CN103642690A - Plate cover structure of cell culture plate - Google Patents
Plate cover structure of cell culture plate Download PDFInfo
- Publication number
- CN103642690A CN103642690A CN201310667873.3A CN201310667873A CN103642690A CN 103642690 A CN103642690 A CN 103642690A CN 201310667873 A CN201310667873 A CN 201310667873A CN 103642690 A CN103642690 A CN 103642690A
- Authority
- CN
- China
- Prior art keywords
- plate
- tissue culture
- ventpipe
- culture plate
- lid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000004113 cell culture Methods 0.000 title abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000741 silica gel Substances 0.000 claims abstract description 5
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 5
- 235000015097 nutrients Nutrition 0.000 claims description 21
- 239000006052 feed supplement Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 229960001866 silicon dioxide Drugs 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 7
- 239000001301 oxygen Substances 0.000 abstract description 7
- 229910052760 oxygen Inorganic materials 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 230000003373 anti-fouling effect Effects 0.000 abstract 1
- 238000012864 cross contamination Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 12
- 230000000694 effects Effects 0.000 description 5
- 210000004349 growth plate Anatomy 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a plate cover structure of a cell culture plate. The volume coefficient of dissolved oxygen of the cell culture plate can be improved, high-density cultivation is achieved, the expression quantity of a protein product is increased, and cross contamination of a culture solution can be effectively avoided. The plate cover structure of the cell culture plate comprises a plate cover, wherein an anti-fouling membrane, a waterproof membrane and a silica gel plate are orderly arranged inside the plate cover from top to bottom; a plurality of culture deep holes are evenly distributed on the cell culture plate. The plate cover structure is characterized in that a pipe body is inserted on the plate cover corresponding to each culture deep hole; the culture deep holes are communicated with the external part of the cell culture plate by the pipe body; the pipe body comprises a breather pipe, an exhaust pipe and a feeding pipe.
Description
Technical field
Patent of the present invention relates to cell culture apparatus technical field in biology, relates in particular to the deep hole culture plate that cell cultures is used, and is specially the plate lid structure of Tissue Culture Plate.
Background technology
Along with molecular biological development, the heterogenous expression technology of albumen is ripe and be widely used gradually.The expression of foreign protein need to be selected suitable expressive host and Expression element, and this just need to build huge expression library, therefrom selects preferably representation aids box.Tissue Culture Plate can utilize less nutrient solution to carry out the screening of high-flux cell cultivation and bacterial classification, cell strain and expression library, has fast, high-throughput, the feature such as cost-saving.And in the expression process of Study of Exogenous albumen, as antibody, enzyme, should be to cultivate to raise the efficiency to obtain again enough albumen and carry out analyzing and testing by corpusculum, this just need to further realize high-density culture.
The corpusculum of existing fermentor tank Sub-scale is that its volume dissolved oxygen coefficient of culture apparatus is little, has limited the high-density culture of bacterial classification or cell, and has reduced protein product expression amount.
Summary of the invention
For the problems referred to above, patent of the present invention provides the plate lid structure of Tissue Culture Plate, and it can improve the volume dissolved oxygen coefficient of Tissue Culture Plate, realizes high-density culture, increases protein product expression amount, and can effectively avoid nutrient solution crossed contamination.
Its technical scheme is such, the plate lid structure of Tissue Culture Plate, comprise plate lid, in described plate lid, from top to down anti-soil film, waterproof membrane and silica-gel plate be installed successively, on Tissue Culture Plate, be evenly equipped with some cultivation deep holes, it is characterized in that: corresponding cultivate deep hole described in each, described plate covers and is fitted with body, described body is by the outside UNICOM of described cultivation deep hole and described Tissue Culture Plate, and described body comprises ventpipe, vapor pipe and feed supplement pipe.
It is further characterized in that:
Be positioned at the below of the nutrient solution liquid level of described cultivation deep hole, ventpipe is provided with baffle plate, described baffle plate is provided with four, and circumferentially uniform along ventpipe, adjacent two described baffle plates are arranged along ventpipe dislocation, two relative described baffle plates are arranged in parallel in described ventpipe direction, and the ventpipe of one end, plate lid outside is connected with extraneous gas supply line;
Be positioned at described plate lid outside, described feed supplement pipe is provided with branch road, and described branch road quantity is more than or equal to 2;
Described branch road is provided with three, and three branch roads are connected with the pipeline of extraneous nutrient solution, acid solution, alkali lye respectively, by outer bound pair nutrient solution, are realized pH regulator and are added the feed supplements such as nutrient solution;
The lower surface of described vapor pipe and described feed supplement pipe is positioned at the top of the nutrient solution liquid level of described cultivation deep hole, does not contact with nutrient solution.
Adopt after patent growth plate of the present invention lid, its beneficial effect is: the ventpipe covering by plate can provide thalli growth necessary oxygen to enough in nutrient solution, realizes high-density culture, and improves protein product expression amount; Metabolism waste gas and the oxygen of underusing are discharged by vapor pipe; By supplemented medium and nutritive substance in feed supplement Guan Kexiang nutrient solution, add acid or alkali and regulate nutrient solution pH, can pass through the control of exterior tubing, flow pump, realize the dropping of microlitre level; Anti-soil film in plate lid plays and prevents that miscellaneous bacteria, dust etc. from polluting the effect of fermented liquid, and waterproof membrane plays the effect that prevents nutrient solution moisture evaporation, and silica-gel plate can effectively prevent the crossed contamination between culture hole; Under vibrator provides certain rotating speed and eccentricity effect, the baffle plate arranging on ventpipe plays the effect of smashing bubble in nutrient solution, oxygen is dissolved in nutrient solution, has improved the volume dissolved oxygen coefficient of Tissue Culture Plate, and the effect that nutrient solution is played stirring, mixed.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is assembly structure schematic diagram of the present invention.
