CN202465711U - Multifunctional stem cell culture plate - Google Patents
Multifunctional stem cell culture plate Download PDFInfo
- Publication number
- CN202465711U CN202465711U CN 201220124791 CN201220124791U CN202465711U CN 202465711 U CN202465711 U CN 202465711U CN 201220124791 CN201220124791 CN 201220124791 CN 201220124791 U CN201220124791 U CN 201220124791U CN 202465711 U CN202465711 U CN 202465711U
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- culture plate
- culture
- cell
- stem cell
- plate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
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- Wood Science & Technology (AREA)
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a multifunctional stem cell culture plate, which comprises an upper cover, a culture plate and culture plate holes, wherein the culture plate is provided with the culture plate holes; the bottom surfaces of the culture plate holes are provided with a layer of filter membrane respectively; the aperture of the filter membrane is 0.4 mum; and a liquid suction hole with the diameter of 13-18 mm is formed at the right upper corner of the culture plate and is connected with the bottoms of the culture plate holes through pipelines. The multifunctional stem cell culture plate has a simple structure and is convenient to use; cell thawing experimental procedures can be effectively reduced; cryopreservation liquid can be directly removed without centrifuging cells; a fresh culture medium is added, so that the time of cell thawing operation is greatly shortened; similarly, the old culture medium of the entire plate can be removed at one time by culturing the cells with the plate, so that the liquid changing time during culture is greatly saved, and different straws are not required to be replaced for each hole to save experimental consumables and guarantee safety and reliability.
Description
Technical field
The utility model relates to the Tissue Culture Plate field, is specially the multi-functional culture plate of a kind of stem cell.
Background technology
Stem cell is one type of multipotential cell with self, and under certain condition, it can be divided into multiple functioning cell.Potentiality of development according to stem cell is divided three classes: myeloid-lymphoid stem cell, multipotential stem cell and unipotent stem cell.Stem cell is that the organizational project application provides seed cell and for exploring human effective tool of growing secret, huge prospect arranged all in fundamental research and clinical application.
When culturing stem cells, after cell proliferation reaches certain density, can carry out frozen to it.The timely frozen of cell transports and transmits, guarantees that for cell the stable and cell material of clone can permanent use of quilt have important meaning.Conventional frozen method is frozen at a slow speed, and promptly collecting cell is placed in the refrigerating fulid of 4 ℃ of precoolings, places in the freezing storing box-80 ℃ after about 12 hours, immerses liquid nitrogen and preserves.Frozen at a slow speed getting widely because of it is convenient, simple used.The frozen at a slow speed cell processes of recovery is: after 37 ℃ of water-baths are thawed, change in the fresh culture, re-suspended cell is inoculated in culture plate behind the centrifugal 10min.But the freeze-stored cell cell proportion of in the recovery process, surviving is on the low side at a slow speed, especially stem cell, as: human embryo stem cell, its recovery efficient is lower than 30%.Therefore how improving the frozen at a slow speed recovery efficient of stem cell is distinct issues.The toxicity that wherein to influence important factor of recovery efficient be exactly cells frozen storing liquid; Therefore the prescription of improveing frozen storing liquid is to reduce the toxic approach of cells frozen storing liquid; But the frozen storing liquid after the improvement often is accompanied by the increase and the recovery efficient of cost and does not improve significantly, and also is difficult to satisfy the needs of experiment for some rare cells.
The utility model content
The technical problem that the utility model solved is to provide a kind of stem cell multi-functional culture plate.This culture plate can shorten the time of recovery cell fast, and recovery polyclonal cellular simultaneously, and reduces freeze-stored cell and thaw the time that the back contact with cells frozen storing liquid, and raising recovery efficient is with the problem in the solution above-mentioned background technology.
The technical problem that the utility model solved adopts following technical scheme to realize: the multi-functional culture plate of a kind of stem cell; Comprise: loam cake, culture plate and cultivation plate hole; Establish the cultivation plate hole on the said culture plate, said cultivation plate hole bottom surface is provided with one deck filter membrane, and the filter membrane aperture is 0.4 μ m; The said culture plate upper right corner is provided with the liquid outlet that a diameter is 13 ~ 18mm, and said liquid outlet is cultivated the plate hole bottom through pipe connection, and liquid outlet connects the negative pressure-pumping device through suction pipe, can be easily all be cultivated that the liquid of plate holes is disposable to be siphoned away fast; Said cultivation plate hole bottom is divided into nine zones with superfine nine palace lattice (lines are impression, no color), can make things convenient for cell clone or cell counting.
