CN204918609U

CN204918609U - Cell culture bag that volume changes

Info

Publication number: CN204918609U
Application number: CN201520686959.5U
Authority: CN
Inventors: 颜学恒; 张智培
Original assignee: Shanghai Xp Biomed Ltd
Current assignee: Shanghai Xp Biomed Ltd
Priority date: 2015-09-07
Filing date: 2015-09-07
Publication date: 2015-12-30
Anticipated expiration: 2025-09-07

Abstract

The utility model provides a cell culture bag that volume changes, includes the bag physically, a plurality of connecting portion that the bag was personally experienced sth. Part of the body and is had interval distribution from top to bottom and can tear between two relative lateral walls, this bag of body passes through a plurality of coupling part separate into bag at the bottom of a plurality of expansions bags and, ventilative mouth, sample connection, transfer line interface and inoculation cell interface has on the end bag. The utility model discloses simple structure, preparation are convenient, when culture cell, select to tear one or a plurality of connecting portion according to cell density and can expand the volume, need not change the container, reduce the manual operation flow by a wide margin, do not need operation technique just can let the cell maintain certain density simultaneously, and the cell can not polluted to the continued growth to conveniently, the success rate is high.

Description

The cell culture bags of volume change

Technical field

The utility model relates to cell culture technology, particularly relates to the cell culture bags of a kind of volume change.

Background technology

Cell cultures has now been widely used in the every field such as biology, medical science, new drug development, becomes one of most important basic science.

Traditional cell culture processes, need to maintain certain cell density, therefore culture vessel volume can not be excessive, otherwise cell density is too low, cannot grow; But when cell proliferation is to some amount, exceed certain density, then too intensive cell can stop growing, even aging apoptosis.Now must change the culture vessel of larger volume, need the operative technique of extreme care, to avoid cell contamination, very inconvenient and be easy to failure.

Utility model content

Based on this, for above-mentioned technical problem, provide the cell culture bags that a kind of volume changes.

For solving the problems of the technologies described above, the utility model adopts following technical scheme:

A kind of cell culture bags of volume change, comprise bag, between two sidewalls that described bag is relative, there are spaced apart and peelable multiple connection sections from top to bottom, this bag is divided into bag at the bottom of multiple expansion bag and by described multiple connection portion, bag of the described end has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

Described connection section is made up of two sidewall local gluings.

Described connection section is connected and composed by self-sealed article by two sidewalls, described self-sealed article comprises master bar and public bar, described master bar and public bar are fixed on described two sidewalls relatively, and the outside surface of described master bar has notch, and described public bar has the card division that can snap in described notch.

Bag of the described end is funnel-form.

An expansion bag in described multiple expansion bag has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

In described ventilation mouth, there is filter core.

Described expansion bag has scale marker.

The utility model structure is simple, easy to make, when culturing cell, select to tear one or more connection section and extendible volume according to cell density, container need not be changed, significantly reduce manual operation flow process, the density simultaneously not needing operative technique that cell just can be allowed to remain certain, continued growth, can not contamination of cells, and convenient, success ratio is high.

Accompanying drawing explanation

Below in conjunction with the drawings and specific embodiments, the utility model is described in detail:

Fig. 1 is structural representation of the present utility model;

Fig. 2 is side-looking structural representation of the present utility model;

Fig. 3 is the structural representation of the connection section of the utility model one embodiment;

Fig. 4 is the structural representation of the connection section of another embodiment of the utility model.

Embodiment

As shown in Figure 1 and Figure 2, a kind of cell culture bags of volume change, comprises bag 110.

Have spaced apart and peelable multiple connection sections 111 from top to bottom between two sidewalls that bag 110 is relative, this bag 110 is separated into bag 113 at the bottom of multiple expansion bag 112 and by multiple connection section 111.

When culturing cell, select to tear one or more connection section 111 i.e. extendible volume according to cell density, container need not be changed, significantly reduce manual operation flow process, the density simultaneously not needing operative technique that cell just can be allowed to remain certain, continued growth, can not contamination of cells, and convenient, success ratio is high.

Wherein, end bag 113 has ventilation mouth 114, thief hole 115, tubing interface 116 and inoculating cell interface 117.

Preferably, an expansion bag 112 in multiple expansion bag 112 also has ventilation mouth 114, thief hole 115, tubing interface 116 and inoculating cell interface 117.

In ventilation mouth 114, there is filter core, air free diffusing can be allowed to enter in bag 110, but other materials or bacterium can not be allowed to pass.

Thief hole 115, for sampling quality inspection, is conveniently monitored.

Tubing interface 116 can be connected with tubing, also can inject liquid by syringe.

Inoculating cell interface 117 can inoculating cell.

Wherein, expand on bag 112 and have scale marker, user correctly can know culture volume.

End bag 113, in funnel-form, can allow cell lean on bottom gravity natural subsidence to sack, reduces cell centrifugation amount, convenient operation.

As shown in Figure 3, in one embodiment, connection section 111 is made up of two sidewall local gluings.

As shown in Figure 4, in another embodiment, connection section 111 is connected and composed by self-sealed article 120 by two sidewalls, self-sealed article 120 comprises master bar 121 and public bar 122, master bar 121 and public bar 122 are fixed on two sidewalls relatively, the outside surface of master bar 121 has notch, public bar 122 has the card division 122a that can snap in notch.

But, those of ordinary skill in the art will be appreciated that, above embodiment is only used to the utility model is described, and be not used as restriction of the present utility model, as long as in spirit of the present utility model, all will drop in Claims scope of the present utility model the change of the above embodiment, modification.

Claims

1. the cell culture bags of a volume change, it is characterized in that, comprise bag, between two sidewalls that described bag is relative, there are spaced apart and peelable multiple connection sections from top to bottom, this bag is divided into bag at the bottom of multiple expansion bag and by described multiple connection portion, bag of the described end has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

2. the cell culture bags of a kind of volume change according to claim 1, is characterized in that, described connection section is made up of two sidewall local gluings.

3. the cell culture bags of a kind of volume change according to claim 1, it is characterized in that, described connection section is connected and composed by self-sealed article by two sidewalls, described self-sealed article comprises master bar and public bar, described master bar and public bar are fixed on described two sidewalls relatively, the outside surface of described master bar has notch, and described public bar has the card division that can snap in described notch.

4. the cell culture bags of a kind of volume change according to Claims 2 or 3, it is characterized in that, bag of the described end is funnel-form.

5. the cell culture bags of a kind of volume change according to claim 4, is characterized in that, an expansion bag in described multiple expansion bag has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

6. the cell culture bags of a kind of volume change according to claim 5, is characterized in that having filter core in described ventilation mouth.

7. the cell culture bags of a kind of volume change according to claim 6, is characterized in that described expansion bag has scale marker.