CN104371921B - The press-loading apparatus of multi-layer cellular culture plate - Google Patents
The press-loading apparatus of multi-layer cellular culture plate Download PDFInfo
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- CN104371921B CN104371921B CN201410744648.XA CN201410744648A CN104371921B CN 104371921 B CN104371921 B CN 104371921B CN 201410744648 A CN201410744648 A CN 201410744648A CN 104371921 B CN104371921 B CN 104371921B
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- plate
- culture plate
- transverse axis
- tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention provides the press mounting structure of multi-layer cellular culture plate, its multiple Tissue Culture Plate after stacking is fixed in wanting on bed, it is ensured that effectively make use of the vertical blank of shaking table while shallow bore hole Tissue Culture Plate plate lid is with culture plate body seal and air flow property again.Which includes base plate, some Tissue Culture Plates are stacked and are placed on base plate, base plate is provided with hold-down mechanism, hold-down mechanism includes transverse axis, support, fixing axle and axle sleeve, transverse axis is set in parallel in above Tissue Culture Plate, its two ends is supported by a pack support, support is fixed on base plate, transverse axis is provided with axle sleeve, fixing axle is installed on axle sleeve and vertically can move up and down, the lower end of fixing axle is provided with pressure pad, also include limiting the fixed mechanism that fixing axle is vertically moved up and down, the plate lid of Tissue Culture Plate is provided with supporter.
Description
Technical field
The present invention relates to the fixing device of Tissue Culture Plate, and in particular to the press-loading apparatus of multi-layer cellular culture plate.
Background technology
With the development of molecular biology, the heterogenous expression technology of albumen is gradually ripe and is widely used.Foreign protein
Expression need to select suitable expressive host and Expression element, this is accomplished by building huge expression library, therefrom selects
Preferably representation aids box.Tissue Culture Plate can carry out high-flux cell culture and strain, cell using less culture fluid
Strain and expression library screening, with quick, high flux, it is cost-effective the features such as.
Existing Tissue Culture Plate needs to seal plate mouth when in use in order to avoid cross-contamination between microbiological contamination or plate hole,
Now mainly there are two kinds of sealing plate mouth modes, a kind of is that latex mattress is sealed, it is a kind of for " sandwich " formula plate cover seal, " sandwich "
Formula plate lid can carry out gas exchange well, it is to avoid cross-contamination.But sealing cannot when plate lid and orifice plate are used in combination
Ensure, and need specific culture shaking table.And orifice plate size of shaking table plate face in shaking table culture has been limited to can be while train
Foster orifice plate number.And shaking table solid space can not be utilized effectively.
The content of the invention
For the problems referred to above, the invention provides the press-loading apparatus of multi-layer cellular culture plate, its will can stack after it is multiple
Tissue Culture Plate is fixed in wanting on bed, it is ensured that shallow bore hole Tissue Culture Plate plate lid and culture plate body seal and air flow
Property while again effectively make use of the vertical space of shaking table.
Its technical scheme is such, and including base plate, some Tissue Culture Plates are stacked and are placed on the base plate, the bottom
Plate is provided with hold-down mechanism, and the hold-down mechanism includes transverse axis, support, fixing axle and axle sleeve, and the transverse axis is set in parallel in carefully
Above born of the same parents' culture plate, its two ends supported by a pack support, the support is fixed on base plate, and the transverse axis is provided with axle sleeve,
The fixing axle is installed on the axle sleeve and vertically can move up and down, and the lower end of the fixing axle is provided with pressure pad,
Also include limiting the fixed mechanism that the fixing axle is vertically moved up and down, the plate lid of the Tissue Culture Plate is provided with
Supporter, the upper surface of the supporter are the cell of plane the upper surface parallel to the plate lid, the pressure pad and top layer
Location-plate is provided between the plate lid of culture plate.
Further, the fixed mechanism includes fixed block, and the fixed block is provided with female thread, and the fixing axle bottom sets
There is the external screw thread matched with the female thread of the fixed block, the fixed block is threadedly coupled with the fixing axle.
Further, the fixed block outer edge surface is provided with cross swivel mount.
Further, the support is vertically placed with transverse axis fixing hole, and the end of the transverse axis is supported in described
In transverse axis fixing hole.
Further, the transverse axis end is provided with axial installation cavity, and the installation intracavity is provided with resilient key, the elasticity
Pin can be along the transverse axis axial stretching.
Further, the outer edge surface of the transverse axis is provided with the stopper slot of installation cavity described in UNICOM, the pin of the resilient key
Body is provided with shifting block, and the shifting block passes through the stopper slot and exposes to the outer edge surface of the transverse axis.
Further, the base plate is provided with the groove matched with the Tissue Culture Plate, and the groove is located at adjacent institute
State between support.
Further, the bottom of the supporter is provided with connector, and the connector runs through the plate lid, and can insert
In gap between the adjacent culture hole of the orifice plate.
Further, it is respectively equipped with the lower surface of the orifice plate of the Tissue Culture Plate and on the lower surface of the location-plate
The locating slot coordinated with the supporter, the top of the supporter is plugged in the locating slot.
