CN212451418U - Inoculation disc for rapidly screening strains - Google Patents
Inoculation disc for rapidly screening strains Download PDFInfo
- Publication number
- CN212451418U CN212451418U CN202021052684.7U CN202021052684U CN212451418U CN 212451418 U CN212451418 U CN 212451418U CN 202021052684 U CN202021052684 U CN 202021052684U CN 212451418 U CN212451418 U CN 212451418U
- Authority
- CN
- China
- Prior art keywords
- inoculation
- tray
- screening
- strains
- plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000011081 inoculation Methods 0.000 title claims abstract description 45
- 238000012216 screening Methods 0.000 title claims abstract description 41
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 229910001018 Cast iron Inorganic materials 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 8
- 238000002474 experimental method Methods 0.000 abstract description 7
- 244000005700 microbiome Species 0.000 abstract description 7
- 102000004190 Enzymes Human genes 0.000 abstract description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 5
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 5
- 239000013543 active substance Substances 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 230000003115 biocidal effect Effects 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract description 2
- 238000001228 spectrum Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a strain inoculation dish for rapidly screening strains, which consists of a chassis and an upper plate, wherein the chassis is provided with orderly arranged sample adding holes, the upper plate is provided with a handheld part, and the lower part of the upper plate is provided with orderly arranged inoculation columns, the arrangement mode of the inoculation columns corresponds to the arrangement mode of the sample adding holes, so that the inoculation columns can stretch into the sample adding holes; the diameter of the sample adding hole is 4.5-5.0mm, and the depth of the hole is 5.0 mm; the diameter of the inoculation column is 3.0mm, and the height of the inoculation column is 10.0 mm; 21 different strains can be inoculated at one time for the qualitative primary screening and secondary screening of microorganisms producing certain enzyme; can also be used for the bacteriostasis spectrum experiment of certain bacteriostasis substance or novel antibiotic to various microorganisms; the method is used for qualitative or quantitative experiments of the bacteriostatic activity of the active substances produced by the detection of microorganisms by a flat plate perforation method; can also be used for the experiment research of the confrontation culture among strains, etc.; the workload can be greatly reduced, the experimental materials can be saved, the condition consistency is easy to control, and the result has repeatability.
Description
Technical Field
The invention relates to the technical field of strain inoculation tools, in particular to an inoculation disc for rapidly screening strains.
Background
In the actual work of the strain screening scheme, in order to improve the screening efficiency, the screening work is divided into two steps of primary screening and secondary screening.
The primary screening aims to delete most strains which do not clearly meet the requirements, and retain strains with similar production traits as much as possible, so that excellent strains do not leak the net. Thus, the primary screening work is volume based and the accuracy of the measurement is secondary. The primary screening means should be as fast and simple as possible. The purpose of rescreening is to identify strains that meet production requirements, so the rescreening step is based on quality and should accurately determine the production index of each strain.
The strain screening means must be matched with the requirements of different screening stages, the primary screening is required to be rapid and simple, the secondary screening is required to be accurate, and the measured data can reflect the future production level.
At present, no proper screening tool exists, inoculation work is heavy when large-scale bacterial strain primary screening work is carried out, bacterial strains need to be sampled one by one, the workload is repeated, and the consumption of experimental materials is high.
Disclosure of Invention
The invention aims to provide a inoculation tray for rapidly screening strains, which can solve the problem of heavy workload during primary screening of a large number of strains, reduce repeated workload and save experimental materials.
The technical scheme is as follows:
a strain inoculation tray for rapidly screening strains is composed of a base plate and an upper plate, wherein the base plate is provided with sample adding holes which are arranged in order, the plate of the upper plate is provided with a handheld part, inoculation columns which are arranged in order are arranged below the upper plate, and the arrangement mode of the inoculation columns corresponds to the arrangement mode of the sample adding holes, so that the inoculation columns can extend into the sample adding holes; the diameter of the sample adding hole is 4.5-5.0mm, and the depth of the hole is 5.0 mm; the diameter of the inoculation column is 3.0mm, and the height is 10.0 mm.
