CN205907251U - Protoplast heavily hangs device - Google Patents
Protoplast heavily hangs device Download PDFInfo
- Publication number
- CN205907251U CN205907251U CN201620815553.7U CN201620815553U CN205907251U CN 205907251 U CN205907251 U CN 205907251U CN 201620815553 U CN201620815553 U CN 201620815553U CN 205907251 U CN205907251 U CN 205907251U
- Authority
- CN
- China
- Prior art keywords
- base
- protoplast
- control panel
- placing box
- groove
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Centrifugal Separators (AREA)
Abstract
The utility model discloses a protoplast heavily hangs device, include the shaking table main part and place the case, be equipped with in the shaking table main part and rotate motor and control panel, the last switch that is equipped with of control panel, the top of placing the case is equipped with the case lid, case lid and place and pass through hinge connection between the case, it is equipped with clamping grooves of a base to place the incasement, clamping grooves of a base is connected through the pivot between the motor with rotating, be equipped with the base among the clamping grooves of a base, the base can block among the clamping grooves of a base just, be equipped with centrifugal tube hole in the base, the inner wall of centrifugation tube hole is 15 to 70 with the size of the contained angle of base horizontal plane. The utility model discloses simple structure, convenient operation, safe in utilization, the effect is reliable, has solved the technical problem that manual heavy outstanding protoplast process wastes time and energy, just can't carry out the high flux operation effectively.
Description
Technical field
This utility model belongs to molecular biology experiment instrument field and in particular to a kind of resuspended device of protoplast.
Background technology
The cell sloughing cell wall, protoplast, is a bionic concept.
The feature of protoplast has: acellular obstacle, can absorb the allogenic materials such as dna Plasma viral bacterial cell device, be
Cell engineering carries out the good material of genetic manipulation gene transfer；There is totipotency regenerable cell wall and regenerate completely individual
Body；It is appropriate to merge and forms hybrid cell, intercellular not affine obstacle not of the same race can be overcome, cultivate new varieties.
Due to there is no cell wall, protoplast be only crop genetic improvement and botany research provide extremely advantageous
Test material, is also widely used in microbe research field.Protoplast can be used for following several research: as cell hybridization
Serve crop or strain improvement；Object as genetic transformation；The generating process of research cell wall；Screening mutant；Film
The research of structure, transport, hormone acceptor site etc.；For separating organelle and macromole；Germ-plasma resources protection.
The most basic principle of protoplast electrofusion is to ensure that protoplast preserves from and do not damage its regeneration capacity, main
The separation method wanted has Mechanical Method separation to separate with enzyme process.After enzymolysis processing, the mixture that obtains be one by protoplast,
Impurity and enzyme liquid are only removed, make protoplast purification, just can be trained by cell mass and the mixed liquor of the compositions such as cell debriss
Support.
Need repeatedly low-speed centrifugal in the purge process of protoplast, remove supernatant, add cpw liquid to carry out resuspended,
Carry out resuspended because protoplast should not acutely shake, prior art generally uses micropipettor gently pressure-vaccum, or hand-held
Centrifuge tube gently rocks and is allowed to hang.Resuspension process is wasted time and energy very much, affects experiment progress, and cannot be carried out high flux
Operation.
Utility model content
For the deficiencies in the prior art, this utility model provides a kind of resuspended device of protoplast, solves protoplast
Resuspension process is wasted time and energy and be cannot be carried out the technical problem of high flux operation.
A kind of resuspended device of protoplast, including shaking table main body and placing box, described shaking table main body is provided with rotary electric machine
And control panel, described control panel is provided with and off switch；The top of described placing box is provided with case lid, described case lid and placement
Pass through gemel connection between case, in described placing box, be provided with base draw-in groove, pass through between described base draw-in groove and rotary electric machine to turn
Axle connect, be provided with base in described base draw-in groove, described base can snap in base draw-in groove just, be provided with described base from
Heart pore, the inwall of described centrifugation pore is 15 ° to 70 ° with the size of the angle of base horizontal plane.
On described base centrifugation pore have 8 kinds of different selections, respectively with 5ml, 7ml, 10ml, 15ml, 20ml,
30ml, 40ml and 50ml centrifuge tube matches.
The described inwall of centrifugation pore and the size of the angle of base horizontal plane are 30 °.