Further, the bottom of the connector is arranged with gum cover.
After structure using the present invention, its advantage is：Some Tissue Culture Plates are stacked on base plate, and by fixing
The pressure pad of axle lower end is compressed, and fixing axle vertically can be moved up and down, and be fixed by fixed mechanism, Tissue Culture Plate
The upper surface of plate lid is provided with supporter, is respectively equipped with and the supporter on the lower surface of orifice plate and on the lower surface of location-plate
The locating slot of cooperation, the top of the supporter are plugged in the locating slot, and which can effectively compress the multiple cells after stacking
Culture plate, and make to be provided with space between adjacent Tissue Culture Plate and between the Tissue Culture Plate and location-plate of top layer, it is ensured that
The mobility of air, the plate lid of Tissue Culture Plate have good gas exchange effect with extraneous, it is ensured that in same culture bar
Under part, while carrying out the screening of more planks, the screening efficiency of the utilization rate and cell in shaking table space is improved；Fixed mechanism bag
Include fixed block, fixed block is provided with female thread, fixing axle bottom is provided with the external screw thread matched with the female thread of fixed block, fixed block with
Fixing axle is threadedly coupled, and support is vertically placed with transverse axis fixing hole, and the end of transverse axis is supported in transverse axis fixing hole, carefully
The increase of born of the same parents' culture plate and convenient to remove, quickly, need to only move up and down position of the transverse axis on support, rotate cross rotation and fix
Frame is compressed.
Description of the drawings
Structural representations of the Fig. 1 for one embodiment of the invention；
Fig. 2 is the structural representation after partial enlargement at A in Fig. 1；
Overlooking the structure diagrams of the Fig. 3 for one embodiment of the invention.
Specific embodiment
As shown in Figure 1 to Figure 3, including base plate 1, some Tissue Culture Plates are stacked and are placed on base plate 1, and base plate 1 is provided with
Hold-down mechanism, hold-down mechanism include transverse axis 3, support 2, fixing axle 5 and axle sleeve 4, and transverse axis 3 is set in parallel on Tissue Culture Plate
Side, its two ends are supported by a pack support 2, and support 2 is fixed on base plate 1, and transverse axis 3 is provided with axle sleeve 4, and fixing axle 5 is installed on
Axle sleeve 4, and vertically can move up and down, the lower end of fixing axle 5 is provided with pressure pad 17, also including limiting fixing axle 5 along perpendicular
The fixed mechanism that Nogata is moved up and down, the plate lid 13 of Tissue Culture Plate are provided with supporter 14, and supporter 14 is at least provided with 3
It is individual, and with the upper surface for being distributed in plate lid 13, the upper surface of supporter 14 is plane the upper surface parallel to plate lid 13,
Location-plate 16 is provided between the plate lid 13 of the Tissue Culture Plate of pressure pad 17 and top layer, on the lower surface of the orifice plate 15 of Tissue Culture Plate
Be respectively equipped with the lower surface of location-plate 16 with supporter coordinate locating slot 18, the top of supporter 14 is plugged in locating slot
In 18；Fixed mechanism includes fixed block, and fixed block is provided with female thread, and 5 bottom of fixing axle is provided with and is matched with the female thread of fixed block
External screw thread, fixed block is threadedly coupled with fixing axle 5；Fixed block outer edge surface is provided with cross swivel mount 12；Support 2 is along vertical side
To transverse axis fixing hole 11 is placed with, the end of transverse axis 3 is supported in transverse axis fixing hole 11；3 end of transverse axis is provided with axial installation
Chamber 9, is provided with resilient key in installation cavity 9, and resilient key includes shank 6 and spring 8, and spring is against shank 6 and the bottom of installation cavity 9
Face, shank 6 can under the action of the spring can be along 3 axial stretching of transverse axis；The outer edge surface of transverse axis 3 is provided with UNICOM's installation cavity 9
Stopper slot 10, the shank of resilient key is provided with shifting block 7, shifting block 7 through stopper slot 10, and expose to the outer edge surface of transverse axis 3；
The bottom of support body 14 is provided with connector, and connector is plugged in the gap between the adjacent culture hole of orifice plate after cover plate 13,
The bottom of connector is arranged with gum cover, and which can effectively improve the steadiness of plate lid and orifice plate connection.
The hold-down mechanism is applied to the training of the various kinds of cell types such as microorganism, mammalian cell, plant cell, insect cell
Support, meanwhile, it is also applied for the different cell culture mode of suspension culture and adhere-wall culture this two class.
Specific operating process is as follows：
Rotary cross swivel mount, unclamps fixed block, while inwardly pulling shifting block, makes shank to contract, upper and lower shifting first
Dynamic transverse axis, until moving to suitable fixing hole, unclamps shifting block, shank front end is plugged in fixing hole, so as to fixed transverse axis.According to
The secondary culture medium, inoculated culture plate of adding is stacked in the groove of base plate, after placement is appropriate, is put on the superiors' culture plate
Enter location-plate, location-plate plays a part of to position and is fixed culture plate, screws cross swivel mount, makes pressure pad compress positioning
Plate, base plate is put in shaking table, and is secured by bolts in shaking table plate face, runs shaking table and cultivated after fixing.One group of pressure
Assembling device can fix polylith Tissue Culture Plate simultaneously, and can be according to the position of the fixing hole on the quantity of Tissue Culture Plate setting support
Put or quantity.