Preferably, the diameters of the disc surfaces of the bottom disc and the upper disc are 90.0mm, the thickness of the bottom disc is 8mm, and the thickness of the upper disc is 5 mm.
Preferably, the number of the loading wells and the number of the seeding columns are 21.
Preferably, the handheld portion is cylindric, and cylindric upper and lower end straight radial diminishes gradually to the waist within a definite time, and its diameter 10.0mm, height 10.0mm are favorable to taking the convenience, prevent the slippage.
Preferably, the base plate and the upper plate can meet the requirements of dry heat sterilization (160 ℃, 2 h) or high-pressure steam sterilization (121 ℃, 30 min), and the specific materials are made of stainless steel or cast iron materials.
Preferably, the inoculation column is connected to the upper tray by a threaded arrangement.
Has the advantages that:
21 different strains can be inoculated at one time for the qualitative primary screening and secondary screening of microorganisms producing certain enzyme; can also be used for the bacteriostasis spectrum experiment of certain bacteriostasis substance or novel antibiotic to various microorganisms; the method is used for qualitative or quantitative experiments of the bacteriostatic activity of the active substances produced by the detection of microorganisms by a flat plate perforation method; can also be used for the experiment research of the confrontation culture among strains. The workload can be greatly reduced, the experimental materials can be saved, the condition consistency is easy to control, and the result has repeatability. In order to realize heavy inoculation screening work during large-batch bacterial strain primary screening work during related scientific research of microorganisms, the tool can realize one-time inoculation of at most 20-21 bacterial suspensions or test liquid, and realize rapid qualitative primary screening.
Meanwhile, the uniformly distributed inoculation columns on the plate can be used as a multi-point puncher of a flat plate and quantitative experiments, so that two purposes of one tool are realized.
Drawings
Fig. 1 is a schematic view of the chassis structure.
Fig. 2 is a side view of the chassis.
Fig. 3 is a bottom view of the upper tray.
Figure 4 is a top view of the upper plate.
FIG. 5 is a side view of the upper plate.
Fig. 6 is a combined usage diagram.
In the figure:
1. the device comprises a base plate, 2, an upper plate, 3, a sample adding hole, 4, an inoculation column, 5 and a handheld part.
Detailed Description
The present invention is further illustrated by the following specific examples, which are intended to be illustrative only and not limiting.
As shown in fig. 1-6, a seed plate for rapid screening of bacterial strains, which comprises a base plate 1 and an upper plate 2, wherein the base plate 1 is provided with orderly arranged sample adding holes 3, the plate of the upper plate 2 is provided with a handheld part 5, orderly arranged inoculation columns 4 are arranged below the upper plate 2, and the arrangement of the inoculation columns 4 corresponds to the arrangement of the sample adding holes 3, so that the inoculation columns 4 can extend into the sample adding holes 3; the inner diameter of the sampling hole 3 is 4.5-5.0mm, and the hole depth is 5.0 mm; the diameter of the inoculation column is 3.0mm, and the height is 10.0 mm.
The disc surfaces of the base plate 1 and the upper disc 2 are 90.0mm in diameter, the thickness of the base plate 1 is 8mm, and the thickness of the upper disc 2 is 5 mm.
The number of the loading holes 3 and the inoculation columns 4 is 21.
The handheld portion is cylindric, and cylindric upper and lower end straight becomes little gradually to the waist, and its diameter 10.0mm, height 10.0mm are favorable to taking the convenience, prevent the slippage.
The base plate and the upper plate can meet the requirements of dry heat sterilization (160 ℃, 2 h) or high-pressure steam sterilization (121 ℃, 30 min), and the specific materials are made of stainless steel or cast iron materials.
The inoculation column 4 is connected to the upper tray 2 by a screw thread arrangement.
