CN202530084U - Centrifugal tube used for cell inoculation - Google Patents
Centrifugal tube used for cell inoculation Download PDFInfo
- Publication number
- CN202530084U CN202530084U CN 201220182257 CN201220182257U CN202530084U CN 202530084 U CN202530084 U CN 202530084U CN 201220182257 CN201220182257 CN 201220182257 CN 201220182257 U CN201220182257 U CN 201220182257U CN 202530084 U CN202530084 U CN 202530084U
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- inoculation
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- centrifugal tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The utility model relates to a centrifugal tube used for cell inoculation. The centrifugal tube disclosed by the utility model comprises a cylindrical tube body, a top cap and a bottom cap, wherein the cylindrical tube body is provided with a bottom the diameter of which is gradually reduced, the bottom is provided with a fine cylindrical bottom tube; the top cap is connected at the top of the tube body in an opening way; and the bottom cap is detachably sheathed on the bottom tube and is provided with a space used for containing a support for inoculated cells. The centrifugal tube used for cell inoculation disclosed by the utility model is novel in design, the inoculation operation is easy, the requirement for inoculation equipment is lower, the centrifugal tube used for cell inoculation is easy to popularize and apply, and the cell inoculation efficiency is higher, so that cell availability is improved; and the production cost is low, and the centrifugal tube can be made into different specifications as required to meet the actual research requirement, so that the centrifugal tube disclosed by the utility model has a better application prospect in the related research field.
Description
Technical field
The utility model relates to a kind of centrifuge tube that is used for cell inoculation, is mainly used in the Tissue Engineering Study field.
Background technology
The research of seed cell inoculation aspect is still one of the important content in Tissue Engineering Study field.At present, the inoculation method of seed cell relates to the several different methods such as dynamic perfusion and centrifugal inoculation of static inoculation method, bio-reactor.The inoculation required time of static inoculation method is more of a specified duration, and seed cell often can only be seeded in the surface of three-dimensional rack, and the seed cell difficulty is inoculated into the inside of tissue engineering bracket and influences experiment effect.The efficient of the static inoculation of the application of bio-reactor and cell are distributed with bigger improvement in support; But inoculation efficient is still lower; And the design of bio-reactor and schedule of operation relative complex; Expensive, these have limited the popularization and application of its organizational project Related Research Domain to a certain extent.Centrifugal inoculation method makes cell inoculation efficient obtain bigger raising, has also improved the distribution of seed cell in timbering material simultaneously, but has still lacked a kind of centrifuge tube and a kind of generally acknowledged centrifugal inoculation method that is specifically designed to cell inoculation.
For centrifugal inoculation method, also there are many deficiencies when using traditional centrifuge tube to carry out inoculating cell at present.For example, three-dimensional rack is inserted centrifuge tube or when therefrom taking out, can be had some inconveniences; Can increase the chance of cell contamination in the operating process; In addition, the time also have problems, because material is when light for the inoculation that is seeded in of density materials with smaller; Tend to swim in the nutrient solution of centrifuge tube, this also can reduce the inoculation efficient of cell greatly.
Summary of the invention
Use common centrifuge tube some shortcomings on existing cell centrifugation inoculation technique, the utility model to provide a kind of simple in structurely, convenient to be prone to execute and to inoculate the higher centrifuge tube that is used for cell inoculation of efficient in order to overcome.
According to the described a kind of centrifuge tube that is used for cell inoculation of the utility model, said centrifuge tube comprises: cylindrical tube, have the bottom of diameter reduction gradually, and this bottom is provided with thin columniform bottom tube; The top cap can be connected the top of said body with opening; And end cap, removably be socketed on the described bottom tube, and have the space of the support that holds inoculating cell.
The described centrifuge tube that is used for cell inoculation of the utility model has following characteristics and advantage:
(1) improves the bottom design of centrifuge tube, be provided with thin columniform bottom tube in the bottom.
