CN104371921A - Press-fitting structure for multi-layer tissue culture plates - Google Patents
Press-fitting structure for multi-layer tissue culture plates Download PDFInfo
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- CN104371921A CN104371921A CN201410744648.XA CN201410744648A CN104371921A CN 104371921 A CN104371921 A CN 104371921A CN 201410744648 A CN201410744648 A CN 201410744648A CN 104371921 A CN104371921 A CN 104371921A
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- tissue culture
- culture plate
- transverse axis
- plate
- mounting structure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention provides a press-fitting structure for multi-layer tissue culture plates. By the press-fitting structure, multiple tissue culture plates which are stacked can be pressed and fixed onto a shaking table, leakproofness and air flowability of plate covers and culture plate bodies of shallow-hole tissue culture plates are ensured, and meanwhile vertical space of the shaking table is utilized effectively. The press-fitting structure comprises a baseplate, a plurality of tissue culture plates are stacked and juxtaposed on the baseplate, a pressing mechanism is arranged on the baseplate and comprises cross shafts, supports, fixing shafts, shaft sleeves and fixing mechanisms, each cross shaft is arranged over the corresponding tissue culture plates in parallel, two ends of each cross shaft are supported through one group of the supports, the supports are fixed on the baseplate, each cross shaft is provided with the shaft sleeve, each fixing shaft is mounted in the corresponding shaft sleeve and capable of moving up and down along the vertical direction, the lower end of each fixing shaft is provided with a pressure pad, each fixing mechanism limits up-down movement of each fixing shaft along the vertical direction, and supports are arranged on the plate cover of each tissue culture plate.
Description
Technical field
The present invention relates to the stationary installation of Tissue Culture Plate, be specifically related to the press mounting structure of multi-layer cellular culture plate.
Background technology
Along with molecular biological development, the heterogenous expression technology of albumen is ripe gradually and be widely used.The expression of foreign protein needs to select suitable expressive host and Expression element, and this just needs to build huge expression library, therefrom selects preferably representation aids box.Tissue Culture Plate can utilize less nutrient solution to carry out the screening of high-flux cell cultivation and bacterial classification, cell strain and expression library, has fast, high-throughput, the feature such as cost-saving.
Existing Tissue Culture Plate is in use in order to avoid crossed contamination between microbiological contamination or plate hole, need to seal plate mouth, now mainly contain two kinds of sealing plate mouth modes, one is that latex mattress is sealed, one is the sealing of " sandwich " formula plate lid, " sandwich " formula plate lid well can carry out gaseous interchange, avoids crossed contamination.But stopping property cannot ensure when plate lid and orifice plate are combined, and need specifically to cultivate shaking table.And the size in orifice plate shaking table plate face when shaking table is cultivated has limited to the orifice plate number that can cultivate simultaneously.And shaking table solid space can not be utilized effectively.
Summary of the invention
For the problems referred to above, the invention provides the press mounting structure of multi-layer cellular culture plate, multiple Tissue Culture Plates after stacking can be fixed in wanting on bed by it, effectively make use of again the vertical space of shaking table while guaranteeing shallow bore hole Tissue Culture Plate plate lid and culture plate body seal and air flow property.
Its technical scheme is such, comprise base plate, some Tissue Culture Plates stack and are placed on described base plate, described base plate is provided with hold-down mechanism, described hold-down mechanism comprises transverse axis, support, stationary shaft and axle sleeve, described transverse axis is set in parallel in above Tissue Culture Plate, its two ends are supported by a pack support, described support is fixed on base plate, described transverse axis is provided with axle sleeve, described stationary shaft is installed on described axle sleeve, and can vertically go up, lower movement, the lower end of described stationary shaft is provided with pressure pad, also comprise the described stationary shaft of restriction vertically to go up, the fixed mechanism of lower movement, the plate of described Tissue Culture Plate covers and is provided with supporter, the upper surface of described supporter is plane, and be parallel to the upper surface of described plate lid, strongback is provided with between the plate lid of the Tissue Culture Plate of described pressure pad and top layer.
Further, described fixed mechanism comprises fixed block, and described fixed block is provided with internal thread, and described stationary shaft bottom is provided with the outside screw mated with the internal thread of described fixed block, and described fixed block is threaded with described stationary shaft.
