CN202892429U - Blood bag for density gradient centrifugation of mononuclear cells - Google Patents
Blood bag for density gradient centrifugation of mononuclear cells Download PDFInfo
- Publication number
- CN202892429U CN202892429U CN 201220582266 CN201220582266U CN202892429U CN 202892429 U CN202892429 U CN 202892429U CN 201220582266 CN201220582266 CN 201220582266 CN 201220582266 U CN201220582266 U CN 201220582266U CN 202892429 U CN202892429 U CN 202892429U
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- blood bag
- blood
- density gradient
- gradient centrifugation
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Abstract
A blood bag for density gradient centrifugation of mononuclear cells comprises a blood bag body and an outer packaging bag. A round hole for hanging is arranged on the upper portion of the blood bag body, the volume of the blood bag body is 280ml-320ml, the upper portion of the blood bag body is connected with two short tubes with rubber seals and screw caps, the lower portion of the blood bag body is connected with two long tubes with needle heads, fluid flow speed control valves are arranged on the long tubes, needle caps capable of being fastened down repeatedly are arranged on the needle heads, and the outer packaging bag is 28cm-32cm longer than the blood bag body. The blood bag for density gradient centrifugation of the mononuclear cells is low in manufacture cost, easy to operate, high in capacity and low in infection possibility.
Description
Technical field
This utility model relates to a kind of blood bag, especially relates to a kind of blood bag that separates mononuclearcell for density gradient centrifugation.
Background technology
At present, the used blood bag of scientific research institutions and hospital comprises blood bag body, the short tube of several sealings is equipped with on blood bag body top, the bottom is equipped with one with the long tube of syringe needle, syringe needle is with the disposable aspiration needle cover, in the bag 28 milliliters anticoagulant for storage of whole blood is housed generally, it contains anticoagulant and the hemotrophic nutrition agent such as citric acid, sodium citrate, glucose, sodium dihydrogen phosphate, adenine, this blood bag is mainly used to store blood in the blood bank and separating umbilical blood stem cell etc., preserves the time of blood and grows (about 35 days).
But, in numerous scientific research institutions and hospital, get blood, many times be for the method by density gradient centrifugation within a few hours obtains wherein mononuclearcell, and further cultivate or sorting.Yet, there is following defective in existing blood bag: it is many that (1) separates the follow-up consumptive material of mononuclearcell, cost of manufacture is high, easily pollute: during operation, mononuclearcell needs first one times of hemodilution just can be obtained with density－gradient centrifuga－tion method is centrifugal afterwards, take 100ml blood as example, need at least multiplex 4 50ml centrifuge tubes and 2 pipets to come dilute blood and add blood to the Ficoll fluid level; (2) be not suitable for separation and the cultivation of endothelial progenitor cells, when umbilical cord blood endothelial progenitor cells was cultivated, if with the sodium citrate anticoagulant in the existing blood bag, the endothelial progenitor cells that obtains was cloned less than 1/10th of anticoagulant heparin; At present, the umbilical blood that needs in the test to use anticoagulant heparin can only add heparin voluntarily in the 50ml centrifuge tube, take 100ml blood as example, need to add 6 50ml centrifuge tubes and 2 pipets just can be finished experiment; (3) get blood and easily pollute, the entry needle headgear of existing blood bag is normally disposable, and common pinhead sleeve can not go back by tight cover after the nurse got the blood operation, very easily polluted; If get blood with centrifuge tube, nurse operation is loaded down with trivial details and difficulty is large, during operation, needs the nurse to get in the centrifuge tube with the syringe blood drawing again; (3) capacity is little, and the most of volume of present used extraction umbilical blood blood bag is removed the shared volume of anticoagulant at 200ml, can only extract about 150ml umbilical blood, does not have enough volumes to hold diluent with volume; If use centrifuge tube, the blood of getting is less than 40ml usually, far can not satisfy the hematopoietic stem cell sorting needs at the common CD34+ of laboratory; (4) only have one to be used for blood drawing with the long tube of syringe needle, then can only be with cutting off the part long tube behind the iodine disinfection long tube during blood-letting in case the possible pollution in syringe needle position and avoid long tube and a small amount of blood clot of syringe needle.
The utility model content
Technical problem to be solved in the utility model is, overcomes the defects that prior art exists, and provides a kind of easy to operate simple, and possibility of pollution is low, and capacity is large, and the use procedure consumptive material is few can be used for the blood bag that density gradient centrifugation is separated mononuclearcell.