The outer surface of described case lid is provided with handle.
Rotating speed control knob and speed display are additionally provided with described control panel, the rotating speed of described rotary electric machine can lead to
The rotating speed control knob crossed on control panel is adjusted.
Temperature control burtons and temperature indicator are additionally provided with described control panel, the temperature in described placing box can be led to
The temperature control burtons crossed on control panel are adjusted.
Advantage of the present utility model: be provided with multiple centrifugation pores in base of the present utility model, can simultaneously by multiple from
The protoplast pellet of heart pipe carries out resuspended, need not each centrifuge tube be operated successively again, saved substantial amounts of manpower and when
Between；Base of the present utility model can be changed, the different centrifugation pore of every kind of base correspondence, can meet in experiment using difference
The demand of the centrifuge tube of size；Described centrifuge tube hole wall and base horizontal plane are provided with the angle of certain angle, can make protoplasm
It is resuspended that body is more prone to；Rotating speed of the present utility model and temperature all can be adjusted, and can meet in experiment to not
Needs with rotating speed and temperature.Summary, this utility model structure is simple, easy to operate, and using safety, effect is reliable, has
Solve the technical problem that resuspended protoplast process manually wastes time and energy and cannot be carried out high flux operation to effect.
Brief description
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of base of the present utility model；
Reference: 1, shaking table main body；2nd, placing box；3rd, rotary electric machine；4th, control panel；5th, on and off switch；6th, case lid；
7th, hinge；8th, base draw-in groove；9th, rotating shaft；10th, base；11st, it is centrifuged pore；12nd, inwall；13rd, base horizontal plane；14th, angle；
15th, handle；16th, rotating speed control knob；17th, speed display；18th, temperature control burtons；19th, temperature indicator.
Specific embodiment
With reference to the accompanying drawings and detailed description this utility model is described in further detail.
Embodiment 1
As shown in Figure 1-2, the resuspended device of a kind of protoplast, including shaking table main body 1 and placing box 2, described shaking table main body 1
It is provided with rotary electric machine 3 and control panel 4, described control panel 4 is provided with and off switch 5；The top of described placing box 2 is provided with
Case lid 6, is connected by hinge 7 between described case lid 6 and placing box 2, is provided with base draw-in groove 8, described base in described placing box 2
It is connected by rotating shaft 9 between draw-in groove 8 and rotary electric machine 3, be provided with base 10 in described base draw-in groove 8, described base 10 can be proper
Snap in well in base draw-in groove 8, in described base 10, be provided with centrifugation pore 11, the inwall 12 of described centrifugation pore 11 and base level
The size of the angle 14 in face 13 is 15 °.
Embodiment 2
As different from Example 1, on described base 10 centrifugation pore 11 have 8 kinds of different selections, respectively with 5ml,
7ml, 10ml, 15ml, 20ml, 30ml, 40ml and 50ml centrifuge tube matches.
Embodiment 3
As different from Example 1, the size of the angle 14 of the inwall 12 of described centrifugation pore 11 and base horizontal plane 13
For 30 °.
Embodiment 4
As different from Example 1, the size of the angle 14 of the inwall 12 of described centrifugation pore 11 and base horizontal plane 13
For 70 °.
Embodiment 5
As different from Example 1, described control panel 4 is additionally provided with rotating speed control knob 16 and speed display 17,
The rotating speed of described rotary electric machine 3 can be adjusted by the rotating speed control knob 16 on control panel 4.
Embodiment 6
As different from Example 1, described control panel 4 is additionally provided with temperature control burtons 18 and temperature indicator 19,
Temperature in described placing box 2 can be adjusted by the temperature control burtons 18 on control panel 4.
Although, above with general explanation and specific embodiment, detailed description is made to this utility model,
On the basis of this utility model, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements on the basis of without departing from this utility model spirit, belonging to this utility model will
Seek the scope of protection.
Claims (6)
1. a kind of resuspended device of protoplast it is characterised in that: include shaking table main body and placing box, described shaking table main body is provided with
Rotary electric machine and control panel, described control panel is provided with and off switch；The top of described placing box is provided with case lid, described case
Pass through gemel connection between lid and placing box, in described placing box, be provided with base draw-in groove, described base draw-in groove and rotary electric machine it
Between connected by rotating shaft, be provided with base in described base draw-in groove, described base can snap in base draw-in groove just, described base
In be provided with centrifugation pore, the inwall of described centrifugation pore is 15 ° to 70 ° with the size of the angle of base horizontal plane.
