CN103013829A - Structure of deep-well cell culture plate cover - Google Patents
Structure of deep-well cell culture plate cover Download PDFInfo
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- CN103013829A CN103013829A CN201210580641XA CN201210580641A CN103013829A CN 103013829 A CN103013829 A CN 103013829A CN 201210580641X A CN201210580641X A CN 201210580641XA CN 201210580641 A CN201210580641 A CN 201210580641A CN 103013829 A CN103013829 A CN 103013829A
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Abstract
The invention provides a structure of a deep-well cell culture plate cover, which can effectively avoid pollution and ensure favorable air permeability among culture plates. The structure comprises a stainless steel plate cover, an anti-pollution membrane, a waterproof membrane and a silica gel plate. The invention is characterized in that two side walls of the stainless steel plate cover are respectively provided with clamping grooves; the anti-pollution membrane, waterproof membrane and silica gel plate are sequentially clamped in the clamping grooves from top to bottom; and four corners of the upper end surface of the stainless steel plate cover are respectively provided with a bulge.
Description
Technical field
The present invention relates to cell culture apparatus technical field in the biology, relate in particular to the deep hole culture plate that cell cultures is used, be specially the structure of deep hole Tissue Culture Plate plate lid.
Background technology
At present; carry out in the biology that high-flux cell is cultivated and the early stage processing parameter screening study of fermentation engineering amplification process in often can use culture plate; culture plate has all supported the use the plate lid; the conventional panels lid is " sandwich " structure; as shown in Figure 1; namely by stainless steel plate lid 1; waterproof membrane 3; anti-soil film 2 and silica-gel plate 4 consist of; during use; with anti-soil film 2; waterproof membrane 3 and silica-gel plate 4 serve as a contrast from top to bottom successively to the groove of stainless steel plate lid 1; again stainless steel plate lid 1 is buckled on the culture plate 8; then apply external force so that silica-gel plate 4 compressions; and closely be adjacent to culture plate 8 so that completely cut off between each culture hole 7 in the culture plate, prevent from the mutual pollution between each culture hole 7 and can reduce in the culturing process evaporating.Smooth in order to keep serving as a contrast the anti-soil film 2, waterproof membrane 3 and the silica-gel plate 4 that cover in 1 at stainless steel plate, the size of anti-soil film 2, waterproof membrane 3 and silica-gel plate 4 all is slightly less than the size that stainless steel plate covers 1 groove, when the growth plate lid uses, anti-soil film 2, waterproof membrane 3 and silica-gel plate 4 come off in groove easily, pollute, also can owing to silica-gel plate 4 is subjected to force compresses, produces the local bending fold, so that be not adjacent between silica-gel plate 4 and the culture hole 7, can cause mutually polluting between each culture hole 7; Simultaneously, it is the plane that stainless steel plate covers 1 top, when cell cultures, a plurality of culture plates 8 are superimposed, and culture plate 8 bottom surfaces in upper strata can be blocked the ventilating pit of lower floor's stainless steel plate lid 1, and ventilation property is bad, simultaneously, before use, stainless steel plate lid 1 is inverted and is placed on the experiment table, and the ventilating pit 5 of stainless steel plate lid 1 directly contacts the experiment table top, contaminated easily, and then causing the pollution of fermented liquid, among Fig. 1,6 is the ventilating pit of silica-gel plate 4.
Summary of the invention
For the problems referred to above, the invention provides the structure of deep hole Tissue Culture Plate plate lid, it can effectively avoid polluting, and guarantees good air permeability between the culture plate.
Its technical scheme is such, it comprises stainless steel plate lid, anti-soil film, waterproof membrane and silica-gel plate, it is characterized in that: described stainless steel plate lid two side is provided with draw-in groove, described anti-soil film, described waterproof membrane and described silica-gel plate are installed in the described draw-in groove from top to bottom successively, and described stainless steel plate covers four jiaos of end faces and is provided with projection.