The technical scheme that its technical problem that solves this utility model adopts is, a kind of blood bag that separates mononuclearcell for density gradient centrifugation, comprise blood bag body, described blood bag body top is provided with one for the circular hole that hangs, described blood bag volume is 280ml-320ml, described blood bag body top is connected with two all with the short tube of rubber seal and screw lid, described blood bag body bottom is connected with two all with the long tube of syringe needle, described long tube is provided with the fluid-flow rate control valve, and described syringe needle is provided with the pin medicated cap that can repeat to cover tightly; Also be provided with outer packaging bag, described outer packaging bag is than the long 28cm-32c of blood bag body; Outer packaging bag is provided with tearing port.
Further, the preferred 300ml of described this body bulk of blood bag.
Further, described short tube length is 1.5cm-2.5cm.
Further, the preferred 2cm of described short tube length.
Further, described long tube length is 1cm-1.5cm.
Further, the preferred 1.2m of described long tube length.
Further, the described outer packaging bag length preferred 30cm longer than blood bag body.
Further, the tearing port on the outer packaging bag is beneficial to for the second time heat seal sealing apart from sealing 8-10cm.
During use, before the blood drawing, in super-clean bench, cut off outer packaging bag along sealing, take out blood bag body, turn on first the screw lid of a short tube, the anticoagulant heparin agent in the short tube rubber seal injects vacuum blood bag body, is covered screw lid, then in super-clean bench, the outer package of blood bag put again heat seal sealing, and put into 4 ℃ of lower preservations.
During blood drawing, tear outer packaging bag, take out a long tube, take off long tube syringe needle pin medicated cap, open fluid-flow rate control valve valve, utilize vacuum and action of gravitation the blood drawing, get blood complete after, close fluid-flow rate control valve valve, on the pin cap sleeve back stitching head.
After the blood drawing, the blood bag of getting blood is put into the laboratory super-clean bench, turn on another short tube screw lid, to inject dilute blood in the blood bag body with phosphate buffer (PBS) or the culture medium of volume with syringe through the short tube rubber seal with blood volume, then tighten the short tube screw lid, with the liquid blending in the blood bag, the circular hole that dilute blood blood bag body top will be housed again hangs on the hook at super-clean bench top, take off another root long tube syringe needle pin medicated cap, regulate the fluid-flow rate control valve, blood after slowly will diluting oozes from the syringe needle mouth through long tube in blood bag body, and drip to Ficoll is housed centrifuge tube in the Ficoll fluid level on.After every pipe centrifuge tube adds dilute blood, continue the process that density gradient centrifugation is separated mononuclearcell.
This utility model uses easy and simple to handle, and capacity is large, and infection potential is low, and the use procedure consumptive material is few, saves the operating time.Blood bag body top is communicated with two with the short tube of rubber seal and screw lid, need not again heat seal sealing after injecting medicine or solution, and avoids reusing same short tube and risk that infection is arranged; Blood bag body below is communicated with two all with the long tube of syringe needle, avoid drawing blood and discharge the normal a small amount of blood coagulation that occurs of infection and syringe needle place that the blood after the dilution uses same long tube to bring and cause the not smooth problem of circulation, also need not the blood drawing long tube is cut off after with iodine disinfection, reduce operation easier, save the operating time; Long tube is provided with the fluid-flow rate control valve, can control blood flow rate, can emit blood after the dilution with speed very slowly, is beneficial to keep between Ficoll and dilute blood clearly fluid level, and this point is the prerequisite of follow-up density gradient centrifugation success; Syringe needle is provided with the pin medicated cap that can repeat to cover tightly, the pollution in avoid drawing blood operation and the blood bag transportation; Be provided with the outer bag than the long 28cm-32cm of blood bag body, vacant part is used for putting pipe and again heat seal sealing, can avoid the pollution in the blood transfer operating process.
Description of drawings
Fig. 1 is this utility model one example structure sketch map.
Description of drawings
The utility model is described in further detail below in conjunction with accompanying drawing and present embodiment.
With reference to accompanying drawing 1, present embodiment comprises blood bag body 3, described blood bag body 3 tops are provided with one for the circular hole 2 that hangs, described blood bag body 3 volumes are 300ml, described blood bag body 3 tops are connected with two all with the short tube of rubber seal and screw lid, described blood bag body 3 bottoms are connected with two all with the long tube of syringe needle, described long tube is provided with the fluid-flow rate control valve, described syringe needle is provided with the pin medicated cap that can repeat to cover tightly, also comprise the band outer packaging bag, described outer packaging bag is than the long 30cm of blood bag body.
Described short tube length is 2cm.
Described long tube length is 1.2m.