2. the resuspended device of protoplast according to claim 1 it is characterised in that: the centrifugation pore on described base has 8
Plant different selections, match with 5ml, 7ml, 10ml, 15ml, 20ml, 30ml, 40ml and 50ml centrifuge tube respectively.
3. the resuspended device of protoplast according to claim 2 it is characterised in that: the inwall of described centrifugation pore and base
The size of the angle of horizontal plane is 30 °.
4. the resuspended device of protoplast according to claim 3 it is characterised in that: the outer surface of described case lid is provided with handle
Handss.
5. the resuspended device of the protoplast according to any one of claim 1-4 it is characterised in that: on described control panel also
It is provided with rotating speed control knob and speed display, the rotating speed of described rotary electric machine can be controlled by the rotating speed on control panel to be pressed
Button is adjusted.
6. the resuspended device of protoplast according to claim 5 it is characterised in that: be additionally provided with temperature on described control panel
Control knob and temperature indicator, the temperature in described placing box can be adjusted by the temperature control burtons on control panel
Section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620815553.7U CN205907251U (en) | 2016-07-29 | 2016-07-29 | Protoplast heavily hangs device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620815553.7U CN205907251U (en) | 2016-07-29 | 2016-07-29 | Protoplast heavily hangs device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205907251U true CN205907251U (en) | 2017-01-25 |
Family
ID=57803729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620815553.7U Expired - Fee Related CN205907251U (en) | 2016-07-29 | 2016-07-29 | Protoplast heavily hangs device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205907251U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934316A (en) * | 2018-09-12 | 2018-12-07 | 山西省农业科学院棉花研究所 | A kind of equidistant monoseeding device of wheat |
-
2016
- 2016-07-29 CN CN201620815553.7U patent/CN205907251U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934316A (en) * | 2018-09-12 | 2018-12-07 | 山西省农业科学院棉花研究所 | A kind of equidistant monoseeding device of wheat |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204714819U (en) | A kind of Tissue Culture Flask of belt supporting frame | |
| CN205907251U (en) | Protoplast heavily hangs device | |
| CN204185484U (en) | A kind of rapid extraction device for nucleic acid | |
| CN210994803U (en) | Stem cell centrifugal device | |
| CN203174084U (en) | Magnetic needle and casing mobile system for magnetic bar method extractor | |
| CN111172014A (en) | Stem cell culture solution preparation device and stem cell culture solution preparation method | |
| CN107022479A (en) | A kind of bacterial reproduction case for carrying out sample comparison | |
| CN206549566U (en) | A kind of raw material agitator processed for silica gel | |
| CN207227452U (en) | A kind of centrifugation cell incubator | |
| CN206587804U (en) | A kind of centrifuge tube shelf | |
| CN201273873Y (en) | Liquid base shallow layer cell tabletting apparatus | |
| CN212393366U (en) | Matrix material is equipped with device | |
| CN207928778U (en) | A kind of adhesive evacuation and centrifugal degassing device | |
| CN207076567U (en) | A kind of blood bag fixing device for blood separation | |
| CN103923162A (en) | Vibration mechanism for magnetic rod technique bioactive substance extractor and application thereof | |
| CN206901032U (en) | A kind of stemming raw materials for production material storage device | |
| CN205731340U (en) | A kind of test tube rack prepared for agar slant culture-medium | |
| CN203681612U (en) | Transfer trolley for preparing blood components | |
| CN214150102U (en) | Compound fertilizer detection is with dividing appearance device | |
| CN210815693U (en) | A small amount of medicinal materials magnetic separation auxiliary device | |
| CN206143105U (en) | Be suitable for protein purification's high carrying capacity antibody purification device | |
| CN206229646U (en) | A kind of diamond sorting unit | |
| CN214864428U (en) | Cell separation and extraction equipment | |
| CN204543740U (en) | A kind of novel algae liquid concentrating and separating device | |
| CN204198735U (en) | The save set of absorbent filter in a kind of RNA leaching process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170125 Termination date: 20180729 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |