CN205170865U - Slice cell culture carrier and cell culture bottle - Google Patents
Slice cell culture carrier and cell culture bottle Download PDFInfo
- Publication number
- CN205170865U CN205170865U CN201520946046.2U CN201520946046U CN205170865U CN 205170865 U CN205170865 U CN 205170865U CN 201520946046 U CN201520946046 U CN 201520946046U CN 205170865 U CN205170865 U CN 205170865U
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- China
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- cell culture
- bottle
- carrier
- sheet cell
- utility
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model provides a slice cell culture carrier and cell culture bottle for adherence dependence cell culture. This is cultivateed the carrier shape and is the elongate, and the length -width ratio is 3 -80. Cell culture bottle includes bottle and bottle lid, is provided with slice cell culture carrier in the bottle. Adopt the utility model discloses an adherence dependent form cell is trained to slice cell culture carrier and blake bottle, and it is good to train carrier suspension in cell culture liquid, rare glue glutinous to greatly improve and adhere to the area, it is especially adapted in high density cell culture.
Description
Technical field
The utility model relates to a kind of sheet cell culture vector and Tissue Culture Flask, for carrying out anchorage-dependent cell high-density culture.
Background technology
At present, known sheet cell culture vector has multiple.These chip carriers or complex structure, manufacture difficulty is large, and production cost is high, is difficult to apply.Area is large, sticks mutually in cell culture container, significantly reduces cell attachment area, is unfavorable for the high-density culture of cell.
Utility model content
The purpose of this utility model is to provide a kind of sheet cell culture vector and Tissue Culture Flask, and structure is simple, reasonable in design, easy to use, for the high-density culture of anchorage-dependent cell.
According to first aspect of the present utility model, provide a kind of sheet cell culture vector, support shapes is elongated shape, and long-width ratio is 3-80.
Under preferable case, length is 1 centimetre-4 centimetres, and wide is 0.05 centimetre-0.3 centimetre.
Under preferable case, carrier grammes per square metre is 80 grams/m-180 grams/m.
Under preferable case, carrier is made up of one or more layers non-woven fabrics.
Under preferable case, non-woven fabrics is made up of pet fiber.
Under preferable case, described fiber fineness is the 1-10 dawn.
According to second aspect of the present utility model, provide a kind of Tissue Culture Flask, comprise bottle and bottle cap, in bottle, be provided with above-mentioned sheet cell culture vector.
Under preferable case, wherein bottle cap is provided with liquid-inlet pipe, liquid discharge pipe and ventpipe.
Under preferable case, in ventpipe, be provided with filter core.
Under preferable case, liquid-inlet pipe end is provided with skimmer.
The beneficial effects of the utility model are, structure is simple, and production difficulty is little, is conducive to spread.Carrier is easy to use, and surface-area is little, can not be adhered to one another in cell culture container, is beneficial to the high-density culture of cell.
Accompanying drawing explanation
Fig. 1 is according to a kind of sheet cell culture vector schematic diagram of the present utility model.
Fig. 2 is according to a kind of Tissue Culture Flask cross-sectional schematic of the present utility model.
Embodiment
Below in conjunction with drawings and Examples, the utility model is further illustrated.It will be appreciated by those skilled in the art that embodiment described below is only to exemplary illustration of the present utility model, but not for making any restriction to it.
Fig. 1 shows according to a kind of sheet cell culture vector of the present utility model, for carrying out the cultivation of anchorage-dependent cell.The shape of this sheet cell culture vector is elongated shape, and long-width ratio is 3-80, is preferably 10-50.In some specific embodiment, carrier length is 1 centimetre-4 centimetres, and wide is 0.05 centimetre-0.3 centimetre, and grammes per square metre is 80 grams/m-180 grams/m.
Cell culture vector can be made up of one or more layers polyethylene terephthalate (PET) non-woven fabrics, and this non-woven fabrics is the known current material of this area.In preferred embodiment of the present utility model, the fineness of PET being used for being formed non-woven fabrics is 1-10 dawn (in the art, 9000 meters of long stapled grammes per square metre numbers are called the dawn, in order to represent fiber fineness).Adopt the cell culture vector made by fiber of this fineness scope, its void density is higher, and surface-area is large, is beneficial to attaching and the cultivation of cell.
Fig. 2 shows according to a kind of Tissue Culture Flask of the present utility model, comprises bottle 1 and bottle cap 2, and bottle cap 2 and bottle 1 can be screwed by screw thread.Above-mentioned sheet cell culture vector 3 is provided with in bottle 1.Wherein, the loading capacity of culture carrier 3 is preferably 10-50g/L, and culture carrier 3 is present in Tissue Culture Flask with natural shape, curling shape or accordion.
