CN104371922B - The compression mechanism of multi-layer cellular culture plate - Google Patents
The compression mechanism of multi-layer cellular culture plate Download PDFInfo
- Publication number
- CN104371922B CN104371922B CN201410744815.0A CN201410744815A CN104371922B CN 104371922 B CN104371922 B CN 104371922B CN 201410744815 A CN201410744815 A CN 201410744815A CN 104371922 B CN104371922 B CN 104371922B
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- transverse axis
- stationary shaft
- strongback
- plate
- culture plate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
Abstract
The present invention provides the compression mechanism of multi-layer cellular culture plate, its energy pressure strip lid and orifice plate, it is ensured that stopping property, and can effectively compress the Tissue Culture Plate after multiple stacking, it is to increase the utilization ratio in shaking table space. It comprises transverse axis, support, stationary shaft and axle sleeve, transverse axis two ends are supported by a pack support, support is fixed on base plate, transverse axis is provided with axle sleeve, stationary shaft is installed on axle sleeve and can vertically move up and down, the lower end of stationary shaft is provided with pressure pad, also comprises the fixed mechanism that restriction stationary shaft vertically moves up and down.
Description
Technical field
The present invention relates to the stationary installation of Tissue Culture Plate, it is specifically related to the compression mechanism of multi-layer cellular culture plate.
Background technology
Along with molecular biological development, the heterogenous expression technology of albumen is ripe gradually and is widely used. The expression of foreign protein needs to select suitable expressive host and Expression element, and this just needs to build huge expression library, therefrom selects preferably representation aids box. Tissue Culture Plate can utilize less nutrient solution to carry out high-flux cell cultivation and the screening of bacterial classification, cell strain and expression library, has fast, high-throughput, the feature such as cost-saving.
Existing Tissue Culture Plate is in use in order to avoid crossed contamination between microbiological contamination or plate hole, need to be sealed by plate mouth, now mainly contain two kinds of sealing plate mouth modes, one is that latex pad is sealed, one is the sealing of " sandwich " formula plate lid, " sandwich " formula plate lid can well carry out gaseous interchange, avoids crossed contamination. But stopping property cannot ensure when plate lid and orifice plate are combined, and need specifically to cultivate shaking table. And the size in orifice plate shaking table plate face when shaking table is cultivated has limited to the orifice plate number that can cultivate simultaneously. And shaking table solid space can not be utilized effectively.
Summary of the invention
For the problems referred to above, the present invention provides the compression mechanism of multi-layer cellular culture plate, its energy pressure strip lid and orifice plate, it is ensured that stopping property, and can effectively compress the multiple Tissue Culture Plates after stacking, it is to increase the utilization ratio in shaking table space.
Its technical scheme is such, comprise transverse axis, support, stationary shaft and axle sleeve, described transverse axis two ends are supported by a pack support, described support is fixed on base plate, described transverse axis is provided with axle sleeve, described stationary shaft is installed on described axle sleeve and can vertically move up and down, and the lower end of described stationary shaft is provided with pressure pad, also comprises the fixed mechanism that the described stationary shaft of restriction vertically moves up and down.
Further, described fixed mechanism comprises fixed block, and described fixed block is provided with internal thread, and described stationary shaft bottom is provided with the outside screw that the internal thread with described fixed block mates, and described fixed block is threaded with described stationary shaft.
Further, described fixed block outer edge surface is provided with cross swivel mount.
Further, described support is vertically placed with transverse axis fixed orifices, and the end of described transverse axis is supported in described transverse axis fixed orifices.
Further, described transverse axis end is provided with axial installation chamber, is provided with spring pin in described installation chamber, and described spring pin can along described transverse axis axial stretching.
Further, the outer edge surface of described transverse axis is provided with the spacing groove installing chamber described in UNICOM, and the shank of described spring pin is provided with dials block, and described group of block passes described spacing groove and expose to the outer edge surface of described transverse axis.
Further, also comprise strongback, described strongback is arranged between the plate lid of the Tissue Culture Plate to be compressed of pressure pad and top layer, described strongback lower surface is provided with locating slot, the upper end of strut member is supported in locating slot, making to be provided with space between the plate lid of Tissue Culture Plate and strongback, described stationary shaft press-fits the upper surface of described strongback.
