CN204918603U - Cell co -culture system under pressure environment - Google Patents
Cell co -culture system under pressure environment Download PDFInfo
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- CN204918603U CN204918603U CN201520546197.9U CN201520546197U CN204918603U CN 204918603 U CN204918603 U CN 204918603U CN 201520546197 U CN201520546197 U CN 201520546197U CN 204918603 U CN204918603 U CN 204918603U
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Abstract
本实用新型公开了一种压力环境下细胞共培养系统，包括压力仓、高压气瓶、金属管、培养皿和共培养小室；密封压力仓通过金属管与高压气瓶连通；压力仓内设置有培养皿，培养皿中设有共培养小室，两种细胞分别放置在培养皿和共培养小室内；培养皿内设置有培养液，两种细胞通过培养液接触。该装置结构简单，操作方便，并且可根据具体实验背景调节压力环境大小和细胞比例。
The utility model discloses a cell co-cultivation system under pressure environment, which comprises a pressure chamber, a high-pressure gas cylinder, a metal tube, a culture dish and a co-cultivation chamber; the sealed pressure chamber communicates with the high-pressure gas cylinder through the metal pipe; the pressure chamber is provided with The petri dish is provided with a co-cultivation chamber, and two kinds of cells are respectively placed in the petri dish and the co-cultivation chamber; the culture dish is provided with a culture solution, and the two kinds of cells are in contact with each other through the culture solution. The device has a simple structure and is convenient to operate, and the size of the pressure environment and the cell ratio can be adjusted according to the specific experimental background.
Description
[technical field]
The utility model relates to cell biological culture technique field, particularly co-culture of cells system under a kind of pressure environment.
[background technology]
At present, in cytobiology correlative study, the correlative study of cultivating under pressure environment about cell more and more receives people's concern in biomechanics field.Its relative unit and system more, it mostly is and connects the mode of high pressure gas by closed metal storehouse and realize, and this system can meet the general cultivation of single class cell in hyperbaric environment.Because biomass cells has diversity, it is closely connected by various molecular pathways each other, influences each other, and therefore, co-culture system is the important means studying biological cell function.But the High pressure culture device existed at present can not meet the research of various kinds of cell contact and impact in hyperbaric environment is cultivated.In view of above limitation, this device is intended to set up and a set ofly meets two kinds of cells carry out high pressure Dual culture system by the mode of noncontact Dual culture, it is easy and simple to handle, simple structure, successfully can set up hyperbaric environment, and study the change of two kinds of cells, be the further favourable condition that biomechanics correlative study is created.
At present, more for the device cultivated under cell hyperbaric environment both at home and abroad, but there are problems.Wherein, for the Dual culture still Shortcomings of various kinds of cell under hyperbaric environment.
[utility model content]
The utility model is in order to solve the High pressure culture problem under various kinds of cell Dual culture, a kind of simple and easy to do co-culture of cells pressure system is provided, this apparatus structure is simple, easy to operate, and can regulate pressure environment size and cell proportion according to specific experiment background.
The utility model is achieved through the following technical solutions:
Co-culture of cells system under a kind of pressure environment, comprises Pressure chamber, gas cylinder, metal tube, culture dish and Dual culture cell; Sealing load storehouse is communicated with gas cylinder by metal tube; Be provided with culture dish in Pressure chamber, be provided with Dual culture cell in culture dish, two kinds of cells are placed on culture dish and the little indoor of Dual culture respectively; Be provided with nutrient solution in culture dish, two kinds of cells are contacted by nutrient solution.
Described Pressure chamber is cylindrical, and the Pressure chamber upper cover sealed by steel base and steel forms, fixing by screw sealing between the two.
Described Dual culture cell is Traswell cell.
Gap is left between the bottom of described Dual culture cell and culture dish.
The liquid level of described nutrient solution floods Dual culture cell inner cell.
The pressure adjustable of described gas cylinder, air bottle is provided with tensimeter.
The external diameter diameter 300mm of described Pressure chamber, internal diameter 200mm.
The long 500mm of described metal tube, external diameter 6mm, internal diameter 3mm.
Compared with prior art, the utility model has following useful technique effect:
Co-culture of cells system under a kind of pressure environment of the utility model, form primarily of Pressure chamber, gas cylinder, metal tube, culture dish and Dual culture cell, system component is less, compact construction, reasonable in design, easy to operate simple and easy, the noncontact Dual culture of two kinds of cells can be carried out under hyperbaric environment, for biomechanics related experiment provides favourable condition.Native system can be applicable in the pressure Dual culture of stem cell and nucleus pulposus cell, and obtains good result.
[accompanying drawing explanation]
Fig. 1 is co-culture of cells system schematic under pressure environment;
Fig. 2 is the main pseudosection of co-culture of cells under pressure environment.
Wherein: 1, Pressure chamber upper cover; 2, Pressure chamber; 3, Dual culture cell; 4, culture dish; 5, metal tube; 6 gas cylinders; 7, tensimeter; 8, nutrient solution; 9, cell A; 10, cell B.
