%；
Method for detecting purity: on Conventional blood analyser, extract quantitative platelet rich plasma and counted, record red thin
Born of the same parents, leukocyte and its other patched cell quantity are simultaneously averaged.Calculating rich platelet purity=(sampling quantity-other cells are put down
All measure) × 100%.Testing result is as shown in table 2
Table 2
| Response rate % | Purity | |
| Example 1 | 23 | 100 |
| Example 2 | 31 | 98.5 |
| Example 3 | 40 | 93 |
| Example 4 | 48 | 88 |
| Example 5 | 35 | 94 |
| Example 6 | 36 | 97 |
| Reference examples 1 | 42 | 72 |
| Reference examples 2 | 18 | 99 |
| Reference examples 3 | 29 | 89 |
| Reference examples 4 | 31 | 84 |
| Reference examples 5 | 20 | 99 |
| Reference examples 6 | 46 | 75 |
| Reference examples 7 | 43 | 76 |
By the data in above-mentioned table 2, only by the group of the composition of the present invention, proportioning and density conditions
Close, purity and the response rate could be significantly improved simultaneously.
Above-described specific embodiment, has been carried out to the purpose of the present invention, technical scheme and beneficial effect further
Describe in detail, be should be understood that the specific embodiment that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, all any modification, equivalent substitution and improvement within the spirit and principles in the present invention, done etc., all should comprise
Within protection scope of the present invention.
Claims (10)
1. a kind of platelet rich plasma extracts the separation gel system with purification it is characterised in that including inertia separation gel, nanometer
Level porous silica and modified white carbon black；Described inertia separation gel is non-neighboring benzene class inertia separation gel, this separation gel system
Density range is 1.045～1.08 g/cm3.
2. a kind of platelet rich plasma according to claim 1 extract and purification separation gel system it is characterised in that institute
The addition stating nanometer porous silicon dioxide is the 0.5%～1.2% of separation gel system, and the addition of modified white carbon black is to separate
The 8%～12% of colloid system.
3. a kind of platelet rich plasma according to claim 1 extract and purification separation gel system it is characterised in that institute
State inertia separation gel and include polybutene and chlorinated paraffin -52.
4. a kind of platelet rich plasma according to claim 3 extract and purification separation gel system it is characterised in that institute
State that polybutene accounts for inertia separation gel 72～76%, described chlorinated paraffin -52 accounts for the 24～28% of inertia separation gel.
5. a kind of platelet rich plasma according to any one of Claims 1 to 4 extracts the separation gel system with purification, and it is special
Levy and be, the density range of described separation gel system is 1.045～1.055g/cm3.
6. a kind of platelet rich plasma according to any one of Claims 1 to 4 extracts the separation gel system with purification, and it is special
Levy and be, the density range of described separation gel system is 1.065～1.08g/cm3.
7. a kind of platelet rich plasma according to claim 1 and 2 extracts the separation gel system with purification, and its feature exists
In the particle mean size of described nanometer porous silicon dioxide and modified white carbon black is 3000 mesh～5000 mesh.
8. a kind of platelet rich plasma extracts the preparation method with the separation gel system of purification it is characterised in that including:
Step one, inertia separation gel is prepared into the highly viscous fluid that viscosity is 300,000～600,000；
Step 2, add nanometer porous silicon dioxide and modified white carbon black in highly viscous fluid again, after being ground and stirring
Making density range is 1.045～1.08 g/cm3Finished product.
9. a kind of platelet rich plasma according to claim 8 extracts the preparation method with the separation gel system of purification, its
It is characterised by, the particle mean size of described nanometer porous silicon dioxide and modified white carbon black is 3000 mesh～5000 mesh, described receive
The addition of meter level porous silica is the 0.5%～1.2% of separation gel system, and the addition of modified white carbon black is to separate colloid
The 8%～12% of system.
10. a kind of platelet rich plasma according to claim 8 or claim 9 extracts the preparation side with the separation gel system of purification
It is characterised in that described milling time is more than or equal to 8h, the number of operations of described grinding and stirring is more than or equal to 3 times method.