Beneficial effect:
The utility model is simple in structure, and is easy to use, can effectively reduce the cell recovery experimental procedure, and eccentric visual cell just can directly be removed frozen storing liquid again, adds fresh culture, thereby shortens the time of cell recovery operation greatly; Owing to can design plurality of specifications, like single hole, 4 holes, 6 holes, 12 holes, 24 holes, reach the corresponding cell strain number of recovering simultaneously, be suitable for extensive stem cell recovery.Utilize this plate culturing cell equally, remove old substratum can disposablely for whole plate, practiced thrift the time of changing liquid when cultivating greatly, need not change different suction pipes, practiced thrift the experiment consumptive material for each hole, simultaneously safe and reliable.Because nine palace lattice are arranged at the bottom, make things convenient for pair cell clone or cell to count, the convenient cell recovery efficient of calculating.
Description of drawings
Fig. 1 is the plan structure synoptic diagram of the utility model.
Embodiment
For technique means, creation characteristic that the utility model is realized, reach purpose and be easy to understand understanding with effect, below in conjunction with concrete diagram, further set forth the utility model.
Referring to Fig. 1, the multi-functional culture plate of a kind of stem cell comprises: loam cake 4, culture plate 1 and cultivation plate hole 2, and to establish on the said culture plate 1 and cultivate plate hole 2, said cultivation plate hole 2 bottom surfaces are provided with one deck filter membrane (not shown), and the filter membrane aperture is 0.4 μ m; Said culture plate 1 upper right corner is provided with the liquid outlet 3 that a diameter is 13 ~ 18mm; Said liquid outlet 3 connects each through the pipeline (not shown) and cultivates plate hole 2 bottoms; Liquid outlet 3 connects the negative pressure-pumping device through suction pipe, can be easily all be cultivated that the liquid of plate holes 2 is disposable to be siphoned away fast; Said cultivation plate hole 2 bottoms are divided into nine zones with superfine nine palace lattice (lines are impression, no color), can make things convenient for cell clone or cell counting.
During use, the cell suspension that 37 ℃ of water-baths are thawed adds after each cultivates plate hole 2, and suction pipe is aimed at liquid outlet 3; Be communicated with the negative pressure-pumping device, all liquid of cultivating plate hole 2 just can disposablely siphon away fast, add fresh substratum then; Mixing can place 37 ℃ of cell culture incubators to cultivate gently.
More than show and described the advantage of ultimate principle, principal character and the utility model of the utility model.The technician of the industry should understand; The utility model is not restricted to the described embodiments; The principle of describing in the foregoing description and the specification sheets that the utility model just is described; Under the prerequisite that does not break away from the utility model spirit and scope, the utility model also has various changes and modifications, and these variations and improvement all fall in the utility model scope that requires protection.The utility model requires protection domain to be defined by appending claims and equivalent thereof.
Claims (1)
1. the multi-functional culture plate of stem cell; Comprise: loam cake, culture plate and cultivation plate hole, establish the cultivation plate hole on the said culture plate, it is characterized in that; Said cultivation plate hole bottom surface is provided with one deck filter membrane; The filter membrane aperture is 0.4 μ m, and the said culture plate upper right corner is provided with the liquid outlet that a diameter is 13 ~ 18mm, and said liquid outlet is cultivated the plate hole bottom through pipe connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220124791 CN202465711U (en) | 2012-03-29 | 2012-03-29 | Multifunctional stem cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220124791 CN202465711U (en) | 2012-03-29 | 2012-03-29 | Multifunctional stem cell culture plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202465711U true CN202465711U (en) | 2012-10-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220124791 Expired - Lifetime CN202465711U (en) | 2012-03-29 | 2012-03-29 | Multifunctional stem cell culture plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202465711U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103992949A (en) * | 2014-05-29 | 2014-08-20 | 中国科学院合肥物质科学研究院 | Detection device of nanomaterial-induced deoxyribonucleic acid (DNA) damage and quick detection method |
| CN104232486A (en) * | 2014-09-28 | 2014-12-24 | 浙江大学常州工业技术研究院 | Culture plate for unicellular clone culture and application method of culture plate |
| WO2021218472A1 (en) * | 2020-04-30 | 2021-11-04 | Peng Xingyue | Method for constructing slow microcyclic artificial cell niche and apparatus thereof |
-
2012
- 2012-03-29 CN CN 201220124791 patent/CN202465711U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103992949A (en) * | 2014-05-29 | 2014-08-20 | 中国科学院合肥物质科学研究院 | Detection device of nanomaterial-induced deoxyribonucleic acid (DNA) damage and quick detection method |
| CN104232486A (en) * | 2014-09-28 | 2014-12-24 | 浙江大学常州工业技术研究院 | Culture plate for unicellular clone culture and application method of culture plate |
| CN104232486B (en) * | 2014-09-28 | 2016-08-24 | 浙江大学常州工业技术研究院 | For single cell clone cultivation culture plate and application process thereof |
| WO2021218472A1 (en) * | 2020-04-30 | 2021-11-04 | Peng Xingyue | Method for constructing slow microcyclic artificial cell niche and apparatus thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20121003 |
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| CX01 | Expiry of patent term |