After adopting the structure of the present invention, its useful effect is: some Tissue Culture Plates are stacked between a pack support and compress by stationary shaft, stationary shaft can vertically move up and down, and fix by fixed mechanism, its energy pressure strip lid and orifice plate, it is ensured that stopping property, and can effectively compress the Tissue Culture Plate after multiple stacking, ensure that under same culture condition, carry out the screening of more planks simultaneously, it is to increase the utilization ratio in shaking table space and the screening efficiency of cell; Fixed mechanism comprises fixed block, fixed block is provided with internal thread, stationary shaft bottom is provided with the outside screw that the internal thread with fixed block mates, fixed block is threaded with stationary shaft, and support is vertically placed with transverse axis fixed orifices, and the end of transverse axis is supported in transverse axis fixed orifices, the increase of Tissue Culture Plate and convenient to remove, fast, only need to move up and down the position of transverse axis on support, rotate cross rotary fixing frame and compress.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention;
Fig. 2 is structural representation after A place partial enlargement in Fig. 1.
Embodiment
As shown in Figure 1 to Figure 2, comprise transverse axis 3, support 2, stationary shaft 5 and axle sleeve 4, transverse axis 3 two ends are supported by a pack support 2, support 2 is fixed on base plate 1, transverse axis 3 is provided with axle sleeve 4, stationary shaft 5 is installed on axle sleeve 4 and can vertically move up and down, and the lower end of stationary shaft 5 is provided with pressure pad 17, also comprises the fixed mechanism that restriction stationary shaft 5 vertically moves up and down, fixed mechanism comprises fixed block, and fixed block is provided with internal thread, and stationary shaft 5 bottom is provided with the outside screw that the internal thread with fixed block mates, and fixed block is threaded with stationary shaft 5, fixed block outer edge surface is provided with cross swivel mount 12, support 2 is vertically placed with transverse axis fixed orifices 11, and the end of transverse axis 3 is supported in transverse axis fixed orifices 11, transverse axis 3 end is provided with axial installation chamber 9, installs in chamber 9 and is provided with spring pin, and spring pin comprises shank 6 and spring 8, and spring is against shank 6 and the face, bottom installing chamber 9, and shank 6 can under the action of the spring can along transverse axis 3 axial stretching, the outer edge surface of transverse axis 3 is provided with the spacing groove 10 that chamber 9 is installed by UNICOM, and the shank of spring pin is provided with dials block 7, dials block 7 and passes spacing groove 10 and expose to the outer edge surface of transverse axis 3, also comprise strongback 16, some Tissue Culture Plates are stacked on base plate 1, and it is positioned at the underface of stationary shaft 5, the upper surface of the plate lid 13 of Tissue Culture Plate is provided with strut member 14, the lower surface of its orifice plate 15 is provided with locating slot, the upper end of strut member 14 is supported in locating slot, make to be provided with space between the plate lid 13 of downside Tissue Culture Plate and the orifice plate 15 of upside Tissue Culture Plate, the quantity of strut member and locating slot is five, it is distributed in culture plate lid and strongback corner and center, strongback is arranged between the plate lid of pressure pad and top layer Tissue Culture Plate to be compressed, strongback 16 lower surface is provided with locating slot, the plate that strongback is placed in Tissue Culture Plate covers, the upper end of strut member is supported in locating slot, make to be provided with space between the plate lid of Tissue Culture Plate and strongback, strongback 16 is arranged between the plate lid of pressure pad 17 and Tissue Culture Plate to be compressed, stationary shaft 5 press-fits the upper surface of strongback 16.
This compression mechanism is applicable to the cultivation of the various kinds of cell types such as microorganism, mammalian cell, vegetable cell, insect cell, meanwhile, is also applicable to the cell cultures mode that this two class of suspension culture and adherent culture is different.