Bottle cap 2 is provided with liquid-inlet pipe 4, liquid discharge pipe 6 and ventpipe 5.Liquid-inlet pipe 4 is used for introducing cell culture fluid in Tissue Culture Flask, and liquid discharge pipe 6 is used for outwards drawing cell culture fluid.Liquid-inlet pipe 4 can be connected peristaltic pump respectively with liquid discharge pipe 6, thus can to set flow and speed carries out topping up and discharge opeing to culturing bottle.Ventpipe 5 is used for keeping the air (or oxygen) inside and outside bottle to exchange.In order to prevent the pollutent such as bacterium in air from entering culturing bottle, can be provided with filter core 11 in ventpipe 5, filter core preferably adopts 0.22 micron air filter core.
In certain embodiments, liquid-inlet pipe 4 is connected flexible rubber hose 7 and 10 respectively with the lower end of liquid discharge pipe 6, is extended to the bottom of culturing bottle by flexible rubber hose 7 and 10.In a preferred embodiment of the present utility model, the end (i.e. the end of flexible rubber hose 7) of liquid-inlet pipe 4 is provided with skimmer 8.Skimmer 8 can enter into culturing bottle by being dispersed into multiply thread from one nutrient solution in liquid-inlet pipe 4, thus effectively prevents the impact when adding nutrient solution to cell culture vector 3.
The working process of the utility model sheet cell culture vector and Tissue Culture Flask is briefly described below.
Load in culturing bottle by cell culture vector 3, after sterilising treatment, add treat cultured cells suspension to Tissue Culture Flask, cell density is 1,000,000/milliliter.Fill up cell culture fluid, carry out Cell infusion cultivation.Cell culture fluid is regularly replaced by liquid-inlet pipe 4 and liquid discharge pipe 6.Until cell density reaches more than 1,000 ten thousand/milliliters, complete cell cultures.
Adopt sheet cell culture vector of the present utility model and culturing bottle to cultivate anchorage-dependent cells, culture carrier suspension in cell culture fluid is good, seldom occurs sticky glutinous, thus greatly improves bond area, be very suitable for concentration cultivation.
Be only preferred embodiment of the present utility model above, all equalizations done according to the utility model claim change or modify, and all should belong to the covering scope of the utility model claim.
Claims (10)
1. a sheet cell culture vector, is characterized in that, this culture carrier shape is elongated shape, and long-width ratio is 3-80.
2. sheet cell culture vector according to claim 1, is characterized in that, length is 1 centimetre-4 centimetres, and wide is 0.05 centimetre-0.3 centimetre.
3. sheet cell culture vector according to claim 1, is characterized in that, carrier grammes per square metre is 80 grams/m-180 grams/m.
4. sheet cell culture vector according to claim 1, is characterized in that, carrier is made up of one or more layers non-woven fabrics.
5. sheet cell culture vector according to claim 4, it is characterized in that, non-woven fabrics is made up of pet fiber.
6. sheet cell culture vector according to claim 5, is characterized in that, described fiber fineness is the 1-10 dawn.
7. a Tissue Culture Flask, comprises bottle and bottle cap, it is characterized in that, be provided with in bottle as arbitrary in claim 1-6 as described in sheet cell culture vector.
8. Tissue Culture Flask according to claim 7, is characterized in that, wherein bottle cap is provided with liquid-inlet pipe, liquid discharge pipe and ventpipe.
9. Tissue Culture Flask according to claim 8, is characterized in that, is provided with filter core in ventpipe.
10. Tissue Culture Flask according to claim 8, is characterized in that, liquid-inlet pipe end is provided with skimmer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520946046.2U CN205170865U (en) | 2015-11-24 | 2015-11-24 | Slice cell culture carrier and cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520946046.2U CN205170865U (en) | 2015-11-24 | 2015-11-24 | Slice cell culture carrier and cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205170865U true CN205170865U (en) | 2016-04-20 |
Family
ID=55734879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520946046.2U Expired - Fee Related CN205170865U (en) | 2015-11-24 | 2015-11-24 | Slice cell culture carrier and cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205170865U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114790423A (en) * | 2021-01-26 | 2022-07-26 | 罗益（无锡）生物制药有限公司 | Cell culture device for hemorrhagic fever vaccine |
-
2015
- 2015-11-24 CN CN201520946046.2U patent/CN205170865U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114790423A (en) * | 2021-01-26 | 2022-07-26 | 罗益（无锡）生物制药有限公司 | Cell culture device for hemorrhagic fever vaccine |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160420 Termination date: 20181124 |