Example one: enzyme-producing strain screening
Inoculating a plurality of bacteria separated from a marine environment into an LB culture medium test tube, and culturing for 24 h; in a purification workbench, taking a sterilized multi-strain inoculation tray base plate, sequentially adding liquid culture solution 20u L of different strains into sample adding holes from row A, after all the sample adding of 21 holes is finished, taking a sterilized multi-strain inoculation tray and hanging the tray, aligning an inoculation column to the corresponding sample adding holes according to A-A, B-B, C-C, D-D, E-E, placing the inoculation column on the base plate for sampling, taking the tray and putting the tray on a prepared enzyme production screening culture medium flat plate (the bottom of the flat plate is also marked with the line marks A-E in advance), finishing inoculation, taking down an inoculator, covering a dish cover, standing for 10min, allowing the inoculation solution to permeate into the culture medium, then carrying out inverted culture, observing the existence and the size of an enzymolysis transparent ring, and primarily screening out the enzyme production strains.
Example two: screening microbial strains capable of generating bacteriostatic active substances for certain pathogenic microorganisms
Separating and purifying a plurality of test strains, taking 1mL of liquid culture, placing the liquid culture in a 1.5mL LEPPendorf tube, centrifuging at a high speed, taking supernatant 20u L, adding the supernatant into a sample adding hole on the bottom plate of an inoculation tray, and placing the upper tray at the aligned position A-A, B-B, C-C, D-D, E-E. Taking out the upper plate of the inoculation tray, correspondingly marking the upper plate on a flat plate (the bottom of the flat plate is marked with the line mark A-E in advance) which is mixed or coated with pathogen indicating bacteria with certain concentration, taking down the upper plate, covering the upper plate with a tray cover, standing for 10min, allowing liquid to permeate into a culture medium, then performing inverted culture, observing the existence and the size of a bacteriostatic ring around the spotting, and primarily screening strains producing bacteriostatic active substances.
Example three: the upper plate of the inoculation plate can be used as a porous puncher for manufacturing a porous flat plate
10mL of sterile water agar is poured into a sterile culture dish to be solidified to prepare a bottom plate, then a culture medium (or substances such as bacteria with a certain concentration or antibiotics with a certain concentration and the like which are mixed according to experimental design) which is cooled to 40 ℃ is poured into the sterile culture dish, then the sterile inoculation dish is put into the sterile culture dish, after solidification, the inoculator is pulled out to form a porous plate with 21 uniform positions and consistent holes, and then quantitative sample adding can be carried out in the plate holes, so that quantitative tests of certain qualitative screening tests can be realized.
Claims (6)
1. The inoculation tray for rapidly screening the strains is characterized by comprising a base tray (1) and an upper tray (2), wherein the base tray (1) is provided with orderly arranged sample adding holes (3), the tray of the upper tray (2) is provided with a handheld part (5), orderly arranged inoculation columns (4) are arranged below the upper tray (2), and the arrangement mode of the inoculation columns (4) corresponds to the arrangement mode of the sample adding holes (3), so that the inoculation columns (4) can extend into the sample adding holes (3); the inner diameter of the sampling hole (3) is 4.5-5.0mm, and the hole depth is 5.0 mm; the diameter of the inoculation column is 3.0mm, and the height is 10.0 mm.
2. A seed plate for rapid screening of strains according to claim 1, characterized in that the diameter of the plate surface of the bottom plate (1) and the upper plate (2) is 90.0mm, the thickness of the bottom plate (1) is 8mm, and the thickness of the upper plate (2) is 5 mm.
3. A seed plate for rapid screening of strains according to claim 1, characterized in that the number of wells (3) and columns (4) is 21.
4. The inoculation tray for rapid screening of strains according to claim 1, wherein the hand-held portion (5) is cylindrical, and the upper and lower ends of the cylindrical shape are gradually reduced toward the waist, and the diameter of the cylindrical shape is 10.0mm, and the height of the cylindrical shape is 10.0 mm.