It is further characterized in that:
Described silica-gel plate bottom be provided with culture plate in culture hole plug one to one, described plug center is provided with vent-hole, has the space between the adjacent described plug;
Described projection is truncated rectangular pyramids shape.
After adopting structure of the present invention, its beneficial effect is: anti-soil film, waterproof membrane and silica-gel plate are installed in the draw-in groove of stainless steel plate lid dual-side, prevent that effectively anti-soil film, waterproof membrane and silica-gel plate from coming off; The top of stainless steel plate lid is provided with truncated rectangular pyramids shape projection, so that leave the space between the culture plate bottom, culture plate upper strata that is stacked together and the lower floor's stainless steel plate lid, guarantee the good air permeability of culture plate, simultaneously, when stainless steel plate lid upside down is on experiment table, truncated rectangular pyramids shape projection can avoid the stainless steel plate cover to pollute, and guarantees that fermented liquid is not contaminated; Be provided with the plug of each culture hole of corresponding culture plate in the silica-gel plate bottom, can further prevent from mutually polluting between the culture hole.
Description of drawings
Fig. 1 is the structural representation of the supporting plate lid of traditional culture plate;
Fig. 2 is structural representation of the present invention;
Fig. 3 is the structural representation of looking up of silica-gel plate of the present invention;
Fig. 4 is that the A-A of Fig. 3 is to cross-sectional view;
Fig. 5 is the structural representation of looking up of stainless steel plate lid of the present invention;
Fig. 6 is that the C-C of Fig. 5 is to cross-sectional view;
Fig. 7 is that the B-B of Fig. 5 is to cross-sectional view;
Fig. 8 is the plan structure synoptic diagram of stainless steel plate lid of the present invention;
Fig. 9 is that the D-D of Fig. 8 is to cross-sectional view.
Embodiment
The present invention describes as an example of the structure of 24 hole depth porocyte growth plate lids example, structure of the present invention is not limited to 24 deep hole growth plate lid, be equally applicable to the growth plate lid of 48 hole depth well culture plate plates lid, 96 hole depth well culture plate plates lid and other hole bit quantity, equally, structure of the present invention is applicable to the culture plate cover plate of position, different shapes hole, and its plug shape can change according to concrete culture plate deep hole shape.
Extremely shown in Figure 9 such as Fig. 2, the structure of deep hole Tissue Culture Plate plate lid, it comprises stainless steel plate, 9, anti-soil film 2, waterproof membrane 3 and silica-gel plate 11, stainless steel plate covers 9 two sides and is provided with draw-in groove 14, anti-soil film 2, waterproof membrane 3 and silica-gel plate 11 are installed on successively stainless steel plate from top to bottom and cover in the 9 two side draw-in grooves 14, stainless steel plate covers four jiaos of 9 upper surfaces and is provided with projection 10, silica-gel plate 11 bottoms are provided with and culture plate 8 interior culture hole 7 plug 12 one to one, plug 12 centers are provided with vent-hole 6, have space 13 between the adjacent plug; The projection 10 of stainless steel plate lid 9 is truncated rectangular pyramids shape.
Claims (3)
1. the structure of deep hole Tissue Culture Plate plate lid, it comprises stainless steel plate lid, anti-soil film, waterproof membrane and silica-gel plate, it is characterized in that: described stainless steel plate lid two side is provided with draw-in groove, described anti-soil film, described waterproof membrane and described silica-gel plate are installed in the described draw-in groove from top to bottom successively, and described stainless steel plate covers four jiaos of end faces and is provided with projection.
2. the structure of deep hole Tissue Culture Plate plate according to claim 1 lid, it is characterized in that: described silica-gel plate bottom be provided with culture plate in culture hole plug one to one, described plug center is provided with vent-hole, has the space between the adjacent described plug.