Concrete operating process is as follows:
First rotary cross swivel mount, unclamps fixed block, inwardly pulls simultaneously and dials block, make shank to contract, move up and down transverse axis, until moving to suitable fixed orifices, unclamping and dialling block, shank front end is plugged in fixed orifices, thus fixing transverse axis. Successively by adding substratum, inoculated culture plate be stacked in the groove of base plate, this groove is between adjacent support, for proliferative rate plate, place appropriate after, on top layer culture plate, put into strongback, strongback plays location and is fixed the effect of culture plate, screw cross swivel mount, make pressure pad compress strongback, base plate is put into shaking table, and it is secured by bolts in shaking table plate face, run shaking table after fixing good and cultivate. One group of press mounting structure can fix polylith Tissue Culture Plate simultaneously, and can arrange position or the quantity of the fixed orifices on support according to the quantity of Tissue Culture Plate.
Claims (1)
1. the compression mechanism of multi-layer cellular culture plate, comprise transverse axis, support, stationary shaft and axle sleeve, described transverse axis two ends are supported by a pack support, described support is fixed on base plate, described transverse axis is provided with axle sleeve, described stationary shaft is installed on described axle sleeve and can vertically move up and down, and the lower end of described stationary shaft is provided with pressure pad, also comprises the fixed mechanism that the described stationary shaft of restriction vertically moves up and down; Described fixed mechanism comprises fixed block, and described fixed block is provided with internal thread, and described stationary shaft bottom is provided with the outside screw that the internal thread with described fixed block mates, and described fixed block is threaded with described stationary shaft; Described fixed block outer edge surface is provided with cross swivel mount; Described support is vertically placed with transverse axis fixed orifices, and the end of described transverse axis is supported in described transverse axis fixed orifices; Described transverse axis end is provided with axial installation chamber, is provided with spring pin in described installation chamber, and described spring pin can along described transverse axis axial stretching; The outer edge surface of described transverse axis is provided with the spacing groove being connected described installation chamber, and the shank of described spring pin is provided with dials block, and described group of block passes described spacing groove and expose to the outer edge surface of described transverse axis; Also comprise strongback, described strongback lower surface is provided with locating slot, described strongback is arranged between the plate lid of pressure pad and top layer Tissue Culture Plate to be compressed, the upper end of strut member is supported in locating slot and makes to be provided with space between the plate lid of Tissue Culture Plate and strongback, and described stationary shaft press-fits the upper surface of described strongback.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744815.0A CN104371922B (en) | 2014-12-09 | 2014-12-09 | The compression mechanism of multi-layer cellular culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744815.0A CN104371922B (en) | 2014-12-09 | 2014-12-09 | The compression mechanism of multi-layer cellular culture plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104371922A CN104371922A (en) | 2015-02-25 |
| CN104371922B true CN104371922B (en) | 2016-06-01 |
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| CN201410744815.0A Active CN104371922B (en) | 2014-12-09 | 2014-12-09 | The compression mechanism of multi-layer cellular culture plate |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114574317B (en) * | 2022-01-25 | 2024-04-09 | 长春中医药大学 | Press mounting device for multilayer cell culture plates |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201459104U (en) * | 2009-03-05 | 2010-05-12 | 华东理工大学 | Micro-porous plate fixing device |
| CN203904328U (en) * | 2013-11-01 | 2014-10-29 | 艾康生物技术(杭州)有限公司 | Nucleic acid extraction instrument |
| CN203661483U (en) * | 2013-12-31 | 2014-06-18 | 南阳飞龙电器有限公司 | Power supply cabinet inner plate fixing device |
| CN204369902U (en) * | 2014-12-09 | 2015-06-03 | 常州英德生物科技有限公司 | A kind of hold-down mechanism of multi-layer cellular culture plate |
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| CN104371922A (en) | 2015-02-25 |
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Effective date of registration: 20210622 Address after: 610000 South Sixth Road 669, Chengdu Economic and Technological Development Zone, Sichuan Province Patentee after: CHENGDU YINGDE BIO-MEDICAL EQUIPMENT TECHNOLOGY Co.,Ltd. Address before: 213000 d3010, No.26 Huashan Middle Road, Xinbei District, Changzhou City, Jiangsu Province Patentee before: CHANGZHOU YINGDE BIOTECHNOLOGY Co.,Ltd. |
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