(2) end cap that can be socketed on the described bottom tube is provided, can the support of ready inoculating cell have been inserted in the end cap, again should end cap sleeve have gone into the periphery of bottom tube.
In sum, the described centrifuge tube modern design that is used for cell inoculation of the utility model is inoculated easy and simple to handle; Lower to the inoculation equipment requirements, be easy to popularization and application, and the inoculation efficient of cell is higher; Improved the availability of cell; And it is low to manufacture cost, can be made into the needs that different specifications adapts to practical study as required, thereby can have application promise in clinical practice in the Related Research Domain of organizational project.
Description of drawings
Fig. 1 is the described vertical section synoptic diagram that is used for the centrifuge tube of cell inoculation of the utility model.
Fig. 2 is the synoptic diagram of centrifuge tube under end cap and bottom tube separate stage shown in Figure 1.
Among the figure: 1, top cap; 2, the bottom of body; 3, body; 4, bottom tube; 5, end cap.
Embodiment
Be purpose, structure, characteristic and the function of further understanding the utility model, the detailed description that combines embodiment below is provided.
The described a kind of centrifuge tube that is used for cell inoculation of the utility model, as shown in Figure 1, this centrifuge tube comprises: cylindrical tube 3, have the bottom 2 of diameter reduction gradually, this bottom is provided with thin columniform bottom tube 4; Top cap 1 can be connected the top (in Fig. 1, this top cap 1 is to be in the state of opening, and can carry out application of sample) of body 3 with opening; And end cap 5, removably be socketed on the bottom tube 4, and have the space of the support that holds inoculating cell.
In the operation of centrifugal inoculation method; At first before inoculating cell, be ready to cell suspension and support; Get lower bottom cap, ready support is carefully inserted the periphery of being inserted in the end mouth of body again behind the end cap, open the pipe lid then and ready cell suspension is added blanked-off pipe lid in back in the body.It is centrifugal to select suitable cf-and centrifugation time to carry out according to the concrete cell type of inoculation, generally with 1000 rev/mins of rotating speeds, and the centrifugal seeded process that can accomplish cell in three to five minutes.The cell of counting through blood cell counting plate in the cell suspension of centrifugal front and back can clearly be inoculated in the cell quantity in the support.
Claims (1)
1. a centrifuge tube that is used for cell inoculation is characterized in that, said centrifuge tube comprises:
Cylindrical tube has the bottom of diameter reduction gradually, and this bottom is provided with thin columniform bottom tube;
The top cap can be connected the top of said body with opening;
End cap removably is socketed on the described bottom tube, and has the space of the support that holds inoculating cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220182257 CN202530084U (en) | 2012-04-26 | 2012-04-26 | Centrifugal tube used for cell inoculation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220182257 CN202530084U (en) | 2012-04-26 | 2012-04-26 | Centrifugal tube used for cell inoculation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202530084U true CN202530084U (en) | 2012-11-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220182257 Expired - Fee Related CN202530084U (en) | 2012-04-26 | 2012-04-26 | Centrifugal tube used for cell inoculation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202530084U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104711187A (en) * | 2015-03-25 | 2015-06-17 | 新奥科技发展有限公司 | Cell inoculation device and inoculation method using same |
| GB2574046A (en) * | 2018-05-24 | 2019-11-27 | Oxford Nanopore Tech Ltd | Improved container |
-
2012
- 2012-04-26 CN CN 201220182257 patent/CN202530084U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104711187A (en) * | 2015-03-25 | 2015-06-17 | 新奥科技发展有限公司 | Cell inoculation device and inoculation method using same |
| GB2574046A (en) * | 2018-05-24 | 2019-11-27 | Oxford Nanopore Tech Ltd | Improved container |
| GB2574046B (en) * | 2018-05-24 | 2022-12-07 | Oxford Nanopore Tech Plc | Improved Container |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121114 Termination date: 20150426 |
|
| EXPY | Termination of patent right or utility model |