3. the structure of deep hole Tissue Culture Plate plate according to claim 1 lid, it is characterized in that: described projection is truncated rectangular pyramids shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210580641.XA CN103013829B (en) | 2012-12-28 | 2012-12-28 | Structure of deep-well cell culture plate cover |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210580641.XA CN103013829B (en) | 2012-12-28 | 2012-12-28 | Structure of deep-well cell culture plate cover |
Publications (2)
| Publication Number | Publication Date |
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| CN103013829A true CN103013829A (en) | 2013-04-03 |
| CN103013829B CN103013829B (en) | 2015-04-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210580641.XA Active CN103013829B (en) | 2012-12-28 | 2012-12-28 | Structure of deep-well cell culture plate cover |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642690A (en) * | 2013-12-11 | 2014-03-19 | 江南大学 | Plate cover structure of cell culture plate |
| CN104371921A (en) * | 2014-12-09 | 2015-02-25 | 江南大学 | Press-fitting structure for multi-layer tissue culture plates |
| CN104371923A (en) * | 2014-12-09 | 2015-02-25 | 江南大学 | Cell culture plate and plate cover structure thereof |
| CN110023480A (en) * | 2016-12-14 | 2019-07-16 | 株式会社日立高新技术 | Culture apparatus |
Citations (4)
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|---|---|---|---|---|
| US4142940A (en) * | 1975-09-13 | 1979-03-06 | W. C. Heraeus Gmbh | Cultivation containers for cultures, microorganisms, cells, tissue and the like |
| CN102268369A (en) * | 2010-06-03 | 2011-12-07 | 中国航天员科研训练中心 | Gas exchange type cell culture bottle |
| CN102481572A (en) * | 2009-09-05 | 2012-05-30 | 英国龙沙生物医药股份有限公司 | Deepwell plate system with lid |
| CN203065490U (en) * | 2012-12-28 | 2013-07-17 | 江南大学 | Structure of cover of deep hole cell culture plate |
-
2012
- 2012-12-28 CN CN201210580641.XA patent/CN103013829B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4142940A (en) * | 1975-09-13 | 1979-03-06 | W. C. Heraeus Gmbh | Cultivation containers for cultures, microorganisms, cells, tissue and the like |
| CN102481572A (en) * | 2009-09-05 | 2012-05-30 | 英国龙沙生物医药股份有限公司 | Deepwell plate system with lid |
| CN102268369A (en) * | 2010-06-03 | 2011-12-07 | 中国航天员科研训练中心 | Gas exchange type cell culture bottle |
| CN203065490U (en) * | 2012-12-28 | 2013-07-17 | 江南大学 | Structure of cover of deep hole cell culture plate |
Non-Patent Citations (2)
| Title |
|---|
| 上海甘薇生物科技有限公司: "孔板培养微生物专用三明治盖（深孔板板盖）", 《网址：HTTP://WWW.BIOMART.CN/INFOSUPPLY/6250356.HTM》, 25 July 2012 (2012-07-25), pages 1 - 2 * |
| 北京优尼康生物科技有限公司: "48孔细胞培养板", 《网址：HTTP://WWW.LABBASE.NET/SUPPLY/SUPPLYITEMS-2703966.HTML》, 13 November 2012 (2012-11-13), pages 1 - 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642690A (en) * | 2013-12-11 | 2014-03-19 | 江南大学 | Plate cover structure of cell culture plate |
| CN104371921A (en) * | 2014-12-09 | 2015-02-25 | 江南大学 | Press-fitting structure for multi-layer tissue culture plates |
| CN104371923A (en) * | 2014-12-09 | 2015-02-25 | 江南大学 | Cell culture plate and plate cover structure thereof |
| CN110023480A (en) * | 2016-12-14 | 2019-07-16 | 株式会社日立高新技术 | Culture apparatus |
| CN110023480B (en) * | 2016-12-14 | 2022-11-11 | 株式会社日立高新技术 | Culture instrument |
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| Publication number | Publication date |
|---|---|
| CN103013829B (en) | 2015-04-22 |
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