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| CN201610771296.6A CN106366426B (en) | 2016-08-30 | 2016-08-30 | A kind of platelet rich plasma extraction and the separation gel system purified and preparation method thereof |
| PCT/CN2017/095954 WO2018040847A1 (en) | 2016-08-30 | 2017-08-04 | Separation gel system for extracting and purifying platelet-rich plasma and preparation method therefor |
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| CN201610771296.6A CN106366426B (en) | 2016-08-30 | 2016-08-30 | A kind of platelet rich plasma extraction and the separation gel system purified and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018040847A1 (en) * | 2016-08-30 | 2018-03-08 | 成都瑞琦科技实业股份有限公司 | Separation gel system for extracting and purifying platelet-rich plasma and preparation method therefor |
| CN110064230A (en) * | 2019-05-31 | 2019-07-30 | 朱德新 | A kind of platelet rich plasma acquisition separation vessel |
| CN111468204A (en) * | 2020-06-08 | 2020-07-31 | 珠海朗泰生物科技有限公司 | Platelet-rich plasma preparation tube with controllable components and preparation method thereof |
| CN113717320A (en) * | 2020-05-25 | 2021-11-30 | 华熙生物科技股份有限公司 | Preparation method of platelet-rich plasma separation gel, obtained product and application |
| CN116715801A (en) * | 2023-06-30 | 2023-09-08 | 珠海朗泰生物科技有限公司 | PRP (platelet-derived polymer) separating gel with low cytotoxicity and preparation method and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114010659B (en) * | 2021-11-23 | 2023-09-29 | 新疆维吾尔自治区人民医院 | Preparation method of platelet-rich plasma |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102309870A (en) * | 2011-06-17 | 2012-01-11 | 上海科华检验医学产品有限公司 | Blood separation gel used for blood collection vessel and preparation method thereof |
| CN103333446A (en) * | 2013-06-21 | 2013-10-02 | 武汉曙天科技发展有限公司 | Serum separation gel and preparation method thereof |
| CN104262651A (en) * | 2014-09-23 | 2015-01-07 | 武汉辉斯特科技有限公司 | Preparation and application of auto-platelet-rich plasma thixotropic separating gel |
| CN104558354A (en) * | 2014-12-08 | 2015-04-29 | 南雄阳普医疗科技有限公司 | Platelet-rich plasma separation gel and platelet-rich plasma preparation method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070190148A1 (en) * | 2006-02-14 | 2007-08-16 | Peter Cronin | Gel compositions, apparatuses and fluid separation methods |
| CN106366426B (en) * | 2016-08-30 | 2019-08-20 | 成都瑞琦科技实业股份有限公司 | A kind of platelet rich plasma extraction and the separation gel system purified and preparation method thereof |
-
2016
- 2016-08-30 CN CN201610771296.6A patent/CN106366426B/en active Active
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2017
- 2017-08-04 WO PCT/CN2017/095954 patent/WO2018040847A1/en active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102309870A (en) * | 2011-06-17 | 2012-01-11 | 上海科华检验医学产品有限公司 | Blood separation gel used for blood collection vessel and preparation method thereof |
| CN103333446A (en) * | 2013-06-21 | 2013-10-02 | 武汉曙天科技发展有限公司 | Serum separation gel and preparation method thereof |
| CN104262651A (en) * | 2014-09-23 | 2015-01-07 | 武汉辉斯特科技有限公司 | Preparation and application of auto-platelet-rich plasma thixotropic separating gel |
| CN104558354A (en) * | 2014-12-08 | 2015-04-29 | 南雄阳普医疗科技有限公司 | Platelet-rich plasma separation gel and platelet-rich plasma preparation method |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018040847A1 (en) * | 2016-08-30 | 2018-03-08 | 成都瑞琦科技实业股份有限公司 | Separation gel system for extracting and purifying platelet-rich plasma and preparation method therefor |
| CN110064230A (en) * | 2019-05-31 | 2019-07-30 | 朱德新 | A kind of platelet rich plasma acquisition separation vessel |
| CN113717320A (en) * | 2020-05-25 | 2021-11-30 | 华熙生物科技股份有限公司 | Preparation method of platelet-rich plasma separation gel, obtained product and application |
| CN113717320B (en) * | 2020-05-25 | 2024-02-02 | 华熙生物科技股份有限公司 | Preparation method of platelet-rich plasma separation gel, and obtained product and application thereof |
| CN111468204A (en) * | 2020-06-08 | 2020-07-31 | 珠海朗泰生物科技有限公司 | Platelet-rich plasma preparation tube with controllable components and preparation method thereof |
| CN116715801A (en) * | 2023-06-30 | 2023-09-08 | 珠海朗泰生物科技有限公司 | PRP (platelet-derived polymer) separating gel with low cytotoxicity and preparation method and application thereof |
| CN116715801B (en) * | 2023-06-30 | 2024-02-23 | 珠海朗泰生物科技有限公司 | PRP (platelet-derived polymer) separating gel with low cytotoxicity and preparation method and application thereof |
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| WO2018040847A1 (en) | 2018-03-08 |
| CN106366426B (en) | 2019-08-20 |
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