Further, described fixed block outer edge surface is provided with cross swivel mount.
Further, described support is vertically placed with transverse axis fixed orifices, and the end of described transverse axis is supported in described transverse axis fixed orifices.
Further, described transverse axis end is provided with axial installation cavity, is provided with spring pin in described installation cavity, and described spring pin can along described transverse axis axial stretching.
Further, the outer edge surface of described transverse axis is provided with the stopper slot of installation cavity described in UNICOM, and the shank of described spring pin is provided with shifting block, and described shifting block passes described stopper slot and exposes to the outer edge surface of described transverse axis.
Further, between adjacent described support, described base plate is provided with the groove mated with described Tissue Culture Plate.
Further, the bottom of described supporter is provided with linker, and described linker runs through described plate lid, and in gap between the adjacent culture hole that can be plugged in described orifice plate.
Further, be respectively equipped with the locating slot coordinated with described supporter on the lower surface of the orifice plate of described Tissue Culture Plate and on the lower surface of described strongback, the top of described supporter is plugged in described locating slot.
Further, the bottom of described linker is arranged with gum cover.
After adopting structure of the present invention, its beneficial effect is: some Tissue Culture Plates are stacked on base plate, and compressed by the pressure pad of stationary shaft lower end, stationary shaft can vertically be gone up, lower movement, and fixed by fixed mechanism, the upper surface of the plate lid of Tissue Culture Plate is provided with supporter, the locating slot coordinated with described supporter is respectively equipped with on the lower surface of orifice plate and on the lower surface of strongback, the top of described supporter is plugged in described locating slot, it effectively can compress the multiple Tissue Culture Plates after stacking, and make to be provided with space between adjacent Tissue Culture Plate and between the Tissue Culture Plate of top layer and strongback, guarantee the mobility of air, the plate lid of Tissue Culture Plate has good gaseous interchange effect with extraneous, ensure that under same culture condition, carry out the screening of more planks simultaneously, improve the utilization ratio in shaking table space and the screening efficiency of cell, fixed mechanism comprises fixed block, fixed block is provided with internal thread, stationary shaft bottom is provided with the outside screw mated with the internal thread of fixed block, fixed block is threaded with stationary shaft, and support is vertically placed with transverse axis fixed orifices, and the end of transverse axis is supported in transverse axis fixed orifices, the increase of Tissue Culture Plate and convenient to remove, fast, only need move up and down the position of transverse axis on support, rotate cross rotary fixing frame and compress.
Accompanying drawing explanation
Fig. 1 is the structural representation of one embodiment of the invention;
Fig. 2 is the structural representation in Fig. 1 after A place partial enlargement;
Fig. 3 is the plan structure schematic diagram of one embodiment of the invention.
Embodiment
As shown in Figure 1 to Figure 3, comprise base plate 1, some Tissue Culture Plates stack and are placed on base plate 1, base plate 1 is provided with hold-down mechanism, hold-down mechanism comprises transverse axis 3, support 2, stationary shaft 5 and axle sleeve 4, transverse axis 3 is set in parallel in above Tissue Culture Plate, its two ends are supported by a pack support 2, support 2 is fixed on base plate 1, transverse axis 3 is provided with axle sleeve 4, stationary shaft 5 is installed on axle sleeve 4, and can vertically go up, lower movement, the lower end of stationary shaft 5 is provided with pressure pad 17, also comprise restriction stationary shaft 5 vertically to go up, the fixed mechanism of lower movement, the plate lid 13 of Tissue Culture Plate is provided with supporter 14, supporter 14 is at least provided with 3, and all with the upper surface being distributed in plate lid 13, the upper surface of supporter 14 is plane, and be parallel to the upper surface of plate lid 13, strongback 16 is provided with between the plate lid 13 of the Tissue Culture Plate of pressure pad 17 and top layer, the locating slot 18 coordinated with supporter is respectively equipped with on the lower surface of the orifice plate 15 of Tissue Culture Plate and on the lower surface of strongback 16, the top of supporter 14 is plugged in locating slot 18, fixed mechanism comprises fixed block, and fixed block is provided with internal thread, and stationary shaft 5 bottom is provided with the outside screw mated with the internal thread of fixed block, and fixed block is threaded with stationary shaft 5, fixed block outer edge surface is provided with cross swivel mount 12, support 2 is vertically placed with transverse axis fixed orifices 11, and the end of transverse axis 3 is supported in transverse axis fixed orifices 11, transverse axis 3 end is provided with axial installation cavity 9, is provided with spring pin in installation cavity 9, and spring pin comprises shank 6 and spring 8, and spring is against the bottom face of shank 6 and installation cavity 9, and shank 6 can under the action of the spring can along transverse axis 3 axial stretching, the outer edge surface of transverse axis 3 is provided with the stopper slot 10 of UNICOM's installation cavity 9, and the shank of spring pin is provided with shifting block 7, and shifting block 7 passes stopper slot 10 and exposes to the outer edge surface of transverse axis 3, the bottom of supporter 14 is provided with linker, and in the gap between linker is plugged in orifice plate adjacent culture hole after running through cover plate 13, the bottom of linker is arranged with gum cover, and it effectively can improve the steadiness that plate lid is connected with orifice plate.