[embodiment]
Below in conjunction with accompanying drawing, the utility model is described in further detail:
As depicted in figs. 1 and 2, co-culture of cells system under a kind of pressure environment of the utility model, mainly comprises Pressure chamber 2, gas cylinder 6, metal tube 5, culture dish 4, Dual culture cell 3 (i.e. Traswell cell).Pressure chamber 2 is cylindrical, is made up of the Pressure chamber upper cover 1 of steel base and steel sealing cover, has four pieces of screw sealings fixing between the two.Its top connection metal pipe 5, is connected with gas cylinder 6.Culture dish holding 4 in Pressure chamber 2, has Dual culture cell 3 in culture dish 4.When carrying out Dual culture, two kinds of cells being placed in culture dish 4 and Dual culture cell 3 respectively, being contacted by nutrient solution 8 between the two, confining pressure storehouse 2, regulate gas cylinder 6 pressure to regulate the pressure of Dual culture environment.
As shown in Figure 2, when native system specifically makes and uses, adopt cylindrical steel Pressure chamber 2 (external diameter diameter 300mm, internal diameter 200mm), storehouse is built-in with culture dish 4, culture dish 4 is built-in with Dual culture cell 3, two kinds of cell A and B is placed in respectively culture dish 4 and cultivates cell 3, nutrient solution 8 of annotating in culture dish 4.Pressure chamber upper cover is sealed in Pressure chamber, and with metal tube 5 (long 500mm, external diameter 6mm internal diameter 3mm) Bonding pressure storehouse and gas cylinder 6.Regulate gas cylinder 6 pressure afterwards, by tensimeter 7 adjustable pressure to regulate Pressure chamber 2 internal pressure, carry out the research of associated biomolecule cyto-mechanics.
Above content is in conjunction with concrete preferred implementation further detailed description of the utility model; can not assert that embodiment of the present utility model is only limitted to this; for the utility model person of an ordinary skill in the technical field; under the prerequisite of not isolating the utility model design; some simple deduction or replace can also be made, all should be considered as belonging to the utility model by submitted to claims determination scope of patent protection.
Claims (8)
1. a co-culture of cells system under pressure environment, is characterized in that, comprises Pressure chamber (2), gas cylinder (6), metal tube (5), culture dish (4) and Dual culture cell (3); Sealing load storehouse (2) is communicated with gas cylinder (6) by metal tube (5); Be provided with culture dish (4) in Pressure chamber (2), be provided with Dual culture cell (3) in culture dish (4), two kinds of cells are placed in culture dish (4) and Dual culture cell (3) respectively; Be provided with nutrient solution (8) in culture dish (4), two kinds of cells are by nutrient solution (8) contact.
2. co-culture of cells system under a kind of pressure environment according to claim 1, it is characterized in that, described Pressure chamber (2) is cylindrical, and the Pressure chamber upper cover (1) sealed by steel base and steel forms, fixing by screw sealing between the two.
3. co-culture of cells system under a kind of pressure environment according to claim 1, is characterized in that, described Dual culture cell (3) is Traswell cell.
4. co-culture of cells system under a kind of pressure environment according to claim 1, is characterized in that, leaves gap between the bottom of described Dual culture cell (3) and culture dish (4).
5. co-culture of cells system under a kind of pressure environment according to claim 1, is characterized in that, the liquid level of described nutrient solution (8) floods Dual culture cell (3) inner cell.
6. co-culture of cells system under a kind of pressure environment according to claim 1, is characterized in that, the pressure adjustable of described gas cylinder (6), air bottle (6) is provided with tensimeter (7).
7. co-culture of cells system under a kind of pressure environment according to claim 1, is characterized in that, the external diameter diameter 300mm of described Pressure chamber (2), internal diameter 200mm.
8. co-culture of cells system under a kind of pressure environment according to claim 1, is characterized in that, the long 500mm of described metal tube (5), external diameter 6mm, internal diameter 3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520546197.9U CN204918603U (en) | 2015-07-24 | 2015-07-24 | Cell co -culture system under pressure environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520546197.9U CN204918603U (en) | 2015-07-24 | 2015-07-24 | Cell co -culture system under pressure environment |
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| Publication Number | Publication Date |
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| CN204918603U true CN204918603U (en) | 2015-12-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520546197.9U Expired - Fee Related CN204918603U (en) | 2015-07-24 | 2015-07-24 | Cell co -culture system under pressure environment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728360A (en) * | 2018-06-09 | 2018-11-02 | 上海天引生物科技有限公司 | A kind of devices and methods therefor that cell co-cultures |
| CN109991403A (en) * | 2019-05-17 | 2019-07-09 | 王春晖 | A kind of experimental provision that establishing blast injury cell model and experimental method |
-
2015
- 2015-07-24 CN CN201520546197.9U patent/CN204918603U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728360A (en) * | 2018-06-09 | 2018-11-02 | 上海天引生物科技有限公司 | A kind of devices and methods therefor that cell co-cultures |
| CN108728360B (en) * | 2018-06-09 | 2024-04-09 | 上海天引生物科技有限公司 | Cell co-culture device and method thereof |
| CN109991403A (en) * | 2019-05-17 | 2019-07-09 | 王春晖 | A kind of experimental provision that establishing blast injury cell model and experimental method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151230 Termination date: 20190724 |