5. A seed plate for rapid strain screening according to claim 1, characterized in that the bottom plate (1) and the upper plate (2) can satisfy dry heat sterilization or high pressure steam sterilization, and are made of stainless steel or cast iron.
6. A seed tray for rapid screening of strains according to claim 1, characterized in that the inoculation column (4) is connected to the upper tray (2) by a screw structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021052684.7U CN212451418U (en) | 2020-06-10 | 2020-06-10 | Inoculation disc for rapidly screening strains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021052684.7U CN212451418U (en) | 2020-06-10 | 2020-06-10 | Inoculation disc for rapidly screening strains |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212451418U true CN212451418U (en) | 2021-02-02 |
Family
ID=74491756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021052684.7U Active CN212451418U (en) | 2020-06-10 | 2020-06-10 | Inoculation disc for rapidly screening strains |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212451418U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114437910A (en) * | 2022-01-24 | 2022-05-06 | 内蒙古自治区农牧业科学院 | Hand-held type many fallers for microorganism are cultivateed in quick duplicate transfer |
-
2020
- 2020-06-10 CN CN202021052684.7U patent/CN212451418U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114437910A (en) * | 2022-01-24 | 2022-05-06 | 内蒙古自治区农牧业科学院 | Hand-held type many fallers for microorganism are cultivateed in quick duplicate transfer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN212451418U (en) | Inoculation disc for rapidly screening strains | |
| CN105255715A (en) | High-pass multipurpose controllable microorganism inoculation device and batch strain inoculation method | |
| CN204758370U (en) | Nitrogen blowing apparatus | |
| CN203212577U (en) | Deep-hole cell culture plate with baffle plates | |
| CN110542747A (en) | Water quality detection device for aquatic product culture | |
| CN202471693U (en) | Novel air pressure consolidometer | |
| CN209311138U (en) | It is fermented based on excrement colibacillus group multitube and detects more water layer sampling apparatuses | |
| CN207742253U (en) | A kind of oxygen sensor chip positioning resistance testing device | |
| CN212133551U (en) | Laboratory antibacterial zone area measurement analyzer | |
| CN103642691B (en) | Deep-hole cell culture plate | |
| CN201501859U (en) | Fungal microculture device capable of being directly observed under microscope | |
| CN204125459U (en) | A kind of biological culture laboratory apparatus being convenient to observe | |
| CN208049996U (en) | A kind of conveniently test tube for food detection | |
| CN201006428Y (en) | Sulphate reduction bacterium testing bottle | |
| CN220244078U (en) | PCR kit holder | |
| CN205269736U (en) | Slant culture base rack | |
| CN107012233A (en) | A kind of fluorescence probe quantitative PCR quick determination method of bulk bacteria | |
| CN208003973U (en) | A kind of use for laboratory organic reagent quantifies liquid-transfering device | |
| CN206014877U (en) | The quick paster apparatus of paper disc method | |
| CN211620491U (en) | Culture flat plate for bacteriostatic zone experiment | |
| CN221726052U (en) | Convenient sample loading card | |
| CN201241126Y (en) | Disposable microorganism test board | |
| CN215209438U (en) | Fluorescent detection device for strain breeding | |
| CN213803716U (en) | Novel culture dish frame | |
| CN111595229B (en) | Laboratory antibacterial zone area measurement analyzer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: MARINE RESOURCES DEVELOPMENT INSTITUTE OF JIANGSU (LIANYUNGANG) Assignor: Jiangsu Ocean University Contract record no.: X2023980048267 Denomination of utility model: A inoculum tray for rapid screening of bacterial strains Granted publication date: 20210202 License type: Common License Record date: 20231128 |
|
| EC01 | Cancellation of recordation of patent licensing contract | ||
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: MARINE RESOURCES DEVELOPMENT INSTITUTE OF JIANGSU (LIANYUNGANG) Assignor: Jiangsu Ocean University Contract record no.: X2023980048267 Date of cancellation: 20240726 |