CN203212577U

CN203212577U - Deep-hole cell culture plate with baffle plates

Info

Publication number: CN203212577U
Application number: CN 201220734802
Authority: CN
Inventors: 白仲虎; 聂简琪; 龙泉
Original assignee: Jiangnan University
Current assignee: Jiangnan University
Priority date: 2012-12-28
Filing date: 2012-12-28
Publication date: 2013-09-25
Anticipated expiration: 2022-12-28

Abstract

The utility model provides a deep-hole cell culture plate with baffle plates. The deep-hole cell culture plate is capable of improving the volumetric dissolved oxygen coefficient of the culture plate so that the volumetric dissolved oxygen coefficient achieves the level similar to the volumetric dissolved oxygen coefficient of a fermentation tank. The deep-hole cell culture plate comprises a culture plate, wherein deep culture holes are evenly distributed on the culture plate. The deep-hole cell culture plate is characterized in that four baffle plates are arranged at the inner periphery of each deep culture hole, the four baffle plates are evenly distributed along the peripheral surface, two adjacent baffle plates are staggered in the hole depth direction of the deep culture hole, and two opposite baffle plates are arranged at equal height in parallel in the hole depth direction of the deep culture hole.

Description

A kind of deep hole Tissue Culture Plate with baffle plate

Technical field

The utility model relates to the cell culture apparatus technical field, relates in particular to the high-flux cell of cultivating aerobic cell and cultivates the culture plate of usefulness, is specially a kind of deep hole Tissue Culture Plate with baffle plate.

Background technology

Tissue Culture Plate can utilize less nutrient solution to carry out the cultivation of cell high-throughput and bacterial classification or cell strain screening, have fast, characteristics such as high-throughput, saving cost, in the process of cell cultures, the general Tissue Culture Plate that uses earlier carries out flux height, cell cultures that volume is little, filter out optimum bacterial classification, and tentatively determine preferable technology culture condition (temperature, pH value, substratum etc.).Then bacterial classification and the preliminary processing condition of determining that filter out are applied in the fermentor tank, carry out the scale amplification culture.

In the scale amplification process of cell cultures, fermentor tank mostly is equipped with whipping appts or apparatus of oxygen supply, fermentor tank can provide higher volume dissolved oxygen coefficient in aerobic cell cultivation process, and existing deep hole Tissue Culture Plate is just simply by making the nutrient solution vibration in the culture hole under the external effect that certain rotating speed and eccentricity be provided, by increasing the contact area of nutrient solution and air, improve liquid mixing and carry out oxygen supply, gas-liquid is practised physiognomy little in the culturing process, the liquid mixing effect is relatively poor, make volume dissolved oxygen coefficient that culture plate provides significantly less than fermentor tank, because the remarkable difference of volume dissolved oxygen coefficient, scale based on culture plate is amplified the cultivation results that can not accurately predict in the fermentor tank, and the bacterial classification that uses culture plate to filter out simultaneously might not be best suited for fermentor tank.

Summary of the invention

At the problems referred to above, the utility model provides a kind of deep hole Tissue Culture Plate with baffle plate, and the volume dissolved oxygen coefficient that it can improve culture plate reaches the volume dissolved oxygen coefficient level of approximation with fermentor tank.

Its technical scheme is such, a kind of deep hole Tissue Culture Plate with baffle plate, it comprises culture plate, described culture plate is evenly distributed with the cultivation deep hole, it is characterized in that: described cultivation deep hole be provided with four baffle plates interior week, described four baffle plates are uniform along side face, and adjacent two described baffle plates are arranged two relative described baffle plates contour being arranged in parallel on the hole depth direction of described cultivation deep hole along dislocation on the hole depth direction of described cultivation deep hole.

It is further characterized in that:

Described cultivation deep hole cross section is square, described square square and the rectangle of comprising;

Described baffle plate is arc, is arranged at four jiaos of described cultivation deep holes, is applicable to the cultivation to the cell of shearing force sensitivity;

Described baffle plate is bar shaped, is arranged at described cultivation deep hole hole wall mid-way all around, is applicable to the cell cultures of tolerance shearing force;

Described cultivation deep hole cross section also can be in circular, quincunx any one.

After adopting the utility model, its beneficial effect is: the deep hole Tissue Culture Plate is by under the external effect that certain rotating speed and eccentricity be provided, nutrient solution in cultivating deep hole forms eddy current, after in the cultivation deep hole of Tissue Culture Plate baffle plate being set, destroyed eddy current, strengthen the nutrient solution vibration, improved the mixed effect of air and nutrient solution, and then improved the volume dissolved oxygen coefficient of deep hole Tissue Culture Plate; Arrange along dislocation on the hole depth direction of described cultivation deep hole between adjacent screen, cause the nutrient solution two adjacent baffle plates of flowing through to change direction, further strengthened the oscillation effect of nutrient solution, improve the volume dissolved oxygen coefficient of deep hole Tissue Culture Plate, reached the volume dissolved oxygen coefficient level of approximation with fermentor tank; The hole wall mid-way arranges the bar shaped baffle plate around four jiaos of cultivation deep holes arrange curved baffle or cultivating deep hole, makes nutrient solution reach different oscillation degree, is applicable to the cell different to the shearing force sensitivity.

Description of drawings

Fig. 1 has 24 hole depth square opening Tissue Culture Plate plan structure synoptic diagram of curved baffle for the utility model;

Fig. 2 is the plan structure synoptic diagram after single square cultivation deep hole amplifies among Fig. 1;

Fig. 3 is that the A-A of Fig. 2 is to structural representation;

Fig. 4 has 24 hole depth square opening Tissue Culture Plate plan structure synoptic diagram of bar shaped baffle plate for the utility model;

Fig. 5 is the plan structure synoptic diagram after single square cultivation deep hole amplifies among Fig. 4;

Fig. 6 is that the C-C of Fig. 5 is to structural representation;

Fig. 7 is that the B-B of Fig. 5 is to structural representation;

Fig. 8 has 48 hole depth square opening Tissue Culture Plate plan structure synoptic diagram of curved baffle for the utility model;

Fig. 9 has 48 hole depth square opening Tissue Culture Plate plan structure synoptic diagram of bar shaped baffle plate for the utility model;

Figure 10 has 96 hole depth square opening Tissue Culture Plate plan structure synoptic diagram of bar shaped baffle plate for the utility model;

Figure 11 has 96 hole depth square opening Tissue Culture Plate plan structure synoptic diagram of curved baffle for the utility model;

Figure 12 has 24 hole depth circular port Tissue Culture Plate plan structure synoptic diagram of curved baffle for the utility model;

Figure 13 has 24 hole depth circular port Tissue Culture Plate plan structure synoptic diagram of bar shaped baffle plate for the utility model.

Embodiment

The embodiment of the invention is example with 24 hole depth porocyte culture plates, only for the explanation concrete grammar, its scale is not subjected to the restriction of embodiment, be applicable to that the Tissue Culture Plate of different holes bit quantity is as 48 hole depth porocyte culture plates and 96 hole depth porocyte culture plates, 48 hole depth porocyte culture plates and 96 its structure of hole depth porocyte culture plate such as Fig. 8, Fig. 9, Figure 10 and shown in Figure 11, be not subjected to simultaneously the restriction of Tissue Culture Plate material, the Tissue Culture Plate material can be by polypropylene, common material such as polystyrene is made, and culture plate can be disposable use or nonexpondable culture plate (autoclave sterilizations of anti-usual conditions).

Embodiment one:

As Fig. 1, Fig. 2 and shown in Figure 3, a kind of 24 hole depth square opening Tissue Culture Plates with baffle plate, it is applicable to the cell cultures to the shearing force sensitivity, it comprises culture plate 1, culture plate 1 is evenly distributed with cultivates deep hole 3, that cultivates deep hole is provided with four

baffle plates

21,22,23,24 interior week, four

baffle plates

21,22,23,24 uniform along side face, adjacent two baffle plates such as baffle plate 21 and baffle plate 23 arrange along dislocation on the hole depth direction of cultivating deep holes, relative two baffle plates such as baffle plate 23 and baffle plate 24 contour being arranged in parallel on the hole depth direction of cultivating deep hole; It is square cultivating deep hole 3 cross sections; Baffle

plate

21,22,23,24 is arc, is arranged to cultivate 3 four jiaos of deep holes.

2 is baffle plate among Fig. 1.

Embodiment two:

As Fig. 4, Fig. 5, Fig. 6 and shown in Figure 7, a kind of 24 hole depth square opening Tissue Culture Plates with baffle plate, it is applicable to the cell cultures of fingerprinting stress, it comprises culture plate 4, culture plate 4 is evenly distributed with cultivates deep hole 6, that cultivates deep hole 6 is provided with four

baffle plates

51,52,53,54 interior week, four

baffle plates

51,52,53,54 uniform along side face, adjacent two baffle plates such as baffle plate 51 and baffle plate 53 arrange along dislocation on the hole depth direction of cultivating deep holes, relative two baffle plates such as baffle plate 51 and baffle plate 52 contour being arranged in parallel on the hole depth direction of cultivating deep hole; It is square cultivating deep hole 6 cross sections; Baffle plate is bar shaped, is arranged to cultivate deep hole hole wall mid-way all around, is applicable to the cell cultures of tolerance shearing force.

5 is baffle plate among Fig. 4.

Implementation column three:

As shown in figure 12, a kind of 24 hole depth circular port Tissue Culture Plates with baffle plate, it is applicable to the cell cultures to the shearing force sensitivity, it comprises culture plate 9, culture plate 9 is evenly distributed with cultivates deep hole 7, cultivate deep hole 7 to be provided with 8, four baffle plates 8 of four baffle plates interior week uniform along side face, adjacent two baffle plates arrange that along dislocation on the hole depth direction of cultivating deep hole 7 two relative baffle plates are contour being arranged in parallel on the hole depth direction of cultivating deep hole; It is circular cultivating deep hole 7 cross sections; Baffle plate 8 is arc.

When 24 hole depth circular port Tissue Culture Plates of band baffle plate are applicable to the cell cultures of fingerprinting stress, as shown in figure 13, only need baffle plate 10 is set to the bar shaped baffle plate, the bar shaped baffle plate is same uniform along cultivating the deep hole side face.

Claims

1. deep hole Tissue Culture Plate with baffle plate, it comprises culture plate, described culture plate is evenly distributed with the cultivation deep hole, it is characterized in that: described cultivation deep hole be provided with four baffle plates interior week, described four baffle plates are uniform along side face, adjacent two described baffle plates are arranged two relative described baffle plates contour being arranged in parallel on the hole depth direction of described cultivation deep hole along dislocation on the hole depth direction of described cultivation deep hole.

2. a kind of deep hole Tissue Culture Plate with baffle plate according to claim 1, it is characterized in that: described cultivation deep hole cross section is square, described square rectangle and the square of comprising.

3. a kind of deep hole Tissue Culture Plate with baffle plate according to claim 2, it is characterized in that: described baffle plate is arc, is arranged at four jiaos of described cultivation deep holes, is applicable to the cultivation to the cell of shearing force sensitivity.

4. a kind of deep hole Tissue Culture Plate with baffle plate according to claim 2, it is characterized in that: described baffle plate is bar shaped, is arranged at hole wall mid-way around the described cultivation deep hole, is applicable to the cell cultures of tolerance shearing force.

5. a kind of deep hole Tissue Culture Plate with baffle plate according to claim 1, it is characterized in that: described cultivation deep hole cross section also can be circle.