During use, before the blood drawing, in super-clean bench, cut off outer packaging bag along sealing, take out blood bag body 3, turn on first the screw lid of a short tube I 11, the anticoagulant heparin agent in short tube I 11 rubber seals inject vacuum blood bag body 3, is built screw lid, then in super-clean bench, the outer package of blood bag put again heat seal sealing, and put into 4 ℃ of lower preservations.
During blood drawing, tear outer packaging bag, take out long tube I 41, take off long tube I 41 syringe needle I 61 pin medicated caps, open fluid-flow rate control valve I 51 valves, utilize the blood drawing of vacuum and action of gravitation, get blood complete after, close fluid-flow rate control valve I 51 valves, on the pin cap sleeve back stitching I 61.
After the blood drawing, the blood bag of getting blood is put into the laboratory super-clean bench, turn on another short tube II 12 screw lids, to inject blood bag body 3 interior dilute bloods through short tube II 12 rubber seals with syringe with the culture medium of volume with blood volume, then tighten short tube II 12 screw lids, with the liquid blending in the blood bag, the circular hole 2 that dilute blood blood bag body 3 tops will be housed again hangs on the hook at super-clean bench top, take off another root long tube II 42 syringe needle II 62 pin medicated caps, regulate fluid-flow rate control valve II 52, blood after slowly will diluting oozes from the syringe needle mouth through long tube II 42 from blood bag body 3 is interior, and drips on the Ficoll fluid level in the centrifuge tube that Ficoll is housed.After every pipe centrifuge tube adds dilute blood, continue the process that density gradient centrifugation is separated mononuclearcell.
Claims (8)
1. one kind is used for the blood bag that density gradient centrifugation is separated mononuclearcell, comprise blood bag body, described blood bag body top is provided with one for the circular hole that hangs, it is characterized in that, described blood bag volume is 280ml-320ml, and described blood bag body top is connected with two all with the short tube of rubber seal and screw lid, and described blood bag body bottom is connected with two all with the long tube of syringe needle, described long tube is provided with the fluid-flow rate control valve, and described syringe needle is provided with the pin medicated cap that can repeat to cover tightly; Also be provided with outer packaging bag, described outer packaging bag is than the long 28cm-32cm of blood bag body; Outer packaging bag is provided with tearing port.
2. the blood bag for density gradient centrifugation separation mononuclearcell according to claim 1 is characterized in that described this body bulk of blood bag is 300ml.
3. the blood bag for density gradient centrifugation separation mononuclearcell according to claim 1 and 2 is characterized in that described short tube length is 1.5-2.5cm.
4. the blood bag for density gradient centrifugation separation mononuclearcell according to claim 3 is characterized in that described short tube length is 2.0cm.
5. the blood bag for density gradient centrifugation separation mononuclearcell according to claim 1 and 2 is characterized in that described long tube length is 1.0-1.5m.
6. the blood bag for density gradient centrifugation separation mononuclearcell according to claim 5 is characterized in that described long tube length is 1.2m.
7. the blood bag for density gradient centrifugation separation mononuclearcell according to claim 1 and 2 is characterized in that the described outer packaging bag length longer than blood bag body is 30cm.
8. the blood bag for density gradient centrifugation separation mononuclearcell according to claim 1 and 2 is characterized in that, the tearing port on the outer packaging bag is apart from sealing 8-10cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220582266 CN202892429U (en) | 2012-11-07 | 2012-11-07 | Blood bag for density gradient centrifugation of mononuclear cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220582266 CN202892429U (en) | 2012-11-07 | 2012-11-07 | Blood bag for density gradient centrifugation of mononuclear cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202892429U true CN202892429U (en) | 2013-04-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220582266 Expired - Fee Related CN202892429U (en) | 2012-11-07 | 2012-11-07 | Blood bag for density gradient centrifugation of mononuclear cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202892429U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104434139A (en) * | 2014-12-19 | 2015-03-25 | 诸葛宝忠 | Multifunctional intelligent blood sampling system for testing department |
| CN107603880A (en) * | 2017-11-01 | 2018-01-19 | 深圳市职业病防治院 | A kind of cell culture container |
-
2012
- 2012-11-07 CN CN 201220582266 patent/CN202892429U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104434139A (en) * | 2014-12-19 | 2015-03-25 | 诸葛宝忠 | Multifunctional intelligent blood sampling system for testing department |
| CN107603880A (en) * | 2017-11-01 | 2018-01-19 | 深圳市职业病防治院 | A kind of cell culture container |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130424 Termination date: 20131107 |