Claims (8)
1. the press-loading apparatus of multi-layer cellular culture plate, including base plate, some Tissue Culture Plates are stacked and are placed on the base plate, institute
State base plate and be provided with hold-down mechanism, the hold-down mechanism includes transverse axis, support, fixing axle and axle sleeve, and the transverse axis be arranged in parallel
Above Tissue Culture Plate, its two ends supported by a pack support, the support is fixed on base plate, and the transverse axis is provided with axle
Set, the fixing axle are installed on the axle sleeve and vertically can move up and down, and the lower end of the fixing axle is provided with pressure
Pad, also including the fixed mechanism that the fixing axle is vertically moved up and down is limited, sets on the plate lid of the Tissue Culture Plate
There is a supporter, the upper surface of the supporter is plane the upper surface parallel to the plate lid, the pressure pad is thin with top layer
Location-plate is provided between the plate lid of born of the same parents' culture plate；The fixed mechanism includes fixed block, and the fixed block is provided with female thread, described
Fixing axle bottom is provided with the external screw thread matched with the female thread of the fixed block, and the fixed block is connected with the fixed axle thread
Connect；The support is vertically placed with transverse axis fixing hole, and the end of the transverse axis is supported in the transverse axis fixing hole.
2. press-loading apparatus of multi-layer cellular culture plate according to claim 1, it is characterised in that：The fixed block outer edge surface
It is provided with cross swivel mount.
3. press-loading apparatus of multi-layer cellular culture plate according to claim 1, it is characterised in that：The transverse axis end is provided with
The installation cavity of axial direction, the installation intracavity are provided with resilient key, and the resilient key can be along the transverse axis axial stretching.
4. press-loading apparatus of multi-layer cellular culture plate according to claim 3, it is characterised in that：The outer edge surface of the transverse axis
The stopper slot of installation cavity described in UNICOM is provided with, the shank of the resilient key is provided with shifting block, and the shifting block passes through described spacing
Groove simultaneously exposes to the outer edge surface of the transverse axis.
5. press-loading apparatus of multi-layer cellular culture plate according to claim 1, it is characterised in that：The base plate be provided with
The groove of the Tissue Culture Plate matching, the groove are located between the adjacent support.
6. press-loading apparatus according to arbitrary described multi-layer cellular culture plate in claim 1 to 5, it is characterised in that：Described
The bottom of support body is provided with connector, and the connector runs through the plate lid, and can be plugged in the adjacent culture hole of the orifice plate
Between gap in.
7. press-loading apparatus of multi-layer cellular culture plate according to claim 6, it is characterised in that：The Tissue Culture Plate
The locating slot coordinated with the supporter, the support are respectively equipped with the lower surface of orifice plate and on the lower surface of the location-plate
The top of body is plugged in the locating slot.
8. press-loading apparatus of multi-layer cellular culture plate according to claim 7, it is characterised in that：The bottom of the connector
It is arranged with gum cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744648.XA CN104371921B (en) | 2014-12-09 | 2014-12-09 | The press-loading apparatus of multi-layer cellular culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744648.XA CN104371921B (en) | 2014-12-09 | 2014-12-09 | The press-loading apparatus of multi-layer cellular culture plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104371921A CN104371921A (en) | 2015-02-25 |
| CN104371921B true CN104371921B (en) | 2017-04-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410744648.XA Active CN104371921B (en) | 2014-12-09 | 2014-12-09 | The press-loading apparatus of multi-layer cellular culture plate |
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| CN (1) | CN104371921B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619787B (en) * | 2017-11-10 | 2020-12-22 | 安徽金思源生物科技有限公司 | Constant temperature shaking table for biological laboratory |
| CN114574317B (en) * | 2022-01-25 | 2024-04-09 | 长春中医药大学 | Press mounting device for multilayer cell culture plates |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2167163Y (en) * | 1993-06-01 | 1994-06-01 | 雷启元 | Spring lock bolt |
| JP2004512475A (en) * | 2000-10-17 | 2004-04-22 | ジョルダーノ，ジュゼッペ | Fixing and / or assembly tools |
| CN201080478Y (en) * | 2007-07-03 | 2008-07-02 | 海尔集团公司 | Gene magnification instrument thermal cap compressing apparatus |
| CN201459104U (en) * | 2009-03-05 | 2010-05-12 | 华东理工大学 | Micro-porous plate fixing device |
| CN103013829B (en) * | 2012-12-28 | 2015-04-22 | 江南大学 | Structure of deep-well cell culture plate cover |
| CN204325374U (en) * | 2014-12-09 | 2015-05-13 | 江南大学 | A kind of press mounting structure of multi-layer cellular culture plate |
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| Publication number | Publication date |
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| CN104371921A (en) | 2015-02-25 |
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