This hold-down mechanism is applicable to the cultivation of the various kinds of cell types such as microorganism, mammalian cell, vegetable cell, insect cell, meanwhile, is also applicable to the cell cultures mode that this two class of suspension culture and adherent culture is different.
Concrete operating process is as follows:
First rotary cross swivel mount, unclamps fixed block, inwardly pulls shifting block simultaneously, shank is inwardly shunk, moves up and down transverse axis, until move to suitable fixed orifices, unclamp shifting block, shank front end is plugged in fixed orifices, thus fixing transverse axis.Successively by adding substratum, inoculated culture plate is stacked in the groove of base plate, place appropriate after, strongback is put on the superiors' culture plate, strongback plays location and is fixed the effect of culture plate, screw cross swivel mount, make pressure pad compress strongback, base plate is put into shaking table, and be secured by bolts in shaking table plate face, fix rear operation shaking table and cultivate.One group of press mounting structure can fix polylith Tissue Culture Plate simultaneously, and can arrange position or the quantity of the fixed orifices on support according to the quantity of Tissue Culture Plate.
Claims (10)
1. the press mounting structure of multi-layer cellular culture plate, comprise base plate, some Tissue Culture Plates stack and are placed on described base plate, described base plate is provided with hold-down mechanism, described hold-down mechanism comprises transverse axis, support, stationary shaft and axle sleeve, described transverse axis is set in parallel in above Tissue Culture Plate, its two ends are supported by a pack support, described support is fixed on base plate, described transverse axis is provided with axle sleeve, described stationary shaft is installed on described axle sleeve, and can vertically go up, lower movement, the lower end of described stationary shaft is provided with pressure pad, also comprise the described stationary shaft of restriction vertically to go up, the fixed mechanism of lower movement, the plate of described Tissue Culture Plate covers and is provided with supporter, the upper surface of described supporter is plane, and be parallel to the upper surface of described plate lid, strongback is provided with between the plate lid of the Tissue Culture Plate of described pressure pad and top layer.
2. the press mounting structure of multi-layer cellular culture plate according to claim 1, it is characterized in that: described fixed mechanism comprises fixed block, described fixed block is provided with internal thread, described stationary shaft bottom is provided with the outside screw mated with the internal thread of described fixed block, and described fixed block is threaded with described stationary shaft.
3. the press mounting structure of multi-layer cellular culture plate according to claim 2, is characterized in that: described fixed block outer edge surface is provided with cross swivel mount.
4. the press mounting structure of multi-layer cellular culture plate according to claim 2, is characterized in that: described support is vertically placed with transverse axis fixed orifices, and the end of described transverse axis is supported in described transverse axis fixed orifices.
5. the press mounting structure of multi-layer cellular culture plate according to claim 4, is characterized in that: described transverse axis end is provided with axial installation cavity, is provided with spring pin in described installation cavity, and described spring pin can along described transverse axis axial stretching.
6. the press mounting structure of multi-layer cellular culture plate according to claim 5, it is characterized in that: the outer edge surface of described transverse axis is provided with the stopper slot of installation cavity described in UNICOM, the shank of described spring pin is provided with shifting block, and described shifting block passes described stopper slot and exposes to the outer edge surface of described transverse axis.