Embodiment
Patent of the present invention be take the structure of 24 hole depth well culture plate plates lids and is described as example, the structure of patent of the present invention is not limited to 24 deep hole growth plate lids, be equally applicable to the growth plate lid of 48 hole depth well culture plate plate lids, 96 hole depth well culture plate plate lids and other hole bit quantity, equally, the structure of patent of the present invention is applicable to the plate lid of the culture plate of position, different shapes hole.
As Fig. 1, shown in Fig. 2, the plate lid structure of Tissue Culture Plate, comprise plate lid 1, in plate lid 1, anti-soil film 2 is installed from top to down successively, waterproof membrane 3 and silica-gel plate 4, on Tissue Culture Plate, be evenly equipped with some cultivation deep holes 9, wherein, in Fig. 2, only illustrate one and cultivate deep hole, corresponding each cultivation deep hole 9, plate covers and is fitted with body, body will be cultivated the outside UNICOM of deep hole and Tissue Culture Plate, body comprises ventpipe 6, vapor pipe 5 and feed supplement pipe 7, be positioned at nutrient solution liquid level 11 belows of cultivating deep hole 9, ventpipe 5 is provided with baffle plate 10, baffle plate 10 is provided with four, and circumferentially uniform along ventpipe 5, adjacent two baffle plates are arranged along ventpipe dislocation, two relative baffle plates are arranged in parallel in ventpipe direction, be positioned at plate and cover 1 outside, feed supplement pipe 7 is provided with three branch roads 8, three branch roads can be respectively and extraneous nutrient solution, acid solution, the pipeline of alkali lye is connected, the lower surface of vapor pipe 5 and feed supplement pipe 7 is positioned at nutrient solution liquid level 11 tops of cultivating deep hole 9.
Claims (5)
1. the plate of Tissue Culture Plate covers structure, comprise plate lid, in described plate lid, from top to down anti-soil film, waterproof membrane and silica-gel plate be installed successively, on Tissue Culture Plate, be evenly equipped with some cultivation deep holes, it is characterized in that: corresponding cultivate deep hole described in each, described plate covers and is fitted with body, and described body is by the outside UNICOM of described cultivation deep hole and described Tissue Culture Plate, and described body comprises ventpipe, vapor pipe and feed supplement pipe.
2. the plate of Tissue Culture Plate according to claim 1 covers structure, it is characterized in that: the below that is positioned at the nutrient solution liquid level of described cultivation deep hole, ventpipe is provided with baffle plate, described baffle plate is provided with four, and circumferentially uniform along ventpipe, adjacent two described baffle plates are along ventpipe dislocation layout, and two relative described baffle plates are arranged in parallel in described ventpipe direction, and the ventpipe of one end, plate lid outside is connected with extraneous gas supply line.
3. the plate of Tissue Culture Plate according to claim 2 lid structure, is characterized in that: be positioned at described plate lid outside, described feed supplement pipe is provided with branch road, and described branch road quantity is more than or equal to 2.
4. the plate of Tissue Culture Plate according to claim 3 lid structure, is characterized in that: described branch road is provided with three, is connected with the pipeline of extraneous nutrient solution, acid solution, alkali lye respectively.
5. the plate of Tissue Culture Plate according to claim 1 lid structure, is characterized in that: the lower surface of described vapor pipe and described feed supplement pipe is positioned at the top of the nutrient solution liquid level of described cultivation deep hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310667873.3A CN103642690B (en) | 2013-12-11 | 2013-12-11 | The plate lid structure of Tissue Culture Plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310667873.3A CN103642690B (en) | 2013-12-11 | 2013-12-11 | The plate lid structure of Tissue Culture Plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103642690A true CN103642690A (en) | 2014-03-19 |
| CN103642690B CN103642690B (en) | 2015-09-02 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310667873.3A Expired - Fee Related CN103642690B (en) | 2013-12-11 | 2013-12-11 | The plate lid structure of Tissue Culture Plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103642690B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4142940A (en) * | 1975-09-13 | 1979-03-06 | W. C. Heraeus Gmbh | Cultivation containers for cultures, microorganisms, cells, tissue and the like |
| CN202030764U (en) * | 2011-05-06 | 2011-11-09 | 安徽丰原生物化学股份有限公司 | Fermentation bottle and bio-fermentation culture device provided with same |
| CN103013829A (en) * | 2012-12-28 | 2013-04-03 | 江南大学 | Structure of deep-well cell culture plate cover |
| CN203212577U (en) * | 2012-12-28 | 2013-09-25 | 江南大学 | Deep-hole cell culture plate with baffle plates |
-
2013
- 2013-12-11 CN CN201310667873.3A patent/CN103642690B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4142940A (en) * | 1975-09-13 | 1979-03-06 | W. C. Heraeus Gmbh | Cultivation containers for cultures, microorganisms, cells, tissue and the like |
| CN202030764U (en) * | 2011-05-06 | 2011-11-09 | 安徽丰原生物化学股份有限公司 | Fermentation bottle and bio-fermentation culture device provided with same |
| CN103013829A (en) * | 2012-12-28 | 2013-04-03 | 江南大学 | Structure of deep-well cell culture plate cover |
| CN203212577U (en) * | 2012-12-28 | 2013-09-25 | 江南大学 | Deep-hole cell culture plate with baffle plates |
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| Publication number | Publication date |
|---|---|
| CN103642690B (en) | 2015-09-02 |
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