7. the press mounting structure of multi-layer cellular culture plate according to claim 1, is characterized in that: between adjacent described support, and described base plate is provided with the groove mated with described Tissue Culture Plate.
8. according to the press mounting structure of described multi-layer cellular culture plate arbitrary in claim 1 to 7, it is characterized in that: the bottom of described supporter is provided with linker, described linker runs through described plate lid, and in gap between the adjacent culture hole that can be plugged in described orifice plate.
9. the press mounting structure of multi-layer cellular culture plate according to claim 8, it is characterized in that: be respectively equipped with the locating slot coordinated with described supporter on the lower surface of the orifice plate of described Tissue Culture Plate and on the lower surface of described strongback, the top of described supporter is plugged in described locating slot.
10. the press mounting structure of multi-layer cellular culture plate according to claim 9, is characterized in that: the bottom of described linker is arranged with gum cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744648.XA CN104371921B (en) | 2014-12-09 | 2014-12-09 | The press-loading apparatus of multi-layer cellular culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744648.XA CN104371921B (en) | 2014-12-09 | 2014-12-09 | The press-loading apparatus of multi-layer cellular culture plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104371921A true CN104371921A (en) | 2015-02-25 |
| CN104371921B CN104371921B (en) | 2017-04-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410744648.XA Active CN104371921B (en) | 2014-12-09 | 2014-12-09 | The press-loading apparatus of multi-layer cellular culture plate |
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| CN (1) | CN104371921B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619787A (en) * | 2017-11-10 | 2018-01-23 | 安徽金思源生物科技有限公司 | Constant temperature shaking table for biological laboratory |
| CN114574317A (en) * | 2022-01-25 | 2022-06-03 | 长春中医药大学 | Press mounting device for multilayer cell culture plate |
Citations (6)
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| CN2167163Y (en) * | 1993-06-01 | 1994-06-01 | 雷启元 | Spring lock bolt |
| CN1477936A (en) * | 2000-10-17 | 2004-02-25 | �����塤������ŵ | Fixing and/or assembling device |
| CN201080478Y (en) * | 2007-07-03 | 2008-07-02 | 海尔集团公司 | Gene magnification instrument thermal cap compressing apparatus |
| CN201459104U (en) * | 2009-03-05 | 2010-05-12 | 华东理工大学 | Micro-porous plate fixing device |
| CN103013829A (en) * | 2012-12-28 | 2013-04-03 | 江南大学 | Structure of deep-well cell culture plate cover |
| CN204325374U (en) * | 2014-12-09 | 2015-05-13 | 江南大学 | A kind of press mounting structure of multi-layer cellular culture plate |
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2014
- 2014-12-09 CN CN201410744648.XA patent/CN104371921B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2167163Y (en) * | 1993-06-01 | 1994-06-01 | 雷启元 | Spring lock bolt |
| CN1477936A (en) * | 2000-10-17 | 2004-02-25 | �����塤������ŵ | Fixing and/or assembling device |
| CN201080478Y (en) * | 2007-07-03 | 2008-07-02 | 海尔集团公司 | Gene magnification instrument thermal cap compressing apparatus |
| CN201459104U (en) * | 2009-03-05 | 2010-05-12 | 华东理工大学 | Micro-porous plate fixing device |
| CN103013829A (en) * | 2012-12-28 | 2013-04-03 | 江南大学 | Structure of deep-well cell culture plate cover |
| CN204325374U (en) * | 2014-12-09 | 2015-05-13 | 江南大学 | A kind of press mounting structure of multi-layer cellular culture plate |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619787A (en) * | 2017-11-10 | 2018-01-23 | 安徽金思源生物科技有限公司 | Constant temperature shaking table for biological laboratory |
| CN114574317A (en) * | 2022-01-25 | 2022-06-03 | 长春中医药大学 | Press mounting device for multilayer cell culture plate |
| CN114574317B (en) * | 2022-01-25 | 2024-04-09 | 长春中医药大学 | Press mounting device for multilayer cell culture plates |
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| Publication number | Publication date |
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| CN104371921B (en) | 2017-04-05 |
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